| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,197,853 | T→C | 100% | N129D (AAT→GAT) | ymfE ← | e14 prophage; putative inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,197,853 | 0 | T | C | 96.4% | 265.0 / ‑1.0 | 83 | N129D (AAT→GAT) | ymfE | e14 prophage; putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (1/2); new base C (36/44); total (37/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.22e-01 | |||||||||||
GAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATA > NC_000913/1197756‑1197949 | gAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGc < 1:1087164/100‑1 (MQ=255)gAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGc > 1:296400/1‑100 (MQ=255)gAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGc > 1:297097/1‑100 (MQ=255) aaaTTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCt > 1:1849637/1‑99 (MQ=255) aaaTTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTc < 2:11960/100‑1 (MQ=255) aaTTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCa < 1:1789935/100‑1 (MQ=255) aaTTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCa < 2:544162/100‑1 (MQ=255) ttCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCAtt > 1:1323199/1‑100 (MQ=255) ttCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCAtt < 1:445622/100‑1 (MQ=255) ttCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCAtt < 1:2001/100‑1 (MQ=255) cAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTAc > 2:2498064/1‑100 (MQ=255) aaaaaTATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACAt > 1:2519220/1‑100 (MQ=255) aaTATCCAAATATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGTGTAATATAGATCGCGCATTACATtgt < 1:930386/100‑1 (MQ=255) atatCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATtgtg > 2:331184/1‑100 (MQ=255) tatCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGa < 1:679665/100‑1 (MQ=255) tCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGc < 2:1323206/100‑1 (MQ=255) tcAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCt < 1:1200360/99‑1 (MQ=255) cAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCtt > 2:1556246/1‑100 (MQ=255) cAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCtt > 2:464682/1‑100 (MQ=255) cAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCta > 2:1200364/1‑99 (MQ=255) ataCTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCtttatt < 2:2410844/100‑1 (MQ=255) taCTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCtttattt > 2:1939893/1‑100 (MQ=255) taCTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCtttattt < 2:479832/100‑1 (MQ=255) aCTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCtttattta > 2:774003/1‑100 (MQ=255) cTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCtttattta > 2:1290001/1‑99 (MQ=255) cTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTTTTTaa > 1:118616/1‑100 (MQ=255) tttATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATATATCGCTCATTACATTGTGAGCTTCATCTTTATTTaa < 1:2224599/99‑1 (MQ=255) ctttCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAAttt < 2:1958110/98‑1 (MQ=255) ttCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAAtttag > 1:1958100/1‑98 (MQ=255) ttCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTc < 2:2325086/100‑1 (MQ=255) tCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCt > 2:1031092/1‑100 (MQ=255) ccAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTg > 2:1154280/1‑100 (MQ=255) ccAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTg < 2:792688/100‑1 (MQ=255) cAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGt < 2:2299610/100‑1 (MQ=255) agaagaGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGtt < 2:1976703/100‑1 (MQ=255) gaagaGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTg > 2:542596/1‑100 (MQ=255) gaagaGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTg < 2:1610787/100‑1 (MQ=255) gaagaGTTCAATAGAATGTTTGTCTTCAATTTTTCTTACTTCATGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTg < 1:943691/100‑1 (MQ=255) gagTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACt < 2:1849647/100‑1 (MQ=255) gagTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACt < 2:1429434/100‑1 (MQ=255) gagTTCAATATAATGTTTGTCTTCAATTTTTCTTAATTAAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGagt > 1:1164502/1‑98 (MQ=255) gTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTCATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTc > 1:30414/1‑99 (MQ=255) gTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTcc < 1:1275203/100‑1 (MQ=255) gTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTcc < 2:557479/100‑1 (MQ=255) gTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTcc > 1:134546/1‑100 (MQ=255) ttCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCa < 1:2498045/100‑1 (MQ=255) cAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGc > 2:2175577/1‑100 (MQ=255) aaTGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCg < 1:2003293/100‑1 (MQ=255) ttGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATa < 2:2520650/100‑1 (MQ=255) gTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAAc < 2:2418049/100‑1 (MQ=255) gTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAAc > 2:1023966/1‑100 (MQ=255) ttCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGt < 2:1046134/100‑1 (MQ=255) ttCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGt > 2:2451480/1‑100 (MQ=255) ttCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGt > 2:514467/1‑100 (MQ=255) tCAATTTTTTTTTTTTTTTGGTAATATAAATCGCTCATTACATTGTGAGCTTCATCTGTATTTAATTTTCTGTTGACTCCAGCTCTCCGTTATATCTGtt > 1:1011830/1‑100 (MQ=255) tCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCCTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGtt > 2:1909667/1‑100 (MQ=255) cAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGttt < 2:2219392/100‑1 (MQ=255) aTTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTta < 2:175445/100‑1 (MQ=255) aTTTTTCTTACTTCAGGGTAATATAGATCGCTCATTAAATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCAACGTGATAACGGTTTta < 1:1509582/100‑1 (MQ=255) tttttCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTtat < 1:1664668/100‑1 (MQ=255) tCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAAtt < 1:1871294/100‑1 (MQ=255) cTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTa < 1:1392265/100‑1 (MQ=255) cTTACCTCAGTGTATCATCGATCGCTAAATACATTGTGACATACATCTTTATTTAATTTACTGCTGACTCGCGATCAAAGTGATAACGGTTTTATGAtt < 1:859698/99‑1 (MQ=255) tACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGa > 2:2034166/1‑100 (MQ=255) tACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGa > 2:1783717/1‑100 (MQ=255) aCTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGAt < 2:988096/100‑1 (MQ=255) cTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATg > 2:2066896/1‑100 (MQ=255) ttCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGc < 1:1852142/100‑1 (MQ=255) gggTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTAt < 2:595661/100‑1 (MQ=255) gTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATcc < 1:1956959/100‑1 (MQ=255) gTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATcc < 2:1516558/100‑1 (MQ=255) gTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATcc < 2:1616029/100‑1 (MQ=255) tAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATccc > 2:1240208/1‑100 (MQ=255) tAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATccc < 1:2431103/100‑1 (MQ=255) atatAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCaa > 2:2435707/1‑100 (MQ=255) atatAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCaa > 2:2463522/1‑100 (MQ=255) ataGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAg < 1:1307002/100‑1 (MQ=255) ataGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAg < 2:1680176/100‑1 (MQ=255) taGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAg > 2:1982672/1‑99 (MQ=255) aGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTGTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGat < 1:882038/100‑1 (MQ=255) gATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGata > 1:839327/1‑100 (MQ=255) gATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGaag > 1:2388674/1‑98 (MQ=255) gATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGCGATAACGGTTTTATAATTAGATGCTTATCCCAAAGata > 1:1411708/1‑100 (MQ=255) | GAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATA > NC_000913/1197756‑1197949 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |