| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 705,972:1 | (T)9→10 | 100% | intergenic (+82/‑121) | nagE → / → glnS | N‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components/glutamyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 705,963 | 1 | . | T | 50.9% | 93.1 / 102.0 | 55 | intergenic (+73/‑130) | nagE/glnS | N‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components/glutamyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base . (15/12); new base T (16/12); total (31/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
CCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGC > NC_000913/705867‑706058 | ccGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑t‑tt > 1:715033/1‑100 (MQ=255)ccGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑t‑tt > 2:1489250/1‑100 (MQ=255) cGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑t‑ttt > 1:727129/1‑100 (MQ=255) cggTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑t‑ttttn < 2:1073466/98‑2 (MQ=255) cTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑t‑tttt‑t < 1:2398961/100‑1 (MQ=255) tGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑t‑tttt‑tt < 2:2069810/100‑1 (MQ=255) gTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑t‑tttt‑tt‑t > 1:2493693/1‑100 (MQ=255) tATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGCGATCTTCTCTGCCGC‑‑t‑tttt‑tt‑tt > 1:409056/1‑100 (MQ=255) tATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑t‑tttt‑tt‑t < 1:81708/99‑1 (MQ=255) aTGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑t‑tttt‑tt‑ttt > 2:318643/1‑99 (MQ=255) tGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑T‑TTTT‑TT‑Ttca < 1:147748/100‑1 (MQ=255) aaaTCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑t‑tttt‑tt‑tttcat > 2:469316/1‑96 (MQ=255) aaaTCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑T‑TTTT‑TT‑Ttcatc > 1:237389/1‑100 (MQ=255) aaaTCAAAAAGTAATCTGCTTTATGCCTGATGCAACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTAGAGAGCTTCTCTGCCGC‑‑T‑TTTT‑TT‑Ttcat > 1:386655/1‑99 (MQ=255) aaTCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑t‑tttt‑tt‑tttcatc < 2:2253890/100‑6 (MQ=255) aaTCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑T‑TTTT‑TT‑Ttcatc > 2:211323/1‑99 (MQ=255) aTCAAAAAGTAATCTGCTTTATGCCTGATGCGCCGCTTGAGCGTCGCATCCAACAATGACAAGCTGTAGAGATCGTCGCTGCCGC‑‑g‑tttt‑ta‑ttgtaaca > 2:2367225/1‑94 (MQ=255) aTCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCT‑T‑TTTT‑TT‑Ttcatca > 2:2283339/1‑100 (MQ=255) tCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑t‑tttt‑tt‑ttttcatca > 1:611623/1‑93 (MQ=255) cAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑t‑tttt‑tt‑tttttttttt > 1:2048165/1‑92 (MQ=255) aaaaaGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCT‑T‑TTTT‑TT‑TTCatcaatc > 2:1219505/1‑100 (MQ=255) aaaaaGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑t‑tttt‑tt‑tttttttttt > 1:554140/1‑91 (MQ=255) aaaaaGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑T‑TTTT‑TT‑TTCatcaatca > 2:1016066/1‑100 (MQ=255) aaaaGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCT‑T‑TTTT‑TT‑TTCatcaatca > 2:1894903/1‑100 (MQ=255) tCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑T‑TTTT‑TT‑TTCATCAATCATCCCCATaa > 2:1599396/1‑100 (MQ=255) tGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAAt > 2:1030450/1‑99 (MQ=255) gCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGTAGATCTTGGCGGCCGC‑‑C‑TTTT‑CT‑TTAATCAATAAAGCCCATAAtac > 1:2499312/1‑98 (MQ=255) gCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCT‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATc > 2:889194/1‑100 (MQ=255) cTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCT‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAAtgc > 2:1334134/1‑98 (MQ=255) ttATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTg < 1:236792/100‑1 (MQ=255) tGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCT‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGtt > 2:1562541/1‑100 (MQ=255) tGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCT‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGtt < 1:2272148/100‑1 (MQ=255) tGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTa > 1:2187976/1‑100 (MQ=255) ccTGATACGAAGCTAAAAAGTCGCATCCAACAATGCCAAGCGGTGGAGATCTTCTCTGCCGCT‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTg < 2:549875/96‑1 (MQ=255) gATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCT‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGAtt < 1:1025759/100‑1 (MQ=255) gATGCGACGCTCAAGCGTCGCATCCAACAATGACAAGCGGTGGGGATCTTCTCTGCCGCT‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGAtt > 2:909614/1‑100 (MQ=255) gCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCa > 2:437099/1‑100 (MQ=255) gCGACGCTCAAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCT‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTa > 2:991089/1‑98 (MQ=255) cGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCT‑T‑TTTT‑TT‑TTCATCAATCCTCCCCATAATCCTTGTTAGATTAtaa > 2:2342540/1‑98 (MQ=255) cGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCT‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATcc > 1:433312/1‑99 (MQ=255) cGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCaa > 2:140089/1‑100 (MQ=255) cGACGCTCAAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCT‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCa > 2:307610/1‑100 (MQ=255) aGCGTCGCATCCAAAAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTaaaaa < 2:1082666/100‑1 (MQ=255) cGTCGCATCCAACAATGACAAGCGGAGGAGATCTTCTCTGCCGCT‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTaaaaaa < 1:217437/100‑1 (MQ=255) cGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCT‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTa > 2:639311/1‑100 (MQ=255) agaaaacaaTGACAAGCGGTGGAGATCTTCTCTGCCGCT‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAAc < 1:414660/95‑1 (MQ=255) ccAACAATGAC‑AGCGGTGGAGATCTTCTCTGCCGC‑‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTg > 2:2432308/1‑100 (MQ=255) caacaaTGACAAGCGGTGGAGATCTTCTCTGCCGCT‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAg > 1:1117569/1‑98 (MQ=255) aacaaTGACAAGCGGTGGAGATCTTCTCTGCCGCT‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTg < 1:473734/100‑1 (MQ=255) caaTGACAAGCGGTGGAGATCTTCTCTGCCGCT‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTc > 2:2454854/1‑100 (MQ=255) caaTGACAAGCGGTGGAGAACTTCTCTGCCGCT‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTc < 2:282892/100‑1 (MQ=255) aaTGACAAGCGGTGGAGATCTTGTCTGCCGC‑‑TGTTTT‑TT‑TACAAAAAAAATCCCCATAATCCTTGTTAGATTCTCAATTTTAAAAAACTAACa > 1:1735090/1‑93 (MQ=255) aaTGACAAGCGGTGGAGATCTTCTCTGCCGCT‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTc < 1:154407/99‑1 (MQ=255) aTGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCa > 1:35378/1‑98 (MQ=255) gACTAGCGGTGGAGAGATTCTCTGCCTCT‑T‑TTTT‑TT‑TTCATCAATCATCACCATAATCCTTGTTAGATTATCAATTTTAAGAAACTAACAGTTGTCAGcc < 1:1176249/100‑1 (MQ=255) gTGGAGATCTTCTCTGCCGC‑‑T‑TTTTATT‑TTCATCAATCAACCCCATCATCCTTGTTAGATTAACAAAAAAAAAAAACTAACAGTTGTCAGCCTGTCCCGc < 1:1265035/100‑1 (MQ=255) gTGGAGATCTTCTCTGCCGC‑‑T‑CTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCt < 1:2540739/100‑1 (MQ=255) agaTCTTCTCTGCCGC‑‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTtata < 1:2589480/100‑1 (MQ=255) gaTCTTCTCTGCCGCTTT‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTtat > 2:475291/1‑100 (MQ=255) gaTCTTCTCTGCCGC‑‑TATTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTta < 2:8077/98‑1 (MQ=255) cttctCTGCAGCT‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAg < 2:609969/99‑1 (MQ=255) ttctCTGCCGC‑‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATc < 1:1016063/100‑1 (MQ=255) ctctGCCGCT‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCa > 2:2367729/1‑100 (MQ=255) ctctGCCGC‑‑T‑TTTT‑TTATTAATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGAt < 2:2330451/98‑1 (MQ=255) ctccGCCGC‑‑TATTAT‑TT‑TTCATCAATCATCCCCATAAGCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCa < 2:1518495/100‑1 (MQ=255) ctGCCGC‑‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATAc > 1:2567566/1‑100 (MQ=255) ctGCCGC‑‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATAc < 2:727132/100‑1 (MQ=255) ctGCCGC‑‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATAc < 2:1423052/100‑1 (MQ=255) tGCCGCT‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATAc < 2:1344449/100‑1 (MQ=255) gCCGCT‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATaa < 1:450613/91‑1 (MQ=255) gCCGC‑‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGc > 2:1863599/1‑100 (MQ=255) | CCGCTGTATGAAATCAAAAAGTAATCTGCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGC‑‑T‑TTTT‑TT‑TTCATCAATCATCCCCATAATCCTTGTTAGATTATCAATTTTAAAAAACTAACAGTTGTCAGCCTGTCCCGCTTATAAGATCATACGC > NC_000913/705867‑706058 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |