Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,696,226 C→T 18.9% intergenic (‑41/‑232) yhjR ← / → bcsE DUF2629 family protein/cellulose production protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,696,2260CT18.9% 229.6 / 44.7 106intergenic (‑41/‑232)yhjR/bcsEDUF2629 family protein/cellulose production protein
Reads supporting (aligned to +/- strand):  ref base C (48/38);  new base T (10/10);  total (58/48)
Fisher's exact test for biased strand distribution p-value = 8.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.21e-01

Only 100 of 108 total aligned reads displayed.

TTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAA  >  NC_000913/3696127‑3696323
                                                                                                   |                                                                                                 
ttCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGAc                                                                                                   <  2:1634266/100‑1 (MQ=255)
ttCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGAc                                                                                                   <  2:400615/100‑1 (MQ=255)
  cTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACaa                                                                                                 >  1:2425915/1‑100 (MQ=255)
   tGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGATAAt                                                                                                >  2:1854687/1‑100 (MQ=255)
    ggAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGATAAtt                                                                                               >  1:2096254/1‑100 (MQ=255)
    ggAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAAtt                                                                                               >  1:1789366/1‑100 (MQ=255)
    ggAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAAtt                                                                                               >  1:388907/1‑100 (MQ=255)
     gAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGATAATTc                                                                                              <  2:354462/100‑1 (MQ=255)
     gAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTc                                                                                              >  2:595990/1‑100 (MQ=255)
      aaGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCt                                                                                             <  1:1947015/100‑1 (MQ=255)
       aGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTa                                                                                            >  1:715640/1‑100 (MQ=255)
          tataGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAAcc                                                                                         <  1:1714238/100‑1 (MQ=255)
           ataGCCTATCGCGGGATCAGCCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCg                                                                                        >  2:2438409/1‑100 (MQ=255)
            taGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGt                                                                                       >  2:76526/1‑100 (MQ=255)
             gatcTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTg                                                                                      <  1:1160960/97‑1 (MQ=255)
             aGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGATAATTCTAACCGTg                                                                                      >  1:2227131/1‑100 (MQ=255)
             aGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTg                                                                                      >  2:1626771/1‑100 (MQ=255)
             aGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTg                                                                                      <  2:2078756/100‑1 (MQ=255)
              gCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGa                                                                                     >  2:195099/1‑100 (MQ=255)
               ccTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGATAATTCTAACCGTGAg                                                                                    >  2:1862107/1‑100 (MQ=255)
                  aTCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGATAATTCTAACCGTGAGCAt                                                                                 <  2:1626667/100‑1 (MQ=255)
                    cgcgGGATGAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTT                                                                               <  1:2224816‑M1/100‑1 (MQ=255)
                     gcgGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTC                                                                              <  2:59958‑M1/100‑1 (MQ=255)
                        ggATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGATAATTCTAACCGTGAGCATTTCGCC                                                                           >  2:1223304‑M1/1‑100 (MQ=255)
                        ggATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCC                                                                           <  2:729506‑M1/100‑1 (MQ=255)
                         gATCAGGCAGAGTATCTGGTTCATTGGTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCG                                                                          >  2:1580091‑M1/1‑100 (MQ=255)
                          aTCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCgg                                                                         >  2:1165099/1‑100 (MQ=255)
                          aTCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCgg                                                                         <  1:1147644/100‑1 (MQ=255)
                             aGGCAGAGTATCTGGTTCATTGTTATTCATTCTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGttt                                                                      <  1:485257/100‑1 (MQ=255)
                              ggCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGttt                                                                      >  1:1911852/1‑99 (MQ=255)
                                 agagTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTg                                                                  <  1:1575297/100‑1 (MQ=255)
                                 agagTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTg                                                                  <  2:2494416/100‑1 (MQ=255)
                                 agagTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTg                                                                  <  1:1902103/100‑1 (MQ=255)
                                 agagTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTg                                                                  <  1:1083101/100‑1 (MQ=255)
                                   agTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAACTAACCGTACCGTTCATGAGATAATTCTAACCGTTAGCATTTCGCCGGTTTATTGt                                                                 <  1:1507290/99‑1 (MQ=255)
                                   agTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGATAATTCTAACCGTGAGCATTTCGCCGGTTTATTGtt                                                                <  2:1303948/100‑1 (MQ=255)
                                   agTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACATTTCTAACCGTGAGCATTTCGCCGGTTTATTGtt                                                                >  1:1914491/1‑100 (MQ=255)
                                   agTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCATGAGCATTTCGCCGGTTTATTGtt                                                                >  1:1415311/1‑100 (MQ=255)
                                    gTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGttt                                                               <  1:76525/100‑1 (MQ=255)
                                    gTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGttt                                                               <  1:1029156/100‑1 (MQ=255)
                                      aTCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTAc                                                             >  2:1670081‑M2/1‑99 (MQ=255)
                                        cTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTAcgg                                                           >  2:847963‑M2/1‑97 (MQ=255)
                                         tGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTAcggc                                                          <  2:2542797‑M2/100‑5 (MQ=255)
                                          ggTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTAcggcg                                                         <  2:1042483‑M2/100‑6 (MQ=255)
                                            ttCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCgag                                                       >  2:698092/1‑100 (MQ=255)
                                             tCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGATAATTCTAACCGTGAGCATTTCGCCGtaaacaataaaccggcgaaa                                                      <  2:1863552‑M1/100‑21 (MQ=255)
                                             tCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGtaaacaataaaccggcgaaa                                                      >  1:291524‑M1/1‑80 (MQ=255)
                                             tCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGtaaacaataaaccggcgaaa                                                      >  1:106695‑M1/1‑80 (MQ=255)
                                               aTTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGATAATTCTAACCGTGAGCATTTCGCCGtaaacaataaaccggcgaaatg                                                    <  2:1911182‑M1/100‑23 (MQ=255)
                                               aTTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGtaaacaataaaccggcgaaatg                                                    <  2:1845662‑M1/100‑23 (MQ=255)
                                               aTTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGtaaacaataaaccggcgaaatg                                                    <  1:707295‑M1/100‑23 (MQ=255)
                                                ttGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGATAATTCTAACCGTGAGCATTTCGCCGtaaacaataaaccggcgaaatgc                                                   <  1:605767‑M1/100‑24 (MQ=255)
                                                ttGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGATAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACgg                                                   >  1:376989/1‑100 (MQ=255)
                                                ttGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGtaaacaataaaccggcgaaatgc                                                   <  2:291527‑M1/100‑24 (MQ=255)
                                                ttGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGtaaacaataaaccggcgaaatgc                                                   <  2:106696‑M1/100‑24 (MQ=255)
                                                ttGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGtaaacaataaaccggcgaaatg                                                    <  2:2562760‑M1/99‑23 (MQ=255)
                                                 tGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACggg                                                  >  2:1045377/1‑100 (MQ=255)
                                                     aTTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAAtt                                              >  2:841216/1‑100 (MQ=255)
                                                        cATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGc                                           <  1:2483494/100‑1 (MQ=255)
                                                        cATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGc                                           <  1:1057742/100‑1 (MQ=255)
                                                        cATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGc                                           >  2:1138176/1‑100 (MQ=255)
                                                         aTTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCt                                          >  1:1294912/1‑100 (MQ=255)
                                                          ttttTCTTTATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTc                                         >  1:1767527/1‑100 (MQ=255)
                                                                 tGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACttttt                                  <  1:1375135/100‑1 (MQ=255)
                                                                 tGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACttttt                                  >  1:2564793/1‑100 (MQ=255)
                                                                 tGATCCCTATGAAATAACCGTACCGCTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGTCGAGACGGGAATTTGCTCACttttt                                  >  1:339627/1‑100 (MQ=255)
                                                                  gATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTg                                 <  1:2108791/100‑1 (MQ=255)
                                                                    tCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGAc                               <  2:1001043/100‑1 (MQ=255)
                                                                    tCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGAc                               >  2:749142/1‑100 (MQ=255)
                                                                      ccTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGATTATAATATATAGCGAGACGGGAATTTGCTCACTGTTTGACtt                             >  1:1931391/1‑100 (MQ=255)
                                                                       cTATGAAATAACCGTACCGTTCATGAGATAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACttt                            >  2:1460965/1‑100 (MQ=255)
                                                                       cTATGAAATAACCGTACCGTTCATGAGATAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACttt                            >  2:1272579/1‑100 (MQ=255)
                                                                       cTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACttt                            >  1:1198475/1‑100 (MQ=255)
                                                                       cTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACttt                            >  2:2127293/1‑100 (MQ=255)
                                                                         aTGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTcc                          >  2:534440/1‑100 (MQ=255)
                                                                          tGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCg                         <  1:1940704/100‑1 (MQ=255)
                                                                           gAAATAACCGTACCGTTCATGAGATAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGt                        >  2:752170/1‑100 (MQ=255)
                                                                           gAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGt                        >  2:29738/1‑100 (MQ=255)
                                                                           gAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGt                        <  2:1808990/100‑1 (MQ=255)
                                                                            aaaTAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGtt                       >  1:2559399/1‑100 (MQ=255)
                                                                             aaTAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTa                      <  2:573669/100‑1 (MQ=255)
                                                                                  ccgtaccgtTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCaa                 >  2:1197066/1‑100 (MQ=255)
                                                                                     taccgtTCATGAGATAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAAc                <  1:1698045/98‑1 (MQ=255)
                                                                                     taccgtTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAg              <  2:1908398/100‑1 (MQ=255)
                                                                                       ccgtTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGaa            >  2:2283817/1‑100 (MQ=255)
                                                                                         gtTCATGAGATAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGaaaa          >  1:2496267/1‑100 (MQ=255)
                                                                                           tCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAg        <  1:43603/100‑1 (MQ=255)
                                                                                            cATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGt       >  2:1811126/1‑100 (MQ=255)
                                                                                            cATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGt       >  1:514201/1‑100 (MQ=255)
                                                                                              tGAGATAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTa     <  2:2227146/100‑1 (MQ=255)
                                                                                              tGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTa     <  1:2499966/100‑1 (MQ=255)
                                                                                              tGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTa     <  2:330331/100‑1 (MQ=255)
                                                                                               gagaCAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTaa    >  2:29908/1‑100 (MQ=255)
                                                                                                agaCAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTaaa   <  2:1248152/100‑1 (MQ=255)
                                                                                                agaCAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTaaa   >  2:1698989/1‑100 (MQ=255)
                                                                                                 gaCAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTaaaa  >  1:157462/1‑100 (MQ=255)
                                                                                                 gaCAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTaaaa  >  2:746405/1‑100 (MQ=255)
                                                                                                 gaCAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTaaaa  >  2:2015867/1‑100 (MQ=255)
                                                                                                 gaCAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTaaaa  >  1:313487/1‑100 (MQ=255)
                                                                                                 gaCAATTCTAACCGTGAACATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTaaaa  >  2:2565229/1‑100 (MQ=255)
                                                                                                   |                                                                                                 
TTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACCGTTCATGAGACAATTCTAACCGTGAGCATTTCGCCGGTTTATTGTTTACGGCGAGACGGGAATTTGCTCACTTTTTGACTTTCCGTTACGCAACAGAAAAAGTTAAAA  >  NC_000913/3696127‑3696323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: