| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,270,726 | C→G | 15.6% | intergenic (‑381/+23) | ldrC ← / ← chaA | toxic polypeptide, small/calcium/sodium:proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,270,726 | 0 | C | G | 15.6% | 217.2 / 31.0 | 83 | intergenic (‑381/+23) | ldrC/chaA | toxic polypeptide, small/calcium/sodium:proton antiporter |
| Reads supporting (aligned to +/- strand): ref base C (35/34); new base G (8/5); total (44/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01 | |||||||||||
TGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCG > NC_000913/1270627‑1270824 | tGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGCCCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTAcc < 1:2324038/100‑1 (MQ=255)tGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTAcc > 2:1066700/1‑100 (MQ=255) gCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCg > 2:1128791/1‑100 (MQ=255) gggTGTGGTACAGGCTCGCAGTTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCa > 1:1584054/1‑100 (MQ=255) gtgtgtTACAGGCTCGCAATTCTGATTAACGACCTGTCCATGTATTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATc < 1:2142293/100‑1 (MQ=255) gtgtGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATc > 1:507357/1‑100 (MQ=255) tgtgGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCg < 2:245835/100‑1 (MQ=255) ggTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCggag < 2:1350321/100‑1 (MQ=255) ggTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCggag > 1:1310115/1‑100 (MQ=255) gTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCTCCGATGACGGTAATCggggg < 2:37344/100‑1 (MQ=38) gTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCggagg < 2:2399161/100‑1 (MQ=255) gTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCggagg < 2:1829292/100‑1 (MQ=255) gTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCggag < 2:154613/99‑1 (MQ=255) aCAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCTCCGATGACGGTAATCGggggag < 2:870358/100‑1 (MQ=38) cAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGgaga > 2:1720638/1‑100 (MQ=255) cAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGgaga > 1:499116/1‑100 (MQ=255) aGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCTCCGATGACGGTAATCGGGGGAGat < 1:2406517/100‑1 (MQ=37) ggCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGata < 1:151364/100‑1 (MQ=255) ggCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGata < 2:1925593/100‑1 (MQ=255) cgcGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGAtata < 1:1098481/98‑1 (MQ=255) tCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATaa > 1:1758368/1‑100 (MQ=255) cGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCTCCGATGACGGTAATCGGGGGAGataatat < 1:670529/100‑5 (MQ=25) cGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCTCCGATGACGGTAATCGGGGGAGataatat < 2:812562/100‑5 (MQ=25) cGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAAc > 2:1198550/1‑100 (MQ=255) cGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAAc > 2:1152601/1‑100 (MQ=255) ttCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCa < 1:658671/100‑1 (MQ=255) cTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCAGATTACGGTCATCGGAGAAGATATAACAGCCAgg > 1:1193754/1‑100 (MQ=255) tGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTc > 1:2081256/1‑96 (MQ=255) gATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCa > 1:2185615/1‑100 (MQ=255) aTTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCaa < 2:786800/100‑1 (MQ=255) ttAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCaaa < 1:1066696/100‑1 (MQ=255) ttAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCaaa < 1:781804/100‑1 (MQ=255) ttAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCaaa > 1:1311534/1‑100 (MQ=255) tAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCa < 1:1517237/97‑1 (MQ=255) tAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAat > 1:2095265/1‑100 (MQ=255) aaCGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAata > 2:216606/1‑100 (MQ=255) aaCGACCGGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAata < 2:1508765/100‑1 (MQ=255) aCGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAatat > 2:744673/1‑100 (MQ=255) gACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCg < 2:1542315/100‑1 (MQ=255) cccTGTCCAGGTAGTATTAAGAAATACAATAAGCAGGAAGTTATATTATCTCCCCCGATTACCGTCATCGGAGGACATATAACTGTCAGGCAAATATCGt < 1:2312035/99‑1 (MQ=255) ccTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGtc < 2:1165569/100‑1 (MQ=255) cTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGtca < 1:634867/100‑1 (MQ=255) tGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGcca > 1:2010520/1‑99 (MQ=255) ccAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCaa > 1:1291560/1‑100 (MQ=255) aGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAAt > 1:1487186/1‑100 (MQ=255) tagtaTGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAgg > 1:737004/1‑100 (MQ=255) agtaTGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAggc < 1:2095816/100‑1 (MQ=255) taTGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAggcgg > 1:1524314/1‑100 (MQ=255) aTGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAggcggc < 1:744670/100‑1 (MQ=255) tGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCa > 2:2318164/1‑100 (MQ=255) tGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCa > 2:1421680/1‑100 (MQ=255) gaaATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACAGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACa > 1:1669882/1‑100 (MQ=255) aaTACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGt > 1:2183671/1‑100 (MQ=255) aTACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTg < 2:2068019/100‑1 (MQ=255) cAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGAtgcgc > 2:734214/1‑100 (MQ=255) aaTTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGAtgcgctgcg < 2:90522/100‑1 (MQ=255) aTTTATATTATCTCCCCCGATTAGCGTCATCGGAGGAGATATAACTGTCAGGCAAATAGCGTCATCAGAGATGCGGCAAACAGTGCCAGAttcgcggc > 1:116584/1‑98 (MQ=255) aTTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGAtgcgctgcgc > 2:919150/1‑100 (MQ=255) ttATATTATCTCCCCCGATTACCGTCATCGGAGGGGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGAtgcgctgcgc < 1:2543070/98‑1 (MQ=255) tataTTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCAAt > 1:270105/1‑100 (MQ=255) gttatatCTCCTCCGATGACGGTAATCGGGGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCAtt > 2:760811/5‑100 (MQ=25) atatTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCAtt < 2:1835472/100‑1 (MQ=255) ttatatCTCCTCCGATGACGGTAATCGGGGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTg > 1:114890/4‑100 (MQ=25) tattatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTg > 2:410063/1‑100 (MQ=255) tattatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTg < 1:2173530/100‑1 (MQ=255) tattatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTg < 1:1150274/100‑1 (MQ=255) tattatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTg < 2:1055679/100‑1 (MQ=255) tattatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTg < 1:1877403/100‑1 (MQ=255) tattatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTg < 2:1918009/100‑1 (MQ=255) tattatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTg > 1:2428176/1‑100 (MQ=255) ttatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAg < 1:1307468/100‑1 (MQ=255) tatCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGc < 1:73863/100‑1 (MQ=255) atCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCa > 2:1942210/1‑100 (MQ=255) tctcCTCCGATGACGGTAATCGGGGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCAc > 1:97158/1‑100 (MQ=37) tctcCTCCGATGACGGTAATCGGGGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCAc > 2:377004/1‑100 (MQ=37) tctcCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGACAAACAGTGCCAGATGCGCTGCGCCATTGAGCa < 2:431265/99‑1 (MQ=255) ctcctcCGATGACGGTAATCGGGGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACg > 2:145139/1‑100 (MQ=37) ctcctcCGATGACGGTAATCGGGGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACg > 2:317909/1‑100 (MQ=37) tcctccGATGACGGTAATCGGGGGAGAGATAACTGTCAGGCAAATATCGTCATCAAATAGGAGGCCAACAGTGCCAGATGCGCTGCGCCATTGAGCACGt > 1:123182/1‑100 (MQ=37) cccccGATTACCGTCATCGGAGGAGATATAACTGTCCGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGtt > 1:2365023/1‑100 (MQ=255) cccGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAg > 2:2556673/1‑100 (MQ=255) cccGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAg < 1:216604/100‑1 (MQ=255) cccGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAg > 2:1736139/1‑100 (MQ=255) cGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa > 2:289434/1‑100 (MQ=255) cGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa > 2:288925/1‑100 (MQ=255) ccGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCg < 2:1993211/100‑1 (MQ=255) ccGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCg > 2:914442/1‑100 (MQ=255) | TGCAGGGTGTGGTACAGGCTCGCAATTCTGATTAACGACCTGTCCAGGTAGTATGAAGAAATACAATAAGCAGGAATTTATATTATCTCCCCCGATTACCGTCATCGGAGGAGATATAACTGTCAGGCAAATATCGTCATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCG > NC_000913/1270627‑1270824 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |