| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,685,197 | T→C | 16.3% | K464K (AAA→AAG) | fumC ← | fumarate hydratase (fumarase C),aerobic Class II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,685,197 | 0 | T | C | 16.3% | 173.8 / 25.6 | 79 | K464K (AAA→AAG) | fumC | fumarate hydratase (fumarase C),aerobic Class II |
| Reads supporting (aligned to +/- strand): ref base T (33/33); new base C (7/6); total (40/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
ATGCCGACAATGTGCAGCACCGTTATAAACCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAGGGCCGCAGCTTTTAAGGTCAGCCCT > NC_000913/1685100‑1685296 | aTGCCGACAATGTGCAGCACCGTTATAAACCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCttt < 2:856034/100‑1 (MQ=255) gCCGACAATGTGCAGCACCGTTATAAACCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCa < 2:828147/100‑1 (MQ=255) ccGACAATGTGCAGCACCGTTATAAACCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCAt > 2:1286016/1‑100 (MQ=255) ccGACAATGTGCAGCACCGTTATAAACCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCAt < 2:242886/100‑1 (MQ=255) ccGACAATGTGCAGCACCGTTATAAACCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCAt < 1:1286010/100‑1 (MQ=255) gACAATGTGCAGCACCGTTATAAACCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATAc < 2:586309/100‑1 (MQ=255) aCAATGTGCAGCACCGTTATAAACCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACt > 1:537688/1‑100 (MQ=255) aaTGTGCAGCACCGTTATAAACCGCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGc < 1:1631569/100‑1 (MQ=255) aTGTGCAGCACCGTTATAAACGTCAGGCGCAGCCGCTTCGCTTAATCATTCCACGGCTGCAACTGTATGTTGCCGATTAACGCCCGGCTTTCATACTGcc > 1:816787/1‑100 (MQ=255) aTGTGCAGCACCGTTATAAACCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCCTTCATACTGcc < 1:218218/100‑1 (MQ=255) tgtgCAGCACCGTTATAAACCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCg < 2:368521/100‑1 (MQ=255) gtgCAGCACCGTTATAAACCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGa < 2:39500/100‑1 (MQ=255) tgCAGCACCGTTATAAACCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGAc > 2:671145/1‑100 (MQ=255) tgCAGCACCGTTATAAACCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGAc > 1:443024/1‑100 (MQ=255) agcaCCGTTATAAACCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCAt > 2:509574/1‑100 (MQ=255) agcaCCGTTATAAACCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCAt > 1:246636/1‑100 (MQ=255) aCCGTTATAAACCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTg > 2:2383259/1‑100 (MQ=255) ccGTTATAAACCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCCTTCATACTGCCGACCATCTGt > 2:1071215/1‑100 (MQ=255) tataAACCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTg < 1:733002/100‑1 (MQ=255) tataAACCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTg > 2:1204976/1‑100 (MQ=255) ataAACCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTgg < 1:2288897/100‑1 (MQ=255) ataAACCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTgg > 2:1781498/1‑100 (MQ=255) taAACCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTgg > 1:1920226/1‑99 (MQ=255) taAACCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGc < 2:2211772/100‑1 (MQ=255) taAACCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGc < 1:633219/100‑1 (MQ=255) aaCCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCg < 1:1715336/100‑1 (MQ=255) aCCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGt > 1:1258852/1‑100 (MQ=255) aCCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGt < 1:1755879/100‑1 (MQ=255) tCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTAcc < 2:1912574/100‑1 (MQ=255) cAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGt < 2:1778941/100‑1 (MQ=255) gCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTc > 1:648712/1‑99 (MQ=255) tCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCCTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCg < 2:1909165/100‑1 (MQ=255) ttGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCtt < 1:2567305/100‑1 (MQ=255) tGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCCAACTCGGCTTc > 1:722574/1‑100 (MQ=255) gttCATTCCACGGCTGCACCTGTATTTTGCATATTAACGCCCGGCTTTCATACTTCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCgg < 1:2193182/93‑1 (MQ=255) gATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTc < 1:558107/99‑1 (MQ=255) gATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCCTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCg > 2:1335639/1‑100 (MQ=255) aTCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGGCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGc < 1:1405537/100‑1 (MQ=255) aTCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGc > 2:274571/1‑100 (MQ=255) aTCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCCTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGc < 1:1856263/100‑1 (MQ=255) tCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCt > 2:451368/1‑100 (MQ=255) tCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCt > 1:845402/1‑100 (MQ=255) tCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCCTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCt < 1:1161369/100‑1 (MQ=255) cATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCCTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTa > 2:1541685/1‑100 (MQ=255) ttCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAg < 1:2344746/100‑1 (MQ=255) ttCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCCTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAg > 1:519803/1‑100 (MQ=255) ccACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGa < 1:305801/99‑1 (MQ=255) ccACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGAt > 2:1636332/1‑100 (MQ=255) ccACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGAt > 2:988440/1‑100 (MQ=255) cACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATa > 2:1638101/1‑100 (MQ=255) cGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATAcc > 1:1045090/1‑100 (MQ=255) cGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCCTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATAcc < 2:919257/100‑1 (MQ=255) gCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATAcccc > 2:1431106/1‑100 (MQ=255) tGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATAcccc < 1:1571464/98‑1 (MQ=255) cACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAgcg < 1:1517491/100‑1 (MQ=255) tATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAggg < 1:294735/100‑1 (MQ=255) tATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAggg < 1:1675715/100‑1 (MQ=255) gTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAGGGCCg > 1:206581/1‑100 (MQ=255) ttGCAGATTAACGCCCGGCCTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAGGGCCGc < 1:1647488/100‑1 (MQ=255) tGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAGGGCCGCa < 1:2254455/100‑1 (MQ=255) cAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAGGGCCGCAGc > 1:927904/1‑100 (MQ=255) cAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAGGGCCGCAGc > 2:2018882/1‑100 (MQ=255) cAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAGGGCCGCAGc > 2:2501537/1‑100 (MQ=255) aGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAGGGCCGCAGCt < 2:479240/100‑1 (MQ=255) aGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAGGGCCGCAGCt > 1:2139789/1‑100 (MQ=255) gATTAACGCCCGGCCTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAGGGCCGCAGCtt > 2:1245484/1‑100 (MQ=255) aTTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAGGGCCGCAGCttt < 2:537690/100‑1 (MQ=255) aTTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAGGGCCGCAGCttt < 2:782022/100‑1 (MQ=255) ttAACGCCCGGCCTTCGTACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAGGGCCGCAGCtttt > 2:777657/1‑100 (MQ=255) tAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAGGGCCGCAGCTTTTa < 1:2304377/100‑1 (MQ=255) aaCGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAGGGCCGCAGCTTTTaa > 1:2153587/1‑100 (MQ=255) aaCGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAGGGCCGCAGCTTTTaa < 1:2501518/100‑1 (MQ=255) cGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAGGGCCGCAGCTTTTAAgg < 1:154881/100‑1 (MQ=255) cGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAGGGCCGCAGCTTTTAAgg > 2:1027598/1‑100 (MQ=255) cGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAGCGCCGCAGCTTTTAAgg > 1:1511101/1‑100 (MQ=255) gCCCGGCTTTCGTACTGCCGACCTTCTGTTTGTGCTTTTCCCAGCTTTCAAACTCGGCTTCGCTAAGTTACCCCAGCGCCAGGTCTGCATCtggtagtg < 1:1822587/99‑8 (MQ=255) gCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAGGGCCGCAGCTTTTAAGGt > 1:768260/1‑100 (MQ=255) gCCCGGCCTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAGGGCCGCAGCTTTTAAGGt > 1:157698/1‑100 (MQ=255) ggCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAGGGCCGCAGCTTTTAAGGTCAGc > 2:865742/1‑100 (MQ=255) cTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAGGGCCGCAGCTTTTAAGGTCAGccc > 2:278039/1‑100 (MQ=255) tttCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAGGGCCGCAGCTTTTAAGGTCAGCCct < 2:510877/100‑1 (MQ=255) | ATGCCGACAATGTGCAGCACCGTTATAAACCTCAGGCGCAGCCGCTTCGTTTGATCATTCCACGGCTGCACCTGTATGTTGCAGATTAACGCCCGGCTTTCATACTGCCGACCATCTGTTCTGGCCGTACCCAGCTGTCAAACTCGGCTTCGCTAAGATACCCCAGCGCAAGGGCCGCAGCTTTTAAGGTCAGCCCT > NC_000913/1685100‑1685296 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |