Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,965,088	C→T	17.2%	V368V (GTG→GTA) ‡	flhB ←	flagellin export apparatus, substrate specificity protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,965,088	0	C	T	17.2%	212.0 / 32.4	94	V368V (GTG→GTA)	flhB	flagellin export apparatus, substrate specificity protein
Reads supporting (aligned to +/- strand):  ref base C (47/30);  new base T (8/8);  total (55/39)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.97e-01

ATCTGCCATTGTGTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTA  >  NC_000913/1964989‑1965186                                                                                                    |                                                                                                   aTCTGCCATTGTGTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGc                                                                                                    <  2:1186310/100‑1 (MQ=255)    tGCCATTGTGTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCAcc                                                                                                 >  2:960608/1‑100 (MQ=255)     gCCATTGTGTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTAGTTAATAAAATCCAGGGCTTCCGGCACCg                                                                                                >  1:592615/1‑100 (MQ=255)      ccATTGTGTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCgg                                                                                               <  2:1489281/100‑1 (MQ=255)       cATCGTGTCGATCTCAGGTTTGCGGGCCGGCGCAGCATCGCGGCCAGAATACTGTAGGGTCGGTGTCGCGCAAATAAAATCCAGGGCTCCCGGCACCga                                                                                               >  1:1288297/1‑98 (MQ=255)        aTTGTGTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGaa                                                                                             <  2:1103688/100‑1 (MQ=255)          tgtgTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGa                                                                                           >  1:1096098/1‑100 (MQ=255)           gtgtCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGAt                                                                                          <  2:864019/100‑1 (MQ=255)             gtCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGTGCCGGAAgccct                                                                                        <  1:165427/100‑5 (MQ=255)             gtCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGa                                                                                        >  2:73244/1‑100 (MQ=255)              tCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGTGCCGGAAgccctg                                                                                       <  2:2529422/100‑6 (MQ=255)              tCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGTGCCGGAAgccctg                                                                                       <  1:1656269/100‑6 (MQ=255)               cGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGTGCCGGAAgccctgg                                                                                      <  1:2361924/100‑7 (MQ=255)               cGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGTGCCGGAAgccctgg                                                                                      <  1:1456516/100‑7 (MQ=255)               cGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGTGCCGGAAgccctgg                                                                                      <  1:1454915/100‑7 (MQ=255)               cGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGt                                                                                      >  2:2146237/1‑100 (MQ=255)               cGATTTCAGGTTTGCGGGCAGGCGCAGCATCACGGCCAGATTACTCCTGGGTGGGTTTCTCGTTAGTAAAATCCAGGGCTGCCGCCACCGCAAGGTg                                                                                         >  1:1730318/1‑97 (MQ=255)               cGATTGCAGGTTGGCGGGCAGGTGCAGCATCGCGGCCAGATTACTCATGGGTCGGCTTCTCGTTAATAAAATCCAGGGCTTCCTGGGCCGGAAgccctgg                                                                                      <  1:2085131/100‑7 (MQ=255)                gATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGTGCCGGAAgccctgga                                                                                     <  1:730290/100‑8 (MQ=255)                gATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGTGCCGGAAgccctgga                                                                                     <  2:407045/100‑8 (MQ=255)                gATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTa                                                                                     >  1:189405/1‑100 (MQ=255)                  tttCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAgg                                                                                   <  2:632032/100‑1 (MQ=255)                    tCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCCCCGGAAGATGAGTAGGt                                                                                  >  1:2120914/1‑99 (MQ=255)                     cAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTg                                                                                >  1:1952668/1‑100 (MQ=255)                     cAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTg                                                                                >  2:606990/1‑100 (MQ=255)                     cAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTg                                                                                <  2:1198417/100‑1 (MQ=255)                       ggTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTa                                                                              <  2:623871/100‑1 (MQ=255)                         tttGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACa                                                                            >  2:704332/1‑100 (MQ=255)                              gggCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGgcg                                                                       >  2:1655220/1‑100 (MQ=255)                               ggCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGgcgc                                                                      >  1:598274/1‑100 (MQ=255)                               ggCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTGCCGGCACCGGAAGATGAGTAGGTTGTACAGGgcgc                                                                      <  1:1167260/100‑1 (MQ=255)                                  aGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGt                                                                   <  2:408575/100‑1 (MQ=255)                                  aGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGt                                                                   <  1:630047/100‑1 (MQ=255)                                  aGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGt                                                                   <  1:2526812/100‑1 (MQ=255)                                     cgcAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTg                                                                    <  2:132194/96‑1 (MQ=255)                                       cagcaTCGCGGCCAGATTACGCATTGGTTGGTTTGTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTccacc                                                              <  1:190797/100‑1 (MQ=255)                                        agcaTCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTccacca                                                             >  2:107573/1‑100 (MQ=255)                                           aTCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCAccagcc                                                          >  2:2111948/1‑100 (MQ=255)                                           aTCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCAccagcc                                                          <  2:678915/100‑1 (MQ=255)                                           aTCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCAccagcc                                                          <  2:643526/100‑1 (MQ=255)                                             cgcgGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCAccagccag                                                        >  2:1104095/1‑100 (MQ=255)                                             cgcgGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGGTGTACCGGGCGCTGTCCAccagccag                                                        >  1:1129424/1‑100 (MQ=255)                                               cgGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGAc                                                      >  2:461547/1‑100 (MQ=255)                                                ggCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACg                                                     >  2:56671/1‑100 (MQ=255)                                                ggCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACg                                                     <  2:1311518/100‑1 (MQ=255)                                                 gCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGc                                                    >  1:572803/1‑100 (MQ=255)                                                          cTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTc                                           >  1:156603/1‑100 (MQ=255)                                                            cATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAg                                         >  1:775126/1‑100 (MQ=255)                                                            cATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAg                                         <  2:192354/100‑1 (MQ=255)                                                            cATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACAAGCCAGACGCCAGCGTTTCAg                                         >  1:1788540/1‑100 (MQ=255)                                                            cATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTCGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAg                                         >  1:494019/1‑100 (MQ=255)                                                            cATGGGTCGGTCTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAAGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAg                                         >  1:1361147/1‑100 (MQ=255)                                                               gggTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTg                                      <  2:754402/100‑1 (MQ=255)                                                                ggTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGc                                     >  1:489047/1‑100 (MQ=255)                                                                 gTCGGTTTCTAGTTAATAAAATCCAGGGCTGCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGcc                                    <  1:2406423/100‑1 (MQ=255)                                                                   cGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAg                                  <  1:177165/100‑1 (MQ=255)                                                                    ggTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGcc                                    >  1:2328626/1‑97 (MQ=255)                                                                      tttCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGAcc                               <  1:73243/100‑1 (MQ=255)                                                                           cGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCATCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGc                          >  1:1358936/1‑100 (MQ=255)                                                                            gTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGcc                         >  2:2294915/1‑100 (MQ=255)                                                                            gTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGcc                         <  2:2018854/100‑1 (MQ=255)                                                                             ttAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCa                        <  1:2146223/100‑1 (MQ=255)                                                                               tataaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGc                      <  2:1239877/99‑1 (MQ=255)                                                                                ataaAATGCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCa                     <  1:1391768/100‑1 (MQ=255)                                                                                ataaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCa                     >  2:437903/1‑100 (MQ=255)                                                                                ataaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCa                     <  1:960605/100‑1 (MQ=255)                                                                                ataaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCa                     >  1:1239873/1‑100 (MQ=255)                                                                                ataaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCa                     >  2:2099165/1‑100 (MQ=255)                                                                                ataaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCa                     >  2:2384405/1‑100 (MQ=255)                                                                                ataaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCa                     >  2:530771/1‑100 (MQ=255)                                                                                 taaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCAc                    <  2:28566/100‑1 (MQ=255)                                                                                 taaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCAc                    >  2:1166076/1‑100 (MQ=255)                                                                                  aaaaTCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACt                   >  2:167027/1‑100 (MQ=255)                                                                                   aaaTCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACtt                  >  1:971834/1‑100 (MQ=255)                                                                                    cctactcatcTTCCGGTGCCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTc                 >  2:1107704/10‑100 (MQ=255)                                                                                     ctactcatcTTCCGGTGCCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCCCCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTcc                >  1:584338/9‑100 (MQ=255)                                                                                     aTCCTGGGCTTCCGGCACAGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCGAGACTCAGGCCAGCACTTcc                >  1:2290311/1‑100 (MQ=255)                                                                                     aTCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTcc                >  1:2248453/1‑100 (MQ=255)                                                                                      tCCATGGCTTCCGGCACCGGAAGATGAGTAGGGTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTcc                <  1:108014/99‑1 (MQ=255)                                                                                      tCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCg               <  2:475867/100‑1 (MQ=255)                                                                                       actcatcTTCCGGTGCCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCCCCAGCCAGACGCTAGCGTGTCAGTCGCCAGACCCTCGCCAGCACTTcc                >  1:962463/7‑98 (MQ=255)                                                                                        cAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAAGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGcc             >  2:2259453/1‑100 (MQ=255)                                                                                          catcTTCCGGTGCCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCAc           >  2:1718855/4‑100 (MQ=255)                                                                                          catcTTCCGGTGCCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTCCCGCCAc           >  2:2293131/4‑100 (MQ=255)                                                                                           ggCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCAcc          >  1:1299380/1‑100 (MQ=255)                                                                                           ggCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCAcc          >  2:1765856/1‑100 (MQ=255)                                                                                           atcTTCCGGTGCCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCAcc          >  1:537220/3‑100 (MQ=255)                                                                                           atcTTCCGGTGCCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCAcc          >  2:1853370/3‑100 (MQ=255)                                                                                             cTTCCGGTGCCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACcgc        >  2:138722/1‑100 (MQ=255)                                                                                             cTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACcgc        >  2:427433/1‑100 (MQ=255)                                                                                             cTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACcgc        <  2:561268/100‑1 (MQ=255)                                                                                             cTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACcgc        >  1:1998877/1‑100 (MQ=255)                                                                                                  ggCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGt   >  1:917074/1‑100 (MQ=255)                                                                                                  ggCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGt   <  2:652519/100‑1 (MQ=255)                                                                                                   gCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTa  >  1:1476987/1‑100 (MQ=255)                                                                                                    |                                                                                                   ATCTGCCATTGTGTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTA  >  NC_000913/1964989‑1965186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑