Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,705,971 C→A 25.4% intergenic (‑181/+127) dppB ← / ← dppA dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9710CA25.4% 359.0 / 113.8 158intergenic (‑181/+127)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base C (72/45);  new base A (35/5);  total (107/51)
Fisher's exact test for biased strand distribution p-value = 2.81e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.20e-01

Only 100 of 170 total aligned reads displayed.

AGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCT  >  NC_000913/3705872‑3706070
                                                                                                   |                                                                                                        
aGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgc                                                                                                          <  2:691821/100‑1 (MQ=255)
   gaatGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcggc                                                                                                       >  1:78739‑M2/5‑100 (MQ=255)
    aatGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgcggcg                                                                                                      >  1:1540248‑M2/4‑100 (MQ=255)
       gAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTACGCATTGTTTATGCCGGATGCGGCGTTa                                                                                                   >  2:846295/1‑100 (MQ=255)
         aGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAAc                                                                                                 >  1:399549/1‑100 (MQ=255)
         aGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAAc                                                                                                 <  1:1051022/100‑1 (MQ=255)
             ttGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                                                                                             <  2:1593096/100‑1 (MQ=255)
              tgctgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCtt                                                                                            <  1:76062/100‑1 (MQ=255)
               gctgcCAGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                           >  1:958413/1‑100 (MQ=255)
                 tgcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATc                                                                                         >  1:105045/1‑100 (MQ=255)
                  gcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATcc                                                                                        >  2:1508511/1‑100 (MQ=255)
                    cGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCgg                                                                                      <  1:856062/100‑1 (MQ=255)
                    cGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCgg                                                                                      >  2:2467824/1‑100 (MQ=255)
                      gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                    <  2:753758/100‑1 (MQ=255)
                      gATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGca                                                                                    <  2:2328055/100‑2 (MQ=37)
                      gATGGCGCTGCACGCAGAGCCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGca                                                                                    <  1:2158910/100‑2 (MQ=25)
                         ggCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAc                                                                                 >  1:556861/1‑100 (MQ=255)
                         ggCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGCATaa                                                                                 <  1:654166/100‑2 (MQ=25)
                            gcTGCACTCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaa                                                                              >  2:1359750/1‑100 (MQ=255)
                            gcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaa                                                                              >  2:195091/1‑100 (MQ=255)
                                cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                          >  1:2378553/1‑100 (MQ=255)
                                cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                          <  1:1131245/100‑1 (MQ=255)
                                 aCGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                          <  1:222165/99‑1 (MQ=255)
                                 aCGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCAGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGt                                                                         >  1:2032969/1‑100 (MQ=255)
                                  cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCTGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                        <  2:1777335/100‑1 (MQ=255)
                                  cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                        >  1:816266/1‑100 (MQ=255)
                                  cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGAGTTAACGCCTTATCCGTCCTACAAAATCGt                                                                         >  1:1444376/1‑99 (MQ=255)
                                   gCAGAGGCACCGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGTCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                       <  1:1697668/100‑1 (MQ=255)
                                       agGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAt                                                                   <  1:1368188/100‑1 (MQ=255)
                                        ggCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt                                                                  >  2:1981337/1‑100 (MQ=255)
                                        ggCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt                                                                  >  1:460027/1‑100 (MQ=255)
                                            caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                              <  2:2123473/100‑1 (MQ=255)
                                            caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                              <  2:720621/100‑1 (MQ=255)
                                             aGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATa                                                             >  1:2265187/1‑100 (MQ=255)
                                             aGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATa                                                             <  1:2487073/100‑1 (MQ=255)
                                             aGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATAGTGCAAATTCAATa                                                             >  1:2526968/1‑100 (MQ=255)
                                              gcgcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATAc                                                            >  1:1446952/1‑100 (MQ=255)
                                                 ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                         >  2:1693903/1‑100 (MQ=255)
                                                 ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                         >  1:2062999/1‑100 (MQ=255)
                                                   aCCGGGTATACAGATTGTTGACAAATTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGc                                                       <  2:2435451/100‑1 (MQ=255)
                                                    ccGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCa                                                      >  2:2494180/1‑100 (MQ=255)
                                                     cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCa                                                      >  1:700297/1‑99 (MQ=255)
                                                     cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAg                                                    <  2:2485311/100‑1 (MQ=255)
                                                      gggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGa                                                   >  1:2417159/1‑100 (MQ=255)
                                                       ggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGaa                                                  >  2:1235673/1‑100 (MQ=255)
                                                        gTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAt                                                 >  1:348137/1‑100 (MQ=255)
                                                             cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑g                                        <  1:284366/100‑1 (MQ=255)
                                                              aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑Gt                                       >  1:1831980/1‑100 (MQ=255)
                                                              aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑Gt                                       >  1:1261948/1‑100 (MQ=255)
                                                                   gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGcc                                  >  2:758725/1‑100 (MQ=255)
                                                                   gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGcc                                  <  2:696685/100‑1 (MQ=255)
                                                                   gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGcc                                  >  2:2545212/1‑100 (MQ=255)
                                                                    ttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGc                                   <  1:755373/98‑1 (MQ=255)
                                                                     tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTg                                >  2:981493/1‑100 (MQ=255)
                                                                      gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGa                               >  1:2364354/1‑100 (MQ=255)
                                                                        cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATa                             <  2:1561342/100‑1 (MQ=255)
                                                                        cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATa                             >  2:161066/1‑100 (MQ=255)
                                                                           aGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGc                          >  1:1867536/1‑100 (MQ=255)
                                                                            gTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCg                         >  2:2369859/1‑100 (MQ=255)
                                                                            gTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCg                         >  2:2482500/1‑100 (MQ=255)
                                                                            gTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCg                         <  1:422691/100‑1 (MQ=255)
                                                                               cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAg                      <  1:225409/100‑1 (MQ=255)
                                                                                gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAgc                     >  2:1916458/1‑100 (MQ=255)
                                                                                gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAgc                     >  2:537295/1‑100 (MQ=255)
                                                                                 cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGACTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAgcg                    <  1:2384775/100‑1 (MQ=255)
                                                                                  cttttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGT‑‑TCAC‑‑GTAGGCCTGATAAGCGTAgcgc                   >  1:1474480/10‑100 (MQ=17)
                                                                                    tGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCAt                 >  2:2191555/1‑100 (MQ=255)
                                                                                    cttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAGTTCGTGCAAATTCAATAAATTGCAATA‑‑TGAC‑‑GTAGGCCTGATAAGCGTAGCGCATc                >  2:1622507/8‑100 (MQ=18)
                                                                                     ttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAAT‑‑TCTCTTGTAGGCCTGATAAGCGTAGCGCATc                >  1:1398748/7‑100 (MQ=18)
                                                                                     gTTTATGCCGGATGCAGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCa                  >  1:1030425/1‑98 (MQ=255)
                                                                                      tgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTACTTCAATATATTGCAGAAA‑‑CCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCa               >  2:2547540/6‑100 (MQ=17)
                                                                                       ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAg              <  1:548638/100‑1 (MQ=255)
                                                                                       ttATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAg              >  2:232625/1‑100 (MQ=255)
                                                                                       gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCATACAAAAGCGTGCAAATTCAATACATTGCATGGGC‑‑CAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg              >  2:2093804/5‑100 (MQ=17)
                                                                                       gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCATACAAAAGCGTGCAAATTCAATACATTGCATGGGC‑‑CAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg              >  2:2094488/5‑100 (MQ=17)
                                                                                       gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCGTACGAAATCGTGCTAATTCAATATATTGTGGGCT‑‑CCAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAg              >  2:260587/5‑100 (MQ=21)
                                                                                       gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAGTTCGTGCAAATTCAATAAATTGCAATA‑‑TGAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg             >  2:2300557/5‑100 (MQ=18)
                                                                                        taggCCGGATAAGGCGTTCACTCCGCATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGA‑‑TTGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg             >  2:325448/4‑100 (MQ=11)
                                                                                        taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTATAAAACTGTGCAAATTCAATATATTGCAGGAA‑‑ACAC‑‑GTAGGCCTGATAAGCGAAGCGCATCAgg             >  2:1828361/4‑100 (MQ=18)
                                                                                        taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGAT‑‑ACGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg             >  2:1006923/4‑100 (MQ=17)
                                                                                        tATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAgg             <  1:2011532/100‑1 (MQ=255)
                                                                                        tATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAgg             <  2:202421/100‑1 (MQ=255)
                                                                                        tATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAgg             >  2:530488/1‑100 (MQ=255)
                                                                                          ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAA‑‑CCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa           >  2:2544640/2‑100 (MQ=18)
                                                                                          ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAA‑‑CCTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa           >  2:639932/2‑100 (MQ=17)
                                                                                          ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTT‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGc            >  1:410447/2‑100 (MQ=21)
                                                                                          ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAA‑‑ATTC‑‑‑‑AGGCCTGATAAGCGTAGCGCATCAGGctt          >  1:1741140/2‑98 (MQ=25)
                                                                                          ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTT‑‑ATTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCa           >  2:133208/2‑100 (MQ=25)
                                                                                          ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGCAGTTT‑‑ATTG‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCa           >  2:352974/2‑100 (MQ=21)
                                                                                           gCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt          >  2:118933/1‑100 (MQ=255)
                                                                                           gCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGC‑‑CAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa          >  2:149501/1‑99 (MQ=17)
                                                                                           gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCAT‑‑CCTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa          >  1:1015799/1‑99 (MQ=21)
                                                                                           gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTGTGCAAATTCAATATATTGCAGGAA‑‑ACAC‑‑GTAGGCCTGATAAGCGAAGCGCATCAGGCag          >  2:422792/1‑99 (MQ=18)
                                                                                            ncGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAtt         >  1:695476/2‑100 (MQ=255)
                                                                                            ccGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATA‑‑TGAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGcgctt        >  1:1343535/1‑96 (MQ=18)
                                                                                             cGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAttt        <  2:1371691/100‑1 (MQ=255)
                                                                                                aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg     <  2:230715/100‑1 (MQ=255)
                                                                                                aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg     <  2:1090719/100‑1 (MQ=255)
                                                                                                  gcggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc   <  1:916956/100‑1 (MQ=255)
                                                                                                  gcggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc   <  1:1015627/100‑1 (MQ=255)
                                                                                                   |                                                                                                        
AGGGAATGAAGTATTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAT‑‑TTTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCT  >  NC_000913/3705872‑3706070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: