| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,185,875:1 | (G)7→8 | 100% | intergenic (‑79/‑42) | rcnR ← / → rcnA | transcriptional repressor of rcnA/membrane protein conferring nickel and cobalt resistance |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,185,868 | 1 | . | G | 66.0% | 101.2 / 61.6 | 50 | intergenic (‑72/‑49) | rcnR/rcnA | transcriptional repressor of rcnA/membrane protein conferring nickel and cobalt resistance |
| Reads supporting (aligned to +/- strand): ref base . (11/6); new base G (31/1); total (42/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.84e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTT > NC_000913/2185771‑2185963 | tGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAA‑AAATCACAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑‑g < 2:1795422/99‑1 (MQ=255) gTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAA‑AAATCATAGCACTATTAAACTACTGGGGGGTAGTATCAGGTACT‑‑g‑gg > 2:861079/1‑100 (MQ=255) gTTTATCACGGATTGTATGAGACATGGCAACACCTGATTAACAAGAATATGAA‑AAATCATAGCACGATTAATCTACTGGGGGGTAGTATCAGGTACT‑‑g‑g > 1:14328/1‑99 (MQ=255) tttATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑‑g‑ggg > 2:1797098/1‑100 (MQ=255) ttATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑‑g‑ggg‑g > 2:2157046/1‑100 (MQ=255) aTCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑‑g‑ggg‑gg‑g > 1:253530/1‑100 (MQ=255) cACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑‑g‑ggg‑gg‑gga > 2:862515/1‑98 (MQ=255) cACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑‑g‑ggg‑gg‑gga > 2:2095808/1‑98 (MQ=255) aCGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑‑g‑ggg‑gg‑ggag > 1:2416540/1‑97 (MQ=255) aCGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACC‑‑g‑ggg‑gg‑gg > 1:1500736/1‑97 (MQ=255) gATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑G‑GGG‑GG‑G‑AGTAg > 2:2242049/1‑100 (MQ=255) gTATGAGACATGGCAACACCTGGTTAACAAGAATATGAA‑AAATCATAGCATTATTGATTTGCTGGGTGGTAGACTCAGGTACT‑‑g‑ggg‑gg‑ggagt < 1:125972/94‑5 (MQ=255) gTATGAGACATGGCAACACCTGGTTAACAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATc > 1:998415/1‑100 (MQ=255) gTATGAGACATGGCAACACCTGGTTAACAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑‑G‑GGG‑GG‑G‑AGTAGAATCa > 1:1549361/1‑100 (MQ=255) tATGATAAATGACAACACTTGCTTAATAAGTTTTTGAT‑AAATCATAGTCGTATTGGGTTACTGGGGGGTAGTTTCAGGTTTTTGG‑GGG‑GG‑G‑AGTAGAATc < 1:2009230/100‑1 (MQ=255) aTGAGACATGGCAACACCAGACTAACAAGAAGATGAA‑AAATCAAAGCCCGATTAATATACTGAAGGGTAGTATAAGGAACT‑‑G‑GGG‑GG‑GAAGTAGAAt < 1:1118882/97‑1 (MQ=255) tGAGACATGGCAACACTTGGTTAACAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGa > 2:721180/1‑100 (MQ=255) agaCATGGCAACACCTGGTTAACAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGAtt > 1:1719657/1‑100 (MQ=255) aTGGCAACACCTGGTTAACAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCg > 2:1477062/1‑100 (MQ=255) ggCAACACCTGGTTAACAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGaa > 2:1293757/1‑100 (MQ=255) ggCAACACCTGGTTAACAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGAGGGTAGTATCAGGTACT‑‑G‑GGG‑GGTG‑AGTAAAATCAGATTGCCGaa > 1:826469/1‑100 (MQ=255) gaaaCACCTGGTTACCAA‑AATAGAAACAAATCATAGCCCTATTGCTCTTCGGAGGTGTATTATCAGTTAAT‑‑GAGGG‑GG‑G‑AGTAGAATc < 1:2197496/86‑1 (MQ=255) gCAACACCTGGTTAACAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAAt > 2:2592578/1‑100 (MQ=255) gCAACACATGGTTAACAAGCATATGAA‑AATCCATAGAACTATTAATATACTGAGGTGGATTATCAGGTCCTG‑G‑GGG‑GG‑G‑AGTAGAATc < 1:2343892/88‑1 (MQ=255) cAACACCTGGTTAACAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAAtt > 2:961340/1‑100 (MQ=255) aaaCACCTGGTTAACAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTAGCAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGAGTGCCGAAtt > 1:193191/2‑100 (MQ=255) tGGTTAACAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTa > 2:232138/1‑100 (MQ=255) gTTAACACGAACATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑‑G‑GGGAGG‑G‑AGTAGGATCAGATTGCCGAATTAATACTAAg > 1:1361772/1‑100 (MQ=255) gTTAACAAGAATATGAA‑AAATCATAGCACTATTTATCCTCTGGGGTGATGTATCAGGTACT‑‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGa < 2:1111172/100‑1 (MQ=255) gTTAACAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGa > 2:190327/1‑100 (MQ=255) tAACAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGaa > 2:792678/1‑100 (MQ=255) tAACAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGaa > 2:2147279/1‑100 (MQ=255) aCAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAatt > 1:1651683/1‑100 (MQ=255) cAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAatta > 1:2489245/1‑100 (MQ=255) cAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAatta > 1:523213/1‑100 (MQ=255) cAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAatta > 1:2246516/1‑100 (MQ=255) aGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAattatt > 1:2435716/1‑100 (MQ=255) gAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGa > 2:691369/1‑100 (MQ=255) aTAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAAttt > 1:1036070/1‑100 (MQ=255) gCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGG‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTAc > 1:334982/1‑100 (MQ=255) gCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACaa > 2:268838/1‑100 (MQ=255) aCTATTAATCTACTGGGGGGTAGTATCAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAAc > 1:848842/1‑100 (MQ=255) cTATTAATCTACTGGGGGGTAGTATCAGGTACTGGG‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAAc > 1:1419078/1‑100 (MQ=255) ttAATCTACTGGGGGGTAGTATCAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACtctt > 1:643139/1‑100 (MQ=255) tAATCTACTGGGGGGTAGTATCAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACtcttc > 2:2216407/1‑100 (MQ=255) aaTCTACTGGGGGGTAGTATCAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACtcttct > 1:2473003/1‑100 (MQ=255) aCTGGGGGGTAGTATCAGGTACT‑‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCa > 2:2432488/1‑100 (MQ=255) aCTGGGGGGTAGTATCAGGTACT‑‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCa > 2:1768617/1‑100 (MQ=255) ggggggTAGTATCAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAg > 2:361438/1‑100 (MQ=255) ggggggTAGTATCAGGTACT‑‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAgg > 2:786312/1‑100 (MQ=255) gtgggTAGTATCAATTACT‑‑G‑GGG‑TG‑A‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAg < 1:312338/96‑1 (MQ=255) ggggTAGTATCAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGa > 2:1102213/1‑100 (MQ=255) ggTAGTGGCAGATAGT‑‑G‑GGGAGG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCttcttc < 1:769807/90‑1 (MQ=255) gtagtaTCAGGTACT‑‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAACTTACAACTCTTCTTCAGCAAGGAAACg > 1:1067689/1‑100 (MQ=255) agtaTCAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGc > 2:61017/1‑100 (MQ=255) gtaTCAGGTACT‑‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCt > 2:508555/1‑100 (MQ=255) cAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGt > 1:1478181/1‑100 (MQ=255) cAGGTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGt > 1:1156260/1‑100 (MQ=255) ggTACTG‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGttc > 2:797575/1‑100 (MQ=255) gTACT‑‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGttctt > 1:1111619/1‑100 (MQ=255) tACT‑‑G‑GGGTGG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGt < 1:46382/96‑1 (MQ=255) | TGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAA‑AAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACT‑‑G‑GGG‑GG‑G‑AGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGGAAACGCCTGGTTCTT > NC_000913/2185771‑2185963 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |