Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,705,984 T→G 34.0% intergenic (‑194/+114) dppB ← / ← dppA dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,9840TG34.0% 316.6 / 230.6 204intergenic (‑194/+114)dppB/dppAdipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base T (78/56);  new base G (64/5);  total (142/62)
Fisher's exact test for biased strand distribution p-value = 1.09e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-01

Only 100 of 207 total aligned reads displayed.

TTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGT  >  NC_000913/3705885‑3706081
                                                                                                   |                                                                                                          
  gcttcgggATGGGGCTGCGCGCGGGGGCCCACCGCTCCCGGGTCGACGGATTGTTGACAACGTGCGTTTTGTTTATGCCGGTTGCGGCGTTAACGCCTTa                                                                                                          <  1:2251500/94‑1 (MQ=12)
  gctgcCAGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                                                                                                          >  1:958413/1‑100 (MQ=255)
     gcCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATcc                                                                                                       >  2:1508511/1‑100 (MQ=255)
       cGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCgg                                                                                                     <  1:856062/100‑1 (MQ=255)
       cGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCgg                                                                                                     >  2:2132833/1‑100 (MQ=255)
         gATGGCGCTGCACGCAGAGCCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGca                                                                                                   <  1:2158910/100‑2 (MQ=25)
            ggCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAc                                                                                                >  1:556861/1‑100 (MQ=255)
            ggCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATAAGGCGTTAACGCCGCATCCGGCATaa                                                                                                <  1:654166/100‑2 (MQ=25)
              cgcTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaa                                                                                             >  2:703729/1‑100 (MQ=255)
               gcTGCACTCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaa                                                                                            >  2:1359750/1‑100 (MQ=255)
                cTGCACACAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaaa                                                                                           <  1:2177060/100‑1 (MQ=255)
                   cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                                        <  1:1130908/100‑1 (MQ=255)
                   cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                                        >  1:2378553/1‑100 (MQ=255)
                   cACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCg                                                                                        <  2:105046/100‑1 (MQ=255)
                     cGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCTGCGTTAACGCCTTATCCGGCCTACAAAATCGTg                                                                                      <  2:1777335/100‑1 (MQ=255)
                      gCAGAGGCACCGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGTCGTTAACGCCTTATCCGGCCTACAAAATCGTGc                                                                                     <  1:1697668/100‑1 (MQ=255)
                           ggCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt                                                                                >  1:460027/1‑100 (MQ=255)
                           ggCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt                                                                                >  2:1207103/1‑100 (MQ=255)
                            gCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc                                                                               <  2:1920824/100‑1 (MQ=255)
                               caGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAt                                                                            <  1:552189/100‑1 (MQ=255)
                                aGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATa                                                                           <  1:2487073/100‑1 (MQ=255)
                                aGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATa                                                                           >  1:2265187/1‑100 (MQ=255)
                                 gcgcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATAc                                                                          >  1:1446952/1‑100 (MQ=255)
                                   gcCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAt                                                                        >  2:2384083/1‑100 (MQ=255)
                                    ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                                       >  1:2062999/1‑100 (MQ=255)
                                    ccACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACAtt                                                                       >  2:1693903/1‑100 (MQ=255)
                                     cACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTg                                                                      >  2:2327539/1‑100 (MQ=255)
                                      aCCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGc                                                                     >  2:2451626/1‑100 (MQ=255)
                                       ccGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCa                                                                    >  2:2494180/1‑100 (MQ=255)
                                        cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCa                                                                    >  1:700297/1‑99 (MQ=255)
                                        cGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAg                                                                  <  2:2485311/100‑1 (MQ=255)
                                        cGGGTATACAGATTGTTGACAAAGTGCGCTTGGTTTATGCCGAATGCGGCGTTAACGCCTTATCCGGCCTACAAAATGGTGCAAATTCAATACATTGCAg                                                                  >  2:2325510/1‑100 (MQ=255)
                                         gggTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGa                                                                 >  1:2417159/1‑100 (MQ=255)
                                           gTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAt                                                              >  1:348137/1‑100 (MQ=255)
                                            tataCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAAtt                                                            >  2:336142/1‑100 (MQ=255)
                                                cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑g                                                   <  1:284366/100‑1 (MQ=255)
                                                 aGATTGTTGACCAAGTGCGCCTCGTTTATGCCCACTGCGGCGTTAACGCCCTATCCGGCCAACAAAATCGTGCAAATTAACAACATTGCAGAACT‑‑‑TTC‑‑Gt                                                  <  1:1024901/100‑1 (MQ=17)
                                                 aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAATGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑Gt                                                  >  1:1438554/1‑100 (MQ=255)
                                                 aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑Gt                                                  >  1:1831980/1‑100 (MQ=255)
                                                 aGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑Gt                                                  >  1:1261948/1‑100 (MQ=255)
                                                    ttgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAgg                                               >  1:809208/1‑100 (MQ=255)
                                                      gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGcc                                             >  2:2545212/1‑100 (MQ=255)
                                                      gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGcc                                             >  2:758725/1‑100 (MQ=255)
                                                      ataaaCAAAGCGCACTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCTGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGcc                                             >  1:117391/5‑100 (MQ=18)
                                                        tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTg                                           >  2:511919/1‑100 (MQ=255)
                                                         gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGa                                          >  2:1838328/1‑100 (MQ=255)
                                                         gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGa                                          >  1:2364354/1‑100 (MQ=255)
                                                          aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGAt                                         >  1:757542/1‑100 (MQ=255)
                                                           cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATa                                        >  2:742774/1‑100 (MQ=255)
                                                           cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATa                                        <  2:1561342/100‑1 (MQ=255)
                                                              aGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGc                                     >  1:672309/1‑100 (MQ=255)
                                                                  cgcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAg                                 >  1:416677/1‑100 (MQ=255)
                                                                   gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgc                                >  2:537295/1‑100 (MQ=255)
                                                                     cttttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAgcgc                              >  1:1474480/10‑100 (MQ=17)
                                                                     attttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCATACAAAATCGTGCTAATTCAATATATTGCAGAAAC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAgcgc                              >  2:2021847/10‑100 (MQ=17)
                                                                       tttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTGTGCAAATTCAATATATTGCAGGAAA‑‑‑CAC‑‑GTAGGCCTGATAAGCGAAGCGCAt                            >  2:815023/8‑100 (MQ=18)
                                                                       cttgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAGTTCGTGCAAATTCAATAAATTGCAATAT‑‑‑GAC‑‑GTAGGCCTGATAAGCGTAGCGCATc                           >  2:1622507/8‑100 (MQ=18)
                                                                        ttgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCATACAAAATCGTGCTAATTCAATATATTGCAGAAAC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATc                           >  1:13733/7‑100 (MQ=17)
                                                                         tgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAATCGTGCTACTTCAATATATTGCAGAAAC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCa                          >  2:2547540/6‑100 (MQ=17)
                                                                          gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCATACAAAATCGTGCTAATTCAATATATTGCAGAAAC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                         <  1:2021835/96‑1 (MQ=18)
                                                                          gtaggCCGGATAAGGCGTTTACGCCGCATCCGGCATACAAAAGCGTGCAAATTCAATACATTGCATGGGCC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                         >  2:2094488/5‑100 (MQ=17)
                                                                          gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCGTACGAAATCGTGCTAATTCAATATATTGTGGGCTC‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                         >  2:260587/5‑100 (MQ=21)
                                                                          gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCATACAAGTTCGTGCAAATTCAATAAATTGCAATAT‑‑‑GAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                        >  2:2300557/5‑100 (MQ=18)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGTTCGTGCAAATTCAATAAATTGCAATAT‑‑‑GAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGcg                      >  1:1032087/3‑99 (MQ=21)
                                                                            aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGT‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                      >  2:1601413/3‑100 (MQ=21)
                                                                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                      >  2:588614/2‑100 (MQ=21)
                                                                              gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAACTGTGCAAATTCAATATATTGCAGGAAA‑‑‑CAC‑‑GTAGGCCTGATAAGCGAAGCGCATCAGGCag                     >  2:422792/1‑99 (MQ=18)
                                                                               ccggataaggcgTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAttt                   >  2:1320515/9‑100 (MQ=18)
                                                                               ccggataaggcgTTCACGCCTTATCCGGCCTACAAAACTTTGCAAATTCAATATATTGCAATC‑‑‑TCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCaat                    >  2:2028248/9‑98 (MQ=18)
                                                                               ccggataaggcgTTCACGCCGCATCCGGCCTACAAGATCGTGCAAATTCAATATATTGCATGAGC‑‑‑CAC‑‑GTTGTCCTGATAATCGTAACTCATCAGACAtt                    >  2:1144440/9‑100 (MQ=12)
                                                                               ccggataaggcgTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaat                    >  2:955026/9‑98 (MQ=21)
                                                                               ccggataaggcgTTCACGCCGCATCCGGCCTACAAAATCGCTTAAATTCAATATATTGCACCATC‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaat                    >  2:2089857/9‑98 (MQ=21)
                                                                               ccggataaggcgTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGC‑‑‑TGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                    >  2:2097606/9‑98 (MQ=18)
                                                                               ccggataaggcgTTCACGCCGCATCCGGCCTACAAAAACCTGCAAATTCAATATATTGCAGGATA‑‑‑CGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaat                    >  2:360834/9‑98 (MQ=18)
                                                                               ccggataaggcgTTCACGCCGCATCCGGCATACAAAAACATGCAAATTCAATAGATTGCAGAGATT‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaat                    >  1:1986042/9‑98 (MQ=17)
                                                                                cGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAttt                   <  2:1371691/100‑1 (MQ=255)
                                                                                 ggataaggcgTTTACGCCGCATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtttgc                 >  2:1591717/7‑98 (MQ=21)
                                                                                 ggataaggcgTTTACGCCGCATCCGGCCTACAAAAGCATGCAAATTCAATATATTGCAGAGTT‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                  >  2:2275949/7‑100 (MQ=17)
                                                                                 ggataaggcgTTTACGCCGCATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTT‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaatt                   >  2:841306/7‑97 (MQ=21)
                                                                                 ggataaggcgTTTACGCCGCATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTT‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCaatt                   >  1:733678/7‑97 (MQ=21)
                                                                                 ggataaggcgTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGACC‑‑‑AT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                  >  2:1968722/7‑100 (MQ=18)
                                                                                 ggataaggcgTTCACGCAGCATCCGGCCTACAAATCATGCAAATCCAAAATATTGCAGGAGA‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAttta                  >  1:1213401/7‑98 (MQ=18)
                                                                                  gataaggcgTTTACGCCGCATCCGGCCTACAAAACAGTGCAAATTCAATATATTGCAATCTT‑‑‑CTG‑‑GTAGGCCTGATAAGCATCGCGCATCAGGCaagctt                 >  1:442042/6‑95 (MQ=21)
                                                                                  gataaggcgTTCACGCCGCATCCGGCCTACAAAATCATGAAAATTCAATACATTGCAAGATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCacgctc                 >  2:1935684/6‑95 (MQ=18)
                                                                                   aTGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg                <  1:2399014/100‑1 (MQ=255)
                                                                                     gcggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc              <  1:1015627/100‑1 (MQ=255)
                                                                                     aaggcgTTAACGCCGCATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcc              <  1:1933765/98‑1 (MQ=255)
                                                                                      cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCACTtttgcc              >  2:674919/1‑99 (MQ=255)
                                                                                       ggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctt            <  2:1644513/100‑1 (MQ=255)
                                                                                       ggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctt            >  2:2523337/1‑100 (MQ=255)
                                                                                        gcgTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgccttt           >  1:203325/1‑100 (MQ=255)
                                                                                         ctttAACGCCTTATCCGGGCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttg          <  2:2347513/98‑1 (MQ=255)
                                                                                          gTTAACGCCTTATCCGGCCTACAAAATCGTGCAAGTTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATAtttccctttgc         <  1:1359742/100‑1 (MQ=255)
                                                                                            tAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgtttttttta       >  1:373132/1‑91 (MQ=255)
                                                                                            tAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCa       <  2:1264669/100‑1 (MQ=255)
                                                                                             aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCAt      >  2:2478723/1‑100 (MQ=255)
                                                                                             aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCAt      >  2:2476843/1‑100 (MQ=255)
                                                                                             aaCGCCTTATCCGGCCTACAAAATCGTACAAATTCAATACATTGCAGAGTT‑‑‑TTC‑‑GTAGGGCTGATAAGCGGAGCGCATCAGGCATGtttgtctttgccct      >  1:1570042/1‑98 (MQ=18)
                                                                                                 cgttATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCTTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAg   <  2:2508572/97‑1 (MQ=255)
                                                                                                 ccTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGt  >  2:933861/1‑100 (MQ=255)
                                                                                                   |                                                                                                          
TTGCTGCCGGATGGCGCTGCACGCAGAGGCACAGCGCCACCGGGTATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAATACATTGCAGAATT‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGT  >  NC_000913/3705885‑3706081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: