Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,171,858 A→T 19.2% intergenic (+25/+21) qseC → / ← ygiZ quorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,171,8580AT19.2% 264.1 / 54.2 114intergenic (+25/+21)qseC/ygiZquorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (40/52);  new base T (18/4);  total (58/56)
Fisher's exact test for biased strand distribution p-value = 1.64e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.87e-01

Only 100 of 125 total aligned reads displayed.

GCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACC  >  NC_000913/3171759‑3171957
                                                                                                   |                                                                                                   
gCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaa                                                                                                     >  2:1280274/1‑100 (MQ=255)
gCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaa                                                                                                     >  1:2549812/1‑100 (MQ=255)
gCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaa                                                                                                     >  2:541045/1‑100 (MQ=255)
gCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaa                                                                                                     >  1:2259071/1‑100 (MQ=255)
 ccAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaa                                                                                                    >  1:989196/1‑100 (MQ=255)
  cAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaaaa                                                                                                   <  1:1039625/100‑1 (MQ=255)
   aaaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAc                                                                                                  <  1:2256324/100‑1 (MQ=255)
      ttGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGAc                                                                                               <  2:1067256/100‑1 (MQ=255)
      ttGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGAc                                                                                               <  1:2052091/100‑1 (MQ=255)
       tGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGAGAAGGTAAGCTGGTAACTTCCCTGCCTATGACAGCCGTTAAACCGAc                                                                                               <  1:1677339/99‑1 (MQ=255)
        gcatggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGActc                                                                                             <  2:1298475/100‑1 (MQ=255)
           tggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCa                                                                                          <  1:601044/100‑1 (MQ=255)
             gcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATa                                                                                        <  2:1152649/100‑1 (MQ=255)
              catgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAg                                                                                       <  1:1556929/100‑1 (MQ=38)
                 gAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGaca                                                                                    <  1:738466/100‑3 (MQ=37)
                 gAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGAtt                                                                                    <  2:889770/100‑1 (MQ=255)
                 gAATGTTCAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGAtt                                                                                    <  2:2559750/100‑1 (MQ=255)
                   aTGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGAtttt                                                                                  <  1:1828661/100‑1 (MQ=255)
                    tGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGacagag                                                                                 >  1:2299739/1‑95 (MQ=25)
                     gTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTAc                                                                                >  2:691931/1‑100 (MQ=255)
                      ttGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGTTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTAc                                                                                <  1:1436935/99‑1 (MQ=255)
                      ttGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACt                                                                               <  2:1680983/100‑1 (MQ=255)
                      ttGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACt                                                                               >  2:143486/1‑100 (MQ=255)
                        gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTaa                                                                             >  2:1714781/1‑100 (MQ=255)
                        gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTaa                                                                             >  1:323057/1‑100 (MQ=255)
                        gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTaa                                                                             <  2:1389907/100‑1 (MQ=255)
                        gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTaa                                                                             <  2:2056904/100‑1 (MQ=255)
                          aTTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATa                                                                           >  2:761951/1‑100 (MQ=255)
                           tttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAg                                                                          <  2:2357602/100‑1 (MQ=255)
                           tttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAg                                                                          <  2:1283439/100‑1 (MQ=255)
                           tttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAg                                                                          <  2:740959/100‑1 (MQ=255)
                            ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGc                                                                         <  2:1098365/100‑1 (MQ=255)
                             tGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                        >  2:11129/1‑100 (MQ=255)
                               ggAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaaa                                                                      >  2:160510/1‑100 (MQ=255)
                               ggAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTGCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaaa                                                                      >  1:902251/1‑100 (MQ=255)
                                  aTGCGGAACAAGGTGGATTTGAGGCGAATGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAata                                                                   <  1:2031082/100‑1 (MQ=255)
                                  aTGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAata                                                                   >  2:1646536/1‑100 (MQ=255)
                                    cttccgatctgTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAAtata                                                                 <  2:2551942/90‑1 (MQ=255)
                                      gtaaCAAGGTGTATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATaaa                                                               <  1:1224029/98‑1 (MQ=255)
                                             ggTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTccc                                                        <  1:617367/100‑1 (MQ=255)
                                             ggTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATCAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTccc                                                        <  1:1652560/100‑1 (MQ=255)
                                               tGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCa                                                      <  2:1578498/100‑1 (MQ=255)
                                                ggATTTGAGGGCAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGAGTCTCATAGATTTTACTAATAGCAAAATATAAACCGTccc                                                        >  1:1175050/1‑97 (MQ=255)
                                                ggATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaa                                                     >  2:2403608/1‑100 (MQ=255)
                                                    ttGAGGCGAAGGTAATCTGGTAACTTCGCTGTCTATGATAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaaaa                                                 <  2:242003/100‑1 (MQ=255)
                                                     tGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAg                                                >  2:1499004/1‑100 (MQ=255)
                                                     tGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAg                                                <  1:1346261/100‑1 (MQ=255)
                                                     tGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAg                                                <  2:176807/100‑1 (MQ=255)
                                                        ggCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGcc                                              <  1:348638/99‑1 (MQ=255)
                                                        ggCGAAGGTAAGCGGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGcca                                             <  1:54908/100‑1 (MQ=255)
                                                         gCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGccac                                            <  2:1994406/100‑1 (MQ=255)
                                                          cGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGcca                                             >  2:1881320/1‑98 (MQ=255)
                                                             aGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaac                                        >  2:2126918/1‑100 (MQ=255)
                                                              ggTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaacc                                       <  1:688695/100‑1 (MQ=255)
                                                              ggTAAGCTGGTAACTTCGCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaacc                                       <  2:2327952/100‑1 (MQ=255)
                                                                tAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaacc                                       <  2:2122967/98‑1 (MQ=255)
                                                                tAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCAACCAACcac                                     >  1:2401589/1‑100 (MQ=255)
                                                                 aaGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcaca                                    <  1:1714772/100‑1 (MQ=255)
                                                                      ggTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCAACCAACCACAAAcaa                               >  1:2592202/1‑98 (MQ=255)
                                                                        tAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGa                             >  1:945469/1‑100 (MQ=255)
                                                                        tAACTTCTCTGTCTATGAGAGACGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACAGTAACCAAAAAAGCCACCAACCAAAAACCa                               >  1:1609837/1‑98 (MQ=255)
                                                                         aaCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGAc                            <  1:651854/100‑1 (MQ=255)
                                                                           cTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACaa                          >  2:120366/1‑100 (MQ=255)
                                                                           cTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACaa                          >  2:266342/1‑100 (MQ=255)
                                                                           cTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACaa                          >  1:1972305/1‑100 (MQ=255)
                                                                              ctctGTCTACGAGAGCCGTTAAAACGACTCTCATAAATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAGCCACAAACCAGACAAATa                       <  1:1801303/100‑1 (MQ=255)
                                                                               tctGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAc                      >  1:2501950/1‑100 (MQ=255)
                                                                                ctGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAc                      >  2:1202231/1‑99 (MQ=255)
                                                                                 aatCTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcg                    >  2:2355555/3‑100 (MQ=37)
                                                                                 aatCTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcg                    >  2:1968875/3‑100 (MQ=37)
                                                                                  gTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcga                   <  2:945472/100‑1 (MQ=255)
                                                                                  atCTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATTAACCGTACCCAAAAAAGCCACCAACCACAAAACAGACAAATACcga                   >  1:2492622/2‑100 (MQ=25)
                                                                                   tCTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACAAGACAAATACcga                   >  1:1407464/1‑99 (MQ=37)
                                                                                    cTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAcc                     >  1:1620318/1‑96 (MQ=38)
                                                                                    cTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc                 >  2:12123/1‑100 (MQ=38)
                                                                                    cTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc                 >  2:1309745/1‑100 (MQ=38)
                                                                                    cTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTACCCAAAAAAGCCACCAACCACAAACCAGACAAAAACcgagc                 >  2:1358713/1‑100 (MQ=37)
                                                                                    cTATGAGAGTCGGTTTAACGGCTCTTATAGATTTTACTAATATCAAAATATAAACCGTCGCCAAAACAGACACCAACGACAAACCAGTCAAATACcgagc                 >  1:1909313/1‑100 (MQ=255)
                                                                                    cTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc                 >  2:878749/1‑100 (MQ=255)
                                                                                    cTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc                 >  1:1556652/1‑100 (MQ=255)
                                                                                     tATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcg                >  1:614399/1‑100 (MQ=255)
                                                                                      aTGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTCATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcga               >  1:327997/1‑100 (MQ=38)
                                                                                      aTGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcga               >  2:2304010/1‑100 (MQ=38)
                                                                                      aTGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcga               >  1:947501/1‑100 (MQ=38)
                                                                                      aTGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcga               >  2:668225/1‑100 (MQ=38)
                                                                                      aTGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCAACAAACCACAAACAAGACAAATACcgaaaga               >  1:1641459/1‑96 (MQ=25)
                                                                                      aTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcga               <  2:614402/100‑1 (MQ=255)
                                                                                       tGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag              <  2:1372288/100‑1 (MQ=255)
                                                                                       tGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag              >  2:2019749/1‑100 (MQ=255)
                                                                                       tGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag              <  1:1948859/100‑1 (MQ=255)
                                                                                       tGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag              >  2:33888/1‑100 (MQ=255)
                                                                                          gagCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAAt           <  2:2194899/100‑1 (MQ=255)
                                                                                          gagCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCAAACGAGGAt           >  1:192796/1‑100 (MQ=255)
                                                                                            gCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATAc         <  2:1035026/100‑1 (MQ=255)
                                                                                              cGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACat       <  1:160509/100‑1 (MQ=255)
                                                                                               gTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACata      <  1:2112205/100‑1 (MQ=255)
                                                                                               gTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACata      >  2:908191/1‑100 (MQ=255)
                                                                                               gTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACata      >  1:939349/1‑100 (MQ=255)
                                                                                                  aaaaCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATAc   >  2:1327516/1‑100 (MQ=255)
                                                                                                   aaaCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATAc   >  1:1615372/1‑99 (MQ=255)
                                                                                                   |                                                                                                   
GCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACC  >  NC_000913/3171759‑3171957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: