Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,965,089	A→G	18.1%	V368A (GTG→GCG) ‡	flhB ←	flagellin export apparatus, substrate specificity protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,965,089	0	A	G	18.1%	188.0 / 34.5	94	V368A (GTG→GCG)	flhB	flagellin export apparatus, substrate specificity protein
Reads supporting (aligned to +/- strand):  ref base A (46/30);  new base G (8/9);  total (55/39)
Fisher's exact test for biased strand distribution p-value = 4.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01

TGCCATTGTGTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACA  >  NC_000913/1964992‑1965188                                                                                                  |                                                                                                    tGCCATTGTGTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCAcc                                                                                                   >  2:960608/1‑100 (MQ=255)  gCCATTGTGTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTAGTTAATAAAATCCAGGGCTTCCGGCACCg                                                                                                  >  1:592615/1‑100 (MQ=255)   ccATTGTGTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCgg                                                                                                 <  2:1489281/100‑1 (MQ=255)    cATCGTGTCGATCTCAGGTTTGCGGGCCGGCGCAGCATCGCGGCCAGAATACTGTAGGGTCGGTGTCGCGCAAATAAAATCCAGGGCTCCCGGCACCga                                                                                                 >  1:1288297/1‑98 (MQ=255)     aTTGTGTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGaa                                                                                               <  2:1103688/100‑1 (MQ=255)       tgtgTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGa                                                                                             >  1:1096098/1‑100 (MQ=255)        gtgtCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGAt                                                                                            <  2:864019/100‑1 (MQ=255)          gtCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGTGCCGGAAgccct                                                                                          <  1:165427/100‑5 (MQ=255)          gtCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGa                                                                                          >  2:73244/1‑100 (MQ=255)           tCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGTGCCGGAAgccctg                                                                                         <  1:1656269/100‑6 (MQ=255)           tCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGTGCCGGAAgccctg                                                                                         <  2:2529422/100‑6 (MQ=255)            cGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGTGCCGGAAgccctgg                                                                                        <  1:2361924/100‑7 (MQ=255)            cGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGTGCCGGAAgccctgg                                                                                        <  1:1454915/100‑7 (MQ=255)            cGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGTGCCGGAAgccctgg                                                                                        <  1:1456516/100‑7 (MQ=255)            cGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGt                                                                                        >  2:2146237/1‑100 (MQ=255)            cGATTTCAGGTTTGCGGGCAGGCGCAGCATCACGGCCAGATTACTCCTGGGTGGGTTTCTCGTTAGTAAAATCCAGGGCTGCCGCCACCGCAAGGTg                                                                                           >  1:1730318/1‑97 (MQ=255)            cGATTGCAGGTTGGCGGGCAGGTGCAGCATCGCGGCCAGATTACTCATGGGTCGGCTTCTCGTTAATAAAATCCAGGGCTTCCTGGGCCGGAAgccctgg                                                                                        <  1:2085131/100‑7 (MQ=255)             gATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGTGCCGGAAgccctgga                                                                                       <  1:730290/100‑8 (MQ=255)             gATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGTGCCGGAAgccctgga                                                                                       <  2:407045/100‑8 (MQ=255)             gATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTa                                                                                       >  1:189405/1‑100 (MQ=255)               tttCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAgg                                                                                     <  2:632032/100‑1 (MQ=255)                 tCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCCCCGGAAGATGAGTAGGt                                                                                    >  1:2120914/1‑99 (MQ=255)                  cAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTg                                                                                  >  1:1952668/1‑100 (MQ=255)                  cAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTg                                                                                  <  2:1198417/100‑1 (MQ=255)                  cAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTg                                                                                  >  2:606990/1‑100 (MQ=255)                    ggTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTa                                                                                <  2:623871/100‑1 (MQ=255)                      tttGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACa                                                                              >  2:704332/1‑100 (MQ=255)                           gggCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGgcg                                                                         >  2:1655220/1‑100 (MQ=255)                            ggCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGgcgc                                                                        >  1:598274/1‑100 (MQ=255)                            ggCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTGCCGGCACCGGAAGATGAGTAGGTTGTACAGGgcgc                                                                        <  1:1167260/100‑1 (MQ=255)                               aGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGt                                                                     <  1:2526812/100‑1 (MQ=255)                               aGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGt                                                                     <  2:408575/100‑1 (MQ=255)                               aGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGt                                                                     <  1:630047/100‑1 (MQ=255)                                  cgcAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTg                                                                      <  2:132194/96‑1 (MQ=255)                                    cagcaTCGCGGCCAGATTACGCATTGGTTGGTTTGTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTccacc                                                                <  1:190797/100‑1 (MQ=255)                                     agcaTCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTccacca                                                               >  2:107573/1‑100 (MQ=255)                                        aTCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCAccagcc                                                            >  2:2111948/1‑100 (MQ=255)                                        aTCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCAccagcc                                                            <  2:678915/100‑1 (MQ=255)                                        aTCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCAccagcc                                                            <  2:643526/100‑1 (MQ=255)                                          cgcgGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCAccagccag                                                          >  2:1104095/1‑100 (MQ=255)                                          cgcgGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGGTGTACCGGGCGCTGTCCAccagccag                                                          >  1:1129424/1‑100 (MQ=255)                                            cgGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGAc                                                        >  2:461547/1‑100 (MQ=255)                                             ggCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACg                                                       <  2:1311518/100‑1 (MQ=255)                                             ggCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACg                                                       >  2:56671/1‑100 (MQ=255)                                              gCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGc                                                      >  1:572803/1‑100 (MQ=255)                                                       cTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTc                                             >  1:156603/1‑100 (MQ=255)                                                         cATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAg                                           >  1:775126/1‑100 (MQ=255)                                                         cATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAg                                           <  2:192354/100‑1 (MQ=255)                                                         cATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACAAGCCAGACGCCAGCGTTTCAg                                           >  1:1788540/1‑100 (MQ=255)                                                         cATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTCGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAg                                           >  1:494019/1‑100 (MQ=255)                                                         cATGGGTCGGTCTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAAGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAg                                           >  1:1361147/1‑100 (MQ=255)                                                            gggTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTg                                        <  2:754402/100‑1 (MQ=255)                                                             ggTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGc                                       >  1:489047/1‑100 (MQ=255)                                                              gTCGGTTTCTAGTTAATAAAATCCAGGGCTGCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGcc                                      <  1:2406423/100‑1 (MQ=255)                                                                cGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAg                                    <  1:177165/100‑1 (MQ=255)                                                                 ggTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGcc                                      >  1:2328626/1‑97 (MQ=255)                                                                   tttCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGAcc                                 <  1:73243/100‑1 (MQ=255)                                                                        cGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCATCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGc                            >  1:1358936/1‑100 (MQ=255)                                                                         gTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGcc                           <  2:2018854/100‑1 (MQ=255)                                                                         gTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGcc                           >  2:2294915/1‑100 (MQ=255)                                                                          ttAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCa                          <  1:2146223/100‑1 (MQ=255)                                                                            tataaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGc                        <  2:1239877/99‑1 (MQ=255)                                                                             ataaAATGCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCa                       <  1:1391768/100‑1 (MQ=255)                                                                             ataaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCa                       >  2:437903/1‑100 (MQ=255)                                                                             ataaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCa                       >  1:1239873/1‑100 (MQ=255)                                                                             ataaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCa                       <  1:960605/100‑1 (MQ=255)                                                                             ataaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCa                       >  2:530771/1‑100 (MQ=255)                                                                             ataaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCa                       >  2:2384405/1‑100 (MQ=255)                                                                             ataaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCa                       >  2:2099165/1‑100 (MQ=255)                                                                              taaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCAc                      >  2:1166076/1‑100 (MQ=255)                                                                              taaAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCAc                      <  2:28566/100‑1 (MQ=255)                                                                               aaaaTCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACt                     >  2:167027/1‑100 (MQ=255)                                                                                aaaTCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACtt                    >  1:971834/1‑100 (MQ=255)                                                                                 cctactcatcTTCCGGTGCCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTc                   >  2:1107704/10‑100 (MQ=255)                                                                                  ctactcatcTTCCGGTGCCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCCCCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTcc                  >  1:584338/9‑100 (MQ=255)                                                                                  aTCCTGGGCTTCCGGCACAGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCGAGACTCAGGCCAGCACTTcc                  >  1:2290311/1‑100 (MQ=255)                                                                                  aTCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTcc                  >  1:2248453/1‑100 (MQ=255)                                                                                   tCCATGGCTTCCGGCACCGGAAGATGAGTAGGGTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTcc                  <  1:108014/99‑1 (MQ=255)                                                                                   tCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCg                 <  2:475867/100‑1 (MQ=255)                                                                                    actcatcTTCCGGTGCCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCCCCAGCCAGACGCTAGCGTGTCAGTCGCCAGACCCTCGCCAGCACTTcc                  >  1:962463/7‑98 (MQ=255)                                                                                     cAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAAGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGcc               >  2:2259453/1‑100 (MQ=255)                                                                                       catcTTCCGGTGCCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCAc             >  2:1718855/4‑100 (MQ=255)                                                                                       catcTTCCGGTGCCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTCCCGCCAc             >  2:2293131/4‑100 (MQ=255)                                                                                        ggCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCAcc            >  1:1299380/1‑100 (MQ=255)                                                                                        ggCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCAcc            >  2:1765856/1‑100 (MQ=255)                                                                                        atcTTCCGGTGCCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCAcc            >  1:537220/3‑100 (MQ=255)                                                                                        atcTTCCGGTGCCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCAcc            >  2:1853370/3‑100 (MQ=255)                                                                                          cTTCCGGTGCCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACcgc          >  2:138722/1‑100 (MQ=255)                                                                                          cTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACcgc          >  1:1998877/1‑100 (MQ=255)                                                                                          cTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACcgc          <  2:561268/100‑1 (MQ=255)                                                                                          cTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACcgc          >  2:427433/1‑100 (MQ=255)                                                                                               ggCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGt     <  2:652519/100‑1 (MQ=255)                                                                                               ggCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGt     >  1:917074/1‑100 (MQ=255)                                                                                                gCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTa    >  1:1476987/1‑100 (MQ=255)                                                                                                  aCCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACa  >  1:2475548/1‑100 (MQ=255)                                                                                                  |                                                                                                    TGCCATTGTGTCGATTTCAGGTTTGCGGGCAGGCGCAGCATCGCGGCCAGATTACTCATGGGTCGGTTTCTCGTTAATAAAATCCAGGGCTTCCGGCACCGGAAGATGAGTAGGTTGTACAGGGCGCTGTCCACCAGCCAGACGCCAGCGTTTCAGTTGCCAGACCCAGGCCAGCACTTCCGCCACCGCGGCGTACA  >  NC_000913/1964992‑1965188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑