| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,755,639 | G→A | 100% | intergenic (‑498/‑59) | ydhZ ← / → pykF | uncharacterized protein/pyruvate kinase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,755,639 | 0 | G | A | 98.3% | 247.3 / ‑4.2 | 80 | intergenic (‑498/‑59) | ydhZ/pykF | uncharacterized protein/pyruvate kinase I |
| Reads supporting (aligned to +/- strand): ref base G (2/0); new base A (37/40); total (40/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGA > NC_000913/1755546‑1755734 | gaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCCGATTCGCTTTCCGACAGTgc > 1:1540083/1‑100 (MQ=255) cacaAGCACACATTCCTCTGCACGCTTTTTTTTTTTTTTTTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCAGACCGTgcgc > 1:2581049/1‑100 (MQ=255) cacaAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgcgc < 2:929663/100‑1 (MQ=255) cacaAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgcgc < 1:2048206/100‑1 (MQ=255) cacaAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgcgc < 2:337837/100‑1 (MQ=255) cacaAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgcg > 1:1206383/1‑99 (MQ=255) caAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGccc > 2:1207499/1‑100 (MQ=255) aaGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGTGCCCa < 2:187642/100‑1 (MQ=255) aaGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCa < 2:187602/100‑1 (MQ=255) aaGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCa > 1:2586556/1‑100 (MQ=255) aGCACACATTCCTCTGCACGCTTTTTTGTTGTCTCCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTGGCTTTCCGCCAGTGCGCCCAg > 1:263125/1‑100 (MQ=255) nacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTCCGCCCAGaaa > 1:2578943/2‑99 (MQ=255) cacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAg < 1:1675877/100‑1 (MQ=255) cacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAg < 2:1281344/100‑1 (MQ=255) acaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGc < 2:831709/100‑1 (MQ=255) aTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCaa < 2:2088927/100‑1 (MQ=255) aTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCaa < 2:1338991/100‑1 (MQ=255) tctGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTtc > 2:929714/1‑100 (MQ=255) cACTCTTTTTCGATGTCACCTAGCCTTAGAGCGAGGCACCGCCACTTTCGTAATACCGGATTCGCGTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTccc < 1:531584/100‑1 (MQ=255) cACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTccc > 1:1896297/1‑100 (MQ=255) cGCTATTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTcc < 1:2387208/97‑1 (MQ=255) ttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTtct > 2:2574455/1‑100 (MQ=255) cGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCa < 2:420308/100‑1 (MQ=255) cGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCa > 1:1235722/1‑100 (MQ=255) gATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCaa > 1:2460183/1‑100 (MQ=255) aTGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAAc > 2:1102493/1‑100 (MQ=255) tCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACt > 1:1758535/1‑98 (MQ=255) tCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTa > 1:844315/1‑100 (MQ=255) tCACCTAGCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTa > 1:1887624/1‑100 (MQ=255) cACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTaa > 1:475052/1‑100 (MQ=255) cACCTATACTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGTGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTaa > 2:1356251/1‑100 (MQ=255) ccTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaag > 1:271292/1‑100 (MQ=255) ccTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaag > 1:609979/1‑100 (MQ=255) ccTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaag < 2:345318/100‑1 (MQ=255) ccTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCCTTCCGACAATGCGCCCAGAAAGCAAGCGTCTCCCATCCTTCTCAACTGaa > 1:1134884/1‑98 (MQ=255) tATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagac > 1:2339295/1‑100 (MQ=255) tATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagac < 2:1552864/100‑1 (MQ=255) aTCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagact > 2:1649127/1‑100 (MQ=255) ccTTAGAGCGAGGCACCACCACTTTCGTAATACCTGATTCGCTTTCCGAAACTCCGCACAGAAAGAAAGTTTCTCACATCCTTCTCAACTTAaagactaa > 1:635718/1‑100 (MQ=255) cTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTCCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagacta > 1:2503600/1‑98 (MQ=255) cTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagcctaag > 1:1185308/1‑100 (MQ=255) gagCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTg > 1:471313/1‑100 (MQ=255) gagCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTg < 2:2303152/100‑1 (MQ=255) gagCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTg < 2:1101038/100‑1 (MQ=255) gagCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTg > 2:264751/1‑100 (MQ=255) aGGCGCCACCACATTCGTAATACCTGATTCGCTTTCCGACAGTGCGCCCAGAAATCAAGTTTCTCCCATCCTTCTCATCTTAAAGACTAAGACTGtaa < 1:1987968/98‑3 (MQ=255) aGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATg < 2:614904/100‑1 (MQ=255) caccaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaa < 2:2327084/100‑1 (MQ=255) caccaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaa > 1:2431610/1‑100 (MQ=255) caccaccacTTTCGTAATACCGCATTCGCTTTCCGACAGTGCGCCCAGAAATCACGTTCCTCCCATCCTTCTCAACTTAACGACTAAGACTCTCacca > 1:1992686/1‑95 (MQ=255) ccaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAACGACTAAGACTGTCATGaaaaa < 1:415831/100‑1 (MQ=255) ccaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaaaa < 1:313218/100‑1 (MQ=255) ccaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaaaa > 2:2260819/1‑100 (MQ=255) gatctcTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAg < 2:1086329/95‑1 (MQ=255) caccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAg < 2:1075778/100‑1 (MQ=255) ccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCATAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGAc < 1:1816536/100‑1 (MQ=255) cacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGAcc < 1:1136177/100‑1 (MQ=255) cTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAATCAAGTTTCTCCCATCCTTCTAAAATTCAAGGCTAAGACTCTCATGAAAAAGa > 1:1685309/1‑96 (MQ=255) cTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGagatc > 1:1086325/1‑96 (MQ=255) tttCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCaaa < 1:2122994/100‑1 (MQ=255) cGTAATACCGGATTCGCTTTCCGACAGTGCGCACAGAGAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAAtt < 1:1645802/100‑1 (MQ=255) tAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGt > 2:1709407/1‑100 (MQ=255) aaTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGtt < 1:1419986/100‑1 (MQ=255) aTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGttt < 2:1872122/100‑1 (MQ=255) aCCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTTAGACTGTCATGAAAAAGACCAAAATTGTTTGc < 2:2462928/100‑1 (MQ=255) aCCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGc < 2:1984573/100‑1 (MQ=255) aCCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGc > 1:2396099/1‑100 (MQ=255) aCCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGc < 2:1567426/100‑1 (MQ=255) cgcaTTTGCGTTCCGACAGTGCGCCCAGAAACCAAGTTTCTCCCATCCTTCTCAACTTAAAGACGAAGACTTTCATGACAAAGACCAAAATTGttt < 2:1925168/93‑1 (MQ=255) cGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCAc < 2:1896307/100‑1 (MQ=255) ggATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCAcc < 2:2200729/100‑1 (MQ=255) ggATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCAcc > 2:831843/1‑100 (MQ=255) gATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCa > 2:820670/1‑100 (MQ=255) tCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCg < 2:8454/100‑1 (MQ=255) cGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCgg > 2:213278/1‑100 (MQ=255) cGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCgg > 1:250897/1‑100 (MQ=255) tttCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAcc < 2:658815/100‑1 (MQ=255) tttCCGACAGTGCGCACAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAcc < 1:1506661/100‑1 (MQ=255) ttCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCg < 2:1294182/100‑1 (MQ=255) tCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGa > 2:1136638/1‑100 (MQ=255) | GACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGA > NC_000913/1755546‑1755734 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |