Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,410,053 (T)9→8 100% intergenic (+47/‑80) rlmB → / → yjfI 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,410,0450T.95.9% 401.0 / 9.2 98intergenic (+39/‑88)rlmB/yjfI23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base . (48/46);  total (51/47)
Fisher's exact test for biased strand distribution p-value = 6.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

CCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATG  >  NC_000913/4409946‑4410140
                                                                                                   |                                                                                               
ccTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtt                                                                                                >  1:268687/1‑100 (MQ=255)
  tGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttt                                                                                               <  2:2391293/100‑1 (MQ=255)
  tGAACGTGTCGGTTGCGACCGGTATTTGCTTATATGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAAATCTGGATGGctt                                                                                               <  1:2408755/100‑1 (MQ=255)
    aaCGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttt                                                                                              >  2:2433445/1‑99 (MQ=255)
      cGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttt                                                                                           <  2:852548/100‑1 (MQ=255)
      cGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttt                                                                                           >  2:1739927/1‑100 (MQ=255)
       gTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGGcttttcat                                                                                          <  2:1028889/100‑4 (MQ=37)
       gTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAACTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGGcttttcat                                                                                          <  2:727954/100‑4 (MQ=37)
        tttCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttg                                                                                         <  1:2523190/100‑2 (MQ=255)
        tttCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttg                                                                                         <  2:2248954/100‑2 (MQ=255)
         ttCGGTCGCGACCGGAATTTGCTTATTTGAATCGGTGCGCCAGCGCAGCTAATTTCTCATAATTATGAAAAACCATCCAAATCTGGATGGcttttca                                                                                           <  1:1763485/97‑3 (MQ=25)
          tCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgtc                                                                                       <  1:2352233/100‑4 (MQ=255)
          tCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgtc                                                                                       <  2:2099963/100‑4 (MQ=255)
          tCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgt                                                                                        >  1:2180511/1‑97 (MQ=255)
           cGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgtct                                                                                      >  2:2114790/1‑96 (MQ=255)
           cGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgtct                                                                                      <  1:2561538/100‑5 (MQ=255)
           cGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGGcttttcataatt                                                                                      <  1:1862933/100‑8 (MQ=25)
            ggTTGCGACCGGAATTTGCTTATTTGTAGCTTTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGGcttttcataattc                                                                                     <  1:1730953/100‑9 (MQ=21)
            ggTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCta                                                                                    >  2:1489052/1‑100 (MQ=255)
            ggTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCta                                                                                    >  1:110390/1‑100 (MQ=255)
            ggTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGGcttttcataattc                                                                                     <  2:1946639/100‑9 (MQ=25)
             gTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCtat                                                                                   <  1:2088872/100‑1 (MQ=255)
              ttGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCtata                                                                                  <  2:1492072/100‑1 (MQ=255)
               tGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAg                                                                                 >  1:1628861/1‑100 (MQ=255)
                gCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAg                                                                                 >  2:2046506/1‑100 (MQ=255)
                 cGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCt                                                                               <  2:1155993/100‑1 (MQ=255)
                 cGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCt                                                                               >  2:2517166/1‑100 (MQ=255)
                 cGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCt                                                                               <  1:1046874/100‑1 (MQ=255)
                   aCCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTgg                                                                             >  1:1018369/1‑100 (MQ=255)
                   aCCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTg                                                                              >  1:709694/1‑99 (MQ=255)
                   aCCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGCATGGTTTTTTTTGTCTATAGCTg                                                                              >  1:2157929/1‑99 (MQ=255)
                     cGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTa                                                                           <  2:1396882/100‑1 (MQ=255)
                       gAATTTTCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTaa                                                                          <  1:1885220/99‑1 (MQ=255)
                       gAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCATAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAg                                                                         <  1:1825361/100‑1 (MQ=255)
                       gAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAg                                                                         <  1:463873/100‑1 (MQ=255)
                         aTTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGAt                                                                       >  1:224775/1‑100 (MQ=255)
                          tttGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTa                                                                           <  2:2341281/95‑1 (MQ=255)
                          tttGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATa                                                                      >  2:2279525/1‑100 (MQ=255)
                          ttagCTGATTTGAAGCGGTGCGCCAGCGCAGTGAATTTCTCAGAATTATGAAAAGCCATCCAGATTTTGATGGTTTTTTTTGTCTATAGCTGGTAAg                                                                         <  1:1286645/94‑1 (MQ=255)
                             gCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAAtt                                                                   <  2:1179905/100‑1 (MQ=255)
                             gCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAAtt                                                                   >  2:704041/1‑100 (MQ=255)
                              catATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTa                                                                  >  2:1758334/3‑100 (MQ=255)
                                tATTTGAAGCGGTGCGCCAGCGAAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTATGATAAtt                                                                   >  1:2050305/1‑97 (MQ=255)
                                  tttGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTa                                                              >  2:2386415/1‑100 (MQ=255)
                                   ttGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTAt                                                             >  2:565219/1‑100 (MQ=255)
                                       aGCGTTGCGCCAACGCAACTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTTTAGCTGGTAAGGTAATTACGTAtt                                                            >  1:6616/1‑98 (MQ=255)
                                         cGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGTaaa                                                       <  2:2558940/100‑1 (MQ=255)
                                         cGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCaaa                                                       >  2:461620/1‑100 (MQ=255)
                                           gTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAata                                                     <  2:518685/100‑1 (MQ=255)
                                            tGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTTTCTAGCTGGTAAGATAATTACGCCTTGCAAatat                                                    >  1:2462434/1‑100 (MQ=255)
                                              cgcCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTTCAAATATTc                                                  >  1:1734251/1‑100 (MQ=255)
                                              cgcCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATAtt                                                   >  1:2385551/1‑99 (MQ=255)
                                               gcCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCa                                                 <  1:1250472/100‑1 (MQ=255)
                                                ccAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAt                                                >  1:745320/1‑100 (MQ=255)
                                                ccAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAt                                                >  2:1923365/1‑100 (MQ=255)
                                                 cAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAtt                                               >  2:2533248/1‑100 (MQ=255)
                                                  aGCCCATCTAATTTCTCTTAATTATGAACAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCa                                                 <  1:1427796/97‑1 (MQ=255)
                                                     gcAGCTAATTTCTCATAATTATGAAAAGCCATCCAGATTTGAATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttat                                           <  1:226628/100‑1 (MQ=255)
                                                     gcAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttat                                           <  1:2115533/100‑1 (MQ=255)
                                                     gcAGATAATTTCGCAGAAGTATGAAAAGCCATCGAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttat                                           <  1:1248932/100‑1 (MQ=255)
                                                      cAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttatt                                          <  2:2315893/100‑1 (MQ=255)
                                                       aGCTGATTGCGCAGGATTATGAAAAGCCATCGAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttatta                                         <  2:1517929/100‑1 (MQ=255)
                                                       aGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATTTTTTTTTTTTTATTTACGTATTGCAAATATTCATTttatta                                         >  1:2294716/1‑100 (MQ=255)
                                                        gCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTaa                                        <  2:2230212/100‑1 (MQ=255)
                                                         cTAATTTCTCAGAATTATGAAAAGCCATCAAGATGTGTATGGTTTTTTTTGTATATAGCTGGTAAGATAATTACGTATTGCAAATGTTTATTTTATTaa                                        >  1:1377269/1‑99 (MQ=255)
                                                          tAATTTTTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAat                                       <  1:2561771/99‑1 (MQ=255)
                                                          tAATTTCTCAGAATTATGAAAATCCATCCAGATTTGGATGGTTTTTTTTGTTTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAata                                      <  1:1958803/100‑1 (MQ=255)
                                                          tAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAata                                      >  2:1054780/1‑100 (MQ=255)
                                                          tAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAata                                      <  2:2314546/100‑1 (MQ=255)
                                                          tAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAat                                       <  1:1293795/99‑1 (MQ=255)
                                                             tttCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATAttt                                   <  2:2165875/100‑1 (MQ=255)
                                                                 tcAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAAc                               >  1:30211/1‑100 (MQ=255)
                                                                 tcAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAAc                               <  2:2118822/100‑1 (MQ=255)
                                                                   aGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACtt                             >  1:368107/1‑100 (MQ=255)
                                                                    gAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTTGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACt                              <  1:1210026/98‑1 (MQ=255)
                                                                      aTTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAAt                          <  2:461567/100‑1 (MQ=255)
                                                                      aTTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAAt                          <  1:28229/100‑1 (MQ=255)
                                                                       ttATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACtt                             <  1:1110095/96‑1 (MQ=255)
                                                                        caaaaaaaACCATCCAAATCTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCt                        >  1:2433830/5‑100 (MQ=37)
                                                                        caaaaaaaACCATCCAAATCTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTAATTTTATTAATAATTAAACTTAATCt                        >  1:887544/5‑100 (MQ=37)
                                                                        caaaaaaaACCATCCAAATCTGGATGGCTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCt                        >  2:685078/5‑100 (MQ=25)
                                                                         aaaaaaaACCATCCAAATCTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCta                       >  2:1172862/4‑100 (MQ=38)
                                                                         aTGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTACACTTAATCta                       >  2:2474885/1‑100 (MQ=255)
                                                                          tGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTTTTTTTTTTAATAAGAAAATTAGTTATTGTATTTTTTCGTTTTATTGATTTTTAAACTTAATCttt                      >  1:636626/1‑98 (MQ=255)
                                                                          tGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTTTATTGCTGGTAAGATAATTACGTATTGCAAATATTCTTTTTATTAATATTTATACTTAATCt                        >  1:516704/1‑98 (MQ=255)
                                                                          tGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATACTTAGGTATTGCAAATAGTAATTCTCTTAATATTTACACTTAATCtat                      >  1:2122062/1‑100 (MQ=255)
                                                                          aaaaaaACCATCCAAATCTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCtat                      >  1:1477805/3‑100 (MQ=38)
                                                                           aaaaaACCATCCAAATCTGGATGGTTTTTTTTGTCTATAGCTTGTAAGATAATTACGAATTGCAAATATTCATTTTATTAATATTTATACTTAATCtata                     >  1:1206940/2‑100 (MQ=37)
                                                                            aaaaGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTatat                    >  1:82609/1‑100 (MQ=255)
                                                                            aaaaACCATCCAAATCTGGATGGTTTTTTTTGTCTATATCTGGTAAGATAATTACGTATTTCAAATATTCATTTTATTAATATTTAAACTTAATCTatat                    >  1:910945/1‑100 (MQ=255)
                                                                              aaGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATgg                  <  2:530181/100‑1 (MQ=255)
                                                                                  cctCCATATTTGGATGGTTTTTTTTGTCTATAGCTGGTAATATAATTACTTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaa                <  1:1670935/96‑1 (MQ=255)
                                                                                    tCCAAATCTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaataat            >  2:208301/1‑100 (MQ=255)
                                                                                     ccAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAataata           <  1:1319807/100‑1 (MQ=255)
                                                                                       aGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatata         <  2:1552859/100‑1 (MQ=255)
                                                                                        gATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatatat        >  2:1010532/1‑100 (MQ=255)
                                                                                         aTTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATg       <  1:104601/100‑1 (MQ=255)
                                                                                           tttgATGGTTTTTTTTGTCTATTGATTGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAataata           <  1:2088188/91‑1 (MQ=255)
                                                                                             ggATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACAt   >  2:1677132/1‑100 (MQ=255)
                                                                                              gATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATg  <  2:1122551/100‑1 (MQ=255)
                                                                                                   |                                                                                               
CCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATG  >  NC_000913/4409946‑4410140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: