Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,642,370 C→G 18.8% intergenic (+303/+119) cspF → / ← quuQ Qin prophage; cold shock protein/Qin prophage; putative antitermination protein Q

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,642,3700CG18.8% 245.0 / 51.3 101intergenic (+303/+119)cspF/quuQQin prophage; cold shock protein/Qin prophage; putative antitermination protein Q
Reads supporting (aligned to +/- strand):  ref base C (49/33);  new base G (6/13);  total (55/46)
Fisher's exact test for biased strand distribution p-value = 3.96e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.05e-01

Only 100 of 107 total aligned reads displayed.

CAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGC  >  NC_000913/1642271‑1642469
                                                                                                   |                                                                                                   
cAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCtc                                                                                                     >  2:950953/1‑100 (MQ=255)
cAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCtc                                                                                                     >  1:61559/1‑100 (MQ=255)
cAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCtc                                                                                                     >  2:1458939/1‑100 (MQ=255)
  aaTAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCtcat                                                                                                   >  2:22009/1‑100 (MQ=255)
     aaCCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAg                                                                                                >  1:2467299/1‑100 (MQ=255)
     aaCCAAGACACGAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCCTTGCCCCATTTAAAGCACAAAACCCGCTCATCAg                                                                                                >  1:1358899/1‑100 (MQ=255)
      aCCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGc                                                                                               >  2:644869/1‑100 (MQ=255)
      aCCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGc                                                                                               >  2:1539610/1‑100 (MQ=255)
      aCCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGc                                                                                               >  2:2395034/1‑100 (MQ=255)
       ccAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCg                                                                                              >  1:1338028/1‑100 (MQ=255)
        cAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCgg                                                                                             >  1:313177/1‑100 (MQ=255)
            tCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGTAGAAAACCCGCTGATGAGCGGGttt                                                                                         <  2:2411314/100‑1 (MQ=38)
             cACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGtttt                                                                                        >  2:244029/1‑100 (MQ=255)
              aCCAGCTAAATAATAAGTTAACAGACATGATTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAATCCCGCTGATGAGCGGGttttg                                                                                       <  2:2513645/100‑2 (MQ=255)
              aCCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGCTGAGAATCAACATTACCAATGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTc                                                                                       >  1:2311664/1‑100 (MQ=255)
              aCCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCTATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTc                                                                                       <  1:543063/100‑1 (MQ=255)
              aCCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTGATGAGCGGGttttg                                                                                       <  2:2514081/100‑2 (MQ=255)
              aCCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTGATGAGCGGGttttg                                                                                       <  2:1123156/100‑2 (MQ=255)
              aCCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTGATGAGCGGGttttg                                                                                       <  2:834067/100‑2 (MQ=255)
              aCCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTGATGAGCGGGttttg                                                                                       <  2:407563/100‑2 (MQ=255)
              aCCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTc                                                                                       <  2:588829/100‑1 (MQ=255)
               ccAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTGATGAGCGGGttttgt                                                                                      <  1:2060495/100‑3 (MQ=255)
               ccAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTGATGAGCGGGttttgt                                                                                      <  2:264860/100‑3 (MQ=255)
               ccAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTGATGAGCGGGttttgt                                                                                      <  1:2518699/100‑3 (MQ=255)
                cAGCTAAATAATAATTTAACAGACATGAGTCCAGGCATGAGATTCAACATTACCATTGCCCCATTTAAAGCACCAAACCCGCTGATGAGCGGGttttgt                                                                                      <  1:2568075/99‑3 (MQ=38)
                cAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTGATGAGCGGGttttgtg                                                                                     <  1:2050569/100‑4 (MQ=39)
                cAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTGATGAGCGGGttttgtg                                                                                     <  2:1828143/100‑4 (MQ=39)
                  gCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACt                                                                                   >  2:2083136/1‑100 (MQ=255)
                  gCTAAATAATAAGTTAACAGACATGAGTCCAGGAATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGAGTTTTCTACt                                                                                   >  1:1703344/1‑100 (MQ=255)
                   cTAAATATTAAGTTAACAGTCATGTTTCCCGTGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTGATGAGCGGGTTTTGTGCtt                                                                                  <  2:860890/100‑1 (MQ=25)
                   cTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACtt                                                                                  >  1:2229597/1‑100 (MQ=255)
                      aataatAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACttttt                                                                               <  2:639764/100‑1 (MQ=255)
                       ataataAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTc                                                                              >  1:2580356/1‑100 (MQ=255)
                        taataaGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAACCCCGCTCATCAGCGGGTTTTCTACTTTTTCt                                                                             >  2:2426559/1‑100 (MQ=255)
                        taataaGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCt                                                                             <  2:1321512/100‑1 (MQ=255)
                        taataaGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCt                                                                             <  2:1770333/100‑1 (MQ=255)
                        taataaGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCt                                                                             >  1:2053913/1‑100 (MQ=255)
                          ataaGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCACTGCCCCATTTAAAGCACAATACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTa                                                                           <  1:1542872/100‑1 (MQ=255)
                             aGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACg                                                                        >  1:862845/1‑100 (MQ=255)
                                 aaCAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCgg                                                                    >  1:747509/1‑100 (MQ=255)
                                   cAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCggg                                                                   <  1:518134/99‑1 (MQ=255)
                                    aGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGta                                                                 <  1:1239495/100‑1 (MQ=255)
                                     gACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGtat                                                                <  2:1064595/100‑1 (MQ=255)
                                     gACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTATTTCTTAACGTCGGGtat                                                                >  1:1052189/1‑100 (MQ=255)
                                      aCATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGtata                                                               <  1:1953756/100‑1 (MQ=255)
                                        aTGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACa                                                             <  1:1593809/100‑1 (MQ=255)
                                         tGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCgggagatcgg                                                            >  2:572144/1‑93 (MQ=255)
                                         tGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACaa                                                            >  2:749823/1‑100 (MQ=255)
                                         tGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACaa                                                            >  2:1714768/1‑100 (MQ=255)
                                            gTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAg                                                          <  2:1988061/99‑1 (MQ=255)
                                            gTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGc                                                         >  1:1156152/1‑100 (MQ=255)
                                               ccGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCa                                                      >  2:114880/1‑100 (MQ=255)
                                                  ggATGAGATTCAAGATTACCATTGCCCCCTTTAGAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGcaca                                                      >  1:1873669/1‑97 (MQ=255)
                                                  ggATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCg                                                   >  1:1214031/1‑100 (MQ=255)
                                                     tGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGtt                                                 >  2:1335959/1‑99 (MQ=255)
                                                     tGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTg                                                <  2:2398269/100‑1 (MQ=255)
                                                     tGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTg                                                <  2:2398407/100‑1 (MQ=255)
                                                       agaTTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGaa                                              <  1:416813/100‑1 (MQ=255)
                                                       agaTTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGaa                                              >  1:678529/1‑100 (MQ=255)
                                                        gaTTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGaca                                             >  1:1569033/1‑98 (MQ=255)
                                                         aTTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGaaaa                                            <  1:157574/100‑1 (MQ=255)
                                                         aTTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGaaaa                                            <  2:1951811/100‑1 (MQ=255)
                                                            cAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAAt                                         >  1:2012040/1‑100 (MQ=255)
                                                             aaCATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTCACGTCGGGTATACAAAGCCCATCGTTGaaaaaa                                          >  1:2073938/1‑98 (MQ=255)
                                                               cATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAAtttt                                      <  1:1458898/100‑1 (MQ=255)
                                                                   aCCATTTCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACACAGCCCATCGTTGAAAAAATTTTATc                                   <  1:476562/99‑1 (MQ=255)
                                                                   aCCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATcc                                  <  2:313179/100‑1 (MQ=255)
                                                                   aCCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATcc                                  >  2:313916/1‑100 (MQ=255)
                                                                      aTTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGTTTATACAAAGCCCATCGTTAAAAAAATTTTATCCat                                <  1:232331/99‑1 (MQ=255)
                                                                         gCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATAttt                            >  1:372610/1‑100 (MQ=255)
                                                                          ccccATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATAtttt                           >  2:901397/1‑100 (MQ=255)
                                                                          ccccATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGCTATACAAAGCCCATCGTTGAAAAAATTTTATCCATAtttt                           >  1:98212/1‑100 (MQ=255)
                                                                           cccATTTAAAGCACAAAACCCGCTCATCAGCTGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCATCGTTGAAAAAATTTTATCCATAtttttt                         <  1:670649/100‑1 (MQ=255)
                                                                           cccATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTTTTTTTTTTTTTTATACAAAGCCCCTCGTTGAAAAAATTTTATCCATAttttt                          >  1:156052/1‑100 (MQ=255)
                                                                           cccATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATAttttt                          >  1:291214/1‑100 (MQ=255)
                                                                            ccATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATAtttttt                         <  2:837782/100‑1 (MQ=255)
                                                                             cgatctaaGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTg                        <  1:1942406/94‑1 (MQ=255)
                                                                             cATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTg                        <  2:1405789/100‑1 (MQ=255)
                                                                             cATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGCGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTg                        <  1:72922/100‑1 (MQ=255)
                                                                                 tAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGaaaa                    <  2:2245082/100‑1 (MQ=255)
                                                                                 tAAAACACAAAACCCGCTCATCAGCGGGTTTACTACTTTTTCTTAACGGCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATACAAATGTTTTGaaa                     >  1:841913/1‑99 (MQ=255)
                                                                                  aaaGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGCAAAAATTTTATCCATATTTTTTGaaaaa                   >  1:1348106/1‑100 (MQ=255)
                                                                                   aagtagaaaaCCCGCTGATGAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAAt                  >  2:2195947/7‑100 (MQ=38)
                                                                                   aaGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGagatcg                  >  2:1942416/1‑95 (MQ=255)
                                                                                    aGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATg                 >  2:44194/1‑100 (MQ=255)
                                                                                    aGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATg                 >  2:45875/1‑100 (MQ=255)
                                                                                     gCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGACAAAATTTTATCCATATTTTTTGAAAAATGc                >  1:2505316/1‑100 (MQ=255)
                                                                                      cacaAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCa               <  2:308927/100‑1 (MQ=255)
                                                                                      cacaAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCa               <  2:1972128/100‑1 (MQ=255)
                                                                                       agaaaaCCCGCTGATGAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCaa              >  2:631970/3‑100 (MQ=255)
                                                                                        caAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAACTTTTATCCATATTTTTTGAAAAATGCaaa             >  1:620430/1‑100 (MQ=255)
                                                                                         aaaaCCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAc            <  1:20979/100‑1 (MQ=255)
                                                                                          aaaCCCGCTGATGAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAATAAATTTTATCCATATTTTTTGAAAAATGCAAAca           >  1:1363354/1‑100 (MQ=255)
                                                                                          aaaCCCGCTGATGAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAca           >  1:1630460/1‑100 (MQ=255)
                                                                                          aaaCCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAca           >  2:1371995/1‑100 (MQ=255)
                                                                                            aCCCGCTGATGAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAcatc         >  1:2407928/1‑100 (MQ=255)
                                                                                             nccGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAcatca        >  1:283758/2‑100 (MQ=255)
                                                                                               cGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATg      >  1:1392954/1‑100 (MQ=255)
                                                                                               cGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATg      <  2:2330228/100‑1 (MQ=255)
                                                                                                   catcaGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGc  >  2:2103544/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
CAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGC  >  NC_000913/1642271‑1642469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: