Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 657,535 C→A 15.7% intergenic (+34/+20) cspE → / ← flc constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913657,5350CA15.7% 281.2 / 37.6 101intergenic (+34/+20)cspE/flcconstitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein
Reads supporting (aligned to +/- strand):  ref base C (45/40);  new base A (8/8);  total (53/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.97e-01

Only 100 of 101 total aligned reads displayed.

GCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACG  >  NC_000913/657437‑657633
                                                                                                  |                                                                                                  
gcgTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCa                                                                                                   >  2:1983557/1‑100 (MQ=255)
 cgTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAg                                                                                                  >  2:2533950/1‑100 (MQ=255)
   tAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCg                                                                                                >  2:79240/1‑100 (MQ=255)
        ttCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttt                                                                                           <  2:1471358/100‑1 (MQ=255)
         tCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttt                                                                                          >  2:1171315/1‑100 (MQ=255)
         tCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGtttt                                                                                          <  2:178261/100‑1 (MQ=255)
         tCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGtttt                                                                                          <  2:557298/100‑1 (MQ=255)
         tCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGtttt                                                                                          <  1:2580277/100‑1 (MQ=255)
          cGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttg                                                                                         <  2:732068/100‑2 (MQ=39)
           gAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttttt                                                                                        >  1:2100435/1‑100 (MQ=255)
           gAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttgn                                                                                        <  2:1872426/100‑3 (MQ=39)
            aaaTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGttttttt                                                                                       <  2:190423/100‑1 (MQ=255)
            aaaTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCGCTGTAAGATACGTCAGCAAGAATTCCAAACCCGCTTAATCAGCGGGtcttttt                                                                                       >  1:2481096/1‑100 (MQ=255)
             aaTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAGTTCAAAACCCGCTGATTAAGCGGGttttgaat                                                                                      <  2:2462295/100‑5 (MQ=38)
               tCACTAACGGTGCCAAAGGCCCTTCTACTGCAAACGCAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttgaa                                                                                       <  1:1669643/97‑4 (MQ=37)
                 aatAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGttttttt                                                                                       <  1:2477970/93‑1 (MQ=255)
                 aCTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTc                                                                                  >  2:1842786/1‑100 (MQ=255)
                  cTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGt                                                                                   >  1:823773/1‑98 (MQ=255)
                    aaCGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCttt                                                                               >  2:1014836/1‑100 (MQ=255)
                    aaCGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCttt                                                                               >  1:1731062/1‑100 (MQ=255)
                         tGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAgtgt                                                                          <  2:1345976/100‑1 (MQ=255)
                           ccAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGc                                                                        <  1:487159/100‑1 (MQ=255)
                           ccAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGc                                                                        <  2:1412281/100‑1 (MQ=255)
                           ccAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGc                                                                        >  1:361379/1‑100 (MQ=255)
                            cAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCg                                                                       >  1:2138534/1‑100 (MQ=255)
                            cAAAGGCCCTTCTGCTGCAAACGTAATCCCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCg                                                                       <  2:1289736/100‑1 (MQ=255)
                              aaGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGt                                                                     <  2:1993443/100‑1 (MQ=255)
                               aGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGtt                                                                    <  2:862971/100‑1 (MQ=255)
                                ggCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTg                                                                   <  2:2502507/100‑1 (MQ=255)
                                ggCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTg                                                                   >  1:1443292/1‑100 (MQ=255)
                                  cccTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAACCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAg                                                                 >  2:2362526/1‑100 (MQ=255)
                                  cccTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTATTCAGCGGGTTGTTTTTGGTCTTTAGTGTGCGGTTGAg                                                                 >  2:1597484/1‑100 (MQ=255)
                                  cccTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAg                                                                 <  2:497124/100‑1 (MQ=255)
                                    cTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGc                                                               <  2:87912/100‑1 (MQ=255)
                                          ctgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGaaaa                                                         >  2:2311561/1‑100 (MQ=255)
                                          ctgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAAGCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGaaaa                                                         <  2:856187/100‑1 (MQ=255)
                                           tgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAc                                                        <  2:1031763/100‑1 (MQ=255)
                                            gcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACa                                                       >  1:2304043/1‑100 (MQ=255)
                                             cAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAg                                                      >  2:1563146/1‑100 (MQ=255)
                                              aaaCGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGc                                                     <  1:1615497/100‑1 (MQ=255)
                                               aaCGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGcc                                                    >  1:710551/1‑100 (MQ=255)
                                               aaCGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGcc                                                    >  2:34292/1‑100 (MQ=255)
                                               aaCGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGcc                                                    <  2:588496/100‑1 (MQ=255)
                                               aaCGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGcc                                                    <  1:2035234/100‑1 (MQ=255)
                                               aaCGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGcc                                                    <  2:525172/100‑1 (MQ=255)
                                                aCGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCa                                                   <  2:2142937/100‑1 (MQ=255)
                                                  gTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGTCAGa                                                 <  1:1235066/100‑1 (MQ=255)
                                                     aTCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATg                                              >  1:728025/1‑100 (MQ=255)
                                                        gCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCa                                           <  1:1879593/100‑1 (MQ=255)
                                                         ctctGTAAGATACGTCAGCAAGAATTCAAATTCTGCTTAATCAGCGGGTTTGTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAg                                          <  2:2371370/100‑1 (MQ=255)
                                                         ctctGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAg                                          <  1:1767921/100‑1 (MQ=255)
                                                          tctGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTg                                        <  2:719784/100‑1 (MQ=255)
                                                            tGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGTTATTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTg                                        >  1:1588462/1‑99 (MQ=255)
                                                            tGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGc                                       <  1:1068571/100‑1 (MQ=255)
                                                               aaGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGt                                    <  2:2482271/100‑1 (MQ=255)
                                                                   tACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATg                                >  2:271270/1‑100 (MQ=255)
                                                                   tACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATg                                >  2:1922181/1‑100 (MQ=255)
                                                                   tACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGCTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATg                                <  1:1405361/100‑1 (MQ=255)
                                                                   naCGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATg                                >  1:1304697/2‑100 (MQ=255)
                                                                    aCGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATgg                               <  1:531171/100‑1 (MQ=255)
                                                                     cGTCAGCAAGGATGCAAGAGGCGCTGAAGCTGCGGGTGTTTGTTGGGCTTTAGTGGGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCAggg                               <  1:2086295/99‑1 (MQ=255)
                                                                     cGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGc                              <  2:1283011/100‑1 (MQ=255)
                                                                      gTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCaa                            >  1:488162/1‑100 (MQ=255)
                                                                        cAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCaaa                           >  2:112005/1‑100 (MQ=255)
                                                                        cAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCaaa                           >  2:1082285/1‑100 (MQ=255)
                                                                        cAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCaaa                           <  2:1647032/100‑1 (MQ=255)
                                                                          gCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTTGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAg                         <  1:657890/100‑1 (MQ=255)
                                                                          gCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAg                         >  2:468479/1‑100 (MQ=255)
                                                                          gCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAg                         >  2:2020252/1‑100 (MQ=255)
                                                                           cAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGa                        >  2:166401/1‑100 (MQ=255)
                                                                           cAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGa                        >  1:889834/1‑100 (MQ=255)
                                                                             aGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcc                      >  2:2385844/1‑100 (MQ=255)
                                                                             aGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcc                      >  1:661505/1‑100 (MQ=255)
                                                                              gAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAccc                     >  2:1634258/1‑100 (MQ=255)
                                                                              gAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAccc                     <  1:354985/100‑1 (MQ=255)
                                                                               caaaaaaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCTAGTGGGGTGATGGCAAAAGAccac                    >  1:1491506/6‑98 (MQ=25)
                                                                               caaaaaaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGACGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcccc                    >  1:125870/6‑100 (MQ=37)
                                                                               aaTTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcccc                    <  2:533244/100‑1 (MQ=255)
                                                                               aaTTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcccc                    <  2:1337289/100‑1 (MQ=255)
                                                                               aaTTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcccc                    <  2:2347885/100‑1 (MQ=255)
                                                                                aTTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAATACCCCa                   >  1:2559116/1‑100 (MQ=255)
                                                                                aTTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCa                   <  1:1090524/100‑1 (MQ=255)
                                                                                aTTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCa                   <  1:1171311/100‑1 (MQ=255)
                                                                                 ttCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAg                  >  2:358475/1‑100 (MQ=255)
                                                                                 aaaaaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAg                  >  2:565988/4‑100 (MQ=38)
                                                                                   cAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGaa                >  1:1370899/1‑100 (MQ=255)
                                                                                   cAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGaa                <  2:1058357/100‑1 (MQ=255)
                                                                                   cAAAACCCGCTTAATCAGCGGGTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAg               >  2:121714/1‑100 (MQ=255)
                                                                                    aaaaCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAg               >  2:884516/1‑100 (MQ=255)
                                                                                    aaaaCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAg               >  2:1787890/1‑100 (MQ=255)
                                                                                    aaaaCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAg               >  2:328306/1‑100 (MQ=255)
                                                                                    aaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAg               >  2:230597/1‑100 (MQ=255)
                                                                                     aaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAgg              >  1:370310/1‑100 (MQ=255)
                                                                                     aaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAgg              >  2:205921/1‑100 (MQ=255)
                                                                                     aaaCCCGCTGATTAAGCGGGTTTCTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAgg              >  1:1050554/1‑100 (MQ=255)
                                                                                      aaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGt             >  2:720580/1‑100 (MQ=255)
                                                                                         ccGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGAGGTAGAGGCAGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCAAGAAGGTTGa          >  1:1875831/1‑100 (MQ=255)
                                                                                            cTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGa       <  2:113386/100‑1 (MQ=255)
                                                                                                aTCAGCGGGTTTTTTTTTTTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAAc   >  1:134613/1‑100 (MQ=255)
                                                                                                 tCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACg  >  2:2073926/1‑100 (MQ=255)
                                                                                                  |                                                                                                  
GCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACG  >  NC_000913/657437‑657633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: