Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,392,394 C→G 16.2% noncoding (35/76 nt) glyV → tRNA‑Gly

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,392,3940CG16.2% 266.1 / 44.1 107noncoding (35/76 nt)glyVtRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base C (43/45);  new base G (5/12);  total (48/59)
Fisher's exact test for biased strand distribution p-value = 1.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 113 total aligned reads displayed.

CGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAG  >  NC_000913/4392295‑4392493
                                                                                                   |                                                                                                   
cGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGc                                                                                                     >  2:2325270/1‑100 (MQ=255)
cGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGc                                                                                                     >  2:287162/1‑100 (MQ=255)
 gTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAACTTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGcc                                                                                                    <  2:338778/100‑1 (MQ=255)
 gTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGcc                                                                                                    >  1:105135/1‑100 (MQ=255)
    taATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAg                                                                                                 >  1:2489121/1‑100 (MQ=255)
     aaTGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAgg                                                                                                >  1:895194/1‑100 (MQ=255)
      aTGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGt                                                                                               >  2:620371/1‑100 (MQ=255)
         cgcCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCgg                                                                                            <  2:542554/100‑1 (MQ=255)
          gcCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCgtg                                                                                           <  2:43241/100‑3 (MQ=255)
          gcCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCgtg                                                                                           <  1:2343371/100‑3 (MQ=255)
          gcCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCgtg                                                                                           <  1:1991686/100‑3 (MQ=255)
          gcCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCgtg                                                                                           <  2:1100846/100‑3 (MQ=255)
          gcCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCgt                                                                                            <  1:2451445/99‑2 (MQ=255)
          gcCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCggg                                                                                           <  1:1712049/100‑1 (MQ=255)
          gcCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCggg                                                                                           <  2:2457343/100‑1 (MQ=255)
           ccTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCgtgc                                                                                          <  1:1734261/100‑4 (MQ=255)
           ccTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCgtgc                                                                                          <  2:342703/100‑4 (MQ=255)
           ccTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCgtgc                                                                                          <  2:828542/100‑4 (MQ=255)
            cTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCgtg                                                                                           <  1:2192560/98‑3 (MQ=255)
             tCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCATGGCAAGGTCgtgct                                                                                         <  1:273849/99‑5 (MQ=38)
              cccGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGACCCCGACCTTGGCAAGGTCgtgctct                                                                                       <  1:1532896/100‑7 (MQ=37)
                cGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcgcg                                                                                     <  2:1030988/100‑1 (MQ=255)
                cGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcgcg                                                                                     <  1:1023705/100‑1 (MQ=255)
                 gTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGa                                                                                    <  1:2167699/100‑1 (MQ=255)
                  tAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAg                                                                                   >  2:957377/1‑100 (MQ=255)
                  tAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAg                                                                                   >  2:1506339/1‑100 (MQ=255)
                  tAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAg                                                                                   >  2:956842/1‑100 (MQ=255)
                   aaCGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGt                                                                                  >  2:2186312/1‑100 (MQ=255)
                      gacgCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCg                                                                               <  1:2038570/100‑1 (MQ=255)
                        cgCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAg                                                                             >  1:1076228/1‑100 (MQ=255)
                         gaaGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAg                                                                             <  2:1332565/97‑1 (MQ=255)
                         gCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGt                                                                            <  1:2255178/100‑1 (MQ=255)
                          cAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtc                                                                           <  1:3611/100‑1 (MQ=255)
                          cAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGtc                                                                           >  2:1942785/1‑100 (MQ=255)
                                 gCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTc                                                                    <  2:165085/100‑1 (MQ=255)
                                 gCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTc                                                                    >  1:1072824/1‑100 (MQ=255)
                                  cGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCTAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTcc                                                                   <  1:1555766/100‑1 (MQ=255)
                                    aaaaTTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCg                                                                 >  1:1441415/1‑100 (MQ=255)
                                    aaaaTTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCg                                                                 >  2:2150283/1‑100 (MQ=255)
                                     aaaTTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGc                                                                <  2:520980/100‑1 (MQ=255)
                                     aaaTTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGc                                                                <  2:695294/100‑1 (MQ=255)
                                     aaaTTACGAAAGAAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGc                                                                <  1:1237019/100‑1 (MQ=255)
                                       aTTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTc                                                              <  2:252522/100‑1 (MQ=255)
                                         tACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGACGGGGTCGCGAGTTCGAGTCTCGTTTCCCGATCCa                                                            >  2:477301/1‑100 (MQ=255)
                                          aCGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCa                                                            >  2:2347440/1‑99 (MQ=255)
                                           cGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaaa                                                          >  2:583831/1‑100 (MQ=255)
                                           cGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaaa                                                          >  2:1891642/1‑100 (MQ=255)
                                           cGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaaa                                                          >  2:2587308/1‑100 (MQ=255)
                                             aaaGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAAt                                                        <  2:1003419/100‑1 (MQ=255)
                                              aaGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAAtt                                                       >  1:2390611/1‑100 (MQ=255)
                                              aaGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAAtt                                                       >  2:2377893/1‑100 (MQ=255)
                                               aGCAAAATTACCAAGCACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaa                                                           <  1:620368/95‑1 (MQ=11)
                                                gCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTg                                                     <  1:60570/100‑1 (MQ=255)
                                                gCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTg                                                     <  2:1704606/100‑1 (MQ=255)
                                                gCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTg                                                     >  2:852482/1‑100 (MQ=255)
                                                gCAAAATTAAGTAGGACGCGGGAATAGCGCAGTTGCTAGAGCACGACCTTGTCAAGTTTTGCCTAGCGTGTTCGCGTCTCGTTTCCCGCTCCAAAAttt                                                      <  1:400672/99‑1 (MQ=14)
                                                 cAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaaattcttc                                                    >  1:787867/1‑94 (MQ=255)
                                                  aaaaTTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaaattct                                                      >  1:1155971/1‑93 (MQ=255)
                                                  aaaaTTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCaaattct                                                      >  1:2101406/1‑93 (MQ=255)
                                                  aaaaTTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCCCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGaa                                                   <  2:1822451/100‑1 (MQ=255)
                                                        aaGTAGTACGCGGGAATAGCTCAGTTGGTAGAGGACGACCTGGCCAAGGTCCGGGTCTCGAGTTCGAGTCTCGTTTCCCGCTCCAAGATTTGAAAAGtg                                              <  1:2114963/99‑1 (MQ=14)
                                                           tagtaCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGtgctgc                                          <  2:2259038/100‑1 (MQ=255)
                                                             gtaCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCaa                                        >  2:736083/1‑100 (MQ=255)
                                                               aCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAg                                      <  1:312344/100‑1 (MQ=255)
                                                               aCGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAg                                      >  2:312346/1‑100 (MQ=255)
                                                                    ggAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGcaca                                  <  1:1478134/99‑1 (MQ=255)
                                                                    ggAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAg                                 <  1:1684251/100‑1 (MQ=255)
                                                                    ggAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAACTGCTGCAAAGc                                     >  2:1675599/1‑96 (MQ=255)
                                                                     gAATAGCTCAGTTGGTAGAGCACAACCTTGCCAAGGTCGGCATCAAAAGTTAGAGTCTCATTCCCCGCTACAAAATTTGAAAAGTGCTGCAAAGCACAg                                 <  1:860836/99‑1 (MQ=14)
                                                                          gCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTGGAGTCTCTTTTCCCGCTCCGAGATTTGAAATGGGCTTCAGAGCACAGcccacc                           >  1:2543535/1‑100 (MQ=14)
                                                                           cTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGTTGCAGAGCACAG‑CCAccc                          >  2:1715992/1‑99 (MQ=14)
                                                                           cTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCAccc                          >  2:1954440/1‑100 (MQ=255)
                                                                            tCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGATCGCGAGCTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCAccc                          <  1:1054711/99‑1 (MQ=255)
                                                                                ttGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCg                     >  2:2376486/1‑100 (MQ=255)
                                                                                ttGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCg                     <  2:1515395/100‑1 (MQ=255)
                                                                                  ggTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCggg                   <  2:2559301/100‑1 (MQ=255)
                                                                                  ggTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCggg                   <  2:1830959/100‑1 (MQ=255)
                                                                                  ggTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCggg                   <  1:1826405/100‑1 (MQ=255)
                                                                                   gTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTGTCGTGTCCCGCTCCGAGTTGTGAAAAAGGGTGGCAAGGACAGACTACCCAAGGGGGa                  >  1:1078993/1‑100 (MQ=2)
                                                                                   gTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCggg                   <  2:1024630/99‑1 (MQ=255)
                                                                                   gTAGAGCACGACCTTGCCAAGGGCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGa                  <  1:1116991/100‑1 (MQ=255)
                                                                                    tAGAGCACGACCTTGCCAAGGTCGGGATCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGaa                 >  2:1580721/1‑100 (MQ=255)
                                                                                     agagCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAAt                <  2:1788867/100‑1 (MQ=255)
                                                                                        gCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGTTGCAAAGCCCAAGCGACCCAAGCGGGAATTGc             >  2:2037944/1‑100 (MQ=14)
                                                                                        gCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCCCAACCCACCCAAGCGGGAAAAGc             >  1:688533/1‑100 (MQ=14)
                                                                                        gCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGc             <  1:2506500/100‑1 (MQ=255)
                                                                                        gCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGc             <  2:857176/100‑1 (MQ=255)
                                                                                        gCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGc             <  2:2013921/100‑1 (MQ=255)
                                                                                        ccccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGCGTCTCGTTTCCCGCTCCAAAATTTGAAAACAGCTGCAAAGCACCGACCACCCAAGCGGGAATAGc             >  2:957137/4‑100 (MQ=14)
                                                                                        ccccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCCCAGACCACCCAAGCGGGAATAGc             >  2:2395977/4‑100 (MQ=255)
                                                                                        ccccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGc             >  2:1613845/4‑100 (MQ=255)
                                                                                        ccccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGc             >  2:1696689/4‑100 (MQ=255)
                                                                                           cGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCCCAGACCACCCAAGCGGGAATAGCTCa          >  2:2193315/1‑100 (MQ=255)
                                                                                           cGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCa          >  2:552350/1‑100 (MQ=255)
                                                                                              ccTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGCAAAGTACTGCAAAGCCCAGACCACCCAATCGGGAATAGCTCAGtt       >  1:486076/1‑100 (MQ=14)
                                                                                              ccTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGtt       >  2:2150910/1‑100 (MQ=255)
                                                                                                ttGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTgg     <  2:1076232/100‑1 (MQ=255)
                                                                                                  gCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTa   <  1:1973317/100‑1 (MQ=255)
                                                                                                   ccAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTag  >  2:1496027/1‑100 (MQ=255)
                                                                                                   ccAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTag  >  2:352562/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
CGTATAATGCGCCTCCCGTAACGACGCAGAAATGCGAAAATTACGAAAGCAAAATTAAGTAGTACGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAG  >  NC_000913/4392295‑4392493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑