| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 426,471 | G→A | 100% | R112H (CGT→CAT) | tgt → | tRNA‑guanine transglycosylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 426,471 | 0 | G | A | 96.4% | 261.0 / ‑0.0 | 83 | R112H (CGT→CAT) | tgt | tRNA‑guanine transglycosylase |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (39/41); total (40/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
ATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTTGATG > NC_000913/426374‑426566 | aTGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATa < 2:2048299/100‑1 (MQ=255) tGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATaa > 1:813949/1‑100 (MQ=255) tGCAGTGCAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACCGGGCGTGCACTTCCATa > 1:534198/1‑99 (MQ=255) aGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAAccc < 1:603590/100‑1 (MQ=255) gggCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACg > 2:460532/1‑100 (MQ=255) ggCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGGGCACTTCCATAACCCGATCAACgg > 2:1844314/1‑100 (MQ=255) gCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGc < 2:588851/100‑1 (MQ=255) gCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGc < 2:1461623/100‑1 (MQ=255) gCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGc < 1:2177270/100‑1 (MQ=255) gCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGc < 1:1832434/100‑1 (MQ=255) ccGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCg < 1:40630/100‑1 (MQ=255) ccGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCg > 2:1487717/1‑100 (MQ=255) ccGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCg > 2:2061418/1‑100 (MQ=255) cTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGa > 2:118040/1‑100 (MQ=255) ccGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCATCGGCGATCCGAtttt > 2:2559622/1‑100 (MQ=255) ccGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGAtttt < 2:1390890/100‑1 (MQ=255) gACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTcc < 1:907048/100‑1 (MQ=255) aCTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCt < 1:172566/100‑1 (MQ=255) cTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTc < 2:582356/100‑1 (MQ=255) cggcggCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGAt < 2:179113/100‑1 (MQ=255) ggcggcTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATc > 2:1116103/1‑100 (MQ=255) ggcggcTTCCAGGTCTTCAGCCTTGGCGATATTCGT‑AAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATcc > 1:2057723/1‑100 (MQ=255) gcggcTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATcc < 1:226213/100‑1 (MQ=255) ggcTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTg > 2:1359378/1‑100 (MQ=255) ggcTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTg > 1:152220/1‑100 (MQ=255) gcTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGa < 2:2409643/100‑1 (MQ=255) cTTCCAGGTTTTCAGCCTTTGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATcc < 1:2155458/96‑1 (MQ=255) ttCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGaaa < 1:39450/100‑1 (MQ=255) tCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGaaa > 2:195865/1‑99 (MQ=255) ccAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGaaaaa > 2:1727008/1‑100 (MQ=255) cAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAAt > 1:1054831/1‑100 (MQ=255) cAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAAt > 2:220600/1‑100 (MQ=255) aGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATc > 1:1502432/1‑100 (MQ=255) tCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAAt < 1:2404893/100‑1 (MQ=255) tCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAAt < 2:313892/100‑1 (MQ=255) cTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATg < 2:933636/100‑1 (MQ=255) cTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATg < 2:156416/100‑1 (MQ=255) tCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGga < 2:391807/100‑1 (MQ=255) tCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGga > 2:1899025/1‑100 (MQ=255) cAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGgag < 2:617593/100‑1 (MQ=255) aGCCTTGGCGATATTCGTAAAATCTCCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGgaga < 1:847184/100‑1 (MQ=255) gCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGAt > 1:1837598/1‑100 (MQ=255) cTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTc < 2:363436/100‑1 (MQ=255) ttGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCa < 1:490902/100‑1 (MQ=255) tGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAg > 2:498775/1‑100 (MQ=255) gCGATATTCGTAAAATCACCGAACAGGCCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTa < 1:874854/100‑1 (MQ=255) cGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTAc > 2:466961/1‑100 (MQ=255) atTCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATc < 1:1530922/100‑1 (MQ=255) tagTAAAATCACCCAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCtt < 2:1635192/98‑1 (MQ=255) tCGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCtt > 1:77366/1‑100 (MQ=255) cGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTg < 2:1502441/100‑1 (MQ=255) cGTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTg > 2:29897/1‑100 (MQ=255) gTAAAATCGCCGAGCGGGGCGTGTACTTCCATAGCCCGAGCAACGTCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACTATCTTgg < 1:483164/100‑1 (MQ=255) gTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTgg > 1:898277/1‑100 (MQ=255) gTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTgg > 2:54910/1‑100 (MQ=255) gTAAAATCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATGCAGTACGATCTTgg < 2:125151/100‑1 (MQ=255) aaaTCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGACCTTGGtt > 1:359389/1‑99 (MQ=255) aaTCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCg > 1:136214/1‑100 (MQ=255) agcACCCAACCTGGCGTGCACTTCCATAACCCGATCAACGTCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTTGTTCgg < 2:2188718/98‑1 (MQ=255) aTCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCgg < 1:1186220/100‑1 (MQ=255) aTCACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCgg < 2:952400/100‑1 (MQ=255) cACCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGa < 2:479862/99‑1 (MQ=255) aCCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCATTGGAGATTCAGTACGATCTTGGTTCGGata > 1:1207443/1‑100 (MQ=255) aCCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTCCGATCTTGGTTCGGata > 2:214804/1‑100 (MQ=255) aCCGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCAGata > 2:1866518/1‑100 (MQ=255) cGAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATc > 2:2329857/1‑100 (MQ=255) gAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCg > 2:926209/1‑100 (MQ=255) gAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCg > 1:165064/1‑100 (MQ=255) gAACAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCg > 1:425851/1‑100 (MQ=255) aaCAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGt > 2:2329763/1‑100 (MQ=255) aaCAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGt < 1:286200/100‑1 (MQ=255) aaCAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGt > 2:785949/1‑100 (MQ=255) aaCAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGt < 2:1136706/100‑1 (MQ=255) aCAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTc < 2:156526/100‑1 (MQ=255) cAGGGCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTACTCGATACTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTcn < 1:743760/100‑2 (MQ=255) ggCGTGCACTTCAATAACCCGATCAACGGAGATCCGATTTTCCTCGATACTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGa > 1:1170966/1‑100 (MQ=255) gCGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGAt < 2:814333/100‑1 (MQ=255) cGTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATc > 1:1736566/1‑100 (MQ=255) gTGCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCt < 2:1881468/100‑1 (MQ=255) gCACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGACCCTGAAAAATCAATGGAGATTCAGTCCGATCTTGGTTCGGATATCGTCATGATCttt < 2:874597/100‑1 (MQ=255) cACTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTtg > 2:1201188/1‑100 (MQ=255) cTTCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTtgat > 1:916236/1‑100 (MQ=255) ttCCATAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTtgatg < 1:1039774/100‑1 (MQ=255) | ATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATTTTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATGATCTTTGATG > NC_000913/426374‑426566 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |