Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,128,259	A→G	100%	V46A (GTG→GCG)	metJ ←	transcriptional repressor, S‑adenosylmethionine‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,128,259	0	A	G	100.0%	345.0 / NA	100	V46A (GTG→GCG)	metJ	transcriptional repressor, S‑adenosylmethionine‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (53/47);  total (53/47)

ACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCT  >  NC_000913/4128162‑4128355                                                                                                  |                                                                                                 aCGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGcc                                                                                                >  1:1180954/1‑100 (MQ=255) aCGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAACTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGcc                                                                                                >  1:1362979/1‑100 (MQ=255)  cGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCt                                                                                               >  2:1049270/1‑100 (MQ=255)  cGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCt                                                                                               <  2:2251938/100‑1 (MQ=255)   gCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTg                                                                                              <  1:1520176/100‑1 (MQ=255)    cAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTga                                                                                             <  2:1032090/100‑1 (MQ=255)    cAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTga                                                                                             >  2:2214662/1‑100 (MQ=255)      gATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTgacg                                                                                           >  1:2042828/1‑100 (MQ=255)       aTCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTgacga                                                                                          >  2:207222/1‑100 (MQ=255)         cGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGacgacg                                                                                        <  1:680087/100‑1 (MQ=255)          ggCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGAcgc                                                                                       >  1:2064744/1‑100 (MQ=255)          ggCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGAGGTTGGTAGCATGACGCAGGTTGTTCGCCTGACAAcgc                                                                                       >  1:2198905/1‑100 (MQ=255)           gCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGAcgcg                                                                                      >  2:1539210/1‑100 (MQ=255)           gCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGAcgcg                                                                                      >  1:1519607/1‑100 (MQ=255)           gCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGAcgcg                                                                                      <  2:2562410/100‑1 (MQ=255)             atcatcCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGTTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACgcgtg                                                                                    <  2:2472051/100‑1 (MQ=255)             atcatcCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACgcgtg                                                                                    <  2:2248666/100‑1 (MQ=255)             atcatcCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACCACgcgtg                                                                                    <  1:2155233/100‑1 (MQ=255)                  ccGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTc                                                                               >  2:699573/1‑100 (MQ=255)                     gCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATc                                                                            <  2:1307284/100‑1 (MQ=255)                        aaGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGt                                                                         >  2:1650645/1‑100 (MQ=255)                           gTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAg                                                                      <  2:381788/100‑1 (MQ=255)                           gTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAg                                                                      >  2:337081/1‑100 (MQ=255)                            ttGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAgg                                                                     >  2:2028347/1‑100 (MQ=255)                             tGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGa                                                                    >  2:1352835/1‑100 (MQ=255)                              gCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGAt                                                                   >  1:500288/1‑100 (MQ=255)                              gCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGAt                                                                   >  1:494305/1‑100 (MQ=255)                              gCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCATTCATCGGTGAGGAt                                                                   >  2:1531808/1‑100 (MQ=255)                               cccGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATTGGCGAGGAtt                                                                  >  2:739413/1‑100 (MQ=255)                                 cGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGAtttt                                                                >  2:2450126/1‑100 (MQ=255)                                 cGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGAtttt                                                                <  1:662477/100‑1 (MQ=255)                                  gttAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGAtttt                                                                <  1:778017/97‑1 (MQ=255)                                  ggTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGCCGCGTGCGTTCATCGGTGAGGAttttt                                                               <  1:1530118/100‑1 (MQ=255)                                  ggTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGAttttt                                                               <  1:469770/100‑1 (MQ=255)                                   gTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTa                                                              <  1:1963187/100‑1 (MQ=255)                                      aaagCATGCAGACACGCTTCGCACAGCAGCTCGCTGCTGGTAGCGAGACGCAGGGTGTTCGCCCGACGACGCGCGCGTTGATCGGTGAGGATTATTAac                                                            >  1:908891/4‑99 (MQ=255)                                      aaGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGAttttt                                                               >  1:756169/1‑96 (MQ=255)                                        ggCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAAcccc                                                         >  1:1665131/1‑100 (MQ=255)                                         gCATGCAGAAACGCTTCGCACAGCAGATCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCt                                                        >  1:2004819/1‑100 (MQ=255)                                          cATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCtt                                                       >  1:2551588/1‑100 (MQ=255)                                          cATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCtt                                                       <  1:2566554/100‑1 (MQ=255)                                          cATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCtt                                                       >  1:1734484/1‑100 (MQ=255)                                          cATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCtt                                                       >  2:2075763/1‑100 (MQ=255)                                           aTGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTa                                                      <  2:1317356/100‑1 (MQ=255)                                            tGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTaa                                                     >  2:1179834/1‑100 (MQ=255)                                            tGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTaa                                                     >  1:698121/1‑100 (MQ=255)                                             gCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAag                                                    >  2:1893253/1‑100 (MQ=255)                                              cAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAaga                                                   >  2:2521258/1‑100 (MQ=255)                                              cAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAaga                                                   <  2:2484839/100‑1 (MQ=255)                                                gAAACGCTTCGCACAGCAGCTCGCTGTTGGCAGCGTGACGCAGGTTGTTCGCCTGACGCCGCGTGCGTTCATCGGTGAGGATTTTTCACACCTTAAGAgg                                                 >  1:1220352/1‑100 (MQ=255)                                                  aaCGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGaa                                               >  1:231219/1‑100 (MQ=255)                                                     gCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGCCGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGACTgg                                            >  1:420569/1‑100 (MQ=255)                                                     gCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATgg                                            >  1:238934/1‑100 (MQ=255)                                                      cTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGa                                           <  1:2165723/100‑1 (MQ=255)                                                          gCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAAcc                                       >  1:1344212/1‑100 (MQ=255)                                                           cacaGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCg                                      <  2:276330/100‑1 (MQ=255)                                                            acaGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGt                                     <  2:841696/100‑1 (MQ=255)                                                               gcagcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAAt                                  >  2:2293073/1‑100 (MQ=255)                                                                  ncTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCtt                               >  1:1071331/2‑100 (MQ=255)                                                                  gcTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCtt                               >  2:970453/1‑100 (MQ=255)                                                                   cTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCttt                              <  2:102278/100‑1 (MQ=255)                                                                    tCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCtttt                             >  1:2403162/1‑100 (MQ=255)                                                                    tCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCtttt                             <  1:1352827/100‑1 (MQ=255)                                                                     cGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCttttt                            >  1:1677720/1‑100 (MQ=255)                                                                     cGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCttttt                            <  2:1519464/100‑1 (MQ=255)                                                                      ggtGTTGGTCGCGTGACGCAGGGCGTTGGCCTCACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTg                           <  2:2471185/98‑1 (MQ=255)                                                                      gCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTg                           <  2:992242/100‑1 (MQ=255)                                                                      gCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTg                           <  1:1299224/100‑1 (MQ=255)                                                                      gCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTg                           <  1:1299273/100‑1 (MQ=255)                                                                      gCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTg                           <  1:1650636/100‑1 (MQ=255)                                                                      gCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTg                           <  1:886047/100‑1 (MQ=255)                                                                      gCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTGAAGAGGAATGGAAACCGTAATCTTTTTg                           <  1:1805784/100‑1 (MQ=255)                                                                        tGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGAc                         <  1:2008342/100‑1 (MQ=255)                                                                         gTTTGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACt                        >  1:1175665/1‑100 (MQ=255)                                                                         gTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACt                        >  1:1083901/1‑100 (MQ=255)                                                                         gTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACt                        <  1:1954216/100‑1 (MQ=255)                                                                          ttGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACtt                       <  1:766441/100‑1 (MQ=255)                                                                           tGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTg                      >  1:1811083/1‑100 (MQ=255)                                                                            ggTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGt                     >  2:1419005/1‑100 (MQ=255)                                                                            ggTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGt                     >  1:890603/1‑100 (MQ=255)                                                                             gTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTGAGAGGAATGGAAACCGTAATCTTTTTGACTTGtt                    >  1:1360088/1‑100 (MQ=255)                                                                             gTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGtt                    >  2:962/1‑100 (MQ=255)                                                                             gTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGtt                    <  2:2559979/100‑1 (MQ=255)                                                                              tAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTc                   <  2:983727/100‑1 (MQ=255)                                                                              tAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTc                   <  2:983506/100‑1 (MQ=255)                                                                               aGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCa                  >  1:2303590/1‑100 (MQ=255)                                                                                gCGTGACGCGGGTTGTTCGCCTGACGTCGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCAc                 <  1:213165/100‑1 (MQ=255)                                                                                gCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAGCCGTAATCTTTTTGACTTGTTCAc                 <  1:667318/100‑1 (MQ=255)                                                                                   tGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCActct              <  2:454222/100‑1 (MQ=255)                                                                                    gACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACtctt             >  1:1239601/1‑100 (MQ=255)                                                                                     aCGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACtcttc            <  1:1539201/100‑1 (MQ=255)                                                                                      cGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACtcttct           <  1:2075749/100‑1 (MQ=255)                                                                                       gCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACtcttct           <  1:962/99‑1 (MQ=255)                                                                                       gCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTcttctt          >  2:1304498/1‑100 (MQ=255)                                                                                          ggTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGTTTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGc        >  2:1174156/1‑99 (MQ=255)                                                                                            ttgttCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTACCACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGt     <  2:2064757/100‑1 (MQ=255)                                                                                            ttgttCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGt     <  1:1708885/100‑1 (MQ=255)                                                                                            ttgttCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGt     <  2:1519616/100‑1 (MQ=255)                                                                                             tgttCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTg    <  2:1050933/100‑1 (MQ=255)                                                                                               ttCGCCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCt  >  1:1791157/1‑100 (MQ=255)                                                                                                  |                                                                                                 ACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCT  >  NC_000913/4128162‑4128355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑