Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,721,377 | G→A | 18.2% | G38G (GGG→GGA) | ydhJ → | putative membrane fusion protein (MFP) of YdhJK efflux pump |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,721,377 | 0 | G | A | 18.2% | 166.6 / 29.3 | 77 | G38G (GGG→GGA) | ydhJ | putative membrane fusion protein (MFP) of YdhJK efflux pump |
Reads supporting (aligned to +/- strand): ref base G (29/34); new base A (6/8); total (35/42) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.23e-01 |
ATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCT > NC_000913/1721279‑1721475 | aTTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGa > 1:1070700/1‑100 (MQ=255) tAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGaaa < 2:349829/100‑1 (MQ=255) tAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGaaa < 2:349764/100‑1 (MQ=255) aaGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGaaaa > 1:2068262/1‑100 (MQ=255) tATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATAc < 1:1903333/100‑1 (MQ=255) tATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATAc < 2:2535700/100‑1 (MQ=255) tttcctaaacaaTAATTGTAAGGGGATTTTCGGTTCTTGCCGGATGGTGTTTGTGGAATTATTACATGCGATCTCCGTGGAGACGGGACGGGaaaa < 1:123711/89‑1 (MQ=255) tctcAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCGGGATGGTGGCTGTGGAATTATTACAGGCAATCGTCGTGGTCACGCGACGGGAAAATAcgc > 2:380376/1‑98 (MQ=255) tctcAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACgcgc > 1:2362099/1‑100 (MQ=255) tctcAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCGGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACgcgc < 1:1862416/100‑1 (MQ=255) tcAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAg < 2:874226/100‑1 (MQ=255) caacaaTCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGa < 2:1884125/100‑1 (MQ=255) caaTCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACa > 2:643388/1‑100 (MQ=255) caaTCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACa < 2:2167897/100‑1 (MQ=255) caaTCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACa < 1:766131/100‑1 (MQ=255) caaTCATTGTAACGGTAATTACGGTTCTCGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACa > 1:1353090/1‑100 (MQ=255) aaTCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAg > 2:881333/1‑100 (MQ=255) aTCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAgg < 2:2212911/100‑1 (MQ=255) aTCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAgg < 2:1063732/100‑1 (MQ=255) aTTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGttt > 2:694727/1‑100 (MQ=255) aTTGTAGCGGTAAGTGCGGTTCTTGACGGATGGTGGCTGTGGAACTAGTACATGCAGTCTGCGTGGACACGCGACGGGAAAATACGCTCATAACAGGttt > 2:2583739/1‑100 (MQ=255) ttGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTc < 1:254217/100‑1 (MQ=255) ttGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGAAAAATACGCGCAGAACAGGTTTc < 2:577929/100‑1 (MQ=255) gTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCACAGAACAGGTTTCTa > 2:2549729/1‑100 (MQ=255) gCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCa > 2:1066607/1‑100 (MQ=255) ggTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCAcc > 2:781668/1‑100 (MQ=255) ggTAGTTGCGGTACTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGCCACGCCACGGGGAAATACGCGCAGAACAGGTTTCTATCAcc > 2:1702516/1‑100 (MQ=255) tAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCAcccc < 2:1070704/100‑1 (MQ=255) gtagaggTTTTTGACGGATGGTGGCGGTGGAATTATTACGTGCAAAATCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCcac < 1:839607/95‑1 (MQ=255) gTTGCGGTTCTTGCCGGATGGTGGCTGTGGAAGTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCcac < 1:582848/100‑1 (MQ=255) ttGCGGTTCTTGCCGGATGGTGTCTGTGGAATTATTACACGAAATCTCCGTGGACACGCGACGTGAAAATACGCGCAGAACAGTTTTCTATCAccc < 1:360750/96‑1 (MQ=255) tGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGAAAAATACGCGCAGAACAGGTTTCTATCACCCCACAg > 1:1515166/1‑100 (MQ=255) gCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTGCGCGGACACGGGACGGGAAAATACCCGCAGACTCGGTTTCTATCACCCaaca > 1:2296123/1‑98 (MQ=255) gCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGAAAAATACGCGCAGAACAGGTTTCTATCACCCCACAgg > 2:1481802/1‑100 (MQ=255) ggTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGgtg > 1:2438538/1‑100 (MQ=255) ggTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGAAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGgtg > 2:2404059/1‑100 (MQ=255) ttCTTGCCGGATGGTGGCTGTGAAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTc < 1:1371391/100‑1 (MQ=255) tGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGAAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGa < 2:1307534/100‑1 (MQ=255) tGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGAAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGa < 2:1347110/100‑1 (MQ=255) gCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGAc > 1:815572/1‑100 (MQ=255) gCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGAc > 2:747091/1‑100 (MQ=255) gCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGAc < 1:2457452/100‑1 (MQ=255) gATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTAt < 2:1023057/100‑1 (MQ=255) ggtggCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTAttgt > 1:929493/1‑100 (MQ=255) gtggCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTAttgtt > 1:1374457/1‑100 (MQ=255) ggCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGa > 1:577752/1‑100 (MQ=255) gCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAg < 1:1781903/100‑1 (MQ=255) gCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAg < 1:1091331/100‑1 (MQ=255) gtgGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTg > 1:715818/1‑100 (MQ=255) gtgGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTg > 1:2045782/1‑100 (MQ=255) ggAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGaa < 1:616646/100‑1 (MQ=255) ggAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGaa > 2:724706/1‑100 (MQ=255) gAATTATTACATGCAATCTCCGTGGACACGCGACGGAAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAat < 1:486410/100‑1 (MQ=255) gAATTATTACATGCAATCTCCGTGGACACGCGACGGAAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAat < 1:2458193/100‑1 (MQ=255) attattACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAatat < 2:2180238/100‑1 (MQ=255) ttattaCATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGATGTATTGTTGAGCTGAAtata < 2:1329864/100‑1 (MQ=255) attaCATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATaaa < 2:243765/100‑1 (MQ=255) ttaCATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCACACAGGTGTCGGGCCGTATTGTTGAGCTGAATATaaaa > 2:1509172/1‑100 (MQ=255) taCATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAg < 1:2457400/100‑1 (MQ=255) aCATGCAATCTCCGTGGACACGCGACGGAAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGa > 2:2282541/1‑100 (MQ=255) tGCAATCTCCGTGGACACGCGACGGGAAAATACGCACAGAACAGGTTTCTATCACCCCAAAGGTGTCGGGACGTATTGGTGAGCTGCATATTAAAGAc > 1:999795/1‑98 (MQ=255) gCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAAc < 2:1564347/100‑1 (MQ=255) gCAATCTCCGTGGACACGCGACGGAAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAAc > 2:1518149/1‑100 (MQ=255) cAATCTCCGTGGACACGCGACGGAAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAAcc < 2:1708247/100‑1 (MQ=255) tcCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTg < 2:493080/100‑1 (MQ=255) cacGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAAcgc < 2:2139461/100‑1 (MQ=255) cacGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAAcgc > 1:1491584/1‑100 (MQ=255) cgcgACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGttgtctttt < 2:695379/100‑8 (MQ=255) cgcgACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCgg > 2:2436747/1‑100 (MQ=255) cgcgACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGCACATATTGTTGAGCAGAATATAAACAACAACCAGCAGGTTACCGCgg > 1:1931839/1‑100 (MQ=255) cgcgACGGGAAAATACGCGCAGAACAGGATTCTGTCACCCCACAGGTGTCGGGACGTATTGTTGCGCTGAATATAAAAGACACCCAGCTGGTTAAc > 1:1696148/1‑96 (MQ=255) gcgACGGAAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCggg < 1:2404044/100‑1 (MQ=255) cgACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCgggg < 1:2566082/100‑1 (MQ=255) cgACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCgggg < 1:718517/100‑1 (MQ=255) cgACGGAAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCgggg > 2:1928875/1‑100 (MQ=255) cgACGGAAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCgggg < 2:1445042/100‑1 (MQ=255) aCGGGAAAATACGCGCCGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGa < 1:1514570/100‑1 (MQ=255) ggAAAATAGGCGCAGATCAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGAtc < 1:1562351/99‑1 (MQ=255) ggAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGAtct < 1:2486834/100‑1 (MQ=255) ggAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGAtct < 2:2062528/100‑1 (MQ=255) | ATTAAGTATTTCTCAACAATCATTGTAGCGGTAGTTGCGGTTCTTGCCGGATGGTGGCTGTGGAATTATTACATGCAATCTCCGTGGACACGCGACGGGAAAATACGCGCAGAACAGGTTTCTATCACCCCACAGGTGTCGGGACGTATTGTTGAGCTGAATATAAAAGACAACCAGCTGGTTAACGCGGGGGATCT > NC_000913/1721279‑1721475 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |