| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,850,719:1 | +T | 16.0% | intergenic (+26/‑141) | sppA → / → ansA | protease IV (signal peptide peptidase)/cytoplasmic L‑asparaginase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,850,719 | 1 | . | T | 16.0% | 221.8 / 47.2 | 75 | intergenic (+26/‑141) | sppA/ansA | protease IV (signal peptide peptidase)/cytoplasmic L‑asparaginase 1 |
| Reads supporting (aligned to +/- strand): ref base . (30/33); new base T (5/7); total (35/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
GTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCA > NC_000913/1850620‑1850817 | gtgaCAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGAtc < 1:2021792/100‑1 (MQ=255) aCAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGAtc‑gt > 2:398370/1‑99 (MQ=255) aaGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCgg < 2:1081386/100‑1 (MQ=255) gCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGcc < 1:73827/100‑1 (MQ=255) cTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGcc < 1:2494515/99‑1 (MQ=255) tGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTATTGAGTGGCCGAC‑GATCTGTCGGCcac < 2:1575202/100‑1 (MQ=255) tGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGAC‑GATCTGTCGGCcac < 1:1000953/100‑1 (MQ=255) tGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGAC‑GATCTGTCGGCcac < 2:894815/100‑1 (MQ=255) tGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGAC‑GATCTGTCGGCcac < 1:2001851/100‑1 (MQ=255) ggCCGCGTTTAGTGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCgg < 1:774920/95‑1 (MQ=255) gCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGAC‑GATCTGTCGGCcactc < 2:1080229/100‑3 (MQ=255) ccGCGTTTAATGACCCACAAAACCGTTATGCGAATTGCCTGACCTGCGCCCAAATGCGTTAAGACCTGTAATGAGTGGCAGACAGATA‑GTCGGCcaca > 1:806427/1‑98 (MQ=255) ncgTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACattat > 1:1266566/2‑100 (MQ=255) cgTTTAATGACCCACAAAACCGTTATGTGTTTTGACTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGAC‑GATCTGTCGGCcactcagta < 1:616735/100‑7 (MQ=37) ttAATGACCCACAAAACCGTTATGCTTTTTGCCTGACTTGCGGCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTAttttc < 1:791424/100‑2 (MQ=255) ttAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTAttttt < 2:16651/100‑1 (MQ=255) ttAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTAttttt < 2:1774938/100‑1 (MQ=255) tAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTAtttttt > 1:2020434/1‑100 (MQ=255) tAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGAC‑GATCTGTCGGCcactcagtacaag < 1:1719495/100‑11 (MQ=37) aggACCCACAAAAACGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTAc > 2:1757270/3‑100 (MQ=255) aTGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTGCTGAGTGGCCGACAGATC‑GTCGGCCACATTAtttttt > 2:2157619/1‑98 (MQ=255) aTGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTAc < 2:1316493/100‑1 (MQ=255) tGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACg < 2:1269116/100‑1 (MQ=255) gACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGt > 1:1228069/1‑100 (MQ=255) cccACAAAACCGTTATGCGGTTTGCCTGACCTGCGCCAACATGCGGTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTcg > 1:400757/1‑100 (MQ=255) cacaAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGCGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTcgac < 1:2172654/100‑1 (MQ=255) acaAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTcgacg < 2:318452/100‑1 (MQ=255) aaaaCCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGaa < 2:2286909/100‑1 (MQ=255) ccGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGATTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATcc < 1:1436005/99‑1 (MQ=255) ccGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCct > 2:2310456/1‑100 (MQ=255) cGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCctc < 2:2060137/100‑1 (MQ=255) ntGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTcc > 1:881875/2‑100 (MQ=255) tGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCAAATTATTTTTTACGTCGACGAATCCTCTTccc > 2:1717471/1‑100 (MQ=255) ttttGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTg < 2:374881/100‑1 (MQ=255) ttttGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTg > 1:770620/1‑100 (MQ=255) tttGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGt < 2:930350/100‑1 (MQ=255) tGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGttt > 1:2278879/1‑100 (MQ=255) gCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTc > 2:185566/1‑100 (MQ=255) ccTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCg > 2:120249/1‑100 (MQ=255) tGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGcc < 1:347106/100‑1 (MQ=255) tGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGcc < 2:896412/100‑1 (MQ=255) gACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGccc < 2:1383537/100‑1 (MQ=255) aCCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGcccc < 1:733559/100‑1 (MQ=255) ccTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCa > 1:2229169/1‑100 (MQ=255) tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCata < 1:418009/100‑1 (MQ=255) gcgcCAACATGCGTCAAGTCTCGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGCTTCCCCCCctat > 1:898300/1‑100 (MQ=255) cgcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATAtt < 1:254882/100‑1 (MQ=255) gcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATAttt < 1:89175/100‑1 (MQ=255) ccAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTc < 2:2027277/100‑1 (MQ=255) ccAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTc > 2:2091567/1‑100 (MQ=255) ccAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTc > 2:2480933/1‑100 (MQ=255) cAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTcc < 1:2076930/100‑1 (MQ=255) tGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATc < 1:2091553/100‑1 (MQ=255) gCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATcc > 2:933798/1‑100 (MQ=255) cGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCg > 2:363873/1‑100 (MQ=255) cGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCg < 1:120248/100‑1 (MQ=255) tAACTCTTTTACTGCTTGGCCGACAGCTC‑GTCGGCCACATTATTTTTTACGTTGACTAACCCTCTTCCTTCTGTTTCGCCCCATATTTCCTTATCCg < 1:2533273/97‑1 (MQ=255) gTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGt > 2:935172/1‑100 (MQ=255) gTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGt > 2:2033583/1‑100 (MQ=255) gTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGt > 1:1858626/1‑100 (MQ=255) tCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCTGCAt > 1:807516/1‑96 (MQ=255) tCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTa < 2:2104232/100‑1 (MQ=255) aaaataatgTGGCCGAC‑GATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCa > 2:811259/9‑100 (MQ=37) aaataatgTGGCCGAC‑GATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAg > 2:2377046/8‑100 (MQ=38) tACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAgg < 1:268394/100‑1 (MQ=255) aCTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTCTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGt < 1:2045429/100‑1 (MQ=255) aatgTGGCCGAC‑GATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTgc > 2:2275985/4‑100 (MQ=39) gAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTgcg < 2:2323237/100‑1 (MQ=255) gTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCt > 1:932965/1‑100 (MQ=255) gTGGCCGAC‑GATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTgcgc > 1:959483/1‑99 (MQ=255) tGGCCGAC‑GATCTGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTc > 2:2283270/1‑100 (MQ=255) ggCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTcc > 2:2338341/1‑100 (MQ=255) gCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATAGTTCCTTATCCGCATAGTATCAGGTGCGCTccc > 2:762886/1‑100 (MQ=255) cAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGc > 1:2180401/1‑100 (MQ=255) cAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGc > 2:210153/1‑100 (MQ=255) aGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGcc < 2:1390462/100‑1 (MQ=255) aTC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTc > 2:2091458/1‑100 (MQ=255) tc‑gtcgGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCa > 2:1041894/1‑100 (MQ=255) tc‑gtcgGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCa < 2:769209/100‑1 (MQ=255) tc‑gtcgGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCa > 2:120741/1‑100 (MQ=255) | GTGACAAGCTGGCCGCGTTTAATGACCCACAAAACCGTTATGCGTTTTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATC‑GTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCA > NC_000913/1850620‑1850817 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |