Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,658,002 C→T 15.2% intergenic (+435/‑364) hdeD → / → gadE acid‑resistance membrane protein/gad regulon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,658,0020CT15.2% 322.7 / 41.9 132intergenic (+435/‑364)hdeD/gadEacid‑resistance membrane protein/gad regulon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (62/50);  new base T (12/8);  total (74/58)
Fisher's exact test for biased strand distribution p-value = 8.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.60e-01

Only 100 of 139 total aligned reads displayed.

GCATAAATATCCGTGTCTCCAGACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCAGAGATCACAAACTGGTTATTGA  >  NC_000913/3657903‑3658101
                                                                                                   |                                                                                                   
gCATAAATATCCGTGTCTCCAGACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATc                                                                                                     >  1:34766/1‑100 (MQ=255)
gCATAAATATCCGTGTCTCCAGACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATc                                                                                                     >  2:1484296/1‑100 (MQ=255)
gCATAAATATCCGTGTCTCCAGACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATc                                                                                                     >  2:346156/1‑100 (MQ=255)
gCATAAATATCCGTGTCTCCAGACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATc                                                                                                     >  2:333658/1‑100 (MQ=255)
 cATAAATATCCGTGTCTCCAGACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATcc                                                                                                    >  2:21261/1‑100 (MQ=255)
 cATAAATATCCGTGTCTCCAGACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATcc                                                                                                    >  2:497395/1‑100 (MQ=255)
  aTAAATATCCGTGTCTCCAGACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCa                                                                                                   >  2:1491219/1‑100 (MQ=255)
      atatCCGTGTCTCCAGACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTg                                                                                               <  1:508284/100‑1 (MQ=255)
         tCCGTGTCTCCAGACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCa                                                                                            >  2:599673/1‑100 (MQ=255)
          ccGTGTCTCCAGACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAg                                                                                           <  2:1022918/100‑1 (MQ=255)
           cGTGTCTCCAGACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATTGGATTGCCAGc                                                                                          <  2:1113698/100‑1 (MQ=255)
           cGTGTCTCCAGACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAg                                                                                           >  1:646155/1‑99 (MQ=255)
           cGTGTCTCCAGACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGc                                                                                          <  2:849069/100‑1 (MQ=255)
            gtgtTTCCAGACGCTATATGAAAACCTGAAGACATGAATGCTTTAGTTGCTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATTGGATTGCCAGc                                                                                          <  1:2040296/99‑1 (MQ=255)
             tgtCTCCAGACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCtt                                                                                        <  1:2069412/100‑1 (MQ=255)
              gtCTCCAGACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTa                                                                                       >  1:1836372/1‑100 (MQ=255)
                ctcCAGACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAg                                                                                     <  1:1301452/100‑1 (MQ=255)
                 tcCAGACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGt                                                                                    >  2:1850032/1‑100 (MQ=255)
                 tcCAGACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGt                                                                                    >  2:1290819/1‑100 (MQ=255)
                  ccAGACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTc                                                                                   >  2:2327518/1‑100 (MQ=255)
                   cAGACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATTGGATTGCCAGCtttctttt                                                                                  <  1:724350/100‑7 (MQ=38)
                   cAGACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCg                                                                                  >  2:1500611/1‑100 (MQ=255)
                    aGACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATTGGATTGCCAGCtttctttta                                                                                 <  2:1233948/100‑8 (MQ=37)
                     gACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGaa                                                                                >  2:2594908/1‑100 (MQ=255)
                     gACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGaa                                                                                <  2:1651492/100‑1 (MQ=255)
                         cTATATAAAAACCTGAAGACATGAATACGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACaa                                                                            >  1:943715/1‑100 (MQ=255)
                          tatataAAAACCTGAAGACATGAATGCGTTATTTACTCAGTTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAg                                                                           <  1:469893/100‑1 (MQ=255)
                          tatataAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAg                                                                           >  1:635494/1‑100 (MQ=255)
                          tatataAAAACCTGAAGACATGAATACGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATTGGATTGCCAGCtttcttttaac                                                                               <  1:1079578/96‑10 (MQ=25)
                            tataAAAACCTGAAGATATGAATACGTTATTTACTCAGGTAATTTAAATGCGTTAAAAGAAAGCTGGAAATCCAATTGCCAGCTTAAGTCGAAACAAGga                                                                         <  1:2251035/100‑1 (MQ=255)
                            tataAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGga                                                                         <  2:117700/100‑1 (MQ=255)
                             ataAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGgag                                                                        >  1:2372856/1‑100 (MQ=255)
                             ataAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGgag                                                                        >  1:1937495/1‑100 (MQ=255)
                             ataAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGgag                                                                        <  2:1693065/100‑1 (MQ=255)
                              taAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGgaga                                                                       >  2:1328082/1‑100 (MQ=255)
                                  aaCCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCg                                                                   <  1:1024183/100‑1 (MQ=255)
                                    ccTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGat                                                                 <  2:2158959/100‑1 (MQ=255)
                                    ccTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGat                                                                 <  2:2354476/100‑1 (MQ=255)
                                     cTGAAGACATGAATGCGTTATTTACTCAGGTAATTTTAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGata                                                                >  1:1079969/1‑100 (MQ=255)
                                      tGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGatat                                                               <  2:34767/100‑1 (MQ=255)
                                       gAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATAtt                                                              <  2:2331044/100‑1 (MQ=255)
                                        aaGACATGAATGCGTTCTTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATAttt                                                             <  1:1513427/100‑1 (MQ=255)
                                        aaGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATAttt                                                             >  2:751166/1‑100 (MQ=255)
                                         aGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTa                                                            <  2:8039/100‑1 (MQ=255)
                                            cATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAAt                                                         >  2:2442447/1‑100 (MQ=255)
                                            cATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAAt                                                         >  1:139452/1‑100 (MQ=255)
                                             aTGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATc                                                        <  2:662103/100‑1 (MQ=255)
                                              tGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCg                                                       >  2:266649/1‑100 (MQ=255)
                                              tGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCg                                                       >  2:929255/1‑100 (MQ=255)
                                               gAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCTAGCTTAAGTCGAAACAAGGACACTCGATATTTAAATCgg                                                      <  1:1948581/100‑1 (MQ=255)
                                               gAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCgg                                                      <  1:660158/100‑1 (MQ=255)
                                                  tGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGAtt                                                   >  1:1334969/1‑100 (MQ=255)
                                                   gCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTa                                                  >  1:2300640/1‑100 (MQ=255)
                                                   gCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTa                                                  >  2:1946105/1‑100 (MQ=255)
                                                     gTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACa                                                >  2:2116757/1‑100 (MQ=255)
                                                        aTTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACAttt                                             <  2:1015747/100‑1 (MQ=255)
                                                         tttACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACAtttt                                            <  1:997672/100‑1 (MQ=255)
                                                           tACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTaa                                          >  2:43599/1‑100 (MQ=255)
                                                           tACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTaa                                          >  1:649634/1‑100 (MQ=255)
                                                            aCTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAAc                                         <  2:2216240/100‑1 (MQ=255)
                                                               cAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACttt                                      >  2:766146/1‑100 (MQ=255)
                                                               cAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACttt                                      >  1:2480864/1‑100 (MQ=255)
                                                                aGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACtt                                       >  1:2487388/1‑98 (MQ=255)
                                                                 ggTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAg                                    <  1:1000384/100‑1 (MQ=255)
                                                                 ggTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAg                                    >  1:889816/1‑100 (MQ=255)
                                                                  gTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGt                                   >  2:599972/1‑100 (MQ=255)
                                                                   tAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTa                                  >  1:1786252/1‑100 (MQ=255)
                                                                      tttCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTACCGTTAGTAata                               >  1:1937633/1‑100 (MQ=255)
                                                                      tttCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTaag                                >  1:1568063/1‑98 (MQ=255)
                                                                      tttCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAata                               >  2:696516/1‑100 (MQ=255)
                                                                        tCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATAtt                             >  1:2224905/1‑100 (MQ=255)
                                                                         cAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATAttc                            <  2:2370532/100‑1 (MQ=255)
                                                                            tGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCTAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATAttcttc                         <  2:1509932/100‑1 (MQ=255)
                                                                            tGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATAttcttc                         <  2:139453/100‑1 (MQ=255)
                                                                              ctttAATAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGCAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCag                       <  1:1250768/98‑1 (MQ=255)
                                                                               gTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCaga                      >  1:374026/1‑100 (MQ=255)
                                                                               gTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCaga                      >  2:1867551/1‑100 (MQ=255)
                                                                                ttAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCagag                     <  1:1460017/100‑1 (MQ=255)
                                                                                ttAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCagag                     >  1:1760717/1‑100 (MQ=255)
                                                                                  aaaaGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCAGAGAt                   >  1:1049882/1‑100 (MQ=255)
                                                                                   aaagaaagCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCAGAGATc                  <  2:1871388/100‑1 (MQ=255)
                                                                                     agaaagCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCAGAGATcac                >  2:2472958/1‑100 (MQ=255)
                                                                                     agaaagCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCAGAGATcac                >  2:242340/1‑100 (MQ=255)
                                                                                      gaaagCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCAGAGATcaca               >  2:2359486/1‑100 (MQ=255)
                                                                                      gaaagCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCAGAGATcaca               <  2:138940/100‑1 (MQ=255)
                                                                                      gaaagCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACGTTAGTAATATTCTTCAGAGATcaca               <  1:342768/100‑1 (MQ=255)
                                                                                         agCTGGCAATTGGATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCAGAGATCACAAAc            >  2:551728/1‑100 (MQ=255)
                                                                                         agCTGGCAATTGGATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCAGAGATCACAAAc            >  2:1591081/1‑100 (MQ=255)
                                                                                         agCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCAGAGATCACAAAc            <  2:1937505/100‑1 (MQ=255)
                                                                                          gCTGGCAATTGGATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCCGAGATCACAAACt           >  1:1271746/1‑100 (MQ=255)
                                                                                          gCTGGCAATTGGATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCAGAGATCACAAACt           >  1:1648726/1‑100 (MQ=255)
                                                                                          gCTGGCAATTGGATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCAGAGATCACAAACt           >  2:1455794/1‑100 (MQ=255)
                                                                                          gCTGGCAATTGGATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCAGAGATCACAAACt           >  1:155167/1‑100 (MQ=255)
                                                                                          gCTGGCAATTGGATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCAGAGATCACAAACt           >  1:1528520/1‑100 (MQ=255)
                                                                                          gCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCAGAGATCACAAACt           <  2:2053441/100‑1 (MQ=255)
                                                                                            tGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCAGAGATCACAAACTgg         <  1:235967/100‑1 (MQ=255)
                                                                                            tGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCAGAGATCACAAACTgg         <  2:1191624/100‑1 (MQ=255)
                                                                                               cAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCAGAGATCACAAACTGGTTa      >  2:1146122/1‑100 (MQ=255)
                                                                                                 aTCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCAGAGATCACAAACTGGTTAtt    >  2:1956843/1‑100 (MQ=255)
                                                                                                 aTCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCAGAGATCACAAACTGGTTAtt    <  1:798931/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
GCATAAATATCCGTGTCTCCAGACGCTATATAAAAACCTGAAGACATGAATGCGTTATTTACTCAGGTAATTTCAATGCGTTAAAAGAAAGCTGGCAATCCAATTGCCAGCTTAAGTCGAAACAAGGAGACTCGATATTTAAATCGGATTACATTTTAACTTTAGTAATATTCTTCAGAGATCACAAACTGGTTATTGA  >  NC_000913/3657903‑3658101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑