Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,440,991 A→G 81.5% G205G (GGT→GGC)  fabB ← 3‑oxoacyl‑[acyl‑carrier‑protein] synthase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,440,9910AG81.5% 242.5 / 43.1 113G205G (GGT→GGC) fabB3‑oxoacyl‑[acyl‑carrier‑protein] synthase I
Reads supporting (aligned to +/- strand):  ref base A (10/11);  new base G (43/49);  total (53/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01

Only 100 of 115 total aligned reads displayed.

ACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCG  >  NC_000913/2440893‑2441090
                                                                                                  |                                                                                                   
aCCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCAc                                                                                                    <  2:1797641/100‑1 (MQ=255)
 ccATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCATAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGcc                                                                                                   >  1:1433566/1‑100 (MQ=255)
   aTACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTCTGTCCGGGGTGTCGTGGTATTTAGTAGACAGCGCGccc                                                                                                  >  1:1674919/1‑99 (MQ=255)
    tACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCAt                                                                                                >  2:2024208/1‑100 (MQ=255)
       cgccgccgcCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGc                                                                                             <  2:1548307/100‑1 (MQ=255)
       cgccgccgcCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGc                                                                                             >  1:925282/1‑100 (MQ=255)
        gccgccgccAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGCGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCg                                                                                            >  2:1817655/1‑100 (MQ=255)
          cgccgccAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTc                                                                                          <  2:2158206/100‑1 (MQ=255)
           gccgccAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCAGTGCGTc                                                                                          <  1:891854/99‑1 (MQ=255)
              gccAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAAt                                                                                      >  2:482266/1‑100 (MQ=255)
                cAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAAtt                                                                                     <  1:282851/99‑1 (MQ=255)
                cAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTc                                                                                    <  2:488191/100‑1 (MQ=255)
                 aGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCg                                                                                   <  2:2485087/100‑1 (MQ=255)
                   cGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCa                                                                                 <  2:1883154/100‑1 (MQ=255)
                       aaCGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGcc                                                                             <  2:200223/100‑1 (MQ=255)
                        aCGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCa                                                                            <  2:1187214/100‑1 (MQ=255)
                        aCGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAAACAGCGCACCAATTGCGTCGAATTCGCAAGCCa                                                                            >  1:1542821/1‑100 (MQ=255)
                        aCGAAACCGTCACGGTGAGCGTCGTAAGTACGAGAGGCTTTTTCCGGGGAGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCa                                                                            >  1:1275995/1‑100 (MQ=255)
                         cGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCAt                                                                           <  2:1958626/100‑1 (MQ=255)
                          gAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCAtt                                                                          >  1:722675/1‑100 (MQ=255)
                          gAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCAtt                                                                          >  1:773407/1‑100 (MQ=255)
                           aaaCCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCAttt                                                                         >  1:226618/1‑100 (MQ=255)
                               cGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCa                                                                     <  2:1327118/100‑1 (MQ=255)
                                gTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAg                                                                    <  2:1245725/100‑1 (MQ=255)
                                gTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAg                                                                    <  2:1968038/100‑1 (MQ=255)
                                 tCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGc                                                                   >  1:111739/1‑100 (MQ=255)
                                 tCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGc                                                                   <  1:2460382/100‑1 (MQ=255)
                                 tCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGc                                                                   <  2:2007113/100‑1 (MQ=255)
                                 tCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGc                                                                   <  2:1813585/100‑1 (MQ=255)
                                  cACGGTGAGCGTCGTAAGTACGGGAGGCATTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGca                                                                  <  2:1245999/100‑1 (MQ=255)
                                   aCGGTGAGCGTCGTAAGTAGGGGAGGCTTTTTCCGGGGCGTCGTTGTATGTAGTAGACAGCGCGCCGATTGCGTCGAATTCGCAAGCCACTGCCGAGc                                                                   <  1:725429/98‑1 (MQ=255)
                                   aCGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGCATTCGCAAGCCATTTCCCAGcac                                                                 >  1:511822/1‑100 (MQ=255)
                                       tGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGct                                                             >  2:786407/1‑100 (MQ=255)
                                        gAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGctc                                                            <  2:2289174/100‑1 (MQ=255)
                                        gAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGctc                                                            <  2:722678/100‑1 (MQ=255)
                                        gAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGctc                                                            >  2:1435770/1‑100 (MQ=255)
                                            gtcgtAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTcg                                                        <  1:1248616/100‑1 (MQ=255)
                                            gtcgtAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTcg                                                        >  1:1893847/1‑100 (MQ=255)
                                             tcgtAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTcgc                                                       <  2:1019043/100‑1 (MQ=255)
                                              cgtAAGTACGGGAGGCTTTTTCCGGGGTGCCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGACATTTCCCAGCACAGCTCTTcgcc                                                      >  1:755165/1‑100 (MQ=255)
                                               gtAAGTACGGGCGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTcgccg                                                     <  1:1263028/100‑1 (MQ=255)
                                               gtAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTcgccg                                                     <  1:305790/100‑1 (MQ=255)
                                               gtAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTcgccg                                                     <  1:2066383/100‑1 (MQ=255)
                                                   gTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTcgccgccgc                                                 <  2:380004/100‑1 (MQ=255)
                                                    tACGGGAGGCTTTTTCCGGGGCGTCGTTGTATTTAGTAGACAGCGCGCCCATCTCGTCGAATGCGGACGCCATTTCCCAGCACACCTATGCgccgcagcc                                                >  1:2297393/1‑100 (MQ=255)
                                                      cGGGAGGCTTTTTCCGGGGTGTCGATGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAg                                              >  1:2111117/1‑100 (MQ=255)
                                                        ggAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCa                                            <  2:226620/100‑1 (MQ=255)
                                                         gAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCaa                                           >  1:836496/1‑100 (MQ=255)
                                                           ggCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAac                                         >  1:2465564/1‑100 (MQ=255)
                                                           ggCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAac                                         <  2:1341218/100‑1 (MQ=255)
                                                             cTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAacac                                       >  2:2489543/1‑100 (MQ=255)
                                                              tttttCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACg                                      <  2:644369/100‑1 (MQ=255)
                                                              tttttCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACg                                      <  1:281353/100‑1 (MQ=255)
                                                              tttttCCGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACg                                      >  1:1306218/1‑100 (MQ=255)
                                                                tttCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGAt                                    >  2:1468538/1‑100 (MQ=255)
                                                                   ccGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTc                                 <  2:1510046/100‑1 (MQ=255)
                                                                   ccGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTc                                 <  1:978129/100‑1 (MQ=255)
                                                                    cGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTcc                                <  2:1735143/100‑1 (MQ=255)
                                                                    cGGGGTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTAGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTcc                                >  2:177517/1‑100 (MQ=255)
                                                                     ggggTGTCGTTGTATTTAGTNGACAGCGCGCCCATTGCGTCGAATTCGCNAGCCATTTCCCAGCATAGCTCTTCGCCGNCCCNCGCAAACACGATGTCCt                               >  1:1363032/1‑100 (MQ=255)
                                                                     ggggTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCt                               >  2:190729/1‑100 (MQ=255)
                                                                     ggggTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCt                               >  2:1368416/1‑100 (MQ=255)
                                                                      gggTGTCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAacagatcggaagg                              >  2:2160668/1‑90 (MQ=255)
                                                                        gtgtCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGtt                            <  2:2398216/100‑1 (MQ=255)
                                                                        gtgtCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGtt                            <  1:1415384/100‑1 (MQ=255)
                                                                         tgtCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGGCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGttt                           <  1:419062/100‑1 (MQ=255)
                                                                          gtCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGttt                           <  1:418967/99‑1 (MQ=255)
                                                                           tCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGttt                           <  1:944784/98‑1 (MQ=255)
                                                                           tCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGc                         <  2:1569294/100‑1 (MQ=255)
                                                                           tCGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGc                         <  2:1123237/100‑1 (MQ=255)
                                                                            cGTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGcc                        >  2:1335003/1‑100 (MQ=255)
                                                                            cGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGcc                        >  2:1646674/1‑100 (MQ=255)
                                                                            cGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGcc                        <  2:1893857/100‑1 (MQ=255)
                                                                             gTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACAGGACATCGTGTTTGctg                       <  1:2390881/100‑3 (MQ=255)
                                                                             gTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGccc                       >  1:2155547/1‑100 (MQ=255)
                                                                             gTTGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGccc                       >  1:158609/1‑100 (MQ=255)
                                                                              ttGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCa                      <  1:1007002/100‑1 (MQ=255)
                                                                               tGTATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAg                     >  2:2186345/1‑100 (MQ=255)
                                                                                ctATTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGt                    <  2:247209/99‑1 (MQ=255)
                                                                                  aTTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGtt                   >  1:361741/1‑99 (MQ=255)
                                                                                  aTTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGtag                  >  1:247207/1‑98 (MQ=255)
                                                                                  aTTTAGTAGACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTg                  <  1:2489524/100‑1 (MQ=255)
                                                                                    ttAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGa                <  2:2533807/100‑1 (MQ=255)
                                                                                     tagtagACAGTGCGCCCATTGCGTCGCATTCGCACGCCATTTCCCAGCATATCTTTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTgta                <  1:1508427/99‑3 (MQ=255)
                                                                                      ngtagACAGCGCGCCCATTGCGTCGAATTCGCAGGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCCGTTGGATc              >  1:1278473/2‑100 (MQ=255)
                                                                                      agtagACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATc              <  1:1193885/100‑1 (MQ=255)
                                                                                      agtagACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATc              >  1:1918515/1‑100 (MQ=255)
                                                                                      agtagACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATc              >  2:1496540/1‑100 (MQ=255)
                                                                                      agtagACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATc              >  1:817875/1‑100 (MQ=255)
                                                                                       gtagACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATCt             >  1:2072750/1‑100 (MQ=255)
                                                                                         agACAGCGCGCCCATTGCGTCGAATTCGCAAGCAATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCCAACACGATGTCCTGTTTGCCCAGTTGGCTCTGc           >  1:1708193/1‑100 (MQ=255)
                                                                                         agACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTGGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATCTGc           <  1:1435762/100‑1 (MQ=255)
                                                                                          gACAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGAGGTCCTGTTTGCCCAGTTGGAgctgct          >  1:876045/1‑100 (MQ=255)
                                                                                          gACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATCTGct          <  1:602347/100‑1 (MQ=255)
                                                                                           aCAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATCTGctc         <  1:412260/100‑1 (MQ=255)
                                                                                            cAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATCTGctct        >  2:2183569/1‑100 (MQ=255)
                                                                                            cAGCGCGCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATCTGctct        <  2:1925640/100‑1 (MQ=255)
                                                                                              gcgcACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTAc      <  2:1547474/100‑1 (MQ=255)
                                                                                               cgcgCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCACACACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACt     >  1:976153/1‑100 (MQ=255)
                                                                                                  aCCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTgag  >  1:1567555/1‑98 (MQ=255)
                                                                                                  |                                                                                                   
ACCATACCGCCGCCGCCAGCGATAACGAAACCGTCACGGTGAGCGTCGTAAGTACGGGAGGCTTTTTCCGGGGTGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCG  >  NC_000913/2440893‑2441090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑