Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,636,291 A→C 15.9% L356V (TTG→GTG)  der ← GTPase; multicopy suppressor of ftsJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,636,2910AC15.9% 254.0 / 46.1 107L356V (TTG→GTG) derGTPase; multicopy suppressor of ftsJ
Reads supporting (aligned to +/- strand):  ref base A (47/43);  new base C (12/5);  total (59/48)
Fisher's exact test for biased strand distribution p-value = 1.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.20e-01

Only 100 of 108 total aligned reads displayed.

CATGATGCGCGTCAGCATAGAGGTCCCCACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCC  >  NC_000913/2636193‑2636389
                                                                                                  |                                                                                                  
cATGATGCGCGTCAGCATAGAGGTCCCCACACGACGGGTGGAGCTGTTATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAg                                                                                                   <  2:2519922/100‑1 (MQ=255)
  tgatgCGCGTCAGCATAGAGGTCCCCACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGc                                                                                                 <  2:874629/100‑1 (MQ=255)
   gatgCGCGTGCGCATAGAGGTCCCCACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAg                                                                                                   <  1:885191/97‑1 (MQ=255)
     tgCGCGTCAGCATAGAGGTCCCCACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCaga                                                                                              >  1:926483/1‑100 (MQ=255)
      gcgcGTCAGCATAGAGGTCCCCACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCTTGCACGGCagtg                                                                                             <  2:2356811/100‑3 (MQ=255)
      gcgcGTCAGCATAGAGGTCCCCACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCTTGCACGGCagt                                                                                              <  1:2391093/99‑2 (MQ=255)
       cgcgTCAGCATAGAGGTCCCCACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAgaga                                                                                            >  2:1140/1‑100 (MQ=255)
        gcgTCAGCATAGAGGTCCCCACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCTTTCACGGCagtggt                                                                                           <  2:1275856/100‑5 (MQ=255)
        gcgTCAGCATAGAGGTCCCCACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCTTGCACGGCagtggt                                                                                           <  2:1635019/100‑5 (MQ=255)
        gcgTCAGCATAGAGGTCCCCACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCTTGCACGGCagtggt                                                                                           <  1:2177472/100‑5 (MQ=255)
        gcgTCAGCATAGAGGTCCCCACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCATGCAAGGCAGAGAt                                                                                           <  1:800392/100‑1 (MQ=255)
           tCAGCATAGAGGTCCCCACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATaaa                                                                                        <  1:2432385/100‑1 (MQ=255)
           tCAGCATAGAGGTCCCCACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATaaa                                                                                        <  2:2354466/100‑1 (MQ=255)
             aGCATAGAGGTCCCCACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCagtggtgttgg                                                                                      >  2:2049559/1‑91 (MQ=255)
             aGCATAGAGGTCCCCACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGt                                                                                      >  1:782914/1‑100 (MQ=255)
              gCATAGAGGTCCCCACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTg                                                                                     >  2:2454608/1‑100 (MQ=255)
               cATAGAGGTCCCCACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGc                                                                                    <  2:849656/100‑1 (MQ=255)
               cATAGAGGTCCCCACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGc                                                                                    <  2:2500673/100‑1 (MQ=255)
                 tAGCGGTCCCCACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGca                                                                                   <  1:2295881/99‑1 (MQ=255)
                 tAGAGGTCCCCACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGcac                                                                                  >  1:2477486/1‑100 (MQ=255)
                   gagGTCCCCACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCacac                                                                                >  2:2227191/1‑100 (MQ=255)
                    agGTCCCCACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACg                                                                               <  2:65435/100‑1 (MQ=255)
                      gTCCCCACACGACGGGTGGAGCTGTCATACGCTTTACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAg                                                                             <  2:892542/100‑1 (MQ=255)
                      gTCCCCACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAg                                                                             <  2:700144/100‑1 (MQ=255)
                        ccccccACGACGGGCGTATCTGTCATACGCTTCACGTACTGATTCAAACAATTTGCGCACACCTCTGCCCGTCAAGGCAGCGATAATATGCacac                                                                                >  1:1803864/1‑95 (MQ=255)
                        ccccACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCa                                                                           >  2:1267675/1‑100 (MQ=255)
                          ccACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCaaa                                                                         <  1:255055/100‑1 (MQ=255)
                            acacGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAAt                                                                       >  2:701554/1‑100 (MQ=255)
                             cacGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATc                                                                      >  2:1080415/1‑100 (MQ=255)
                             cacGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATc                                                                      <  1:1822392/100‑1 (MQ=255)
                               cgacgGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGa                                                                    >  2:273053/1‑100 (MQ=255)
                                 acgGGTGGAGCTGTCATACGCTTCACGTACTGTTTCAAATAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATa                                                                  <  1:1819403/100‑1 (MQ=255)
                                 acgGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATa                                                                  <  2:1475773/100‑1 (MQ=255)
                                    ggTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAg                                                               >  2:797131/1‑100 (MQ=255)
                                    ggTGGACCTGTCATCCGCTTCACGTACTGATTCAAACAAGTGACCCACACCACTACCGTGCAAGCCAGAGATAAAGTACACACGAGCAAACTCGGTAAAg                                                               >  1:1749256/1‑100 (MQ=255)
                                     gTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGc                                                              <  1:1829808/100‑1 (MQ=255)
                                        gAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCa                                                           <  2:20361/100‑1 (MQ=255)
                                             gTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATACAGGGCACACGCGCAAAATCGATAAAGCCCAGACgg                                                      >  1:257136/1‑100 (MQ=255)
                                             gTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACgg                                                      >  1:2303040/1‑100 (MQ=255)
                                             gTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACgg                                                      >  2:1199206/1‑100 (MQ=255)
                                              tCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGa                                                     <  1:1029322/100‑1 (MQ=255)
                                                aTACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAg                                                   >  1:2031258/1‑100 (MQ=255)
                                                 tACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGt                                                  >  1:1108142/1‑100 (MQ=255)
                                                  aCGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTc                                                 <  2:1920945/100‑1 (MQ=255)
                                                   cGCTTCACGTACTGATTCAAACAAGTTACGAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTcc                                                <  1:1122702/100‑1 (MQ=255)
                                                     cTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAg                                              >  1:1315612/1‑100 (MQ=255)
                                                      ttCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGc                                             >  2:2050430/1‑100 (MQ=255)
                                                         aCGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGtt                                          >  2:1002113/1‑100 (MQ=255)
                                                            tACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGtttct                                       >  2:893476/1‑100 (MQ=255)
                                                            tACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGtttct                                       <  1:237698/100‑1 (MQ=255)
                                                             aCTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGtttctt                                      <  1:1119138/100‑1 (MQ=255)
                                                             aCTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGtttctt                                      <  2:1841409/100‑1 (MQ=255)
                                                             aCTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGtttctt                                      <  1:1879214/100‑1 (MQ=255)
                                                               tGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGtttctttc                                    >  2:1180004/1‑100 (MQ=255)
                                                               tGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGtttctttc                                    >  1:1103926/1‑100 (MQ=255)
                                                               tGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGtttctttc                                    <  2:1657714/100‑1 (MQ=255)
                                                               tGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGtttctttc                                    <  1:2527640/100‑1 (MQ=255)
                                                                gATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCa                                   >  2:2353088/1‑100 (MQ=255)
                                                                gATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCa                                   >  2:187088/1‑100 (MQ=255)
                                                                gATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCa                                   >  2:2574600/1‑100 (MQ=255)
                                                                 aTTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCAc                                  >  2:540098/1‑100 (MQ=255)
                                                                  ttCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCAcc                                 <  2:2031270/100‑1 (MQ=255)
                                                                   tCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCAcc                                 <  1:2336236/99‑1 (MQ=255)
                                                                   tCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCt                                >  1:2290229/1‑100 (MQ=255)
                                                                    cAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTg                               >  2:12700/1‑100 (MQ=255)
                                                                    cAAACAAGTTACCAACACCACTGCCGAGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACATg                               >  1:1004204/1‑100 (MQ=255)
                                                                     aaaCAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGc                              >  2:1117090/1‑100 (MQ=255)
                                                                      aaCAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGct                             >  2:1303414/1‑100 (MQ=255)
                                                                        cAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGctct                           >  2:1531821/1‑100 (MQ=255)
                                                                        cAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGctct                           >  1:1941763/1‑100 (MQ=255)
                                                                         aaGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCtt                          <  2:782917/100‑1 (MQ=255)
                                                                           gTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCttt                         <  2:2520981/99‑1 (MQ=255)
                                                                            ttACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCa                       <  1:2095507/100‑1 (MQ=255)
                                                                              cactttatctcTGCCTTGCACGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACt                     >  2:1655898/11‑100 (MQ=38)
                                                                              cactttatctcTGCCTTGCACGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACt                     >  1:432066/11‑100 (MQ=38)
                                                                               ccAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACtt                    >  2:50790/1‑100 (MQ=255)
                                                                               ccAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACtt                    >  1:1666016/1‑100 (MQ=255)
                                                                                ctttatctcTGCCTTGCACGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTc                   >  1:782476/9‑100 (MQ=255)
                                                                                ctttatctcTGCCTTGCACGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTc                   >  2:1910767/9‑100 (MQ=255)
                                                                                 aaCACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTcc                  <  2:843751/100‑1 (MQ=255)
                                                                                 aaCACCACTGCCGTGCAAGGCAGAGATAAAGTCCACCCAAGCAAACTCGATACAGCCCAGACGGAACCCCAGCGTATCTTTCACCTGCTCCTTCACTTcc                  >  1:776389/1‑100 (MQ=255)
                                                                                  acacCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTcc                  <  1:2013969/99‑1 (MQ=255)
                                                                                    atctcTGCCTTGCACGGCAGCGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGCTTCTTTCACCTGCCCTTTCACTTCCTg                >  1:195914/5‑99 (MQ=255)
                                                                                    atctcTGCCTTGCACGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTgt               >  1:1103236/5‑99 (MQ=255)
                                                                                    atctcTGCCTTGCACGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGa               >  1:862749/5‑100 (MQ=255)
                                                                                    atctcTGCCTTGCACGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGa               >  2:714616/5‑100 (MQ=255)
                                                                                    atctcTGCCTTGCACGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGa               >  2:1613215/5‑100 (MQ=255)
                                                                                     ccacTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGAc              >  2:1423209/1‑100 (MQ=255)
                                                                                      cacTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGCCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGAc              >  1:1745634/1‑99 (MQ=255)
                                                                                       tcTGCCTTGCACGGCAGAGATAAAGTGCACACGTGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTCCTTTCACCTGCTCTTTCACTTCCTGACTc            >  1:1561130/2‑100 (MQ=255)
                                                                                        cTGCCTTGCACGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCa           >  2:2101188/1‑100 (MQ=255)
                                                                                          gCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAgg         <  2:2213907/100‑1 (MQ=255)
                                                                                           ccGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGCCGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCTCTTCCTGACTCAGGc        <  2:2101256/100‑1 (MQ=255)
                                                                                           ccGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGc        <  2:478200/100‑1 (MQ=255)
                                                                                           ccGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGc        <  1:1672586/100‑1 (MQ=255)
                                                                                           ccGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGc        <  1:1861641/100‑1 (MQ=255)
                                                                                           ccGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGc        <  2:853945/100‑1 (MQ=255)
                                                                                              tGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCa      >  1:1855761/1‑99 (MQ=255)
                                                                                                cAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATcc   >  1:2406282/1‑100 (MQ=255)
                                                                                                cAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATcc   <  2:2477504/100‑1 (MQ=255)
                                                                                                  |                                                                                                  
CATGATGCGCGTCAGCATAGAGGTCCCCACACGACGGGTGGAGCTGTCATACGCTTCACGTACTGATTCAAACAAGTTACCAACACCACTGCCGTGCAAGGCAGAGATAAAGTGCACACGAGCAAAATCGATAAAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCC  >  NC_000913/2636193‑2636389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: