Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,474,878 T→C 16.9% intergenic (+22/‑106) yfdQ → / → yfdR CPS‑53 (KpLE1) prophage; uncharacterized protein/CPS‑53 (KpLE1) prophage; conserved protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,474,8780TC16.9% 233.2 / 37.0 106intergenic (+22/‑106)yfdQ/yfdRCPS‑53 (KpLE1) prophage; uncharacterized protein/CPS‑53 (KpLE1) prophage; conserved protein
Reads supporting (aligned to +/- strand):  ref base T (47/41);  new base C (12/6);  total (59/47)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

AACGAATTTCGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAG  >  NC_000913/2474779‑2474977
                                                                                                   |                                                                                                   
aaCGAATTTCGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCt                                                                                                     >  2:2450415/1‑100 (MQ=255)
aaCGAATTTCGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCt                                                                                                     >  1:130539/1‑100 (MQ=255)
 aCGAATTTCGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTg                                                                                                    >  1:159287/1‑100 (MQ=255)
     aTTTCGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCggg                                                                                                >  2:498178/1‑100 (MQ=255)
      tttCGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCgggg                                                                                               <  2:1907082/100‑1 (MQ=255)
       ttCGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCggggg                                                                                              <  2:143366/100‑1 (MQ=255)
        tCGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGc                                                                                             <  1:1408067/100‑1 (MQ=255)
         cGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCa                                                                                            <  2:277246/100‑1 (MQ=255)
             aTCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTa                                                                                        <  2:2502948/100‑1 (MQ=255)
             aTCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTa                                                                                        <  2:1067383/100‑1 (MQ=255)
              tCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCCGCAGGGGCAtttgc                                                                                       <  2:1083543/100‑3 (MQ=255)
                tGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCCGCAGGGGCAtttgcag                                                                                     <  1:1230867/100‑5 (MQ=255)
                tGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCCGCAGGGGCAtttgcag                                                                                     <  1:2358884/100‑5 (MQ=39)
                  cTTGTTAAGAAGTTCAAATACAGGAAGGTAGAAACCTTTACTGGTACTTTGTCCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATg                                                                                      <  1:2448598/97‑1 (MQ=255)
                   ttgttgAGAAATTCAAAGGCAGGAACGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCCGCAGTGTCAtttgcag                                                                                     <  1:1153622/97‑5 (MQ=255)
                    tgttgAGAAATTTAAAGACCACAAAGTAGAAAACTTTAATGGTACTTTCACCGGCTTGTTTCATTACTGCAAATGCCCCCGCGGGGGCATTTATGGAAAc                                                                                 <  1:1587927/100‑1 (MQ=255)
                     gttgAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACg                                                                                <  1:2504317/100‑1 (MQ=255)
                     gttgAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCCGCAGGGGCAtttgcagtaatg                                                                                <  2:1840788/100‑10 (MQ=38)
                          gaAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAAtt                                                                           >  2:211473/1‑100 (MQ=255)
                          gaAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAAtt                                                                           >  1:686640/1‑100 (MQ=255)
                          gaAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAAtt                                                                           >  2:1613877/1‑100 (MQ=255)
                           aaaTTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTa                                                                          >  2:1344224/1‑100 (MQ=255)
                             aTTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAAc                                                                        >  2:592264/1‑100 (MQ=255)
                              ttCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACt                                                                       >  2:1014309/1‑100 (MQ=255)
                              ttCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACt                                                                       >  1:1124550/1‑100 (MQ=255)
                                 aaaGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCaa                                                                    <  2:1549695/100‑1 (MQ=255)
                                   aGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCaata                                                                  >  2:335233/1‑100 (MQ=255)
                                         ggctgTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCAcc                                                            <  2:690120/96‑1 (MQ=255)
                                          aaaGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCg                                                           <  2:2134871/100‑1 (MQ=255)
                                            aGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGa                                                         <  1:789049/100‑1 (MQ=255)
                                             gTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGAt                                                        >  2:1568744/1‑100 (MQ=255)
                                              tAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATg                                                       >  1:2269148/1‑100 (MQ=255)
                                              tAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATg                                                       <  1:908962/100‑1 (MQ=255)
                                                gAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGt                                                     >  2:1215081/1‑100 (MQ=255)
                                                 aaaCCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTg                                                    <  2:1052261/100‑1 (MQ=255)
                                                  aaCCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGa                                                   >  2:2433704/1‑100 (MQ=255)
                                                     cTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGGCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAggg                                                >  1:984257/1‑100 (MQ=255)
                                                     cTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAggg                                                <  2:734036/100‑1 (MQ=255)
                                                      tttATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTGATGGAAACGTAATTAACTCAATAATCACCGGAGGGTGAGGGc                                               <  1:111492/100‑1 (MQ=255)
                                                      cgatctGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGc                                               <  1:1988493/95‑1 (MQ=255)
                                                         aTTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTc                                            >  2:1882413/1‑100 (MQ=255)
                                                           tGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCt                                          >  1:607875/1‑100 (MQ=255)
                                                           tGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCAGCCGGTGattattgagt                                          <  2:188224/100‑10 (MQ=255)
                                                            ggTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGcagatcg                                         >  2:1988504/1‑94 (MQ=255)
                                                            ggTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCtt                                         >  2:814587/1‑100 (MQ=255)
                                                              tACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCtttt                                       >  1:140362/1‑100 (MQ=255)
                                                               aCTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTa                                      <  1:1772727/100‑1 (MQ=255)
                                                                cTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTAc                                     >  1:710584/1‑100 (MQ=255)
                                                                cTTTCACCGCCTGATATCATTACTGCATTTTCCCCTTCTGGGGCATCTCTGGAATCGTAATTAACTCAATAATGAACGGGTGGTGAGGGCTTCCTTTTa                                      >  1:149714/1‑99 (MQ=255)
                                                                  ttCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTAccc                                   >  2:1142200/1‑100 (MQ=255)
                                                                   tCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCa                                  <  1:1181584/100‑1 (MQ=255)
                                                                      ccGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAAc                               <  1:231565/100‑1 (MQ=255)
                                                                      ccGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAAc                               <  2:159288/100‑1 (MQ=255)
                                                                       cGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACt                              <  1:227332/100‑1 (MQ=255)
                                                                              tttCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGcgcg                       >  2:2071586/1‑100 (MQ=255)
                                                                               ttCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCgg                      <  1:65436/100‑1 (MQ=255)
                                                                                  aTTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGc                   <  1:232180/100‑1 (MQ=255)
                                                                                   ttACTGCAAATGCCCATGCGGGGGCATTTATGGAAACGTAATTAACTCAATGGTCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCTGTGc                   >  1:2424581/1‑99 (MQ=255)
                                                                                    ttccataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAg                 >  2:1729069/7‑100 (MQ=38)
                                                                                    tACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAg                 >  2:1089612/1‑100 (MQ=255)
                                                                                    tACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAg                 <  2:1899608/100‑1 (MQ=255)
                                                                                     tccataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgc                >  2:1986551/6‑100 (MQ=39)
                                                                                     aCTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgc                <  2:1434827/100‑1 (MQ=255)
                                                                                      ccataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcg               >  1:32252/5‑100 (MQ=39)
                                                                                      cTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcg               >  1:709556/1‑100 (MQ=255)
                                                                                      cTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcg               <  2:2054336/100‑1 (MQ=255)
                                                                                      cTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcg               <  1:1142196/100‑1 (MQ=255)
                                                                                       tGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcgc              <  2:130540/100‑1 (MQ=255)
                                                                                       tGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcgc              >  2:1266209/1‑100 (MQ=255)
                                                                                       tGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcgc              <  2:1251668/100‑1 (MQ=255)
                                                                                       cataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGcttcgc              >  1:895604/4‑95 (MQ=255)
                                                                                       cataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcgc              >  2:814969/4‑100 (MQ=255)
                                                                                       cataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcgc              >  2:2528756/4‑100 (MQ=255)
                                                                                       cataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcgc              >  2:2590027/4‑100 (MQ=255)
                                                                                       cataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcgc              >  2:1979980/4‑100 (MQ=255)
                                                                                       cataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTAAATAATCACCGGATGGTGAGAGCTTCCTTTAACACAAACTCAGCGCGGTGCAgc                >  1:1498677/4‑98 (MQ=38)
                                                                                        gCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCa             >  1:1982220/1‑100 (MQ=255)
                                                                                        gCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCa             >  1:1087502/1‑100 (MQ=255)
                                                                                        gCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCa             >  1:1982206/1‑100 (MQ=255)
                                                                                        gCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCa             >  1:688858/1‑100 (MQ=255)
                                                                                        ataaaTGCCCCCGCAGGGGCATTTATGGCAACGTAATTAACTCAATAATCACCGGATGGTGAGAGCTTCATTTTACCCAAACTCAGCGCGGTGCAGCGCa             >  1:2202323/3‑100 (MQ=255)
                                                                                        ataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCa             >  2:1196321/3‑100 (MQ=255)
                                                                                         cAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTTACTCAATAATCGCCGGATGGTGAGGGATTCTTTTTACCAGAATTCAGCGCGGTGCAGCGCat            <  2:2124760/100‑1 (MQ=255)
                                                                                         cAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCTGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCat            <  2:1275494/100‑1 (MQ=255)
                                                                                         cAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCat            >  2:2167262/1‑100 (MQ=255)
                                                                                         cAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCat            <  2:1275858/100‑1 (MQ=255)
                                                                                          aaaTGCCCCTGCGGGGGCATTTATGGAAACGTAATTCACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCata           >  1:51724/1‑100 (MQ=255)
                                                                                          aaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCata           >  2:622439/1‑100 (MQ=255)
                                                                                           aaTGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCatat          <  1:523416/100‑1 (MQ=255)
                                                                                               ccccTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCTTATAc        >  1:2222377/1‑98 (MQ=255)
                                                                                               ccccTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGt      >  1:375190/1‑100 (MQ=255)
                                                                                               ccccTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGt      >  2:54398/1‑100 (MQ=255)
                                                                                               ccccTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGt      >  2:2524467/1‑100 (MQ=255)
                                                                                                cccTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTg     >  1:1971314/1‑100 (MQ=255)
                                                                                                cccTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTg     <  2:293118/100‑1 (MQ=255)
                                                                                                cccTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTg     >  2:282829/1‑100 (MQ=255)
                                                                                                cccTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTg     >  1:942347/1‑100 (MQ=255)
                                                                                                  cTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGga   <  1:1608910/100‑1 (MQ=255)
                                                                                                   tGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGgag  >  2:541535/1‑100 (MQ=255)
                                                                                                   tGCGGGGGCATGTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGgag  <  2:1280866/100‑1 (MQ=255)
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AACGAATTTCGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAG  >  NC_000913/2474779‑2474977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: