| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,871,271 | A→G | 18.7% | Y296C (TAT→TGT) | yeaI → | putative membrane‑anchored diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,871,271 | 0 | A | G | 18.7% | 152.7 / 26.9 | 74 | Y296C (TAT→TGT) | yeaI | putative membrane‑anchored diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base A (33/27); new base G (7/7); total (40/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAGCTGCAACTCTCCAGCAAACTGGCAG > NC_000913/1871172‑1871369 | ccAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGta < 1:1737981/100‑1 (MQ=255) cAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGtat > 2:1763854/1‑100 (MQ=255) cAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGtat > 1:331772/1‑100 (MQ=255) aGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGtata < 2:2134995/100‑1 (MQ=255) cGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGGATATc < 1:1643073/100‑1 (MQ=255) aaCTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACGCTGGATGAGTATAATTATACTATCTGGTGTATCAg < 1:2441095/100‑1 (MQ=255) tGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTcgcg > 2:2442747/1‑100 (MQ=255) gCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTTTACTGACTCTGGATGAGTCGAATTATACTATCTGGTATATCAGTcgcg > 1:2324105/1‑98 (MQ=255) gCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCggg > 1:470301/1‑100 (MQ=255) aTAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTGTATCAGTCGCGGGATTGAAg > 2:1494326/1‑100 (MQ=255) aTAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAg < 1:1547105/100‑1 (MQ=255) aGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTGTATCAGTCGCGGGATTGAAGtt > 2:860584/1‑100 (MQ=255) gCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGttt > 2:2343262/1‑100 (MQ=255) cATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTc > 2:864110/1‑100 (MQ=255) attattATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTGTATCAGTCGCGGGATTGAAGTTTcc < 1:1612865/100‑1 (MQ=255) attattATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTGTATCAGTCGCGGGATTGAAGTTTcc < 2:1170911/100‑1 (MQ=255) attattATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTcc < 2:1900837/100‑1 (MQ=255) attatGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTGTATCAGTCGCGGGATTGAAGTTTCCAGt < 2:1411762/100‑1 (MQ=255) tatGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTaa > 1:1994099/1‑100 (MQ=255) tGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAAc < 1:493727/100‑1 (MQ=255) tGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAAc < 1:1811458/100‑1 (MQ=255) gTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATTAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACt < 2:994602/100‑1 (MQ=255) ncTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACtg > 2:2567687/2‑100 (MQ=255) cTGGCATCGCTATGCTGCAACTTACTTCTACAGAATCTGGATGAGTATAATTATACTATCTAGTGTATCAGTCGCAGGATTCAAGTTTCCAGTAAACtg < 1:380839/99‑1 (MQ=255) tGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTGTATCAGTCGCGGGATTGAAGTTTCCAGTAAACtgtt > 2:204502/1‑100 (MQ=255) ggCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTGTATCAGTCGCGGGATTGAAGTTTCCAGTAAACtgtt > 1:1257353/1‑99 (MQ=255) cATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCATTCGCTGGATTGAATTTTCCAGTAAAAtgttttt > 1:934133/1‑98 (MQ=255) cATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACtgtttgt > 1:2115650/1‑100 (MQ=255) tCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTttgttg < 2:1814907/100‑1 (MQ=255) cGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTtgttgt < 2:1645987/100‑1 (MQ=255) gCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGAGTGAAGTTTCCAGTAAACTGTTTGTtgtg > 1:2563248/1‑100 (MQ=255) gctgcAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCttt > 1:1007223/1‑100 (MQ=255) ctgcAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCtttt > 2:1764580/1‑100 (MQ=255) ctgcAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCtttt > 2:1157388/1‑100 (MQ=255) tgcAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTc > 1:671689/1‑100 (MQ=255) gcAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCt < 1:629665/100‑1 (MQ=255) tacttCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTta < 1:1323443/100‑1 (MQ=255) tacttCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTta > 2:1549365/1‑100 (MQ=255) acttCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTtat < 2:1358936/100‑1 (MQ=255) cttCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTtata > 1:379569/1‑100 (MQ=255) cttCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTtata > 1:161674/1‑100 (MQ=255) cttCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTtata < 2:156277/100‑1 (MQ=255) cttCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTtata > 2:1227271/1‑100 (MQ=255) ttCTACTGACTCTGGATGAGTATAATTATACTATCTGCTATATCAGTCGCGGGATTCAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATaa > 1:1677014/1‑100 (MQ=255) cTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACa > 1:2546110/1‑100 (MQ=255) tGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACAtttt < 2:850546/100‑1 (MQ=255) tGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACAtttt < 1:1764571/100‑1 (MQ=255) gACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACAttttt > 1:1250671/1‑100 (MQ=255) gACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACAttttt > 2:1056550/1‑100 (MQ=255) ctctGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTc < 1:1476166/99‑1 (MQ=255) tctGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAg < 2:1999923/100‑1 (MQ=255) ctGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAg > 1:1811639/1‑99 (MQ=255) tGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGGTTATTATGACTTTTCTGTTTTATAACATTTTTCAg > 1:192026/1‑98 (MQ=255) ggATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAg > 1:2125575/1‑100 (MQ=255) ggATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTCGTGTCTTTTCTGATTTATAACATTTTTCAGGAg > 1:2245193/1‑100 (MQ=255) aTGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAGCt < 1:1620059/100‑1 (MQ=255) gAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAGCTGc < 1:712408/100‑1 (MQ=255) aGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAGCTGCa < 2:1470366/100‑1 (MQ=255) gTATAATTATACTATCTGGTGTATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAGCTGCaa < 2:2079661/100‑1 (MQ=255) tataATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAGCTGCAAc < 2:291594/100‑1 (MQ=255) tataATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAGCTGCAAc < 2:1633164/100‑1 (MQ=255) tataATTATACTATCTGGTATATCAGTCGCGGAATTGAAGTTTCCAGTAAACTGTTTGTTGTGGCTTTTCTGATTTATAACATTTTTCAGGAGCTGCAAc > 1:428467/1‑100 (MQ=255) aaTTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAGCTGCAActct > 1:56316/1‑100 (MQ=255) aTTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAGCTGCAACtctc > 1:1399560/1‑100 (MQ=255) ttATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAGCTGCAACTCTcc < 2:2546129/100‑1 (MQ=255) tataCTATCTGGTGTATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAGCTGCAACTCTCCa > 1:1533212/1‑100 (MQ=255) tataCTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAGCTGCAACTCTCCa < 2:1091257/100‑1 (MQ=255) taCTATCTGGTGTATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAGCTGCAACTCTCCAGc < 1:1933258/100‑1 (MQ=255) aCTATCTGGTGTATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAGCTGCAACTCTCCAGCa > 2:413004/1‑100 (MQ=255) aCTATCTGGTATATCAGTCGCGGTTTTGAAGTTTCCAGTAAAGTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGAACCTGCAACTCTCCAGCa > 1:935547/1‑100 (MQ=255) cTATCTGGTATATCAGTCGTGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAGCTGCAACTCTCCAGCaa > 1:650256/1‑100 (MQ=255) cTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAGCTGCAACTCTCCAGCaa > 2:899615/1‑100 (MQ=255) cTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAGCTGCAACTCTCCAGCaa > 1:156623/1‑100 (MQ=255) tCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAGCTGCAACTCTCCAGCAAACt < 1:270434/100‑1 (MQ=255) cTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAGCTGCAACTCTCCAGCAAACTg < 2:1915776/100‑1 (MQ=255) tGGTGTATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAGCTGCAACTCTCCAGCAAACTgg > 2:1866337/1‑100 (MQ=255) ggTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAGCTGCAACTCTCCAGCAAACTGGc < 1:1549356/100‑1 (MQ=255) ggTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAGCTGCAACTCTCCAGCAAACTGGc < 1:416551/100‑1 (MQ=255) tataTCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAGCTGCAACTCTCCAGCAAACTGGCAg > 1:1426596/1‑100 (MQ=255) | CCAGCGAACTATGGCCGTTAATAGCATTATTATGTCTGGCATCGCTATGCTGCAACTTACTTCTACTGACTCTGGATGAGTATAATTATACTATCTGGTATATCAGTCGCGGGATTGAAGTTTCCAGTAAACTGTTTGTTGTGTCTTTTCTGATTTATAACATTTTTCAGGAGCTGCAACTCTCCAGCAAACTGGCAG > NC_000913/1871172‑1871369 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |