| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 467,839 | T→C | 74.8% | V143A (GTC→GCC) | cof → | thiamine pyrimidine pyrophosphate hydrolase; HMP‑PP phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 467,839 | 0 | T | C | 74.8% | 113.6 / 33.3 | 67 | V143A (GTC→GCC) | cof | thiamine pyrimidine pyrophosphate hydrolase; HMP‑PP phosphatase |
| Reads supporting (aligned to +/- strand): ref base T (11/6); new base C (24/26); total (35/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
TTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTT > NC_000913/467743‑467933 | ttACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGCCAc < 2:1424784/100‑1 (MQ=255) ccGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGCCACCaa > 2:1558594/1‑100 (MQ=255) ccGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGCCACCaa > 2:510214/1‑100 (MQ=255) ggAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGCCACCAAGAt > 1:1824788/1‑100 (MQ=255) ggAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGCCACCAAGAt > 1:1824802/1‑100 (MQ=255) gAAAGCGAGCCCTGCGTTGTTGCAGGCTTTTGTCTATAGCGGGTTTCGTTACCAGATACTCGATGTCCAAAATATGCCACTGGGCAGCGCCACCAAGATc < 1:2268025/100‑1 (MQ=255) gAAAGAGATCCCTGCGTTGTTGTAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGCCACCAAGATc < 2:2417032/100‑1 (MQ=255) aaGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAGTGCCACTACGCAGCGCCACCCAGATCTg > 1:1781590/1‑100 (MQ=255) gagaTCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGCCACCAAGATCTGCt < 2:1553344/100‑1 (MQ=255) gaTCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTTAAAAAAATGCCTCTCGGCAGCGCCACCGAGATCTGCTTc < 1:1482222/100‑1 (MQ=255) aTCCCTGCGTTTTGGCAGGCATTCGTTTTTAGCTTTTTTCGTTATCAGATAATGGATGTTTAGAATATGCCTCGCGGCAGCGCCACCAAGATCTGCTTCt < 1:1390728/100‑1 (MQ=255) cccTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCtg > 1:362868/1‑99 (MQ=255) ccTGCGTTGTTGCAGGCATATGTCTATCGCGGTTTTCGTTATCAGATAATCAATGTAAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCtgtg < 1:2225942/100‑1 (MQ=255) tGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGc < 1:1000026/100‑1 (MQ=255) gCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCg > 1:1352615/1‑100 (MQ=255) tgttgCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCa < 2:307765/100‑1 (MQ=255) tgttgCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCa < 1:254512/100‑1 (MQ=255) ttgCAGGCATTTGGCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCTCTCGGCAGCTCCACCAAGATCTGCTTCTGTGGCGATCacg < 1:1181221/100‑1 (MQ=255) gCAGGCGTTTGTATATAGCGGTTTTCGTTATCAGATAATCGATGTCAAACACCTGCCACTCGGCAACGCCACTGAGATCTCCtt > 1:2508845/1‑84 (MQ=255) gCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCacgac < 2:2088542/100‑1 (MQ=255) gCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCacgac < 2:680219/100‑1 (MQ=255) ggCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCCCGACGAt > 1:2508070/1‑100 (MQ=255) cATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGCTGTCAAAAAAATGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCacgcc > 1:2195233/1‑93 (MQ=255) tttGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTa > 2:320158/1‑100 (MQ=255) tataGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCt > 1:1541776/1‑100 (MQ=255) taGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCAGCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTg > 1:1653666/1‑100 (MQ=255) aGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGc > 2:1072614/1‑100 (MQ=255) gCGGTTTTCGTTATCAGATAATCGCTGTCAAAAAAATGCCGCTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCa < 2:2415281/100‑1 (MQ=255) gCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCa < 1:2565363/100‑1 (MQ=255) cGGTTTTCGTTATCATATAATCGATGTCAAAAAAATGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAg < 2:2557502/100‑1 (MQ=255) cGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAg < 2:1286154/100‑1 (MQ=255) ggTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGa > 1:1037798/1‑100 (MQ=255) gTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGAt > 1:2459942/1‑100 (MQ=255) gTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGAt < 2:1541785/100‑1 (MQ=255) tttCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATcc < 2:1037801/100‑1 (MQ=255) cGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGc < 2:587667/100‑1 (MQ=255) cGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGc > 2:512997/1‑100 (MQ=255) cGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGCCGATCACGACGATCTTACACGCTTGCAGATCCAGc > 1:421556/1‑100 (MQ=255) gTTCTCAGATAAAAGAAATAAAAAAAATGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAg < 1:2130401/98‑1 (MQ=255) tATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCtat > 2:1453248/1‑100 (MQ=255) tATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCtat < 1:809603/100‑1 (MQ=255) tCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATAc > 2:1332622/1‑100 (MQ=255) aaaTAATCGATGTCAAAAAAATGCCACTCGGCAGCGCCACCATGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGa > 2:1750219/3‑100 (MQ=255) aGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACg > 2:1840948/1‑99 (MQ=255) aTAATCGATGTCAAAAAAATGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAg < 2:372954/100‑1 (MQ=255) tGTCAAAAAAATGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGc > 2:17352/1‑100 (MQ=255) gTCAAAAAAATGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCg > 2:1909029/1‑100 (MQ=255) cAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCg > 1:2175085/1‑98 (MQ=255) cAAAAAAATGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCTTTAGGc > 1:709712/1‑97 (MQ=255) cAAAAAAATGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAg > 2:389838/1‑100 (MQ=255) cAAAAAAATGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCAATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAg > 1:2354179/1‑95 (MQ=255) aaaaaaaTGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTCGACGCTTGCAGTTTCAGCTATCCGAAGCATTAGTCg > 1:1254151/1‑97 (MQ=255) aaaaaaTGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCg < 2:1722738/100‑1 (MQ=255) aaaaaaTGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCg > 2:2243708/1‑100 (MQ=255) aaaaTGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTg < 1:470234/100‑1 (MQ=255) aaaTGTCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGc < 2:582696/100‑1 (MQ=255) aaTGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGca < 1:2261025/100‑1 (MQ=255) tGCCACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGcaca < 1:2199798/100‑1 (MQ=255) ccACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGTACAt > 1:2216179/1‑99 (MQ=255) ccACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACAtt < 1:2541430/100‑1 (MQ=255) ccACTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACAtt > 2:361088/1‑100 (MQ=255) ccACTCGGCAGCGCCACCAAGATCTGCTTCTGTGACGATCACGACGAACTAACACGCTTGCAGCTCCAGCTCTGCGATGCATTAGCCGAGCGCGCACAt > 1:543122/1‑99 (MQ=255) aCTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTtg < 2:1195898/100‑1 (MQ=255) aCTCGGCAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTtg > 1:1970306/1‑100 (MQ=255) cAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGtttt < 2:347196/100‑1 (MQ=255) cAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATGCAGCTATACGAAGGATTATGCGAGCGGGCACATGTGTGtttt > 1:2524768/1‑100 (MQ=255) cAGCGCCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGtttt < 2:1978842/100‑1 (MQ=255) | TTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTT > NC_000913/467743‑467933 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |