Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,744,859 G→T 15.1% intergenic (+29/+12) mdtK → / ← ydhQ multidrug efflux system transporter/autotransporter adhesin‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,744,8590GT15.1% 247.9 / 29.7 91intergenic (+29/+12)mdtK/ydhQmultidrug efflux system transporter/autotransporter adhesin‑related protein
Reads supporting (aligned to +/- strand):  ref base G (43/34);  new base T (5/9);  total (48/43)
Fisher's exact test for biased strand distribution p-value = 2.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01

TGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTT  >  NC_000913/1744760‑1744958
                                                                                                   |                                                                                                   
tgatgatgTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTg                                                                                                     >  2:1143634/1‑100 (MQ=255)
 gatgatgTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTgc                                                                                                    <  2:204966/100‑1 (MQ=255)
  atgatgTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAACATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTgcg                                                                                                   <  2:503623/100‑1 (MQ=255)
    gatgTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGcc                                                                                                 >  1:343262/1‑100 (MQ=255)
    gatgTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGcc                                                                                                 <  2:1155328/100‑1 (MQ=255)
    gatgTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGcc                                                                                                 <  2:1416637/100‑1 (MQ=255)
      tgTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTg                                                                                               <  1:2313310/100‑1 (MQ=255)
       gTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGt                                                                                              >  2:1246264/1‑100 (MQ=255)
        ttGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTTCGCCTGTa                                                                                             <  1:2104459/100‑1 (MQ=255)
            gTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTAtttt                                                                                         >  1:503763/1‑100 (MQ=255)
             tATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTAttttt                                                                                        >  1:1963121/1‑100 (MQ=255)
              aTGCGGTTCCTGCAACTTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTAttttt                                                                                        <  1:1511466/99‑1 (MQ=255)
              aTGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTa                                                                                       <  2:872409/100‑1 (MQ=255)
              aTGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTa                                                                                       <  2:1175586/100‑1 (MQ=255)
              aTGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTa                                                                                       <  1:968321/100‑1 (MQ=255)
                gCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACg                                                                                     <  1:2044708/100‑1 (MQ=255)
                 cGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTTCGCCTGTATTTTTACGc                                                                                    <  2:1612191/100‑1 (MQ=255)
                 cGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGc                                                                                    >  2:835874/1‑100 (MQ=255)
                       cTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTg                                                                              >  2:1105230/1‑100 (MQ=255)
                       cTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTg                                                                              <  1:2233500/100‑1 (MQ=255)
                        tGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTTCGCCTGTATTTTTACGCAGGCTgg                                                                             >  2:2179536/1‑100 (MQ=255)
                          cAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAg                                                                           >  1:1624412/1‑100 (MQ=255)
                           aaCGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTTCGCCTGTATTTTTACGCAGGCTGGAGc                                                                          >  2:2534250/1‑100 (MQ=255)
                           aaCGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGc                                                                          <  2:2501353/100‑1 (MQ=255)
                              gTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGtt                                                                       >  2:2436778/1‑100 (MQ=255)
                               tCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTg                                                                      >  2:2241816/1‑100 (MQ=255)
                                cTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTgc                                                                     <  2:264319/100‑1 (MQ=255)
                                  gCCGTCAGCCATCGTTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTgcgc                                                                   >  1:611923/1‑100 (MQ=255)
                                   ccGTCAGCCATCATTCTGCAACGAGCATCTCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTgcgcn                                                                  <  1:416446/100‑2 (MQ=255)
                                   ccGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTTCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGcc                                                                  <  2:961419/100‑1 (MQ=255)
                                   ccGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTgcgc                                                                   <  2:759850/99‑1 (MQ=255)
                                   ccGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGcc                                                                  <  2:890177/100‑1 (MQ=255)
                                   ccGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGcc                                                                  >  2:2339265/1‑100 (MQ=255)
                                   ccGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGcc                                                                  >  2:766950/1‑100 (MQ=255)
                                   ccGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGcc                                                                  <  2:112531/100‑1 (MQ=255)
                                    cGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCa                                                                 >  1:1614221/1‑100 (MQ=255)
                                        aGCCATCATTCTGCAACGAGCAGCCCGCTAATAAAGACAACGCGCAACCTTCACGGGTTGCGCTTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATc                                                              <  1:304625/99‑1 (MQ=255)
                                          ccATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTTCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCg                                                           <  1:367205/100‑1 (MQ=255)
                                          ccATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCg                                                           >  1:2399606/1‑100 (MQ=255)
                                          ccATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCg                                                           >  2:157938/1‑100 (MQ=255)
                                          ccATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCg                                                           >  1:604326/1‑100 (MQ=255)
                                          ccATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCg                                                           <  2:233032/100‑1 (MQ=255)
                                           catcatTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGt                                                          >  2:157110/1‑100 (MQ=255)
                                             tcatTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCt                                                        >  2:1876926/1‑100 (MQ=255)
                                              catTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCtt                                                       >  2:2374921/1‑100 (MQ=255)
                                               atTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTc                                                      >  1:1939026/1‑100 (MQ=255)
                                                ttCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCg                                                     >  2:1607158/1‑100 (MQ=255)
                                                  cTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTTCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTc                                                   <  2:1917599/100‑1 (MQ=255)
                                                    gCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTg                                                 <  2:624648/100‑1 (MQ=255)
                                                    gCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTg                                                 >  1:90393/1‑100 (MQ=255)
                                                    gCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTg                                                 >  2:1467960/1‑100 (MQ=255)
                                                    gCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTg                                                 >  1:2022058/1‑100 (MQ=255)
                                                     cAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTgg                                                <  2:125878/100‑1 (MQ=255)
                                                      aaCGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGc                                               >  2:2076928/1‑100 (MQ=255)
                                                       aCGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCt                                              <  1:1876916/100‑1 (MQ=255)
                                                        cGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTTCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTg                                             >  1:2003804/1‑100 (MQ=255)
                                                        cGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTTCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTg                                             >  1:1679631/1‑100 (MQ=255)
                                                           tcATCCCGCTAATAACGACAAGGCGGAACCTTCACGGGTGGCGTCTGTAATTTTACGGAGGCTGGAGCGTTGCGCCAATCCCGTTGTCGTATGGCTGTa                                           >  1:1997085/2‑99 (MQ=255)
                                                           gCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTa                                           <  1:1890184/99‑1 (MQ=255)
                                                             aTCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAAtt                                        <  2:219495/100‑1 (MQ=255)
                                                              tCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAAttt                                       >  1:2356455/1‑100 (MQ=255)
                                                                cggCTAATAAAGAGAAGGCGCAACCTTCACGGGTTTCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCa                                     >  2:1758950/3‑100 (MQ=255)
                                                                ccGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCa                                     >  1:2075334/1‑100 (MQ=255)
                                                                     aataaAGACAAGGCGCAACCTTCACGGGTTTCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCg                                <  2:1130932/100‑1 (MQ=255)
                                                                     aataaAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCg                                >  1:815432/1‑100 (MQ=255)
                                                                      ataaAGACAAGGCGCAACCTTCACGGGTTTCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTc                                      <  1:309301/93‑1 (MQ=255)
                                                                      ataaAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGt                               >  1:630061/1‑100 (MQ=255)
                                                                        aaaGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTa                             >  2:1360138/1‑100 (MQ=255)
                                                                         aaGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCTCTTATTTGTCTGGCTGTACTTTCAGCGCGTTa                             >  1:2340428/1‑99 (MQ=255)
                                                                          aGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTaca                           >  1:322563/1‑100 (MQ=255)
                                                                           gACAAGGCGCAACCTTCACGGGTTTCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTacac                          <  2:122148/100‑1 (MQ=255)
                                                                           gACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTacac                          >  2:2076226/1‑100 (MQ=255)
                                                                               aGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACagag                      >  2:2489315/1‑100 (MQ=255)
                                                                                ggCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGt                     >  2:1213671/1‑100 (MQ=255)
                                                                                ggCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGt                     >  1:1378211/1‑100 (MQ=255)
                                                                                ggCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGt                     >  2:874442/1‑100 (MQ=255)
                                                                                ggCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGt                     >  2:1920121/1‑100 (MQ=255)
                                                                                ggCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGt                     <  2:2037162/100‑1 (MQ=255)
                                                                                ggCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGt                     <  1:1646567/100‑1 (MQ=255)
                                                                                 gcgcAACCTTCACGGGTTGCGCCGGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCCTTACACagagct                    >  1:2259335/1‑98 (MQ=255)
                                                                                   gcAACCTTCACGGGTTTCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTg                  <  1:873656/100‑1 (MQ=255)
                                                                                   gcAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTg                  <  1:410641/100‑1 (MQ=255)
                                                                                     aacccgtgaaggTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGcc                <  1:1861833/90‑1 (MQ=255)
                                                                                     aaCCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGcc                <  2:197326/100‑1 (MQ=255)
                                                                                     aaCCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGcc                >  2:2088374/1‑100 (MQ=255)
                                                                                     aaCCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGcc                <  2:62866/100‑1 (MQ=255)
                                                                                     aaCCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGcc                <  1:1774729/100‑1 (MQ=255)
                                                                                     aaCCTCAACGGGTTGCGCCTGTCTTTTTACGCAGGCTGGCACGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAAAGTTTGcc                <  1:1773585/100‑1 (MQ=255)
                                                                                             cGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACCGAGTTTGCCGACTGGCa        >  2:2425098/1‑100 (MQ=255)
                                                                                             cGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCa        <  2:1130231/100‑1 (MQ=255)
                                                                                             cGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAAAGTTTGCCGACTGGCa        >  1:1786164/1‑100 (MQ=255)
                                                                                               ggTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATc      <  1:1014509/100‑1 (MQ=255)
                                                                                                 ttGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTg    <  2:1794369/100‑1 (MQ=255)
                                                                                                  tGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGt   <  1:2322972/100‑1 (MQ=255)
                                                                                                   gcgcCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGtt  <  1:1167845/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
TGATGATGTTGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCTGGCTGTAATTTCAGAGCGTTACACAGAGTTTGCCGACTGGCATCTGTT  >  NC_000913/1744760‑1744958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: