Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,797,391	A→C	16.9%	L185R (CTG→CGG)	pheT ←	phenylalanine tRNA synthetase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,797,391	0	A	C	16.9%	213.4 / 39.0	95	L185R (CTG→CGG)	pheT	phenylalanine tRNA synthetase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (42/36);  new base C (3/13);  total (45/50)
Fisher's exact test for biased strand distribution p-value = 1.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTT  >  NC_000913/1797292‑1797490                                                                                                    |                                                                                                    ccAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCa                                                                                                     <  1:1245723/100‑1 (MQ=255)   aaGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGc                                                                                                   <  2:771272/100‑1 (MQ=255)   aaGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGc                                                                                                   >  1:2495911/1‑100 (MQ=255)    aGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCt                                                                                                  >  2:1496219/1‑100 (MQ=255)    aGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCt                                                                                                  >  2:1081348/1‑100 (MQ=255)       tAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGt                                                                                               >  2:798829/1‑100 (MQ=255)       tAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGt                                                                                               <  2:1576336/100‑1 (MQ=255)       tAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGt                                                                                               <  2:1300664/100‑1 (MQ=255)        aaCGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGt                                                                                               >  1:1625632/1‑99 (MQ=255)        aaCGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGCTGAACCAGCTGCCGCTGGtt                                                                                              <  2:54046/100‑1 (MQ=255)         aCGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTc                                                                                             >  1:1348268/1‑100 (MQ=255)         aCGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTc                                                                                             >  2:1881802/1‑100 (MQ=255)          cgcgGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGCACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCa                                                                                            >  1:2498037/1‑100 (MQ=255)          cgcgGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCa                                                                                            >  1:1213235/1‑100 (MQ=255)          cgcgGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCa                                                                                            >  1:2115422/1‑100 (MQ=255)          cgcgGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCa                                                                                            >  1:1194137/1‑100 (MQ=255)            cgGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGATCGATTTCCGGTTGAACCAGCTGCCGCTGGtt                                                                                              <  1:2577870/96‑1 (MQ=255)            cgGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGTTCaac                                                                                          <  2:1469451/100‑3 (MQ=255)            cgGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGTTCaac                                                                                          <  2:1653609/100‑3 (MQ=255)             gggCCGGCTTCCGGGGCTTCGGCTGTTATCGGCGGCGTGTCGTTGATGGTCGCGCCACCCGGAACGATTTCCGGTTGAACCCGCTGCCGCTGGTTCaac                                                                                          <  1:1055640/99‑3 (MQ=255)             gggCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGACCGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCa                                                                                         <  2:1822836/100‑1 (MQ=255)             gggCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGTTCaacc                                                                                         <  2:314789/100‑4 (MQ=255)             gggCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGTTCaacc                                                                                         <  2:1386981/100‑4 (MQ=255)             gggCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGTTCaacc                                                                                         <  2:2317181/100‑4 (MQ=255)             gggCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGCTGAACCAGCTGCCGCTGGTTCaacc                                                                                         <  2:1235609/100‑4 (MQ=38)             gggCAGGCTTACGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGTTCaacc                                                                                         <  2:84300/100‑4 (MQ=255)             gggCAGGCCTCCGGCGCTGCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGTTCaacc                                                                                         <  1:727812/100‑4 (MQ=255)              ggCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGTTCaacc                                                                                         <  1:722184/99‑4 (MQ=255)                cAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACgg                                                                                      >  2:1002837/1‑100 (MQ=255)                cAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACgg                                                                                      >  2:864421/1‑100 (MQ=255)                   gCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCaa                                                                                   >  1:2008836/1‑100 (MQ=255)                      ttccgcgcTTCGACTGTAGTCTGCAGCGTGTCGTCGATGGTCGCACGAACCGGAACGATGTCCGTTTGAACCAGCTGCCGCTGGTTCaacc                                                                                         <  1:57318/87‑4 (MQ=25)                       ccGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAGCGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACg                                                                                 >  2:520464/1‑98 (MQ=255)                         ggCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCAc                                                                             <  2:1594986/100‑1 (MQ=255)                              ttCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCa                                                                        >  2:1279764/1‑100 (MQ=255)                              ttCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCa                                                                        <  2:2008847/100‑1 (MQ=255)                                  aCTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAacac                                                                    <  2:892570/100‑1 (MQ=255)                                   cTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACAcc                                                                   >  2:150322/1‑100 (MQ=255)                                    tGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAGCCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCATGGCAACGTCACGCGCAacacn                                                                   <  1:166723/99‑2 (MQ=255)                                     gcaaTCGGCAGCGTGTCGTCGATCGTCGCACCAAGCGGAACGATTGCCGGGTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAactc                                                                    <  1:1037503/95‑3 (MQ=255)                                      tAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAat                                                                >  2:94980/1‑100 (MQ=255)                                      tAATCGGCAGCGTGTCGTCGATGGTCGCACCAACAGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCaa                                                                 >  1:909327/1‑99 (MQ=255)                                         tCGGCAGCGTGTCGTCGATGGGCGCACCAAGCGGAAGGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAatga                                                              <  1:1749842/99‑1 (MQ=255)                                          cGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAatgatg                                                            <  1:1071967/100‑1 (MQ=255)                                          cGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAatgatg                                                            >  2:942428/1‑100 (MQ=255)                                           ggCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGc                                                           >  1:868782/1‑100 (MQ=255)                                               gCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTa                                                        <  1:337584/99‑1 (MQ=255)                                                 gtgtCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGc                                                     >  1:1066716/1‑100 (MQ=255)                                                 gtgtCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGc                                                     <  2:1569512/100‑1 (MQ=255)                                                 gtgtCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGc                                                     <  2:1499107/100‑1 (MQ=255)                                                   gtcgtcGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAg                                                   <  2:650443/100‑1 (MQ=255)                                                       tcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCgg                                               <  1:673390/100‑1 (MQ=255)                                                        cgATGGTCGCAGCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGc                                              >  1:1938479/1‑100 (MQ=255)                                                        cgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGc                                              >  2:760466/1‑100 (MQ=255)                                                        cgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGc                                              <  1:1002570/100‑1 (MQ=255)                                                                  aCCAACCGGAACGATTTCCGGTGGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTGACGCGCAACACGAATGATGCCTAAGCAGTCCGCACGGttt                                       >  1:637124/1‑97 (MQ=255)                                                                   ccAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCg                                   >  1:2332838/1‑100 (MQ=255)                                                                    cAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGt                                  >  1:2174851/1‑100 (MQ=255)                                                                     aaCCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTc                                 <  2:785533/100‑1 (MQ=255)                                                                     aaCCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGGCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCg                                   <  1:850743/98‑1 (MQ=255)                                                                       ccGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCAc                               >  2:312597/1‑100 (MQ=255)                                                                       ccGGAACGATTTCCGGTTGAACCAGCGGCAGCGGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCAc                               <  2:1838323/100‑1 (MQ=255)                                                                         ggaaatcgttCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGc                             >  1:1415915/9‑100 (MQ=255)                                                                         ggAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCCACACCCATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGc                             >  1:2421162/1‑100 (MQ=255)                                                                         ggAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGc                             >  2:2005376/1‑100 (MQ=255)                                                                         ggAACGATTCCCGTTGGAACCGTCGGCAGCTGGTTCAGCACGGCAACGACACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACgg                             <  1:2450239/100‑2 (MQ=255)                                                                         gcaaCGATTTCCGGTTGAACCCGCGGCAGCTGCTTCAGCACGGCAACGTCACGCGCAACCCCAATGATGCCTAAGCAGTCGGCACGGTTTGGc                                    <  1:680642/91‑1 (MQ=255)                                                                           aaCGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACATCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTg                           <  1:1410301/100‑1 (MQ=255)                                                                           aaCGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTg                           <  2:868785/100‑1 (MQ=255)                                                                               aTTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGAttt                       >  2:890147/1‑100 (MQ=255)                                                                                tttCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTc                      >  2:1426653/1‑100 (MQ=255)                                                                                tttCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTc                      <  1:291925/100‑1 (MQ=255)                                                                                tttCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAACCATGCCTAAGCAGTCGGCACGGTTTGGCGCCACGCTGATTTc                      <  1:292950/100‑1 (MQ=255)                                                                                   ccGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGAt                   <  2:1760536/100‑1 (MQ=255)                                                                                    cGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATg                  <  2:2059897/100‑1 (MQ=255)                                                                                     ggTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATgg                 <  2:671130/100‑1 (MQ=255)                                                                                     ggTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATgg                 >  1:667767/1‑100 (MQ=255)                                                                                      gTTGAACCAGCGGCGGCTGGGTCAGCACGACAGCGTCACGCGCAACCCCAAGGATGGCTAAGCAGGCGGCACGGTTTGGCGTCACGCTGATTTAGAt                   <  1:789034/97‑1 (MQ=255)                                                                                       ttGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGgtg               <  2:1086250/100‑1 (MQ=255)                                                                                       ctGAACCAGCTGCCGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGgtg               >  2:1083808/2‑100 (MQ=255)                                                                                       ccgAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGgtg               >  2:1408568/3‑100 (MQ=255)                                                                                        tGAACCAGCTGCCGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGgtgt              >  2:2386618/1‑100 (MQ=255)                                                                                        tGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGgtgt              <  1:696504/100‑1 (MQ=255)                                                                                         gAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGtgtt             <  2:2155124/100‑1 (MQ=255)                                                                                          aaCCAGCTGCCGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGtgttg            >  2:984169/1‑100 (MQ=255)                                                                                          aaCCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGtgttg            >  2:658823/1‑100 (MQ=255)                                                                                           aCCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGtgttgt           >  1:77119/1‑100 (MQ=255)                                                                                           aCCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGtgttgt           >  2:2282757/1‑100 (MQ=255)                                                                                           aCCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGtgttgt           >  1:1190167/1‑100 (MQ=255)                                                                                             cAGCGGTAGCTGGTTCAGCACGGCAACGTTACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGtca         <  1:740127/100‑1 (MQ=255)                                                                                             cAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGtca         <  1:1881490/100‑1 (MQ=255)                                                                                              aGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGtcat        >  1:1215577/1‑100 (MQ=255)                                                                                              aGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGtcat        >  2:542851/1‑100 (MQ=255)                                                                                                 ggCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCaa     >  1:221327/1‑100 (MQ=255)                                                                                                  gCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAg    <  2:1190171/100‑1 (MQ=255)                                                                                                   cAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGt   <  2:69350/100‑1 (MQ=255)                                                                                                    aGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGtt  <  2:1774242/100‑1 (MQ=255)                                                                                                    |                                                                                                    CCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTT  >  NC_000913/1797292‑1797490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑