Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,657,901 A→T 24.5% intergenic (+31/‑168) ynfD → / → ynfE DUF1161 family periplasmic protein/putative selenate reductase, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,657,9010AT24.5% 143.1 / 53.5 86intergenic (+31/‑168)ynfD/ynfEDUF1161 family periplasmic protein/putative selenate reductase, periplasmic
Reads supporting (aligned to +/- strand):  ref base A (38/27);  new base T (10/11);  total (48/38)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.08e-02

CCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCC  >  NC_000913/1657803‑1657998
                                                                                                  |                                                                                                 
ccaccaGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACaaa                                                                                                  <  1:1981839/100‑1 (MQ=255)
   ccaGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGCAACACACTATCGACTGAACGCCGGATATGACAAATcc                                                                                               >  1:2194944/1‑100 (MQ=255)
    caGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCg                                                                                              <  1:1016038/100‑1 (MQ=255)
      gCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGTTGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGc                                                                                            <  1:1127528/100‑1 (MQ=255)
      gCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATTTGTCATATCCGGc                                                                                            <  1:105191/100‑1 (MQ=255)
      gCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATTTGTCATATCCGGc                                                                                            <  1:922805/100‑1 (MQ=255)
      gCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATTTGTCATATCCGGc                                                                                            <  2:2561282/100‑1 (MQ=255)
      gCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGc                                                                                            >  2:1094316/1‑100 (MQ=255)
      gCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGc                                                                                            <  1:2417611/100‑1 (MQ=255)
       cGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATTTGTCATATCCGGCg                                                                                           <  2:2074210/100‑1 (MQ=255)
       cGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATTTGTCATATCCGGCg                                                                                           <  2:2105591/100‑1 (MQ=255)
        ggTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATTTGTCATATCCGGCgt                                                                                          <  1:1449607/100‑2 (MQ=255)
        ggTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATTTGTCATATCCGGCgt                                                                                          <  1:1751393/100‑2 (MQ=255)
        ggTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATTTGTCATATCCGGCgt                                                                                          <  1:903687/100‑2 (MQ=255)
             cGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTACCACATTATCGACTGAACGCCGGATTTGTCATATCCGGCgttcagt                                                                                     <  2:1073641/100‑7 (MQ=37)
             cGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATTTGTCATATCCGGCgttcagt                                                                                     <  2:1761173/100‑7 (MQ=38)
              gTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATTTGTCATATCCGGCgttcagtc                                                                                    <  2:1045060/100‑8 (MQ=37)
              gTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGaa                                                                                    >  2:749899/1‑100 (MQ=255)
               tctcTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAAc                                                                                   <  1:895122/100‑1 (MQ=255)
               tcgctGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATCATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAAc                                                                                   <  1:1986014/97‑1 (MQ=255)
                  ctGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATa                                                                                >  1:921602/1‑100 (MQ=255)
                  ctGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATa                                                                                <  2:2495847/100‑1 (MQ=255)
                    gCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATaca                                                                              <  1:1932608/100‑1 (MQ=255)
                    gCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATaca                                                                              <  2:324441/100‑1 (MQ=255)
                    gCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATaca                                                                              >  2:769809/1‑100 (MQ=255)
                     cTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATacaa                                                                             >  2:136885/1‑100 (MQ=255)
                     cTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATacaa                                                                             >  2:1545490/1‑100 (MQ=255)
                        ccGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACAt                                                                          >  2:183234/1‑100 (MQ=255)
                               gTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCa                                                                   <  2:1094029/100‑1 (MQ=255)
                               gTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCa                                                                   >  2:367666/1‑100 (MQ=255)
                               gTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCa                                                                   >  2:770858/1‑100 (MQ=255)
                                tCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATCATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCAc                                                                  <  1:626069/100‑1 (MQ=255)
                                    gCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTCAACATACAACATAATACCACCtta                                                              >  1:207042/1‑100 (MQ=255)
                                      cAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCttatt                                                            >  1:347402/1‑100 (MQ=255)
                                      cAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCttatt                                                            >  2:600472/1‑100 (MQ=255)
                                      cAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCAACCttagt                                                            >  1:1566596/1‑98 (MQ=255)
                                        ggATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTAc                                                          <  2:1850711/100‑1 (MQ=255)
                                         gATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACt                                                         <  1:1545481/100‑1 (MQ=255)
                                             gcgcgcCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCAt                                                      <  1:656282/99‑1 (MQ=255)
                                               gcgcCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATAcc                                                   <  1:600469/100‑1 (MQ=255)
                                                 gcCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCt                                                 <  1:2347601/100‑1 (MQ=255)
                                                  ccTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCtt                                                >  2:185564/1‑100 (MQ=255)
                                                  ccTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCtt                                                >  1:2158787/1‑100 (MQ=255)
                                                     gCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACCAATCCGGCGATTTCAACATACAACATAATCCCACCTTATTACTCATACCCTTCTa                                             >  1:611210/1‑100 (MQ=255)
                                                       cGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTAtt                                           >  2:94994/1‑100 (MQ=255)
                                                       cGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTAtt                                           <  2:704208/100‑1 (MQ=255)
                                                       cGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTAtt                                           >  1:1911811/1‑100 (MQ=255)
                                                         aaCCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGa                                         <  2:1112100/100‑1 (MQ=255)
                                                          aCCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGat                                        <  1:1888856/100‑1 (MQ=255)
                                                             gCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATg                                     <  1:1932318/100‑1 (MQ=255)
                                                                gTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACCCATACCCTTCTATTGATATGGAt                                  >  2:1225347/1‑100 (MQ=255)
                                                                   acacATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATtaa                               >  1:703048/1‑100 (MQ=255)
                                                                    cacaTTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATtaat                              >  2:1971384/1‑100 (MQ=255)
                                                                    cacaTTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATtaat                              <  2:2187283/100‑1 (MQ=255)
                                                                     tccgatctgACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATtaata                             <  1:582861/92‑1 (MQ=255)
                                                                     acaTTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATtaata                             >  1:1563576/1‑100 (MQ=255)
                                                                      caTTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATtaat                              >  1:1068428/1‑98 (MQ=255)
                                                                          aTCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCt                        <  2:1147966/100‑1 (MQ=255)
                                                                          aTCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCt                        <  2:1459353/100‑1 (MQ=255)
                                                                          aTCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCt                        >  1:2445232/1‑100 (MQ=255)
                                                                           tCGACTGAACGCCGGATATGACAAATCCGGCGCTTTGAACATACTACATAATCCCACCTTATTACTCATACCCTTCTATTGCTCTGGATTAATAATTCt                        <  1:2064472/99‑1 (MQ=255)
                                                                             ttcaaatcGCCGGATTTGTCATATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTaa                     >  1:1274162/8‑100 (MQ=37)
                                                                             gACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATtaataagatcgga                     >  2:582863/1‑93 (MQ=255)
                                                                              aCTGAACGCCGGATATGACAAATCCGGCGATTTGAGCATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAAc                    <  1:109281/100‑1 (MQ=255)
                                                                               cTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAAcc                   <  2:1390109/100‑1 (MQ=255)
                                                                                 gAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATAGCCTTATATTGATATGGCTTAATATTTCTTAACCCa                 >  1:2151422/1‑100 (MQ=255)
                                                                                 gAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCa                 >  1:2432254/1‑100 (MQ=255)
                                                                                 gAACGCCGGATATGACAAATCCGGCGATGTGAACATACAACATAATTCCACTTTATTACTCATACCCTTCTATAGCGATGGATTAATAATTCTTAAcaca                 >  1:1936160/1‑100 (MQ=255)
                                                                                 aatcGCCGGATTTGTCATATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCa                 >  1:1851047/4‑100 (MQ=38)
                                                                                  atcGCCGGATTTGTCATATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATACTTCTTAACCCaa                >  1:472460/3‑100 (MQ=255)
                                                                                   tcGCCGGATTTGTCATATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCaaa               >  2:414998/2‑100 (MQ=255)
                                                                                    cGCCGGATTTGTCATATCCGGCGATTTGAACATACAACATAATGCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCaaaa              >  1:400101/1‑100 (MQ=255)
                                                                                    cGCCGGATTTGTCATATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCaaaa              >  2:460739/1‑100 (MQ=255)
                                                                                    cGCCGGATTTGTCATATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCaaaa              >  1:34191/1‑100 (MQ=255)
                                                                                     gCCGGATTTGTCATATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAAt             >  1:939767/1‑100 (MQ=255)
                                                                                     gCCGGATTTGTCATATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAAt             >  1:954254/1‑100 (MQ=255)
                                                                                     gCCGGATTTGTCATATCCGGCGATTTGAACATACAACATAATCCCAACTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAAt             >  1:1438346/1‑100 (MQ=255)
                                                                                     gCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAAt             >  2:1711627/1‑100 (MQ=255)
                                                                                         gATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATggct         >  2:849198/1‑98 (MQ=255)
                                                                                             tGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGAc     >  2:882281/1‑100 (MQ=255)
                                                                                              gACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTa        >  1:2023675/1‑96 (MQ=255)
                                                                                              gACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACt    >  2:1963865/1‑100 (MQ=255)
                                                                                              gACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACt    >  2:1589962/1‑100 (MQ=255)
                                                                                              gACAAATCCGGCGATTTGAACATACAACATAATCCCACCATATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACt    <  1:1539732/100‑1 (MQ=255)
                                                                                              gACAAATCAGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACt    >  1:2445986/1‑100 (MQ=255)
                                                                                                cAAATCCGGCGATTTGAACATACATCATAATCCCACCGTATTACTCATACGCTTCTATTGATATGGATTCATGATTCTTAACCCAAAATGGGCAGAcgc   >  1:1376030/1‑97 (MQ=255)
                                                                                                cAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTcc  >  2:1345306/1‑100 (MQ=255)
                                                                                                  |                                                                                                 
CCACCAGCGGTAACGTCTCTGCTCCCGCGCAGTCTAGCCAGGATGGCGCGCCTGCCGAACCGCAGTAACACATTATCGACTGAACGCCGGATATGACAAATCCGGCGATTTGAACATACAACATAATCCCACCTTATTACTCATACCCTTCTATTGATATGGATTAATAATTCTTAACCCAAAATGGGTAGACTCC  >  NC_000913/1657803‑1657998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: