Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,991,799 Δ1 bp 15.2% intergenic (+40/‑295) ygeG → / → ygeH SycD‑like chaperone family TPR‑repeat‑containing protein/putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,991,7990A.15.2% 315.9 / 52.7 112intergenic (+40/‑295)ygeG/ygeHSycD‑like chaperone family TPR‑repeat‑containing protein/putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (53/42);  new base . (7/10);  total (60/52)
Fisher's exact test for biased strand distribution p-value = 3.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.68e-01

Only 100 of 114 total aligned reads displayed.

GAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGA  >  NC_000913/2991702‑2991898
                                                                                                 |                                                                                                     
gagTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGt                                                                                                     <  2:570056/100‑1 (MQ=255)
gagTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGt                                                                                                     >  2:2552786/1‑100 (MQ=255)
 agTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGtt                                                                                                    >  1:1299006/1‑100 (MQ=255)
  gTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTa                                                                                                   >  2:764523/1‑100 (MQ=255)
  gTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCAATTGGTTAAATTTGCTGGTTATAGTGCAGt                                                                                                     >  1:2030077/1‑98 (MQ=255)
     aaGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAc‑‑c                                                                                              <  1:2202766/100‑1 (MQ=255)
       gtgcAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CAc                                                                                            <  1:1145178/98‑1 (MQ=255)
       gcgcAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CAc                                                                                            >  2:2500478/1‑100 (MQ=255)
            ggCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATaa                                                                                       >  2:506939/1‑100 (MQ=255)
              cATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAAcc                                                                                     <  2:1146746/100‑1 (MQ=255)
              cATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAAcc                                                                                     >  2:286446/1‑100 (MQ=255)
              cATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAAcc                                                                                     >  1:1469528/1‑100 (MQ=255)
              cATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACTGCACTATAAcc                                                                                     <  2:404285/100‑1 (MQ=37)
              cATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACTGCACTATAAcc                                                                                     <  2:52021/100‑1 (MQ=37)
              cATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACTGCACTATAAcc                                                                                     <  1:2519484/100‑1 (MQ=37)
               atatatTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAAcc                                                                                     <  1:1647387/99‑1 (MQ=255)
                tatatTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACTGCACTATAACCAg                                                                                   <  1:1824424/100‑1 (MQ=37)
                tatatTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACTGCACTATAACCAg                                                                                   <  1:726195/100‑1 (MQ=37)
                tatatTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACTGCACTATAACCAg                                                                                   <  2:2293488/100‑1 (MQ=37)
                tatatTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACCGCACTATAAccgg                                                                                   <  2:1745646/100‑3 (MQ=37)
                 atatTGAACTCTTAAAAGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGc                                                                                  <  1:219533/100‑1 (MQ=255)
                  tatTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCa                                                                                 <  2:1126489/100‑1 (MQ=37)
                   agtGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCa                                                                                 <  2:1126508/97‑1 (MQ=25)
                    atctaCTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCAcc                                                                               <  1:2526954/96‑1 (MQ=255)
                      gAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACctc                                                                             >  2:2490952/1‑100 (MQ=255)
                        aCTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCAccagat                                                                           >  2:2526973/1‑96 (MQ=255)
                        aCTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCtt                                                                           >  1:2516768/1‑100 (MQ=255)
                         ctctTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTa                                                                          >  2:123705/1‑100 (MQ=255)
                           ctTAAACGATAATTCAGAAGATAATTGCTAATCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATg                                                                        <  1:457991/100‑1 (MQ=255)
                           ctTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATg                                                                        >  2:2478672/1‑100 (MQ=255)
                           ctTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATg                                                                        >  2:2029617/1‑100 (MQ=255)
                              aaaCGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGttt                                                                     >  2:2183587/1‑100 (MQ=255)
                                 ttccgatctagaagaTAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCt                                                                  <  1:923358/91‑1 (MQ=255)
                                 cGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCt                                                                  >  2:598883/1‑100 (MQ=255)
                                 cGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCt                                                                  >  1:841655/1‑100 (MQ=255)
                                  gATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCtt                                                                 <  2:2290684/100‑1 (MQ=255)
                                   aTAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTa                                                                >  2:1961073/1‑100 (MQ=255)
                                   aTAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTa                                                                >  2:1960043/1‑100 (MQ=255)
                                   aTAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTa                                                                >  2:657738/1‑100 (MQ=255)
                                    tAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTaa                                                               <  1:2188105/100‑1 (MQ=255)
                                      aTTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAAt                                                             >  1:38781/1‑100 (MQ=255)
                                       ttCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAAtt                                                            <  2:2581312/100‑2 (MQ=255)
                                         cAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAg                                                          >  2:1532278/1‑100 (MQ=255)
                                          agaagaTAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCtagatcggaa                                                         >  2:923361/1‑91 (MQ=255)
                                          agaagaTAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCa                                                           <  1:1295200/98‑1 (MQ=255)
                                           gaagaTAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCa                                                        <  2:1657611/100‑1 (MQ=255)
                                            aagaTAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCa                                                        <  1:446175/99‑1 (MQ=255)
                                             agaTAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATc                                                      >  2:1441795/1‑100 (MQ=255)
                                                tAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTa                                                   >  1:617380/1‑100 (MQ=255)
                                                  aTGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTaaa                                                 <  2:830235/100‑1 (MQ=255)
                                                   tGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGTATCTTaaaa                                                >  1:880146/1‑100 (MQ=255)
                                                   tGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTaaaa                                                >  1:2426404/1‑100 (MQ=255)
                                                    ggCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAAc                                               <  2:2535587/100‑1 (MQ=255)
                                                     gCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACa                                              >  1:1008799/1‑100 (MQ=255)
                                                      cTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAg                                             <  2:136655/100‑1 (MQ=255)
                                                       tAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGt                                            >  2:1015533/1‑100 (MQ=255)
                                                       tAACCATTACAATCACTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CCCTCTAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGt                                            <  1:1543223/100‑1 (MQ=255)
                                                        aaGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGtt                                           <  1:1960033/100‑1 (MQ=255)
                                                        aaGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGtt                                           <  1:1961063/100‑1 (MQ=255)
                                                        aaGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGtt                                           <  1:657735/100‑1 (MQ=255)
                                                             ttACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAagag                                      >  2:864867/1‑100 (MQ=255)
                                                             ttACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAagag                                      <  2:2057821/100‑1 (MQ=255)
                                                             ttACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAagag                                      <  1:2415426/100‑1 (MQ=255)
                                                              tACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGc                                     <  2:1282235/100‑1 (MQ=255)
                                                               aCAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCa                                    >  1:1579343/1‑100 (MQ=255)
                                                                cAATCATTTGGTTAAATTTGCTGGTTTTAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCAt                                   <  1:637740/100‑1 (MQ=255)
                                                                  aTCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATAc                                 <  2:2416558/100‑1 (MQ=255)
                                                                  aTCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATAc                                 >  1:1094352/1‑100 (MQ=255)
                                                                      tttGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACtata                             <  2:914923/100‑1 (MQ=255)
                                                                          gTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATc                         >  1:1333541/1‑100 (MQ=255)
                                                                          gTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATc                         >  1:437131/1‑100 (MQ=255)
                                                                          gTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATc                         >  2:1321484/1‑100 (MQ=255)
                                                                               aTTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCattat                    <  1:761466/100‑1 (MQ=255)
                                                                               aTTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCattat                    <  1:966340/100‑1 (MQ=255)
                                                                                tttGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCattatt                   <  2:941151/100‑1 (MQ=255)
                                                                                 gtGCTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTAttt                  >  1:1605623/2‑100 (MQ=25)
                                                                                  tGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTAtttt                 <  1:204491/100‑1 (MQ=255)
                                                                                   gCTGGTTATAGTGCAGTTAAC‑‑CACTATACCCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGAATACTATAAATAATTAttttt                >  2:201848/1‑100 (MQ=255)
                                                                                   gCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTAttttt                <  1:2561503/100‑1 (MQ=255)
                                                                                   gCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTAttttt                <  1:968888/100‑1 (MQ=255)
                                                                                   gCTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTAttttt                >  2:2079632/1‑100 (MQ=37)
                                                                                    cTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTc               <  1:598880/100‑1 (MQ=255)
                                                                                    cTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTc               <  1:764520/100‑1 (MQ=255)
                                                                                    cTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTAttttt                >  1:111488/1‑99 (MQ=37)
                                                                                    cTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTAttttt                >  1:1764801/1‑99 (MQ=37)
                                                                                    cTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTc               >  2:261913/1‑100 (MQ=37)
                                                                                     tGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCa              >  2:2330881/1‑100 (MQ=37)
                                                                                       gTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAAt            >  2:1280476/1‑100 (MQ=255)
                                                                                        ttATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAAt            >  2:812744/1‑99 (MQ=255)
                                                                                        ttATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATg           >  2:607613/1‑100 (MQ=255)
                                                                                          ataGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCTATGc          >  1:928547/1‑99 (MQ=255)
                                                                                           taGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGt        >  2:1663417/1‑100 (MQ=255)
                                                                                            aGTGTAGTTAAC‑‑CACTATAACCACCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGtt       <  1:2372521/100‑1 (MQ=255)
                                                                                            aGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATATTATAAATCATTATTTTTAAATGCGtt       >  1:139367/1‑100 (MQ=255)
                                                                                             gTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTa      >  1:298828/1‑100 (MQ=255)
                                                                                              tGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTag     >  1:50242/1‑100 (MQ=255)
                                                                                                cAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTagag   >  2:546601/1‑100 (MQ=255)
                                                                                                cAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTagag   <  1:364352/100‑1 (MQ=255)
                                                                                                 aGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAgaga  <  2:1706276/100‑1 (MQ=255)
                                                                                                 aGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAgaga  >  2:1424061/1‑100 (MQ=255)
                                                                                                 |                                                                                                     
GAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGA  >  NC_000913/2991702‑2991898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: