Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,424,468 A→C 18.4% intergenic (+82/+48) ulaF → / ← yjfY L‑ribulose 5‑phosphate 4‑epimerase/YhcN family protein, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,424,4680AC18.4% 272.5 / 63.2 125intergenic (+82/+48)ulaF/yjfYL‑ribulose 5‑phosphate 4‑epimerase/YhcN family protein, periplasmic
Reads supporting (aligned to +/- strand):  ref base A (55/47);  new base C (10/13);  total (65/60)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.88e-01

Only 100 of 126 total aligned reads displayed.

TATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTT  >  NC_000913/4424369‑4424565
                                                                                                   |                                                                                                 
tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGa                                                                                                   >  2:241706/1‑100 (MQ=255)
   taCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAgg                                                                                                >  2:418005/1‑100 (MQ=255)
   taCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAgg                                                                                                >  1:1322219/1‑100 (MQ=255)
    aCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCATATTTTTTCCCCCTGCGAAggg                                                                                               >  1:119457/1‑100 (MQ=255)
      gggCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAggggg                                                                                             >  2:1189153/1‑100 (MQ=255)
       ggtaGAAGTAGAACACGCGCTGCGGAAATTTACTTCCTCGGGAGATAACTGGTCTAATTCCGTAGCCGTTTTTTAAAAAAAAGCCCCCTTCGCAgggg                                                                                              <  1:820714/95‑1 (MQ=255)
       ggCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgc                                                                                            >  2:707929/1‑100 (MQ=255)
       ggCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgc                                                                                            >  1:2285288/1‑100 (MQ=255)
       ggCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGAAGCCGATATTAAAAAAAAAGCCCCCTGCGAAGGGGgc                                                                                            <  2:2340924/100‑1 (MQ=255)
        gCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgca                                                                                           <  2:211048/100‑1 (MQ=255)
         cAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgcaa                                                                                          >  1:1459391/1‑100 (MQ=255)
         cAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgc                                                                                            >  1:808276/1‑98 (MQ=255)
          aGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgcaaa                                                                                         <  2:462699/100‑1 (MQ=255)
           gcaGTAGAACACGCGCTGCGGAAATTTCCTTACTCGCAAGATACCTTCTCTAACTCCGCAAACGTCTTTCAAAAAAAAGCCCCCTGCGAAGGGGgcaaag                                                                                        <  1:344717/98‑1 (MQ=255)
           gAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgcaaag                                                                                        <  2:1164749/100‑1 (MQ=255)
           gAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgcaaag                                                                                        <  1:2348245/100‑1 (MQ=255)
              gTAGACCTCGCGCTGCGCAAATTTCCTTCCTCGGGCGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAACAATGCCCCCTGCGAAGGGGgcaaag                                                                                        <  1:2482222/97‑1 (MQ=255)
              gTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgcaaagcaa                                                                                     >  2:1522775/1‑100 (MQ=255)
              gTAGAACACGCGCGGCGGAAATTTCCTTCCTCGGGAGATAACTGCTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgcttttttt                                                                                     <  1:1585193/100‑8 (MQ=255)
               tAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCGGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgctttttttt                                                                                    <  1:287678/100‑9 (MQ=38)
               tAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgctttttttt                                                                                    <  1:2115511/100‑9 (MQ=38)
               tAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgctttttttt                                                                                    <  2:784568/100‑9 (MQ=38)
               tAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgctttttttt                                                                                    <  2:1387196/100‑9 (MQ=38)
               tAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgctttttttt                                                                                    <  1:1400732/100‑9 (MQ=38)
               tAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgctttttttt                                                                                    <  1:1108891/100‑9 (MQ=38)
                aGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgcttttttttg                                                                                   <  1:924178/100‑10 (MQ=38)
                aGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgctttttttt                                                                                    <  1:2228309/99‑9 (MQ=38)
                aGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAAc                                                                                   >  2:2031757/1‑100 (MQ=255)
                 gAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTTCGCAGGGGgcttttttttga                                                                                  <  2:547199/100‑11 (MQ=38)
                 gAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACt                                                                                  <  2:1300511/100‑1 (MQ=255)
                  aaCACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTa                                                                                 <  1:190991/100‑1 (MQ=255)
                    cacGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATg                                                                               <  2:2059903/100‑1 (MQ=255)
                       gcgcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCa                                                                            <  1:1096846/100‑1 (MQ=255)
                            gCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTTTATAAAAATCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGtt                                                                       >  1:1761283/1‑100 (MQ=255)
                            gCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGtt                                                                       >  1:692839/1‑100 (MQ=255)
                             cGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCGCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGttt                                                                      >  2:1815297/1‑100 (MQ=255)
                             cGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGttt                                                                      >  2:451182/1‑100 (MQ=255)
                              ggAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTc                                                                     >  2:440840/1‑100 (MQ=255)
                              ggAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTc                                                                     >  2:602163/1‑100 (MQ=255)
                                 aaTTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGCTTCGtt                                                                  >  1:1669419/1‑100 (MQ=255)
                                 aaTTTCCTTCCTCGGGAGATAACTGGCCTAAGTCGGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGtt                                                                  <  1:1299640/100‑1 (MQ=255)
                                  aTTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTg                                                                 <  2:742943/100‑1 (MQ=255)
                                   tttCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTgg                                                                <  2:2285303/100‑1 (MQ=255)
                                   tttCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTgg                                                                <  2:1664780/100‑1 (MQ=255)
                                   tttCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTgg                                                                <  2:947231/100‑1 (MQ=255)
                                    ttccttccTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGt                                                               <  2:1346402/100‑1 (MQ=255)
                                      ccttcctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTta                                                             >  2:653301/1‑100 (MQ=255)
                                       cttcctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGtaga                                                            >  2:1326596/1‑97 (MQ=255)
                                        ttcctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtata                                                           <  2:1570177/100‑1 (MQ=255)
                                          cctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATAcc                                                         <  1:2362007/100‑1 (MQ=255)
                                           ctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtat                                                            <  1:521961/96‑1 (MQ=255)
                                           ctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCt                                                        >  2:1359220/1‑100 (MQ=255)
                                            tCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTg                                                       >  1:1447907/1‑100 (MQ=255)
                                              gggAGTTAATTGGTTTAATTCCGAAGCGTTTTTTAAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTgg                                                      <  2:2429424/99‑1 (MQ=255)
                                              gggAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGt                                                     <  1:442186/100‑1 (MQ=255)
                                                gagaTAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGc                                                   <  2:2356884/100‑1 (MQ=255)
                                                 agaTAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCt                                                  >  2:1457913/1‑100 (MQ=255)
                                                 agaTAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCt                                                  <  2:2559896/100‑1 (MQ=255)
                                                    tAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGc                                               <  1:333899/100‑1 (MQ=255)
                                                    tAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGc                                               >  1:958513/1‑100 (MQ=255)
                                                      aCTGTTCGAATGCCGCAGCCGTATTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAgt                                               <  1:183060/98‑2 (MQ=255)
                                                      aCTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGa                                             <  1:734359/100‑1 (MQ=255)
                                                       cTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGAt                                            >  2:194038/1‑100 (MQ=255)
                                                       cTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGAt                                            >  2:2026318/1‑100 (MQ=255)
                                                        tGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATa                                           <  2:1496061/100‑1 (MQ=255)
                                                         ggTCTAATTCCGCAGCCGTTTTTCAAAACAAAGCCCCCTGCGAAGGGGACAAAGCAAACTATGGCAATGTTCCGTTGGTTATACCTGGTGCTAGCGATa                                           >  1:621100/1‑99 (MQ=255)
                                                           tCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAat                                        >  2:749949/1‑100 (MQ=255)
                                                           tCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAat                                        >  1:219041/1‑100 (MQ=255)
                                                           tCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAat                                        >  2:2412825/1‑100 (MQ=255)
                                                            cTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAata                                       >  2:2440661/1‑100 (MQ=255)
                                                            cTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAAATATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAata                                       >  1:1655792/1‑100 (MQ=255)
                                                                ttCCGCAGCCGTTTTTTTTTAAAAATCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTCCTAGCGATAAATATCcg                                   >  1:175764/1‑100 (MQ=255)
                                                                    gaaGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCt                               >  2:1758924/3‑100 (MQ=255)
                                                                          gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTg                         <  1:1966841/100‑1 (MQ=255)
                                                                           tttttCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGc                        <  1:2590824/100‑1 (MQ=255)
                                                                            ttttCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCa                       >  2:1515468/1‑100 (MQ=255)
                                                                             tttCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCat                      >  2:1657441/1‑100 (MQ=255)
                                                                             tttCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCat                      <  2:283049/100‑1 (MQ=255)
                                                                               aaaaaaaaaaGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGtn                         <  1:2138644/93‑2 (MQ=255)
                                                                                cAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATAtt                   >  1:1464307/1‑100 (MQ=255)
                                                                                cAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATAtt                   >  2:1328337/1‑100 (MQ=255)
                                                                                 aaaaaaaaGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTg                  >  2:943298/1‑100 (MQ=255)
                                                                                  aaaaaaaGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGc                 >  1:1770383/1‑100 (MQ=255)
                                                                                   tgctttgCCCCCTTCGCAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGcc                >  2:1629906/7‑100 (MQ=255)
                                                                                   tgctttgCCCCCTTCGCAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAATATCCGCGCTGGCGTGCATATTGCCg               >  1:2083279/7‑100 (MQ=255)
                                                                                   aaaaaaGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGcc                <  1:1020454/100‑1 (MQ=255)
                                                                                    gctttgCCCCCTTCGCAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAACGATAAATATCCGCGCTGGCGTGCATATTGCCg               >  2:267237/6‑100 (MQ=255)
                                                                                     ctttgCCCCCTTCGCAGGGGGCAAAGCAAACTATGGCTCTGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGt              >  1:759922/5‑100 (MQ=255)
                                                                                     ctttgCCCCCTTCGCAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGt              >  2:968747/5‑100 (MQ=255)
                                                                                     ctttgCCCCCTTCGCAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGt              >  2:651572/5‑100 (MQ=255)
                                                                                        aGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTAc           >  2:1862854/1‑100 (MQ=255)
                                                                                        aGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTAc           <  2:1899463/100‑1 (MQ=255)
                                                                                         gCCCCCTTCGCAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACt          >  1:936993/1‑100 (MQ=255)
                                                                                            cccTTCGCAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTccc       >  2:1046353/1‑100 (MQ=255)
                                                                                            cccTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATGCCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTc         <  1:1568601/98‑1 (MQ=255)
                                                                                            cccTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTctc       >  1:1223497/1‑98 (MQ=255)
                                                                                             ccTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTcccc      <  1:1189149/100‑1 (MQ=255)
                                                                                             ccTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTcccc      >  2:308486/1‑100 (MQ=255)
                                                                                              cTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTaagatcgg     >  2:1189367/1‑93 (MQ=255)
                                                                                                 cGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGCGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGtt  <  2:1383352/100‑1 (MQ=255)
                                                                                                   |                                                                                                 
TATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTT  >  NC_000913/4424369‑4424565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: