Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,991,798 Δ1 bp 15.5% intergenic (+39/‑296) ygeG → / → ygeH SycD‑like chaperone family TPR‑repeat‑containing protein/putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,991,7980C.15.5% 316.9 / 56.8 110intergenic (+39/‑296)ygeG/ygeHSycD‑like chaperone family TPR‑repeat‑containing protein/putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (52/40);  new base . (7/10);  total (59/51)
Fisher's exact test for biased strand distribution p-value = 2.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.32e-01

Only 100 of 115 total aligned reads displayed.

TAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAG  >  NC_000913/2991699‑2991897
                                                                                                   |                                                                                                     
tAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGc                                                                                                       >  1:808820/1‑100 (MQ=255)
tAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGc                                                                                                       <  1:1832407/100‑1 (MQ=255)
tAAGAGTAAAGCGCAGGCATATATTCAAATCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGc                                                                                                       <  1:1274579/100‑1 (MQ=255)
   gagTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGt                                                                                                    <  2:570056/100‑1 (MQ=255)
   gagTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGt                                                                                                    >  2:2552786/1‑100 (MQ=255)
    agTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGtt                                                                                                   >  1:1299006/1‑100 (MQ=255)
     gTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCAATTGGTTAAATTTGCTGGTTATAGTGCAGt                                                                                                    >  1:2030077/1‑98 (MQ=255)
        aaGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAc‑‑c                                                                                             <  1:2202766/100‑1 (MQ=255)
          gtgcAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CAc                                                                                           <  1:1145178/98‑1 (MQ=255)
          gcgcAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CAc                                                                                           >  2:2500478/1‑100 (MQ=255)
                 cATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAAcc                                                                                    >  1:1469528/1‑100 (MQ=255)
                 cATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAAcc                                                                                    <  2:1146746/100‑1 (MQ=255)
                 cATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAAcc                                                                                    >  2:286446/1‑100 (MQ=255)
                 cATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACTGCACTATAAcc                                                                                    <  1:2519484/100‑1 (MQ=37)
                 cATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACTGCACTATAAcc                                                                                    <  2:52021/100‑1 (MQ=37)
                 cATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACTGCACTATAAcc                                                                                    <  2:404285/100‑1 (MQ=37)
                  atatatTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAAcc                                                                                    <  1:1647387/99‑1 (MQ=255)
                  atatatTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACTGCACTATAACCa                                                                                   <  2:741877/100‑1 (MQ=37)
                   tatatTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACTGCACTATAACCAg                                                                                  <  1:726195/100‑1 (MQ=37)
                   tatatTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACTGCACTATAACCAg                                                                                  <  1:1824424/100‑1 (MQ=37)
                   tatatTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACCGCACTATAAccgg                                                                                  <  2:1745646/100‑3 (MQ=37)
                    atatTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGc                                                                                 <  1:1462957/100‑1 (MQ=255)
                    atatTGAACTCTTAAAAGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGc                                                                                 <  1:219533/100‑1 (MQ=255)
                     tatTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCa                                                                                <  2:1126489/100‑1 (MQ=37)
                       atctaCTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCAcc                                                                              <  1:2526954/96‑1 (MQ=255)
                         gAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACctc                                                                            >  2:2490952/1‑100 (MQ=255)
                            ctctTAAACGATAATTCAGAAGATAATGGCTAAGCATTACGATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTa                                                                         >  2:1801470/1‑100 (MQ=255)
                            ctctTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTa                                                                         >  2:123705/1‑100 (MQ=255)
                              ctTAAACGATAATTCAGAAGATAATTGCTAATCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATg                                                                       <  1:457991/100‑1 (MQ=255)
                              ctTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATg                                                                       >  2:2478672/1‑100 (MQ=255)
                              ctTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATg                                                                       >  2:2029617/1‑100 (MQ=255)
                                 aaaCGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGttt                                                                    >  2:2183587/1‑100 (MQ=255)
                                    ttccgatctagaagaTAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCt                                                                 <  1:923358/91‑1 (MQ=255)
                                    cGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCt                                                                 >  2:598883/1‑100 (MQ=255)
                                    cGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCt                                                                 <  2:2520941/100‑1 (MQ=255)
                                    cGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCt                                                                 >  1:841655/1‑100 (MQ=255)
                                     gATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCtt                                                                <  2:2290684/100‑1 (MQ=255)
                                      aTAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTa                                                               >  2:657738/1‑100 (MQ=255)
                                      aTAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTa                                                               >  2:1960043/1‑100 (MQ=255)
                                       tAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTaa                                                              <  1:2188105/100‑1 (MQ=255)
                                         aTTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAAt                                                            >  1:38781/1‑100 (MQ=255)
                                          ttCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAAtt                                                           <  2:2581312/100‑2 (MQ=255)
                                           tCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCa                                                          >  2:2194304/1‑100 (MQ=255)
                                            cAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAg                                                         >  2:1532278/1‑100 (MQ=255)
                                             agaagaTAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCtagatcggaa                                                        >  2:923361/1‑91 (MQ=255)
                                             agaagaTAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCa                                                          <  1:1295200/98‑1 (MQ=255)
                                              gaagaTAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCa                                                       <  2:1657611/100‑1 (MQ=255)
                                               aagaTAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCa                                                       <  1:446175/99‑1 (MQ=255)
                                                agaTAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATc                                                     >  2:1441795/1‑100 (MQ=255)
                                                   tAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTa                                                  >  1:617380/1‑100 (MQ=255)
                                                     aTGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTaaa                                                >  2:1096055/1‑100 (MQ=255)
                                                     aTGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTaaa                                                <  2:830235/100‑1 (MQ=255)
                                                      tGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTaaaa                                               >  1:2426404/1‑100 (MQ=255)
                                                       ggCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAAc                                              <  2:2535587/100‑1 (MQ=255)
                                                        gCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACa                                             >  1:1008799/1‑100 (MQ=255)
                                                         cTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAg                                            <  2:136655/100‑1 (MQ=255)
                                                          tAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGt                                           >  2:1015533/1‑100 (MQ=255)
                                                          tAACCATTACAATCACTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CCCTCTAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGt                                           <  1:1543223/100‑1 (MQ=255)
                                                           aaGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGtt                                          <  1:1960033/100‑1 (MQ=255)
                                                           aaGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGtt                                          <  1:657735/100‑1 (MQ=255)
                                                           aaGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGtt                                          <  1:1961063/100‑1 (MQ=255)
                                                                ttACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAagag                                     <  2:2057821/100‑1 (MQ=255)
                                                                ttACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAagag                                     >  2:864867/1‑100 (MQ=255)
                                                                ttACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAagag                                     <  1:2415426/100‑1 (MQ=255)
                                                                 tACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGc                                    <  2:1282235/100‑1 (MQ=255)
                                                                  aCAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCa                                   >  1:1579343/1‑100 (MQ=255)
                                                                   cAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCAt                                  <  1:184607/100‑1 (MQ=255)
                                                                     aTCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATAc                                >  1:1094352/1‑100 (MQ=255)
                                                                     aTCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATAc                                <  2:2416558/100‑1 (MQ=255)
                                                                         tttGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACtata                            <  2:914923/100‑1 (MQ=255)
                                                                             gTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATc                        >  2:1321484/1‑100 (MQ=255)
                                                                             gTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATc                        >  2:489698/1‑100 (MQ=255)
                                                                                  aTTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCattat                   <  1:966340/100‑1 (MQ=255)
                                                                                  aTTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCattat                   <  1:761466/100‑1 (MQ=255)
                                                                                   tttGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCattatt                  <  2:941151/100‑1 (MQ=255)
                                                                                    gtGCTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTAttt                 >  1:1605623/2‑100 (MQ=25)
                                                                                      gCTGGTTATAGTGCAGTTAAC‑‑CACTATACCCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGAATACTATAAATAATTAttttt               >  2:201848/1‑100 (MQ=255)
                                                                                      gCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTAttttt               <  1:2561503/100‑1 (MQ=255)
                                                                                      gCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTAttttt               <  1:968888/100‑1 (MQ=255)
                                                                                      gCTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTAttttt               >  2:2079632/1‑100 (MQ=37)
                                                                                       cTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTc              <  1:598880/100‑1 (MQ=255)
                                                                                       cTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTc              <  1:764520/100‑1 (MQ=255)
                                                                                       cTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTAttttt               >  1:111488/1‑99 (MQ=37)
                                                                                       cTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTc              >  1:810244/1‑100 (MQ=37)
                                                                                       cTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTc              >  2:261913/1‑100 (MQ=37)
                                                                                        tGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCa             >  2:2330881/1‑100 (MQ=37)
                                                                                          gTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAAt           >  2:1280476/1‑100 (MQ=255)
                                                                                           ttATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTACAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATg          >  1:510740/1‑100 (MQ=255)
                                                                                           ttATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAAt           >  2:812744/1‑99 (MQ=255)
                                                                                           ttATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATg          >  2:607613/1‑100 (MQ=255)
                                                                                             ataGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCTATGc         >  1:928547/1‑99 (MQ=255)
                                                                                              taGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGt       >  2:1663417/1‑100 (MQ=255)
                                                                                               aGTGTAGTTAAC‑‑CACTATAACCACCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGtt      <  1:2372521/100‑1 (MQ=255)
                                                                                               aGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATATTATAAATCATTATTTTTAAATGCGtt      >  1:139367/1‑100 (MQ=255)
                                                                                               aGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGtt      <  1:2282726/100‑1 (MQ=255)
                                                                                                gTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTa     >  1:298828/1‑100 (MQ=255)
                                                                                                gTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTa     >  1:2570943/1‑100 (MQ=255)
                                                                                                 tGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTag    >  1:50242/1‑100 (MQ=255)
                                                                                                   cAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTagag  >  2:546601/1‑100 (MQ=255)
                                                                                                   cAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTagag  <  1:364352/100‑1 (MQ=255)
                                                                                                   |                                                                                                     
TAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAG  >  NC_000913/2991699‑2991897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: