Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 55,620 A→G 79.0% M497T (ATG→ACG)  lptD ← LPS assembly OM complex LptDE, beta‑barrel component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091355,6200AG79.0% 221.6 / 51.0 110M497T (ATG→ACG) lptDLPS assembly OM complex LptDE, beta‑barrel component
Reads supporting (aligned to +/- strand):  ref base A (12/11);  new base G (52/34);  total (64/46)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.91e-01

Only 100 of 111 total aligned reads displayed.

AGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACC  >  NC_000913/55522‑55716
                                                                                                  |                                                                                                
aGTCGTAGTTATAGATGTCGCTCTAACCGCGATACGGCACGTACAAATACTGCTCGCGCGGTTCCAGCGTTTGGGTATATCCCGGAGCCAGCATTTCCat                                                                                                 <  1:1578609/100‑1 (MQ=255)
   cGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATc                                                                                              >  1:2072669/1‑100 (MQ=255)
   cGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATc                                                                                              >  2:1858440/1‑100 (MQ=255)
    gTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATcg                                                                                             >  2:1704790/1‑100 (MQ=255)
     tAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATcac                                                                                            <  2:1754784/100‑3 (MQ=255)
      aGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATcgcg                                                                                           >  1:74791/1‑100 (MQ=255)
       gTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGt                                                                                          >  2:1044319/1‑100 (MQ=255)
       gTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGt                                                                                          <  2:2070049/100‑1 (MQ=255)
       gTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGt                                                                                          >  1:351821/1‑100 (MQ=255)
           taGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCaa                                                                                      <  1:2063691/100‑1 (MQ=255)
           taGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCaa                                                                                      >  1:2481765/1‑100 (MQ=255)
           taGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCaa                                                                                      >  1:1730479/1‑100 (MQ=255)
            aGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCaaa                                                                                     >  2:252564/1‑100 (MQ=255)
             gATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTCCAAAg                                                                                    <  1:2373854/100‑1 (MQ=255)
              aTGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGa                                                                                   >  2:1398572/1‑100 (MQ=255)
               tGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGAc                                                                                  >  2:2095786/1‑100 (MQ=255)
               tGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGAc                                                                                  >  1:1366413/1‑100 (MQ=255)
                 tCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCa                                                                                >  1:2499448/1‑100 (MQ=255)
                 tCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCa                                                                                >  1:730835/1‑100 (MQ=255)
                  cGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCAt                                                                               >  2:2329551/1‑100 (MQ=255)
                  cGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCAt                                                                               >  2:1587746/1‑100 (MQ=255)
                   gCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCAtt                                                                              >  1:194915/1‑100 (MQ=255)
                    ctctGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCAttt                                                                             >  1:1969029/1‑100 (MQ=255)
                    ctctGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCAttt                                                                             <  1:1220451/100‑1 (MQ=255)
                     tctGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCAtttt                                                                            <  2:1532610/100‑1 (MQ=255)
                        gATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGcc                                                                         >  2:2314593/1‑100 (MQ=255)
                        gATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGcc                                                                         >  1:2453705/1‑100 (MQ=255)
                        gATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGcc                                                                         >  2:1289385/1‑100 (MQ=255)
                        gATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGcc                                                                         >  2:1788036/1‑100 (MQ=255)
                         aTCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCg                                                                        <  2:2495610/100‑1 (MQ=255)
                          tCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGt                                                                       <  1:1463421/100‑1 (MQ=255)
                          tCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCg                                                                        <  1:1727751/99‑1 (MQ=255)
                             cgATACTTCATGTACAAATAAAGCGAGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACTATTTTTCCTTCaa                                                                    >  1:1196777/1‑100 (MQ=255)
                             cgATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCaa                                                                    >  2:826251/1‑100 (MQ=255)
                               aTACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACt                                                                  <  1:2095772/100‑1 (MQ=255)
                                tACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACtt                                                                 >  2:610696/1‑100 (MQ=255)
                                 aCGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCCACttt                                                                >  1:1136010/1‑100 (MQ=255)
                                  cGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACtgtg                                                               <  1:300479/100‑1 (MQ=255)
                                    gCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGaa                                                             >  1:531478/1‑100 (MQ=255)
                                     cACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAAt                                                            <  1:1659698/100‑1 (MQ=255)
                                       cGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTg                                                          >  2:86997/1‑100 (MQ=255)
                                       cGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTg                                                          >  2:2408008/1‑100 (MQ=255)
                                         tACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGCCCATTTTGTCGTCAACTTTGAATTGc                                                         >  2:1284742/1‑99 (MQ=255)
                                         tACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCg                                                        >  2:577856/1‑100 (MQ=255)
                                           cAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGc                                                      <  2:327486/100‑1 (MQ=255)
                                           cAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGc                                                      >  1:1086553/1‑100 (MQ=255)
                                             aaTACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCAt                                                    >  1:653574/1‑100 (MQ=255)
                                                 cTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTAcg                                                >  2:2481249/1‑100 (MQ=255)
                                                    cgcgcgcgGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCgg                                             <  2:627397/100‑1 (MQ=255)
                                                    cgcgcgcgGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCgg                                             <  2:1701402/100‑1 (MQ=255)
                                                       gcgcgGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTa                                          <  2:644261/100‑1 (MQ=255)
                                                        cgcgGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTaa                                         >  2:1547250/1‑100 (MQ=255)
                                                        cgcgGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTaa                                         >  2:2239072/1‑100 (MQ=255)
                                                          cgGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACg                                       <  2:2034584/100‑1 (MQ=255)
                                                           ggTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACgg                                      <  2:2042725/100‑1 (MQ=255)
                                                            gTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGCATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGGATTGCGGCATTACGCGGTTAACGGa                                     >  1:2201464/1‑100 (MQ=255)
                                                             ttCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGGCAACTTTGAATTGCGGCATTACGCGGTTAACGGAt                                    <  1:2251583/100‑1 (MQ=255)
                                                              tCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGAtt                                   <  1:1528740/100‑1 (MQ=255)
                                                                cAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCg                                 >  1:1685019/1‑100 (MQ=255)
                                                                cAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACAATTTGGCCGTCAACTTTGAATTGCGGAATTACGCGGTTAACGGATTCg                                 >  2:2364590/1‑100 (MQ=255)
                                                                cAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCg                                 >  1:798188/1‑100 (MQ=255)
                                                                cAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCg                                 <  2:1421464/100‑1 (MQ=255)
                                                                 aGCGTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCg                                 <  2:1132485/99‑1 (MQ=255)
                                                                    gTTTGGGTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCa                             <  1:2314579/100‑1 (MQ=255)
                                                                       tGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCt                          <  2:1136206/100‑1 (MQ=255)
                                                                        gggTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCtt                         >  2:1929229/1‑100 (MQ=255)
                                                                         ggTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTg                        >  2:1086133/1‑100 (MQ=255)
                                                                         ggTATAACCCGGAGCCAGCATTTCCGTATCGAGTTCAAAGACCATTTTGCCGTCATCTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCt                          <  1:1786148/98‑1 (MQ=255)
                                                                          ggataACCCTGAGGCAGCAGTTCCTTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTgg                       <  2:1119693/98‑1 (MQ=255)
                                                                          gTATAACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTgg                       >  1:1755587/1‑100 (MQ=255)
                                                                             taACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCg                    >  1:995251/1‑100 (MQ=255)
                                                                             taACCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCg                    <  1:826248/100‑1 (MQ=255)
                                                                             taACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCg                    <  1:975785/100‑1 (MQ=255)
                                                                             taACCCGGAGCCAGCATCTCCGTATCGCGGGCAAAGACCACCTCGCCGACATCTTTGAATTGCGGCATTACGGGGTTAACGGATTGTTCCAGCTTGGTc                     >  1:1990190/1‑99 (MQ=255)
                                                                             naaCCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCg                    >  1:904951/2‑100 (MQ=255)
                                                                              aaCCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCgt                   >  1:649296/1‑100 (MQ=255)
                                                                              aaCCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCgt                   >  2:1965186/1‑100 (MQ=255)
                                                                               aCCCGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTCCGCGGTTAACGGATTCGTCCAGCTTGGTCgtg                  <  2:1377534/100‑1 (MQ=255)
                                                                                cccGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCgtgt                 >  1:338458/1‑100 (MQ=255)
                                                                                 ccGGAGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGtt                <  2:851853/100‑1 (MQ=255)
                                                                                     aGCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTg            >  1:1373921/1‑100 (MQ=255)
                                                                                      gCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTgg           <  2:2362905/100‑1 (MQ=255)
                                                                                      gCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTgg           <  2:1086557/100‑1 (MQ=255)
                                                                                      gCCAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTg            >  2:292140/1‑99 (MQ=255)
                                                                                       ccAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGa          >  2:1726449/1‑100 (MQ=255)
                                                                                       ccAGCATTTCCGGATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCAGTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGa          <  1:287133/100‑1 (MQ=255)
                                                                                       ccAGCATGTCCGTATCGCGTTCAAAGACCACTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGa          <  2:1229636/100‑1 (MQ=255)
                                                                                        cAGCGTTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAAGTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGCCGTGTTTCTGGAg         <  1:335670/100‑1 (MQ=255)
                                                                                        cAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAg         >  2:2145633/1‑100 (MQ=255)
                                                                                        cAGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCACTCCGCCGTTAACGGATCCCTCCAGCCTGGCCGTCTTTc              >  1:408448/1‑95 (MQ=255)
                                                                                         atcATTTCCGTATAGCGTTCAAAGACCATTTTGCCGTCAACTGTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGt        >  2:1754573/3‑100 (MQ=255)
                                                                                         aGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAg         >  1:633923/1‑99 (MQ=255)
                                                                                         aGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGt        >  1:327201/1‑100 (MQ=255)
                                                                                         aGCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGt        <  1:67705/100‑1 (MQ=255)
                                                                                          gCATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGtt       >  2:182250/1‑100 (MQ=255)
                                                                                          gCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGtt       <  2:2379618/100‑1 (MQ=255)
                                                                                           cATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTta      >  2:2233559/1‑100 (MQ=255)
                                                                                           cATTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTta      <  2:1373929/100‑1 (MQ=255)
                                                                                           cATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTCCCGTCTTCGTCCAGCTTGGTCGTGTTTCTGGAGtt       >  1:2086987/1‑99 (MQ=255)
                                                                                            aTTTCCGTATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGt        <  1:1090158/97‑1 (MQ=255)
                                                                                                  |                                                                                                
AGTCGTAGTTATAGATGTCGCTCTGATCGCGATACGGCACGTACAAATACTGCGCGCGCGGTTCCAGCGTTTGGGTATAACCCGGAGCCAGCATTTCCATATCGCGTTCAAAGACCATTTTGCCGTCAACTTTGAATTGCGGCATTACGCGGTTAACGGATTCGTCCAGCTTGGTCGTGTTTCTGGAGTTATACC  >  NC_000913/55522‑55716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: