Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,427,068	Δ1 bp	100%	intergenic (‑35/+8)	alaU ← / ← ileU	tRNA‑Ala/tRNA‑Ile

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,427,068	0	T	.	33.3%	‑7.0 / 2.6	3	intergenic (‑35/+8)	alaU/ileU	tRNA‑Ala/tRNA‑Ile
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base . (0/1);  minor base G (0/1);  total (1/2)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGC‑CGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACC  >  NC_000913/3426977‑3427107                                                                                             |                                        cGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGC‑ACGGCAAAttt                                 <  1:252723/100‑1 (MQ=1)  gAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCACG‑GCAAAtttn                                <  2:2475413/100‑2 (MQ=1)                                     accaaGCAGAGCTAAAGCACCATACCATGTAGTTAAAACCTCTGCAAATTTGA‑AATGGAAAATTGCTAGGCCTGAGTGGAATTGAAGCACAGAcc  >  1:2011486/5‑95 (MQ=0)                                                                                             |                                        CGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGC‑CGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACC  >  NC_000913/3426977‑3427107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑