| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,681,424 | T→C | 19.3% | I150T (ATT→ACT) | folM → | dihydromonapterin reductase, NADPH‑dependent; dihydrofolate reductase isozyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,681,424 | 0 | T | C | 19.3% | 134.0 / 28.2 | 68 | I150T (ATT→ACT) | folM | dihydromonapterin reductase, NADPH‑dependent; dihydrofolate reductase isozyme |
| Reads supporting (aligned to +/- strand): ref base T (32/22); new base C (7/6); total (39/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
ACCATGCGCTGGAAAGATTACTGCGTGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCT > NC_000913/1681325‑1681523 | aCCATGCGCTGGAAAGATTACTGCGTGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACatat < 2:2256704/100‑1 (MQ=255) cATGCGCTGGAAAGATTACTGCGTGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTg > 1:2465932/1‑100 (MQ=255) tGCGCTTGAAAGATTACTGCGTGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCg < 1:668489/100‑1 (MQ=255) tGCGCTGGAAAGATTACTGCGTGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCg > 1:108359/1‑100 (MQ=255) tGCGCTGGAAAGATTACTGCGTGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATACTGCg < 1:1911243/100‑1 (MQ=255) gcTGGAAAGATTACTGCGTGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTAt > 2:2270565/1‑100 (MQ=255) gcTGGAAAGATTACTGCGTGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTAt > 2:1957730/1‑100 (MQ=255) gcTGGAAAGATTACTGCGTGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTAt < 2:1550519/100‑1 (MQ=255) gatGGAAAGATTAATGCGTGGGCACGGACACGCCGCCAGCGAGATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTAt > 2:1753271/3‑100 (MQ=255) tGGAAAGATTACTGCGTGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATACTGCGTAtgc > 1:2082500/1‑100 (MQ=255) aaaGATTACTGCGGGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTAtgctgc > 2:1760215/1‑100 (MQ=255) aaaGATTACTGAGTGGGCACGGTCACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATACTGCGTAtgct < 1:1420827/98‑1 (MQ=255) aaGATTACTGCGTGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTgca < 1:1147926/100‑1 (MQ=255) aaGATTACTGCGTGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTgca < 1:225545/100‑1 (MQ=255) gATTACTGCGTGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTgcaag > 2:1881887/1‑100 (MQ=255) gATTACTGCGTGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTgcaag < 1:2402611/100‑1 (MQ=255) aTTACTGCGTGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTgcaagc < 2:2547673/100‑1 (MQ=255) cTGCGTGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAg > 1:1393188/1‑100 (MQ=255) cTGCGTGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAg > 1:1440609/1‑100 (MQ=255) cTGCGTGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATACTGCGTATGCTGCAAGCAAAg > 1:2128299/1‑100 (MQ=255) cGTGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCgg < 1:944672/100‑1 (MQ=255) gTGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGc < 2:1909608/100‑1 (MQ=255) tGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCa > 2:1515700/1‑100 (MQ=255) tGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCa > 1:2540204/1‑100 (MQ=255) gggCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGCGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCa > 1:2028480/1‑99 (MQ=255) ggCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGGGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACt > 2:642216/1‑100 (MQ=255) gCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTg > 1:831650/1‑100 (MQ=255) aCGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATACTGCGTATGCTGCAAGCAAAGCGGCACTGGa > 1:1793562/1‑100 (MQ=255) acacGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGataat > 1:2585711/1‑100 (MQ=255) acacGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGataat < 2:1018211/100‑1 (MQ=255) cacGCCGCCATCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGataata < 1:1694697/100‑1 (MQ=255) cacGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGataata < 2:59448/100‑1 (MQ=255) acGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATACTGCGTATGCTGCAAGCAAAGCGGCACTGGATAatat < 1:1961930/100‑1 (MQ=255) cgccgcCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATg > 1:406873/1‑100 (MQ=255) cgccgcCAGCGATATCATGCACTTTACCGAATATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAatat < 2:2152376/99‑1 (MQ=255) ccgccAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGAc < 2:267265/100‑1 (MQ=255) cgccAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGAcc < 2:2270622/100‑1 (MQ=255) cgccAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGAAGCGACAAACATATTGCGTATGCTGCAAGCAACGCGGCACTGGATAATATGAc > 1:1338074/1‑99 (MQ=255) atCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATACTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTg < 1:839508/100‑1 (MQ=255) tCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGc > 1:1422418/1‑100 (MQ=255) cATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGcc > 2:921060/1‑100 (MQ=255) cATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGcc > 2:2275870/1‑100 (MQ=255) cATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGcc < 1:848815/100‑1 (MQ=255) tCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATACTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGc > 2:1296732/1‑100 (MQ=255) cACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCCCTGGATAATATGACCCGCTCGTTTGCCCGCa > 2:480908/1‑100 (MQ=255) ttACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCt > 2:1979377/1‑100 (MQ=255) ccGATTATGTGGTGGAGCGCGGTAGCGTCAAACATACTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGc < 1:1785715/100‑1 (MQ=255) acGATTATCCGGTGGCCCCCGGTATCGACAAACATATTGAGAATGCCGCAAGCAAAGCGGGACTGGATAATATGACCCGCTCGTTTGCCCGCAAGATg > 1:390877/2‑98 (MQ=255) cGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCa > 2:1327396/1‑100 (MQ=255) gATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCAc > 2:1221079/1‑100 (MQ=255) aTTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCAcc < 1:2306641/100‑1 (MQ=255) ttccgatctggAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCAc < 2:506888/90‑1 (MQ=255) tgtgGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGaa > 1:146022/1‑100 (MQ=255) tggtggAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGaagt > 1:1473205/1‑100 (MQ=255) tggAGCGCGGTAGCGACAAACATAGTGCGTATGCTGCAAGCAAAGCGGCACTGGATAAGATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGaagtgaa < 1:2009030/100‑1 (MQ=255) tggAGCGCGGTAGCGACAAACATACTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGaagtgaa > 2:1134217/1‑100 (MQ=255) ggAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGaagtgaaa > 1:1114900/1‑100 (MQ=255) gAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGaagtgaaag < 2:2207493/100‑1 (MQ=255) gAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGaagtgaaag > 1:2138084/1‑100 (MQ=255) cgGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAAt > 2:1599101/1‑100 (MQ=255) cgGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAAt > 2:238610/1‑100 (MQ=255) cgGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAAt < 2:1037559/100‑1 (MQ=255) gCGACAAACATACTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTAt > 2:2106111/1‑100 (MQ=255) gCGACAAACATACTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTAt < 1:774353/100‑1 (MQ=255) gACAAACATACTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTg > 2:531395/1‑100 (MQ=255) aCAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCAGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTgc > 1:19335/1‑100 (MQ=255) aaaCATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTgcgc < 1:901883/100‑1 (MQ=255) aaaCATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTgcgc < 2:495276/100‑1 (MQ=255) aaCATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGcc < 1:1361579/100‑1 (MQ=255) atTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGc < 2:1128895/100‑1 (MQ=255) ttGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCt < 2:1017843/100‑1 (MQ=255) | ACCATGCGCTGGAAAGATTACTGCGTGGGCACGGACACGCCGCCAGCGATATCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGATAATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCT > NC_000913/1681325‑1681523 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |