| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,800,515 | C→A | 26.2% | intergenic (+40/‑208) | ygaM → / → nrdH | putative membrane‑anchored DUF883 family ribosome‑binding protein/hydrogen donor for NrdEF electron transport system; glutaredoxin‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,800,515 | 0 | C | A | 26.2% | 129.5 / 45.9 | 74 | intergenic (+40/‑208) | ygaM/nrdH | putative membrane‑anchored DUF883 family ribosome‑binding protein/hydrogen donor for NrdEF electron transport system; glutaredoxin‑like protein |
| Reads supporting (aligned to +/- strand): ref base C (28/25); new base A (9/10); total (38/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.09e-01 | |||||||||||
ACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATT > NC_000913/2800419‑2800614 | aCAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTg < 2:446268/100‑1 (MQ=255)aCAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTg < 2:651574/100‑1 (MQ=255) cagcagCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGt > 2:402588/1‑100 (MQ=255) agcagcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTc > 1:697682/1‑100 (MQ=255) gcagcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCt > 1:282255/1‑100 (MQ=255) agcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTc > 2:1019698/1‑100 (MQ=255) gcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCg > 2:2483272/1‑100 (MQ=255) cTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCgg > 2:703750/1‑100 (MQ=255) tGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCggg < 2:2308365/100‑1 (MQ=255) tGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGACAGGCTTCggg < 1:495445/100‑1 (MQ=255) gCGGTAGGTATTTTTATCGGCGCACTGTTAAGCCGGCGGAAATTGTAGTGCAAAAATGGTAATAAATACGCAACTTTGACGCCGAAGCCTGTc > 1:661417/1‑93 (MQ=255) gCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCgggg > 2:1399416/1‑100 (MQ=255) gCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCgggg > 2:650420/1‑100 (MQ=255) gCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGACAGGCTTCgggg < 2:1642646/100‑1 (MQ=255) gCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGACAGGCTTCgggg < 1:260738/100‑1 (MQ=255) cGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGTAAAAATGAGAATAAGTACGCGTCTTTGACCCCGAAGGCTGTCTTCgggg > 2:1162323/1‑99 (MQ=255) cGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGt < 2:1505879/100‑1 (MQ=255) cGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGACAGGCTTCGGGGt < 1:1467126/100‑1 (MQ=255) cGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGACAGGCTTCGGGGt < 2:2208440/100‑1 (MQ=255) cGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGACAGGCTTCGGGGt < 2:2054867/100‑1 (MQ=255) cGGTAGGTATTTTCATCAGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTAACCCCGAAGACAGGCTTCGGGGt < 1:2014393/100‑1 (MQ=255) ggtaggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGtt < 1:2321418/100‑1 (MQ=255) ggtaggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGACAGGCTTCGGGGt < 1:1043947/99‑1 (MQ=255) gtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCttt > 2:1832878/1‑100 (MQ=255) gtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGACAGGCTTCGGGGtcaaaga < 2:1460267/100‑7 (MQ=38) taTTTTTATCGGCGCACTGTTAAGTATGCGCAAATCGTAGTGCAAACATGATAATAAATACGCGTCTTTGACCCCGAAGACAGGCTTCGGGGt < 1:1824601/93‑1 (MQ=255) taTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCtttt > 1:272964/1‑100 (MQ=255) aTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCttttt > 1:895970/1‑100 (MQ=255) aTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGAAAAAATGATAATAAATACGAGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCttttt > 1:1410589/1‑100 (MQ=255) tttttATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTg < 2:91532/100‑1 (MQ=255) tttttATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTg < 2:1059656/100‑1 (MQ=255) ttATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAATTGATAATAAATACGCGTCTTTGGCCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGcc < 2:282258/99‑1 (MQ=255) tATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATATTAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGcc < 1:2083809/98‑1 (MQ=255) aTCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTgg < 1:412542/100‑1 (MQ=255) ggCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTg < 1:1107721/99‑1 (MQ=255) ggCGCACTGATAAGCATGCGCAAATCGTAGTGGAAAACTGATAATAAATACGCGTCTTTGATCGCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGt > 1:61663/1‑98 (MQ=255) gcACTGTTAAGCATGAGCAAATCGTAGTGCAAAAATTATAATAAATACGCGTCTTTGACCGCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAAt < 1:2370225/99‑1 (MQ=255) cTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCTTGTTTTATcaca > 1:1281467/1‑100 (MQ=255) cTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATcaca > 2:2035759/1‑100 (MQ=255) gCATGCGCAAATCGTAGTGCAAAAATGATAATAAATAGGCGTCTTTGACCCCGAAGCCTGTCTTCTGGGTTTCTTTTTGCCTGGTGAATCACaaaa > 1:1384136/1‑96 (MQ=255) cATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGAGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCGGGTGAATCACAAAAATccc < 1:1282949/100‑1 (MQ=255) tGCGCAAATCGTAGTGCAAAAATGATAATAAATACGTGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATccccc < 2:697685/100‑1 (MQ=255) tGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATccccc > 2:584028/1‑100 (MQ=255) gcgcAAATCCTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAACAATccccc < 2:2162006/99‑1 (MQ=255) cgcAACTCGTAGTGCAAAAATGATAATAAATACAAGTCTTTGACCCCGAAACCTGTCTTCGGTGTTTCTTTTTGCCAGGTGAATCACAAAAATCACCCt > 1:181231/1‑99 (MQ=255) aaTCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTAcccc < 2:458867/100‑1 (MQ=255) gTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCa > 2:1206098/1‑100 (MQ=255) aatacAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGTCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACg < 1:1669101/96‑1 (MQ=255) tGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCt > 2:2062373/1‑100 (MQ=255) gCAAAAATGATAATAAATACGCGTCTTTGACCCCGACGCCTGTCTTCGGGGATTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTc > 1:452774/1‑100 (MQ=255) gCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTc > 1:1080515/1‑100 (MQ=255) gCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGAGGTTTCTTTTTGCCTGGTGAATCACACAAATCCCCCTACCCCGTCACGCTc > 1:2394027/1‑100 (MQ=255) aaaaTGATAATAAATACGCGTCTTTGACCCCGAAGCGTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCata < 2:1816733/100‑1 (MQ=255) aaaaTGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCata < 2:2054604/100‑1 (MQ=255) gATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAg < 1:2175682/100‑1 (MQ=255) gATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAg < 1:2435020/100‑1 (MQ=255) gATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAg < 2:353649/100‑1 (MQ=255) ataataAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAgg < 2:2482146/100‑1 (MQ=255) taataaATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAggg < 1:1758725/100‑1 (MQ=255) aataaataCGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGt > 1:857233/1‑100 (MQ=255) taaataCGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTaa > 2:350852/1‑100 (MQ=255) caaaaagaaaCCCCGAAGACAGGCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAc > 2:236381/10‑100 (MQ=37) cgTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTTAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCCGGGTAATTTCGAcc > 1:585850/1‑100 (MQ=255) gTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAcc < 2:2132413/99‑1 (MQ=255) aaCCCCGAAGACAGGCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAttt > 2:103316/2‑100 (MQ=255) aCCCCGAAGACAGGCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTg > 1:1615958/1‑100 (MQ=255) aCCCCGAAGACAGGCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTg > 2:1122268/1‑100 (MQ=255) aCCCCGAAGACAGGCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTg > 2:191290/1‑100 (MQ=255) aCCCCGAAGACAGGCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCCTCACGCTCATATCCCGGGTAATTTCGACCACTATTTg > 1:1743761/1‑100 (MQ=255) ccccGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGc < 2:2405909/100‑1 (MQ=255) ccccGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCAC‑AAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCt > 1:1497378/1‑100 (MQ=255) ccccGAAGACAGGCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGc > 2:146372/1‑100 (MQ=255) cccGAAGACAGGCTTCGGGGTTTCTTTTTGCCTTGTGAATCACAAAAATCCCCCTACCCCGTCACGATCATATCCAGGGTAATTTCGAACACTATTTCCt > 1:2071787/1‑100 (MQ=255) cccGAAGACAGGCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCt > 1:881504/1‑100 (MQ=255) ccTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATAtt > 2:321037/1‑100 (MQ=255) | ACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCTATATATT > NC_000913/2800419‑2800614 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |