Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,754,784:1	(A)8→9	100%	intergenic (‑20/‑112)	ratA ← / → smpB	toxic UPF0083 family protein inhibitor of 70S ribosome formation/tmRNA‑binding trans‑translation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,754,776	1	.	A	56.3%	171.1 / 145.0	87	intergenic (‑12/‑120)	ratA/smpB	toxic UPF0083 family protein inhibitor of 70S ribosome formation/tmRNA‑binding trans‑translation protein
Reads supporting (aligned to +/- strand):  ref base . (16/22);  new base A (23/26);  total (39/48)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGA  >  NC_000913/2754679‑2754873                                                                                                    |                                                                                                  tGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑aa                                                                                                 >  2:1129898/1‑100 (MQ=255) tGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑aa                                                                                                 >  2:757568/1‑100 (MQ=255)     ccGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑aaaaaa                                                                                             >  2:1243277/1‑100 (MQ=255)     ccGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑aaaaaa                                                                                             >  1:153148/1‑100 (MQ=255)      cgcgCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑aaaaaaa                                                                                            <  1:1093025/100‑1 (MQ=255)        cgcTGTAGGGTACCAGTGCGGTCCGGCTACTCACAGGCAAAACTATTACCTTCAACAAAAATTCATCAAATAATATCATTTATCCTGCTG‑T‑a                                                                                                  <  1:1619017/92‑1 (MQ=255)         gcTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑aaaaaaaaac                                                                                         <  2:2542803/100‑3 (MQ=255)          cTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑aaaaaaaaacg                                                                                        <  2:1934406/100‑4 (MQ=255)           tGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑aaaaaaaaacgc                                                                                       <  2:932584/100‑5 (MQ=255)             tAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAACATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTa                                                                                      >  2:1385870/1‑100 (MQ=255)               gggTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATc                                                                                    >  1:820844/1‑100 (MQ=255)               gggTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATc                                                                                    >  2:1158376/1‑100 (MQ=255)               gggTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATc                                                                                    >  2:1278886/1‑100 (MQ=255)                ggTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATcc                                                                                   >  1:111120/1‑100 (MQ=255)                ggTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATcc                                                                                   >  1:935860/1‑100 (MQ=255)                 gTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATccc                                                                                  <  2:1233572/100‑1 (MQ=255)                    ccAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGgc                                                                               >  2:1649846/1‑100 (MQ=255)                       gTGCGGTCCGGCTAATCTGAGGCATAACAATTTCGATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACAATATCCCGgcgc                                                                             >  1:1166338/1‑99 (MQ=255)                        tGCGGTCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTgg                                                                          <  2:2210769/100‑1 (MQ=255)                         gCGGTCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTgg                                                                          >  2:2188865/1‑100 (MQ=255)                          cGGTCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTgg                                                                          >  2:1389814/1‑99 (MQ=255)                           ggTCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGt                                                                        >  2:304288/1‑100 (MQ=255)                           ggTCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGt                                                                        <  1:1045757/100‑1 (MQ=255)                           ggTCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTa                                                                       <  1:485136/100‑1 (MQ=255)                            gTCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTaa                                                                      >  1:2474596/1‑100 (MQ=255)                             tCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTaa                                                                      <  1:206241/100‑1 (MQ=255)                              ccGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAAc                                                                     <  2:1269344/100‑1 (MQ=255)                              ccGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACa                                                                    >  1:1343881/1‑100 (MQ=255)                              ccGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACa                                                                    <  2:1681911/100‑1 (MQ=255)                                ggCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATc                                                                  >  2:2206639/1‑100 (MQ=255)                                 gCTAATCTGAGGCATAACAATTTCCAACAACAAAAATCCAACAAATAATATCATTTATAAAAAAA‑A‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCg                                                                 <  1:1289777/100‑1 (MQ=255)                                   tAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCgg                                                                >  2:474054/1‑100 (MQ=255)                                     atgtaaGGCTTAACCATTTCCATCAAGAAAAATCTAACAAGTACTTTCGTTTATAAAAAAA‑AAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGt                                                              <  1:2453154/95‑1 (MQ=255)                                     aTCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACAt                                                                   >  1:1404890/1‑95 (MQ=255)                                      tCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGtt                                                             <  1:1974184/100‑1 (MQ=255)                                        tGAGGCATAACAATTTCCATCAACAAAAATCCTACAAATGATGTCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCAt                                                          <  1:1816185/100‑1 (MQ=255)                                        tGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCa                                                           >  2:1131317/1‑100 (MQ=255)                                        tGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCa                                                           <  2:128156/100‑1 (MQ=255)                                        tGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCggg                                                               >  2:2075067/1‑95 (MQ=255)                                        tGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCAt                                                          >  2:2105469/1‑100 (MQ=255)                                           ggCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGc                                                        >  1:873893/1‑100 (MQ=255)                                           ggCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCt                                                       <  2:28989/100‑1 (MQ=255)                                             cATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTTATGCt                                                       >  1:718642/1‑99 (MQ=255)                                             cATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTaa                                                     <  2:153149/100‑1 (MQ=255)                                             cATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTaa                                                     <  2:673254/100‑1 (MQ=255)                                              aTAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAg                                                    <  2:1539343/100‑1 (MQ=255)                                              aTAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAg                                                    <  2:1583786/100‑1 (MQ=255)                                               tAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAg                                                    <  2:1008319/100‑1 (MQ=255)                                               tAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAg                                                    >  2:1622940/1‑99 (MQ=255)                                               tAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGa                                                   <  1:1919981/100‑1 (MQ=255)                                               tAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCCGGGTAACATCGGGTTCATGCTAAg                                                    >  2:810843/1‑99 (MQ=255)                                               tAACAATTCCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAg                                                    <  1:2226392/100‑1 (MQ=255)                                                 aCAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGAt                                                  >  2:1275516/1‑100 (MQ=255)                                                 aCAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGAt                                                  >  1:1789685/1‑100 (MQ=255)                                                         cATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTg                                         >  1:739573/1‑100 (MQ=255)                                                         cATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTg                                         >  2:2513458/1‑100 (MQ=255)                                                          ataaacaaAAATACAACAAGTAATATCATTTATGCAAAAGAA‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTg                                         <  1:2241634/97‑1 (MQ=255)                                                           tCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGt                                        <  2:1184686/100‑1 (MQ=255)                                                            caacaaAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTcc                                      >  2:1282588/1‑100 (MQ=255)                                                             aacaaAAATCCAACAAGTAATACCATTAATCCTGCTG‑A‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTcc                                      <  1:1729599/99‑1 (MQ=255)                                                             aacaaAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTcc                                      <  2:151051/100‑1 (MQ=255)                                                              acaaAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTcccc                                    >  2:133514/1‑100 (MQ=255)                                                               caaAAATCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGTTAGAGCCTTGt                                        >  2:1727263/1‑96 (MQ=255)                                                               caaAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTccccc                                   >  1:2470186/1‑100 (MQ=255)                                                                aaaaaTCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGTGCCTTGTCCCCCg                                  <  1:1602294/100‑1 (MQ=255)                                                                 aaaaTCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCg                                  <  2:1292325/100‑1 (MQ=255)                                                                  aaaTCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTCTCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCg                                  <  2:2331719/99‑1 (MQ=255)                                                                  aaaTCCAACAAATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGc                                 <  2:1521992/100‑1 (MQ=255)                                                                  aaaTCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCa                                <  1:1501099/100‑1 (MQ=255)                                                                    gtCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAgg                              >  1:2014863/2‑100 (MQ=255)                                                                    aTCCAACAAATAAAATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAg                               <  1:565718/100‑1 (MQ=255)                                                                      ccAACAAATAATTCCATTTACCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAg                               <  2:397755/98‑1 (MQ=255)                                                                       caacaaATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGc                                 >  1:2077575/1‑95 (MQ=255)                                                                       caacaaATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGa                             >  1:1167695/1‑99 (MQ=255)                                                                       caacaaATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGAtt                           <  2:550538/100‑1 (MQ=255)                                                                       caacaaATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGAtt                           <  1:54440/100‑1 (MQ=255)                                                                         acaaATAATATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTg                          <  1:695918/100‑1 (MQ=255)                                                                            aataatATCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGata                       <  2:693746/100‑1 (MQ=255)                                                                            aataatATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGatat                      >  2:1878979/1‑100 (MQ=255)                                                                                atatCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATgggg                  <  2:1316938/100‑1 (MQ=255)                                                                                 tatCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATgggg                  <  1:84693/100‑1 (MQ=255)                                                                                 tatCATTTATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATgggg                  >  1:1927946/1‑100 (MQ=255)                                                                                  atCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGgtg                <  1:443392/100‑1 (MQ=255)                                                                                   tCAATTATCCGGCAG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGgt                 <  1:2117392/99‑1 (MQ=255)                                                                                    cATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGtt              >  2:1692659/1‑100 (MQ=255)                                                                                        tATCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTc           <  2:131467/100‑1 (MQ=255)                                                                                         aTCCTGCTG‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCg          <  2:1519500/100‑1 (MQ=255)                                                                                         aTCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGa         <  2:7350/100‑1 (MQ=255)                                                                                          tCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGAt        <  1:1824645/100‑1 (MQ=255)                                                                                            ctgctg‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGAt        <  1:802003/99‑1 (MQ=255)                                                                                             tgctg‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACCTCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGAttt      >  1:1381963/1‑100 (MQ=255)                                                                                             tgctg‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGAttt      <  1:1385862/100‑1 (MQ=255)                                                                                             tgctg‑TAAAAAAAAACGCTAACCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGAtt       <  1:1025231/99‑1 (MQ=255)                                                                                              gctg‑TAAAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTc     <  2:69812/100‑1 (MQ=255)                                                                                                tg‑T‑AAAAAAAACGCTATCCCGGCGCTTGGTAACATCGTGTTCATTCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGa  <  1:2045710/100‑1 (MQ=255)                                                                                                tg‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGa  >  2:322911/1‑100 (MQ=255)                                                                                                    |                                                                                                  TGCTCCGCGCTGTAGGGTACCAGTGCGGTCCGGCTAATCTGAGGCATAACAATTTCCATCAACAAAAATCCAACAAATAATATCATTTATCCTGCTG‑T‑AAAAAAAACGCTATCCCGGCGCTGGGTAACATCGGGTTCATGCTAAGATAGAGCCTTGTCCCCCGCAGGATTGATATGGGGTGTTTTCGATTTCAGA  >  NC_000913/2754679‑2754873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑