Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,474,881 G→A 16.1% intergenic (+25/‑103) yfdQ → / → yfdR CPS‑53 (KpLE1) prophage; uncharacterized protein/CPS‑53 (KpLE1) prophage; conserved protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,474,8810GA16.1% 244.9 / 33.1 105intergenic (+25/‑103)yfdQ/yfdRCPS‑53 (KpLE1) prophage; uncharacterized protein/CPS‑53 (KpLE1) prophage; conserved protein
Reads supporting (aligned to +/- strand):  ref base G (45/42);  new base A (12/5);  total (58/47)
Fisher's exact test for biased strand distribution p-value = 1.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01

Only 100 of 109 total aligned reads displayed.

ATTTCGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAAC  >  NC_000913/2474784‑2474980
                                                                                                 |                                                                                                   
aTTTCGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCggg                                                                                                   >  2:498178/1‑100 (MQ=255)
 tttCGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCgggg                                                                                                  <  2:1907082/100‑1 (MQ=255)
  ttCGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCggggg                                                                                                 <  2:143366/100‑1 (MQ=255)
   tCGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGc                                                                                                <  1:1408067/100‑1 (MQ=255)
    cGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCa                                                                                               <  2:277246/100‑1 (MQ=255)
        aTCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTa                                                                                           <  2:2502948/100‑1 (MQ=255)
        aTCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTa                                                                                           <  2:1067383/100‑1 (MQ=255)
        aTCTGCTGGTTGAGAAATTCAAAGACCGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATTCCCCTGCGGGGGCa                                                                                               <  1:1443072/96‑1 (MQ=255)
         tCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCCGCAGGGGCAtttgc                                                                                          <  2:1083543/100‑3 (MQ=255)
           tGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCCGCAGGGGCAtttgcag                                                                                        <  1:1230867/100‑5 (MQ=255)
           tGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCCGCAGGGGCAtttgcag                                                                                        <  1:2358884/100‑5 (MQ=39)
             cTTGTTAAGAAGTTCAAATACAGGAAGGTAGAAACCTTTACTGGTACTTTGTCCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATg                                                                                         <  1:2448598/97‑1 (MQ=255)
              ttgttgAGAAATTCAAAGGCAGGAACGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCCGCAGTGTCAtttgcag                                                                                        <  1:1153622/97‑5 (MQ=255)
                gttgAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACg                                                                                   <  1:2504317/100‑1 (MQ=255)
                gttgAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCCGCAGGGGCAtttgcagtaatg                                                                                   <  2:1840788/100‑10 (MQ=38)
                   gagaAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTaa                                                                                <  2:2141362/100‑1 (MQ=255)
                     gaAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAAtt                                                                              >  2:1613877/1‑100 (MQ=255)
                     gaAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAAtt                                                                              >  1:686640/1‑100 (MQ=255)
                     gaAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAAtt                                                                              >  2:211473/1‑100 (MQ=255)
                      aaaTTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTa                                                                             >  2:1344224/1‑100 (MQ=255)
                        aTTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAAc                                                                           >  2:592264/1‑100 (MQ=255)
                         ttCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACt                                                                          >  1:1124550/1‑100 (MQ=255)
                         ttCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACt                                                                          >  2:1014309/1‑100 (MQ=255)
                           cAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCa                                                                        >  2:1887080/1‑100 (MQ=255)
                            aaaGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCaa                                                                       <  2:1549695/100‑1 (MQ=255)
                              aGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCaata                                                                     >  2:335233/1‑100 (MQ=255)
                                   gCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCAc                                                                >  2:1385250/1‑100 (MQ=255)
                                    ggctgTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCAcc                                                               <  2:690120/96‑1 (MQ=255)
                                       aGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGa                                                            <  1:789049/100‑1 (MQ=255)
                                        gTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGAt                                                           >  2:1568744/1‑100 (MQ=255)
                                         tAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATg                                                          <  1:908962/100‑1 (MQ=255)
                                         tAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATg                                                          >  1:2269148/1‑100 (MQ=255)
                                           gAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGt                                                        >  2:1215081/1‑100 (MQ=255)
                                             aaCCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGa                                                      >  2:2433704/1‑100 (MQ=255)
                                                cTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGGCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAggg                                                   >  1:984257/1‑100 (MQ=255)
                                                cTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAggg                                                   <  2:734036/100‑1 (MQ=255)
                                                 tttATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTGATGGAAACGTAATTAACTCAATAATCACCGGAGGGTGAGGGc                                                  <  1:111492/100‑1 (MQ=255)
                                                 cgatctGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGc                                                  <  1:1988493/95‑1 (MQ=255)
                                                    aTTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTc                                               >  2:1882413/1‑100 (MQ=255)
                                                     ttGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTcc                                              <  2:1139924/100‑1 (MQ=255)
                                                      tGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCt                                             >  1:607875/1‑100 (MQ=255)
                                                       ggTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGcagatcg                                            >  2:1988504/1‑94 (MQ=255)
                                                       ggTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCtt                                            >  1:301723/1‑100 (MQ=255)
                                                       ggTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCtt                                            >  2:814587/1‑100 (MQ=255)
                                                         tACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCtttt                                          >  1:140362/1‑100 (MQ=255)
                                                          aCTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTa                                         <  1:1772727/100‑1 (MQ=255)
                                                           cTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTAc                                        >  1:710584/1‑100 (MQ=255)
                                                           cTTTCACCGCCTGATATCATTACTGCATTTTCCCCTTCTGGGGCATCTCTGGAATCGTAATTAACTCAATAATGAACGGGTGGTGAGGGCTTCCTTTTa                                         >  1:149714/1‑99 (MQ=255)
                                                             ttCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTAccc                                      >  2:1142200/1‑100 (MQ=255)
                                                              tCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCa                                     <  1:1181584/100‑1 (MQ=255)
                                                                 ccGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAAc                                  <  2:159288/100‑1 (MQ=255)
                                                                  cGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACt                                 <  1:227332/100‑1 (MQ=255)
                                                                         tttCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGcgcg                          >  2:2071586/1‑100 (MQ=255)
                                                                            cATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTg                       >  1:724158/1‑100 (MQ=255)
                                                                             aTTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGc                      <  1:232180/100‑1 (MQ=255)
                                                                              ttACTGCAAATGCCCATGCGGGGGCATTTATGGAAACGTAATTAACTCAATGGTCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCTGTGc                      >  1:2424581/1‑99 (MQ=255)
                                                                               ttccataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAg                    >  2:1729069/7‑100 (MQ=38)
                                                                               tACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAg                    >  2:1089612/1‑100 (MQ=255)
                                                                               tACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAg                    <  2:1899608/100‑1 (MQ=255)
                                                                                tccataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgc                   >  2:1986551/6‑100 (MQ=39)
                                                                                aCTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgc                   <  2:1434827/100‑1 (MQ=255)
                                                                                 ccataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcg                  >  1:32252/5‑100 (MQ=39)
                                                                                 cTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcg                  <  2:2054336/100‑1 (MQ=255)
                                                                                 cTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcg                  >  1:709556/1‑100 (MQ=255)
                                                                                 cTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcg                  <  1:1142196/100‑1 (MQ=255)
                                                                                  tGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcgc                 <  2:130540/100‑1 (MQ=255)
                                                                                  tGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcgc                 >  2:1266209/1‑100 (MQ=255)
                                                                                  tGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcgc                 <  2:1251668/100‑1 (MQ=255)
                                                                                  cataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGcttcgc                 >  1:895604/4‑95 (MQ=255)
                                                                                  cataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcgc                 >  2:2528756/4‑100 (MQ=255)
                                                                                  cataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcgc                 >  2:2590027/4‑100 (MQ=255)
                                                                                  cataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcgc                 >  2:814969/4‑100 (MQ=255)
                                                                                  cataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAgcgc                 >  2:1979980/4‑100 (MQ=255)
                                                                                  cataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTAAATAATCACCGGATGGTGAGAGCTTCCTTTAACACAAACTCAGCGCGGTGCAgc                   >  1:1498677/4‑98 (MQ=38)
                                                                                   gCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCa                >  1:1087502/1‑100 (MQ=255)
                                                                                   gCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCa                >  1:688858/1‑100 (MQ=255)
                                                                                   gCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCa                >  1:1982206/1‑100 (MQ=255)
                                                                                   gCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCa                >  1:1982220/1‑100 (MQ=255)
                                                                                   gCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCa                >  1:2183641/1‑100 (MQ=255)
                                                                                   ataaaTGCCCCCGCAGGGGCATTTATGGCAACGTAATTAACTCAATAATCACCGGATGGTGAGAGCTTCATTTTACCCAAACTCAGCGCGGTGCAGCGCa                >  1:2202323/3‑100 (MQ=255)
                                                                                   ataaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCa                >  2:1196321/3‑100 (MQ=255)
                                                                                    cAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTTACTCAATAATCGCCGGATGGTGAGGGATTCTTTTTACCAGAATTCAGCGCGGTGCAGCGCat               <  2:2124760/100‑1 (MQ=255)
                                                                                    cAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCTGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCat               <  2:1275494/100‑1 (MQ=255)
                                                                                    cAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCat               <  2:1275858/100‑1 (MQ=255)
                                                                                    cAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCat               >  2:2167262/1‑100 (MQ=255)
                                                                                     aaaTGCCCCTGCGGGGGCATTTATGGAAACGTAATTCACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCata              >  1:51724/1‑100 (MQ=255)
                                                                                     aaaTGCCCCCGCAGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCata              >  2:622439/1‑100 (MQ=255)
                                                                                      aaTGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCatat             <  1:523416/100‑1 (MQ=255)
                                                                                          ccccTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCTTATAc           >  1:2222377/1‑98 (MQ=255)
                                                                                          ccccTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGt         >  2:2524467/1‑100 (MQ=255)
                                                                                          ccccTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGt         >  1:375190/1‑100 (MQ=255)
                                                                                          ccccTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGt         >  2:54398/1‑100 (MQ=255)
                                                                                           cccTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTg        >  2:282829/1‑100 (MQ=255)
                                                                                           cccTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTg        >  1:1971314/1‑100 (MQ=255)
                                                                                           cccTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTg        >  1:942347/1‑100 (MQ=255)
                                                                                             cTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGga      <  2:301726/100‑1 (MQ=255)
                                                                                             cTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGga      <  1:1608910/100‑1 (MQ=255)
                                                                                              tGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGgag     >  2:541535/1‑100 (MQ=255)
                                                                                               gCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGgaga    <  2:2354684/100‑1 (MQ=255)
                                                                                                 gggggCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAAc  >  2:2182356/1‑100 (MQ=255)
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ATTTCGTGATCTGCTTGTTGAGAAATTCAAAGACAGCAAAGTAGAAACCTTTATTGGTACTTTCACCGCCTGATTTCATTACTGCAAATGCCCCTGCGGGGGCATTTATGGAAACGTAATTAACTCAATAATCACCGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAAC  >  NC_000913/2474784‑2474980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: