Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,832,191 C→G 15.1% intergenic (+25/+28) yicH → / ← yicI putative inner membrane‑anchored periplasmic AsmA family protein/putative alpha‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,832,1910CG15.1% 358.9 / 49.6 132intergenic (+25/+28)yicH/yicIputative inner membrane‑anchored periplasmic AsmA family protein/putative alpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base C (55/56);  new base G (12/8);  total (68/64)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 135 total aligned reads displayed.

CAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAA  >  NC_000913/3832092‑3832288
                                                                                                   |                                                                                                 
cagcaAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTc                                                                                                   <  2:933878/100‑1 (MQ=255)
cagcaAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTc                                                                                                   >  1:399686/1‑100 (MQ=255)
   caAGTGACTCAAACAATGAATGCTGGCATTGTTTGCAGGGGTGAAGTTACAACGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAgn                                                                                                <  1:1555159/100‑2 (MQ=255)
    aaGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGAc                                                                                               >  1:368908/1‑100 (MQ=255)
     aGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACg                                                                                              <  2:2444664/100‑1 (MQ=255)
        gACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTAc                                                                                           <  2:587487/100‑1 (MQ=255)
        gACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTAc                                                                                           >  2:1087889/1‑100 (MQ=255)
           tCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCg                                                                                        <  2:1462225/100‑1 (MQ=255)
            cAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTACCCgg                                                                                       <  2:718611/100‑1 (MQ=39)
            cAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTACCCgg                                                                                       <  2:1083192/100‑1 (MQ=39)
            cAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCgg                                                                                       >  2:1033664/1‑100 (MQ=255)
            cAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCgg                                                                                       >  2:1153847/1‑100 (MQ=255)
             aaaCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTACCCGGc                                                                                      <  2:1271109/100‑1 (MQ=39)
             aaaCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTACCCGGc                                                                                      <  1:1803109/100‑1 (MQ=39)
             aaaCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTACCCGGc                                                                                      <  2:1345158/100‑1 (MQ=39)
             aaaCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGc                                                                                      >  1:1500633/1‑100 (MQ=255)
             aaaCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAACCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGc                                                                                      >  1:47469/1‑100 (MQ=255)
              aaCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGTCTGAGTCGTGCCCggag                                                                                     >  2:861815/1‑98 (MQ=255)
              aaCCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCa                                                                                     >  1:2566790/1‑100 (MQ=255)
                   tGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCa                                                                                <  1:1250791/100‑1 (MQ=255)
                     aaTGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATc                                                                              <  1:1052945/100‑1 (MQ=255)
                     aaTGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATc                                                                              <  1:1305420/100‑1 (MQ=255)
                       tGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCaa                                                                            <  2:1900469/100‑1 (MQ=255)
                        gCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAAt                                                                           >  2:1575307/1‑100 (MQ=255)
                        gCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAAt                                                                           >  2:1574540/1‑100 (MQ=255)
                         cTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATa                                                                          >  1:2344980/1‑100 (MQ=255)
                          tGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAg                                                                         <  2:1981419/100‑1 (MQ=255)
                          tGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAg                                                                         >  1:271007/1‑100 (MQ=255)
                           ggCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCa                                                                             >  1:2459527/1‑95 (MQ=255)
                            gCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGAt                                                                       >  2:246285/1‑100 (MQ=255)
                             aaTCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATATCGCCGGGTACGACTCAGACGTACCCGGAATTCCATCAATACAtt                                                                      >  1:1946530/2‑100 (MQ=255)
                                  tttCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAAc                                                                 <  1:845870/100‑1 (MQ=255)
                                  tttCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAAc                                                                 <  1:1109683/100‑1 (MQ=255)
                                      ccgCGGTGAAGTTCCATCGACGGAGCCGGTGCGGTAAACATCGATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTaa                                                             >  2:1750296/3‑100 (MQ=255)
                                       aGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAAt                                                            >  1:1424458/1‑100 (MQ=255)
                                       aGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAAt                                                            <  2:809363/100‑1 (MQ=255)
                                         cGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTg                                                          <  1:532492/100‑1 (MQ=255)
                                         cGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTg                                                          <  2:2201306/100‑1 (MQ=255)
                                          ggTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGt                                                         <  2:901055/100‑1 (MQ=255)
                                           gTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTc                                                        >  2:593190/1‑100 (MQ=255)
                                             gAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCan                                                      <  1:1345430/100‑2 (MQ=255)
                                             gAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAg                                                      <  2:2341808/100‑1 (MQ=255)
                                               aGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAgcg                                                    <  1:487716/100‑1 (MQ=255)
                                               aGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAgcg                                                    <  1:1553556/100‑1 (MQ=255)
                                                    cATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTc                                               <  1:2447657/100‑1 (MQ=255)
                                                    cATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTc                                               >  1:2098593/1‑100 (MQ=255)
                                                     aTCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATTGATTACAACGTAATTGTCAGCGCATTcc                                              <  1:70236/100‑1 (MQ=255)
                                                      tCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTccc                                             >  2:31899/1‑100 (MQ=255)
                                                       cGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCt                                            <  1:1473798/100‑1 (MQ=255)
                                                       cGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCt                                            <  1:644661/100‑1 (MQ=255)
                                                        gACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCtt                                           >  1:2433677/1‑100 (MQ=255)
                                                        gACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCtt                                           >  2:1587645/1‑100 (MQ=255)
                                                           ggAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAg                                        <  1:2200444/100‑1 (MQ=255)
                                                           ggAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAg                                        <  1:314429/100‑1 (MQ=255)
                                                            gAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAgg                                       >  1:805761/1‑100 (MQ=255)
                                                             aGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGc                                      <  1:628092/100‑1 (MQ=255)
                                                              gCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCt                                     <  1:1153843/100‑1 (MQ=255)
                                                               ccGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCtt                                    >  1:1406202/1‑100 (MQ=255)
                                                               ccGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCtt                                    >  2:261289/1‑100 (MQ=255)
                                                                cGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTc                                   >  1:1920693/1‑100 (MQ=255)
                                                                cGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTc                                   <  1:1028864/100‑1 (MQ=255)
                                                                 gttGCGGTAAACCTCAAGCTCGCCGTGGACGTCGCAGACGTACCCGGCATTCCCTCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTca                                  <  1:2339800/98‑1 (MQ=255)
                                                                 ggTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTca                                  <  1:951710/100‑1 (MQ=255)
                                                                 ggTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTca                                  >  2:1690265/1‑100 (MQ=255)
                                                                 ggTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTca                                  >  2:1518163/1‑100 (MQ=255)
                                                                      ggTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCaccacc                             <  2:2098607/100‑1 (MQ=255)
                                                                       gTAAACATCACTCTCGCCGGTTACGACTCAGACGTACCCGGCATTCCATCAATCTATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCAccacca                            <  2:2593820/100‑1 (MQ=255)
                                                                        tAAACATCAATCTCGCCGGGTACGACTAAGACGTACCCGGCATTCCATCCATCGATTACAACGTAATTGTCCGCGCATTCCCTTGAGGCttacccaac                             >  1:558457/1‑91 (MQ=255)
                                                                         aaaCATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGc                          >  2:170000/1‑100 (MQ=255)
                                                                          aaCATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGcc                         <  2:2470840/100‑1 (MQ=255)
                                                                           aCATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCAGCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGccc                        <  2:2162994/100‑1 (MQ=255)
                                                                            cATCAATCTCGCCGGGTACGGCTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGcccc                       <  1:1090468/100‑1 (MQ=255)
                                                                            cATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGcccc                       >  2:1690410/1‑100 (MQ=255)
                                                                            cATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGcccc                       <  1:748339/100‑1 (MQ=255)
                                                                              tCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTg                     >  1:972491/1‑100 (MQ=255)
                                                                              tCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTg                     >  1:897562/1‑100 (MQ=255)
                                                                                tggaatgCCGGGTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCt                   >  2:1015983/7‑100 (MQ=37)
                                                                                aaTCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCt                   >  1:1799655/1‑100 (MQ=255)
                                                                                  tctcGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCa                 <  1:194560/100‑1 (MQ=255)
                                                                                  tctcGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCa                 <  2:832670/100‑1 (MQ=255)
                                                                                    atgCCGGGTACGTCTGAGTCGTACCCGGCGTTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACt               >  1:901780/3‑100 (MQ=38)
                                                                                    atgCCGGGTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACt               >  1:1034186/3‑100 (MQ=38)
                                                                                     cGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACtt              <  1:2188855/100‑1 (MQ=255)
                                                                                      gCCGGGTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACttt             >  2:1315521/1‑100 (MQ=255)
                                                                                      gCCGGGTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACtt              >  1:1651191/1‑99 (MQ=255)
                                                                                       gcGGCTACGTCTGAGCCGTACCCGGCATTCCATCAATAGATTCCGCACTATTAGAGACCGCATTCCCTTGCGGCTTCACCACCAGCCCCTGc                    >  1:38754/2‑92 (MQ=255)
                                                                                       ccGGGTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTc            >  2:1152375/1‑100 (MQ=255)
                                                                                       ccGGGTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTc            >  2:2264898/1‑100 (MQ=39)
                                                                                       ccGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTc            >  2:2065063/1‑100 (MQ=255)
                                                                                       ccGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTc            >  1:577396/1‑100 (MQ=255)
                                                                                        cGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCa           >  1:1177155/1‑100 (MQ=255)
                                                                                        cGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCa           >  2:44434/1‑100 (MQ=255)
                                                                                         gggTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAg          >  2:1392333/1‑100 (MQ=255)
                                                                                         gggTACGTCTGAGTCGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAg          >  1:99123/1‑100 (MQ=255)
                                                                                         gggTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAg          <  2:47470/100‑1 (MQ=255)
                                                                                           gTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGc         <  1:238743/99‑1 (MQ=255)
                                                                                             aCGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTg      <  2:469160/100‑1 (MQ=255)
                                                                                              cGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGc     >  2:1790163/1‑100 (MQ=255)
                                                                                                 cTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGTGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGaa  >  2:2158512/1‑100 (MQ=255)
                                                                                                 cTCAGACGTACCCGGCATTCCAACAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGaa  >  1:1505125/1‑100 (MQ=255)
                                                                                                   |                                                                                                 
CAGCAAGTGACTCAAACCATGAATGCTGGCATCGTTTCCAGCGGTGAAGTTACATCGACGGAGCCGGTGCGGTAAACATCAATCTCGCCGGGTACGACTCAGACGTACCCGGCATTCCATCAATAGATTACAACGTAATTGTCAGCGCATTCCCTTGAGGCTTCACCACCAGCCCCTGCTCACTTTCAGCCTGCGAA  >  NC_000913/3832092‑3832288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: