Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,756,565 Δ1 bp 15.2% intergenic (‑54/+111) aldB ← / ← yiaY aldehyde dehydrogenase B/L‑threonine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,756,5650C.15.2% 340.0 / 63.0 125intergenic (‑54/+111)aldB/yiaYaldehyde dehydrogenase B/L‑threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (58/43);  new base . (12/7);  total (71/54)
Fisher's exact test for biased strand distribution p-value = 8.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.99e-01

Only 100 of 129 total aligned reads displayed.

CATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGT  >  NC_000913/3756466‑3756664
                                                                                                    |                                                                                                   
cATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATc                                                                                                     <  2:95387/100‑1 (MQ=255)
cATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATc                                                                                                     >  2:1960435/1‑100 (MQ=255)
  tACTCTCCGGGCTTAATCTGTGCTGAAGTGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTTAGGTATAACAAGGGTAGACGTGACTTGCGAAATCtt                                                                                                   <  1:357444/100‑1 (MQ=255)
    cTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCg                                                                                                 >  2:597310/1‑100 (MQ=255)
    cTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCg                                                                                                 <  2:517933/100‑1 (MQ=255)
    cTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCg                                                                                                 <  2:2033025/100‑1 (MQ=255)
    cTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCg                                                                                                 <  2:1569744/100‑1 (MQ=255)
    cTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCg                                                                                                 >  1:2346498/1‑100 (MQ=255)
    cTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGCTAGACGTGACTGGCGAAATCTTCg                                                                                                 <  1:1895443/100‑1 (MQ=255)
      cGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGcc                                                                                               <  2:1553028/100‑1 (MQ=255)
       gCCGGGCTTAATCTGTTCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCa                                                                                              >  1:1014054/1‑100 (MQ=255)
        ccGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAGATTTCGCCAg                                                                                             <  2:1379724/100‑1 (MQ=255)
        ccGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAg                                                                                             <  2:309195/100‑1 (MQ=255)
         cGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAGATTTCGCCAGt                                                                                            <  1:1247525/100‑1 (MQ=255)
          gggCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAGATTTCGCCAGTc                                                                                           <  2:1834181/100‑1 (MQ=255)
           ggCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCg                                                                                          <  2:1339004/100‑1 (MQ=255)
           ggCGTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAGATTTCGCCAGTca                                                                                          <  1:2072743/100‑2 (MQ=255)
           ctcTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGCGACTGGCGAAGATTTCGCCAGTca                                                                                          <  1:1525030/98‑2 (MQ=255)
            gCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAGATTTCGCCAGTcac                                                                                         <  2:2119933/100‑3 (MQ=255)
            gCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAGATTTCGCCAGTcan                                                                                         <  1:1071373/100‑3 (MQ=255)
             cTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAGATTTCGCCAGTcacg                                                                                        <  1:518390/100‑4 (MQ=39)
             cTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGt                                                                                        >  1:1848375/1‑100 (MQ=255)
             cTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGCGACTGGCGAAGATTTCGCCAGTcacg                                                                                        <  1:1573503/100‑4 (MQ=38)
               tAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTaa                                                                                      <  2:256064/100‑1 (MQ=255)
                aaTCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAAc                                                                                     >  2:438360/1‑100 (MQ=255)
                      tgCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCttt                                                                               <  1:1732465/100‑1 (MQ=255)
                      tgCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCttt                                                                               >  2:1170812/1‑100 (MQ=255)
                         tGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACg                                                                            <  2:1981354/100‑1 (MQ=255)
                         tGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACg                                                                            >  2:1274368/1‑100 (MQ=255)
                          gAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGa                                                                           >  2:1728767/1‑100 (MQ=255)
                             gggggATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAg                                                                        >  2:781326/1‑100 (MQ=255)
                              ggtgATTATTAGTCATGATCGTCTCCATGCGGTGTGAGGTATAACAAGGGTAGAAGTGACTAGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGATAg                                                                        <  1:1391000/96‑1 (MQ=255)
                               gggATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGAGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCt                                                                      >  1:1741173/1‑100 (MQ=255)
                                  attattGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATc                                                                   <  1:1845857/100‑1 (MQ=255)
                                   ttattGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCa                                                                  <  2:1628158/100‑1 (MQ=255)
                                    ccgatctCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCAc                                                                 <  2:84527/94‑1 (MQ=255)
                                     attGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACg                                                                >  1:2305875/1‑100 (MQ=255)
                                      ttGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGa                                                               >  2:1598103/1‑100 (MQ=255)
                                      ttGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGa                                                               >  2:2219927/1‑100 (MQ=255)
                                       tGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGaa                                                              >  2:176331/1‑100 (MQ=255)
                                        ggTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAAt                                                             >  2:1903335/1‑100 (MQ=255)
                                           cATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCAcagatcgg                                                          >  1:84526/1‑93 (MQ=255)
                                           cATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTa                                                          >  2:1145308/1‑100 (MQ=255)
                                             tGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACg                                                        >  1:2309044/1‑100 (MQ=255)
                                             tGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACg                                                        <  2:63426/100‑1 (MQ=255)
                                              gATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACgg                                                       >  2:1566610/1‑100 (MQ=255)
                                                tcgtcgCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGc                                                     <  1:183077/100‑1 (MQ=255)
                                                 cGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCa                                                    >  2:2148056/1‑100 (MQ=255)
                                                      ccTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGt                                               >  1:277445/1‑100 (MQ=255)
                                                       cTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGtt                                              <  1:699521/100‑1 (MQ=255)
                                                        ttGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTa                                             <  1:368707/100‑1 (MQ=255)
                                                         tGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTAc                                            >  2:424140/1‑100 (MQ=255)
                                                           cGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACag                                          >  2:1264512/1‑100 (MQ=255)
                                                            ggTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACATCTATCACGAATTTACGGTCAAGGGTTACaga                                         <  2:1743185/100‑1 (MQ=255)
                                                            ggTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACaga                                         <  1:457467/100‑1 (MQ=255)
                                                            ggTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACaga                                         <  2:1953025/100‑1 (MQ=255)
                                                             gtgtGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACagag                                        >  1:2092937/1‑100 (MQ=255)
                                                                tgAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATa                                     <  2:1474978/100‑1 (MQ=255)
                                                                tgAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATa                                     >  2:2276964/1‑100 (MQ=255)
                                                                   ggTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGtt                                  <  2:1472600/100‑1 (MQ=255)
                                                                   ggTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGtt                                  >  2:1999980/1‑100 (MQ=255)
                                                                   ggTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGtt                                  >  2:51715/1‑100 (MQ=255)
                                                                   ggTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGtt                                  >  1:1252907/1‑100 (MQ=255)
                                                                    gTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTg                                 >  1:1011535/1‑100 (MQ=255)
                                                                     tataACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGa                                <  1:2534689/100‑1 (MQ=255)
                                                                     tataACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGa                                >  2:422777/1‑100 (MQ=255)
                                                                       taACAAGGGTAGACGTGACTGGCGAAATCTTCACCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGaaa                              >  2:2459859/1‑100 (MQ=255)
                                                                        agcAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGaaaa                             <  1:453822/98‑1 (MQ=255)
                                                                        aaCAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGaaaa                             >  2:1890463/1‑100 (MQ=255)
                                                                        aaCAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGaaaa                             <  1:1028906/100‑1 (MQ=255)
                                                                        aaCAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGaaaa                             >  2:323622/1‑100 (MQ=255)
                                                                         aCAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGaaaaa                            >  2:1112975/1‑100 (MQ=255)
                                                                             gggTAGACGTGACTGGCGAAATCTTAGACAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCg                         <  2:354033/99‑1 (MQ=255)
                                                                              ggTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGc                       <  1:51714/100‑1 (MQ=255)
                                                                                   taccgACTGGCGAAGATTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTAGAGAGATAGTTGAAAAAGCGGCTAACa                  >  1:663000/5‑100 (MQ=38)
                                                                                    cGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACaa                 >  1:1286849/1‑100 (MQ=255)
                                                                                     ccgACTGGCGAAGATTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAAt                >  1:1652687/3‑100 (MQ=255)
                                                                                     ccgACTGGCGAAGATTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAAt                >  1:2118583/3‑100 (MQ=255)
                                                                                      tGACTGGCGAAGATTTCCCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAAtt               >  1:849060/1‑100 (MQ=255)
                                                                                       gACTGGCGAAGATTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAAttt              >  2:2323979/1‑100 (MQ=255)
                                                                                       gACTGGCGAAGATTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAAttt              >  2:2400551/1‑100 (MQ=255)
                                                                                        acgggCGGAATCTCCGCCAGTAGGTAACAGCTATAGAACAGGTATCACGAATTAACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTg             <  1:700374/97‑1 (MQ=255)
                                                                                        aCTGGCGAAGATTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTg             >  2:2352872/1‑100 (MQ=255)
                                                                                        aCTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTg             <  2:2007336/100‑1 (MQ=255)
                                                                                         cTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGc            >  2:430875/1‑100 (MQ=255)
                                                                                         cTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGc            >  2:738520/1‑100 (MQ=255)
                                                                                         cTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGc            >  2:1643704/1‑100 (MQ=255)
                                                                                         cTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGc            >  2:485111/1‑100 (MQ=255)
                                                                                         cTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGc            >  2:429818/1‑100 (MQ=255)
                                                                                          tGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGcc           <  2:2095012/100‑1 (MQ=255)
                                                                                          tGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGcc           <  2:1520165/100‑1 (MQ=255)
                                                                                           ggCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCa          >  2:542199/1‑100 (MQ=255)
                                                                                           ggCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCa          >  2:2278008/1‑100 (MQ=255)
                                                                                            gCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAg         >  1:879519/1‑100 (MQ=255)
                                                                                             cGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGc        >  2:46871/1‑100 (MQ=255)
                                                                                              gAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGcc       >  1:1525833/1‑100 (MQ=255)
                                                                                                aaTCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGt     >  2:608994/1‑100 (MQ=255)
                                                                                                  aTCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGtt    <  2:166412/100‑1 (MQ=255)
                                                                                                    cTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTtgt  >  2:1012275/1‑100 (MQ=255)
                                                                                                    cTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTtgt  >  2:19374/1‑100 (MQ=255)
                                                                                                    |                                                                                                   
CATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGT  >  NC_000913/3756466‑3756664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: