Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,202,685 A→G 24.1% E265E (GAA→GAG)  cca → fused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,202,6850AG24.1% 190.4 / 68.7 116E265E (GAA→GAG) ccafused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase
Reads supporting (aligned to +/- strand):  ref base A (47/41);  new base G (13/15);  total (60/56)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.56e-01

Only 100 of 120 total aligned reads displayed.

CTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCC  >  NC_000913/3202586‑3202784
                                                                                                   |                                                                                                   
cTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGaa                                                                                                     >  2:2554376/1‑100 (MQ=255)
 ttAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAAc                                                                                                    <  2:2418406/100‑1 (MQ=255)
     tGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTg                                                                                                <  2:1670915/100‑1 (MQ=255)
     tGACGCTCTCAAGGGCGGCGATGCTGAGTCCGCAGGTCGATGGACGTTCCGCAACGGGATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTg                                                                                                <  1:2457756/100‑1 (MQ=255)
      gACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTgg                                                                                               >  1:2081156/1‑100 (MQ=255)
       aCGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGc                                                                                              <  2:636050/100‑1 (MQ=255)
       aCGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGc                                                                                              >  1:2361361/1‑100 (MQ=255)
        cGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGcc                                                                                             >  2:2241487/1‑100 (MQ=255)
          ctctCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTggcggc                                                                                           <  2:784570/100‑1 (MQ=255)
          ctctCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCcgc                                                                                           >  2:2956/1‑100 (MQ=255)
           tctcAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCcgcg                                                                                          >  2:412164/1‑100 (MQ=255)
             tcAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtc                                                                                        <  2:2522334/100‑1 (MQ=38)
                aTGGCGGCGATGTTGCGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                     <  2:1738054/100‑3 (MQ=37)
                aTGGCGGCGATGTTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                     <  1:13584/100‑3 (MQ=37)
                aTGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                     <  2:1223158/100‑3 (MQ=38)
                aTGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                     <  2:2392177/100‑3 (MQ=38)
                aTGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACGTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                     <  1:1977533/100‑3 (MQ=37)
                 tGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGtcatca                                                                                    >  2:1898860/1‑100 (MQ=255)
                  ggcgtggATGCTGCGCCCGCAGGGCGATTTCCGTCTCGAAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGgcagccc                                                                                   <  1:1877898/94‑5 (MQ=16)
                  ggcggcGATGCTGAGTCCGCAGGTCTATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                     <  1:1084185/98‑3 (MQ=37)
                  ggcggcGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTcatcat                                                                                   >  2:575501/1‑100 (MQ=255)
                  ggcggcGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTcatcat                                                                                   >  2:582507/1‑100 (MQ=255)
                  ggcggcGATGCTGAGTCCGCAGGTCGATGCCCGTTTCGACACTGTATGCCACGGTCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                     <  1:436538/98‑3 (MQ=25)
                  ggcggcGATGCGGAGTCCGCAGGGCGATGGCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagc                                                                                     <  1:1347682/98‑3 (MQ=25)
                   gcggcgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagccct                                                                                  <  2:2158312/100‑6 (MQ=37)
                   gcggcgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCGGCCAGAGTTCTGGCGGCGtcagccct                                                                                  <  2:2259170/100‑6 (MQ=37)
                   gcggcgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatg                                                                                  <  1:1342359/100‑1 (MQ=255)
                    cggcgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatgg                                                                                 <  2:2503811/100‑1 (MQ=255)
                    cggcgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatgg                                                                                 >  2:769425/1‑100 (MQ=255)
                    cggcgATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatgg                                                                                 >  2:1325245/1‑100 (MQ=255)
                    cggcgACGCTGAGTCCGCAGGTGGATGTCCGGGTCGCAACTTGATGCCTCGATCGCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatgg                                                                                 <  1:2496892/100‑1 (MQ=255)
                        gaggCTGAGTCCACAGGTCGATGTTAGTTTCACAACTTTAGCCCACGATCTCGCTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggt                                                                                <  1:603220/94‑1 (MQ=255)
                         aTGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAtcatggtcatg                                                                            >  1:577512/1‑100 (MQ=255)
                           gCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGc                                                                          >  2:1135843/1‑100 (MQ=255)
                            cTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGcc                                                                         <  2:1185817/100‑1 (MQ=255)
                            cTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGcc                                                                         >  2:2251008/1‑100 (MQ=255)
                            cTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGcc                                                                         <  1:41946/100‑1 (MQ=255)
                             tGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGccc                                                                        >  2:1366461/1‑100 (MQ=255)
                             tGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGcc                                                                         <  2:893263/99‑1 (MQ=255)
                              gAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGccag                                                                       >  1:1185813/1‑98 (MQ=255)
                              gAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCcg                                                                       <  2:921547/100‑1 (MQ=255)
                                   cGCAGGTAGATGTCCGTTTCGCAACTTTATGCCACGAACTCGGTAAAGGGCTGACGCCGGCAGAACTCTGGCCGGGTCATCCTGGTCATCGCCcggcg                                                                    >  1:1878839/1‑98 (MQ=255)
                                     cAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGgtg                                                                <  2:2169754/100‑1 (MQ=255)
                                       ggTCGATGTCCGTTTCGCAACTTTATGCCACTATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGgtgt                                                               <  1:1209230/99‑1 (MQ=255)
                                        gTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTa                                                             <  2:1616634/100‑1 (MQ=255)
                                        gTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTa                                                             <  1:1553480/100‑1 (MQ=255)
                                        gTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTa                                                             <  2:1793016/100‑1 (MQ=255)
                                        gTCGATGTCCGTTTCGCAACTTGATGCCCCGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGgtg                                                                <  1:900865/97‑1 (MQ=255)
                                          cGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTaa                                                            <  2:2491078/99‑1 (MQ=255)
                                              gTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAg                                                       >  1:1063849/1‑100 (MQ=255)
                                                ccGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTg                                                     <  2:1606981/100‑1 (MQ=255)
                                                    ttCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGaac                                                 >  1:1382065/1‑100 (MQ=255)
                                                      cGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGaacaa                                               >  2:1910552/1‑100 (MQ=255)
                                                       gCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCTGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGaacaac                                              <  2:790973/100‑1 (MQ=255)
                                                       gCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGcacaac                                              >  1:1446973/1‑100 (MQ=255)
                                                       gCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGaacaac                                              >  1:1938432/1‑100 (MQ=255)
                                                       gCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCAGCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGaacaac                                              <  2:1943725/100‑1 (MQ=255)
                                                           cTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATg                                          >  1:1011922/1‑100 (MQ=255)
                                                            tttATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGc                                         <  2:2305014/100‑1 (MQ=255)
                                                             ttATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGcc                                        >  1:2552981/1‑100 (MQ=255)
                                                             ttATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTATGGCCGCGTCATCATGGTCATGGCCCGGCGTGTGTTAAGTTAGTGGAACCACTATgac                                        >  1:1776156/1‑98 (MQ=255)
                                                                tGCCACGATCTGGGGCGAGGGCTGAGACAGCAAGAGCTCAGGCCGCGCAACCATGTTCTTTTCTTGTTGCGTGTTAAGTTAGTGGAACAACTATGCTAGc                                     >  1:2240427/1‑100 (MQ=255)
                                                                 gCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCg                                    >  2:1441315/1‑100 (MQ=255)
                                                                 gCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCg                                    <  1:590391/100‑1 (MQ=255)
                                                                 gCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCg                                    >  1:449478/1‑100 (MQ=255)
                                                                 gCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACGCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCg                                    >  2:1829828/1‑100 (MQ=255)
                                                                       aTCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTgcg                              <  1:1707475/100‑1 (MQ=255)
                                                                        tctcGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGGCTgcgc                             >  2:2118255/1‑100 (MQ=255)
                                                                         ctcGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGcgcg                            >  2:2268881/1‑100 (MQ=255)
                                                                             gTAAAGGGCTTACGCCGCCAGAACTGTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGcc                        <  1:1694372/100‑1 (MQ=255)
                                                                             gTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGcc                        >  2:1181540/1‑100 (MQ=255)
                                                                                aaGGGCTGACGCGGCCAGAACGCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGGTAAAGTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCan                      <  1:72479/99‑2 (MQ=255)
                                                                                 aGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAAt                    <  2:2054508/100‑1 (MQ=255)
                                                                                  atgatGACGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTTAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATg                   >  1:1232279/5‑100 (MQ=37)
                                                                                   ggCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGa                  >  1:2409555/1‑100 (MQ=255)
                                                                                    gCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGaa                 >  2:1302991/1‑100 (MQ=255)
                                                                                     atGACGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGaaa                >  2:1479325/2‑100 (MQ=38)
                                                                                      tGACGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAAt               >  2:745917/1‑100 (MQ=38)
                                                                                       gACGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAAtt              >  1:1166125/1‑100 (MQ=38)
                                                                                       gACGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAAtt              >  2:2080262/1‑100 (MQ=38)
                                                                                       gACGCGGCCAGAGTTCTGGCGGCGTCAGCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAAtt              >  2:2480842/1‑100 (MQ=37)
                                                                                       gACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAAtt              >  1:181264/1‑100 (MQ=255)
                                                                                       gACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAAtt              <  2:1542998/100‑1 (MQ=255)
                                                                                       gACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAAtt              >  2:2575987/1‑100 (MQ=255)
                                                                                       gACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGCTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAAtt              >  1:675349/1‑100 (MQ=255)
                                                                                        aCGCGGCCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTc             >  1:2326226/1‑100 (MQ=39)
                                                                                         cgcggcCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg            >  1:1331441/1‑100 (MQ=255)
                                                                                         cgcggcCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg            >  2:1516218/1‑100 (MQ=255)
                                                                                         cgcggcCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg            >  2:2169900/1‑100 (MQ=255)
                                                                                         cgcggcCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg            >  2:2163279/1‑100 (MQ=255)
                                                                                         cgcggcCAGAGTTCTGGCGGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg            >  2:1718602/1‑100 (MQ=255)
                                                                                         cgccgcCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg            >  1:1536289/1‑100 (MQ=255)
                                                                                         cgccgcCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg            >  1:122644/1‑100 (MQ=255)
                                                                                         cgccgcCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg            <  1:1080916/100‑1 (MQ=255)
                                                                                         cgccgcCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcg            >  1:736113/1‑100 (MQ=255)
                                                                                           ccgccAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTcgcg          <  1:2569930/100‑1 (MQ=255)
                                                                                             gccAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGAt        <  2:2428634/100‑1 (MQ=255)
                                                                                               cAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGAttt      >  1:522827/1‑100 (MQ=255)
                                                                                                  aaCTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGc   <  2:1063853/100‑1 (MQ=255)
                                                                                                   aCTCTGGCCGCGTCATCATGGTCATGGCCCGGCGTGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTa     <  1:1169905/97‑1 (MQ=255)
                                                                                                   |                                                                                                   
CTTAATGACGCTCTCAATGGCGGCGATGCTGAGTCCGCAGGTCGATGTCCGTTTCGCAACTTTATGCCACGATCTCGGTAAAGGGCTGACGCCGCCAGAACTCTGGCCGCGTCATCATGGTCATGGCCCGGCGGGTGTTAAGTTAGTGGAACAACTATGCCAGCGTCTGCGCGTGCCAAATGAAATTCGCGATTTAGCC  >  NC_000913/3202586‑3202784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑