Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,639,344 T→A 15.0% intergenic (+203/+41) pitA → / ← uspB phosphate transporter, low‑affinity; tellurite importer/universal stress (ethanol tolerance) protein B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,639,3440TA15.0% 299.8 / 36.7 113intergenic (+203/+41)pitA/uspBphosphate transporter, low‑affinity; tellurite importer/universal stress (ethanol tolerance) protein B
Reads supporting (aligned to +/- strand):  ref base T (51/45);  new base A (8/9);  total (59/54)
Fisher's exact test for biased strand distribution p-value = 7.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

Only 100 of 117 total aligned reads displayed.

AAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGG  >  NC_000913/3639245‑3639443
                                                                                                   |                                                                                                   
aaaCATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGttt                                                                                                     <  2:1502656/100‑1 (MQ=255)
 aaCATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTa                                                                                                    >  2:1625425/1‑100 (MQ=255)
   cATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACt                                                                                                  <  2:201709/100‑1 (MQ=255)
     tGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTgg                                                                                                <  2:2411822/100‑1 (MQ=255)
     tGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTgg                                                                                                >  2:2134199/1‑100 (MQ=255)
     tGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTgg                                                                                                <  2:1232849/100‑1 (MQ=255)
      gCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGc                                                                                               >  1:1040279/1‑100 (MQ=255)
          atgcAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGCCAGTAAACTGACCCGc                                                                                           <  1:2149323/97‑1 (MQ=21)
          aTTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGCCAGTAAACTGACCCGc                                                                                           <  1:996119/100‑1 (MQ=21)
          aTTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGCCAGTAAACTGACCCGc                                                                                           <  1:2227650/100‑1 (MQ=21)
          aTTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGCCAGTAAACTGACCCGc                                                                                           <  1:2183620/100‑1 (MQ=18)
           ttCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGCCAGTAAACTGACCCGCt                                                                                          <  1:420149/100‑1 (MQ=21)
            tCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGCCAGTAAACTGACCCGCtc                                                                                         <  2:2430671/100‑2 (MQ=21)
               aTAGATTGCAGAGATTATGTTGGCCTGATAAGCGTAGCGCATCAGGGAATTTTGCGTTTGTAATCAGGCTCGAGCGGGTCAGTTTACTGGCCGGCttttt                                                                                      >  1:258818/1‑100 (MQ=25)
               aTAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCttttt                                                                                      >  2:2569380/1‑100 (MQ=255)
                tAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGGATCAGGCAATTTTGCGTTTGCAATCAGTCTCGAGCGGGCCAGTAAACTGACCCGCtcga                                                                                       <  1:1597860/98‑4 (MQ=21)
                 aGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCttttttt                                                                                    <  2:1882738/100‑1 (MQ=255)
                  gATTGCAGAGATTATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTa                                                                                   >  2:1740977/1‑100 (MQ=255)
                   aTTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTAt                                                                                  >  1:2316555/1‑100 (MQ=255)
                     tGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGc                                                                                >  2:1986418/1‑100 (MQ=255)
                      gCAGAGATTATGTAGGCCTGATGAGCGTCGCGCATCAGGCAATTTTGCGTTTGTAATAAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCg                                                                               >  1:1154632/1‑100 (MQ=255)
                            aTTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGttatta                                                                         >  2:2018288/1‑100 (MQ=255)
                                 gTAGGCCTGATAAGCGTAGCGGATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCt                                                                    <  1:1638916/100‑1 (MQ=255)
                                 gTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCt                                                                    >  2:279181/1‑100 (MQ=255)
                                  tAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTg                                                                   >  1:315052/1‑100 (MQ=255)
                                  tAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTg                                                                   >  2:1342579/1‑100 (MQ=255)
                                   aGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGa                                                                  >  2:1031057/1‑100 (MQ=255)
                                    ggCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGaa                                                                 >  2:62758/1‑100 (MQ=255)
                                    ggCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGaa                                                                 <  1:1966844/100‑1 (MQ=255)
                                    ggCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGaa                                                                 >  1:1727684/1‑100 (MQ=255)
                                    ggCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGCTTGTAAGCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGaa                                                                 >  1:612995/1‑100 (MQ=255)
                                     gCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGATTACTGGCCCGTTTTTTTTATGCGTTATTATTg                                                                      >  2:2033860/1‑94 (MQ=255)
                                      ccTGATAGGCGTAGCGCATCAGGCAATTTTGCGTTTGTAACCACTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTATTATGCGCTATTATTGCTGCAGt                                                               >  1:1614799/1‑100 (MQ=37)
                                      ccTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGt                                                               <  1:2081035/100‑1 (MQ=255)
                                       cTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTg                                                              <  1:1342571/100‑1 (MQ=255)
                                       cTGATAAGCGTAGCGCATCAGGCAATTTTGCGCCTGTAATCAGACACGAGCAGGTCCGCTTCCTGGCCCGCTCTCTTTATGCGTTATTATTGCAGa                                                                  >  2:552916/1‑96 (MQ=21)
                                       cTGATAAGCGTAGCGCATCAGGCAATTCTGCGCCTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAg                                                                >  1:210924/1‑98 (MQ=255)
                                         gATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGc                                                             >  1:2219660/1‑99 (MQ=255)
                                          aTAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCtt                                                           <  2:1364837/100‑1 (MQ=255)
                                           tAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTa                                                          <  2:1690489/100‑1 (MQ=255)
                                            aaGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCTGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTaa                                                         <  1:1210778/100‑1 (MQ=255)
                                            aaGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTaa                                                         <  2:1889886/100‑1 (MQ=255)
                                             aGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAAt                                                        <  2:1727693/100‑1 (MQ=255)
                                             aGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAAt                                                        >  2:1316670/1‑100 (MQ=255)
                                              gCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGACCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATg                                                       <  1:1818784/100‑1 (MQ=255)
                                                  aGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTACTTAATGCCaa                                                   >  1:2261288/1‑100 (MQ=255)
                                                   gcgcATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCaaa                                                  <  1:1031054/100‑1 (MQ=255)
                                                    cgcATCAGCCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAat                                                 <  1:164952/100‑1 (MQ=255)
                                                     gcATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGTCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAatc                                                >  1:2191859/1‑100 (MQ=255)
                                                        tCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAatcatc                                             <  2:930015/100‑1 (MQ=255)
                                                          aGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCaa                                           >  2:1628794/1‑100 (MQ=255)
                                                           ggCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTTCTGAAGTGCTTAATGCCAAATCATCAAt                                          >  1:1061580/1‑100 (MQ=255)
                                                           ggCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAAt                                          >  1:1094522/1‑100 (MQ=255)
                                                           ggCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAAt                                          >  2:2023150/1‑100 (MQ=255)
                                                           ggCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAAt                                          <  2:310482/100‑1 (MQ=255)
                                                             cAATTTTGCGTTTGTAATCTGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGc                                        <  1:2406459/100‑1 (MQ=255)
                                                             cAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGc                                        <  1:2508199/100‑1 (MQ=255)
                                                             cAATTTTGCGTGTGTAATCAGTCTCGAGCGGGTCAGTTTACAGGCCCGCTTTTTTTATGCGATATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGc                                        >  1:1410763/1‑100 (MQ=255)
                                                              aaTTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCa                                       <  1:12496/100‑1 (MQ=255)
                                                              aaTTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCa                                       <  1:1066477/100‑1 (MQ=255)
                                                                 tttGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATc                                    <  1:1421595/100‑1 (MQ=255)
                                                                     cGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGc                                <  1:1328984/100‑1 (MQ=255)
                                                                      gTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCt                               >  2:1474424/1‑100 (MQ=255)
                                                                      gTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCt                               >  2:1705937/1‑100 (MQ=255)
                                                                        ttGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGa                             <  1:732165/100‑1 (MQ=255)
                                                                         tGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGac                            >  2:471869/1‑100 (MQ=255)
                                                                          gTAATCAGTCGCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGacc                           <  2:1290422/100‑1 (MQ=255)
                                                                           tAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGacca                          >  2:724779/1‑100 (MQ=255)
                                                                           tAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGacca                          >  2:2287420/1‑100 (MQ=255)
                                                                             aTCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGaccacc                        <  2:1499526/100‑1 (MQ=255)
                                                                             aTCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGaccacc                        <  1:2133330/100‑1 (MQ=255)
                                                                              cataaaaaaaGCGGGCCAGTAAACTGACCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGaccacca                       >  2:953857/10‑100 (MQ=25)
                                                                               ataaaaaaaGCGGGCCAGTAAACTGACCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGaccaccac                      >  1:2433538/9‑100 (MQ=37)
                                                                                aGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGaccaccacc                     >  2:1249223/1‑100 (MQ=255)
                                                                                aGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGaccaccacc                     <  1:1952841/100‑1 (MQ=255)
                                                                                aGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGaccaccacc                     <  1:2287405/100‑1 (MQ=255)
                                                                                 aaaaaaaGCGGGCCAGTAAACTGACCCGCTTTTTTTATGCGGTATTATTGCTCAAGTGCTTAATGCCAAATCCTCAATGCAATCAGGCTGaccaccacc                     >  1:2409084/7‑99 (MQ=25)
                                                                                  aaaaaaGCGGGCCAGTAAACTGACCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAg                   >  2:939040/6‑100 (MQ=37)
                                                                                    tcGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTTAAGTGCTTAATACCAAATCATCATTGCAATCAGGCGGACTACTACCAAAc                 >  1:1764804/1‑100 (MQ=255)
                                                                                    aaaaGCGGGCCAGTAAACTGACCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGAc                 >  1:47236/4‑100 (MQ=38)
                                                                                     cGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGAcc                <  2:2240925/100‑1 (MQ=255)
                                                                                     cGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGAcc                <  2:255657/100‑1 (MQ=255)
                                                                                     cGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGAcc                <  2:1619282/100‑1 (MQ=255)
                                                                                      aaGCGGGCCAGTAAACTGACCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGAc                 >  1:1569544/2‑98 (MQ=38)
                                                                                       aGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACcac              <  2:2046945/100‑1 (MQ=255)
                                                                                       aGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACcac              <  1:696846/100‑1 (MQ=255)
                                                                                       aGCGGGCCAGTAAACTGACCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACcac              >  2:592955/1‑100 (MQ=255)
                                                                                       aGCGGGCCAGTAAACTGACCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACcac              <  1:939037/100‑1 (MQ=255)
                                                                                        gCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACGAGACcaca             >  2:220568/1‑100 (MQ=255)
                                                                                        gCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACcaca             >  2:915385/1‑100 (MQ=255)
                                                                                        gCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACcaca             >  2:2246946/1‑100 (MQ=255)
                                                                                           ggTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAGGTGCTTAGTGTCACATTATCAATGCAATCAGGCTGACCACGGCCAGACcacaca           >  1:619368/1‑99 (MQ=255)
                                                                                           ggTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACaa          >  1:2378578/1‑100 (MQ=255)
                                                                                             tCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATg        <  1:1715406/100‑1 (MQ=255)
                                                                                             tCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGCCGCGCCCCCACCAGACCACACAAt         <  1:2126597/99‑1 (MQ=255)
                                                                                               aGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATgcg      >  2:564576/1‑100 (MQ=255)
                                                                                                gTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATgcgc     >  2:2293865/1‑100 (MQ=255)
                                                                                                  ttACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTg   >  2:371708/1‑100 (MQ=255)
                                                                                                  ttACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTg   <  1:150710/100‑1 (MQ=255)
                                                                                                   tACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTgg  <  1:2507126/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
AAACATGCAAATTCAATAGATTGCAGAGATTATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTTGTAATCAGTCTCGAGCGGGTCAGTTTACTGGCCCGCTTTTTTTATGCGTTATTATTGCTGAAGTGCTTAATGCCAAATCATCAATGCAATCAGGCTGACCACCACCAGACCACACAATGCGCTGG  >  NC_000913/3639245‑3639443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: