Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	715,849	A→G	100%	L146L (TTA→TTG)	ybfP →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	715,849	0	A	G	96.7%	283.1 / ‑1.0	89	L146L (TTA→TTG)	ybfP	lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/3);  new base G (49/37);  total (49/40)
Fisher's exact test for biased strand distribution p-value = 8.70e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.31e-01

CAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATG  >  NC_000913/715754‑715944                                                                                                |                                                                                                cAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTgcgac                                                                                             <  1:623089/100‑1 (MQ=255) cAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTgcgac                                                                                             >  2:847902/1‑100 (MQ=255) cAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTgcgac                                                                                             <  2:503356/100‑1 (MQ=255)  aGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTgcga                                                                                              <  1:1638918/98‑1 (MQ=255)     gCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTg                                                                                         >  1:1808930/1‑100 (MQ=255)       aaGCTCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGcc                                                                                       <  1:332700/100‑1 (MQ=255)       aaGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGcc                                                                                       <  1:251675/100‑1 (MQ=255)       aaGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGcc                                                                                       <  2:469035/100‑1 (MQ=255)         gCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAg                                                                                     >  2:2105801/1‑100 (MQ=255)         gCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAg                                                                                     <  1:2293495/100‑1 (MQ=255)           ccGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAg                                                                                     <  1:504016/98‑1 (MQ=255)           ccGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTc                                                                                   <  2:1252796/100‑1 (MQ=255)             ggCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAg                                                                                 <  1:608896/100‑1 (MQ=255)               ttccgatctgATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGaa                                                                               <  1:270507/91‑1 (MQ=255)                aCTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGTAATTATTGAGTTGCGACGCTCCCAGACAGaa                                                                               >  1:2529779/1‑99 (MQ=255)                   cGACCGATAGTGAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAAtt                                                                           >  1:1356324/1‑100 (MQ=255)                   cGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAAtt                                                                           >  1:396604/1‑100 (MQ=255)                    gACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTg                                                                          <  2:2170677/100‑1 (MQ=255)                     aCCTATAGTCAATGAGGATTATGTCAGCGAAAGCCGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTg                                                                          <  1:2441788/99‑1 (MQ=255)                      ccGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGcc                                                                        <  1:847899/100‑1 (MQ=255)                      ccGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGcc                                                                        <  1:1878194/100‑1 (MQ=255)                       cGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCa                                                                       <  2:281144/100‑1 (MQ=255)                        gATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGaaagatcggaa                                                                      >  2:270510/1‑92 (MQ=255)                        gATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAg                                                                      >  2:124797/1‑100 (MQ=255)                            gTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTTTTTTTTTTTTGTATGTCGGGATGATTGAGTTGCGACGCTTTCAGTCAGAAAATTGCCAGCAAg                                                                  >  1:99541/1‑100 (MQ=255)                            gTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAg                                                                  <  2:1478199/100‑1 (MQ=255)                              cAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGaa                                                                >  2:988605/1‑100 (MQ=255)                              cAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGaa                                                                >  1:714740/1‑100 (MQ=255)                                 tGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTg                                                             >  1:5057/1‑100 (MQ=255)                                  gAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGa                                                            >  2:1796435/1‑100 (MQ=255)                                   aGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGaa                                                           <  1:350759/100‑1 (MQ=255)                                    ggATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGaaa                                                          >  2:262859/1‑100 (MQ=255)                                    ggATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGaaa                                                          >  2:1295576/1‑100 (MQ=255)                                      aTTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGc                                                        <  2:426231/100‑1 (MQ=255)                                       ttATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCg                                                       >  1:1048654/1‑100 (MQ=255)                                       ttATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCg                                                       >  1:1470915/1‑100 (MQ=255)                                       ttATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCg                                                       <  1:1833451/100‑1 (MQ=255)                                         aTGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGa                                                      >  1:1306359/1‑99 (MQ=255)                                         aTGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGAt                                                     >  2:2263153/1‑100 (MQ=255)                                          tGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATa                                                    >  1:2183797/1‑100 (MQ=255)                                           gTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATaa                                                   >  1:2488985/1‑100 (MQ=255)                                           gTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATaa                                                   >  1:2327576/1‑100 (MQ=255)                                           gTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATGATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATaa                                                   >  1:2246830/1‑100 (MQ=255)                                             cAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAAt                                                 <  2:634926/100‑1 (MQ=255)                                             cAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAAt                                                 >  2:1918775/1‑100 (MQ=255)                                              aGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATa                                                >  2:268530/1‑100 (MQ=255)                                               gCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATac                                               >  2:527360/1‑100 (MQ=255)                                               gCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCGGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATac                                               >  1:1654108/1‑100 (MQ=255)                                                cGAAAGCGGATTTTTTGTCTTTTTGATGCATGTCGGGATTATTGAGTTGCGACGCTTCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATaca                                              >  1:1256105/1‑100 (MQ=255)                                                cGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATaca                                              <  1:1945225/100‑1 (MQ=255)                                                 gAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATacac                                             >  1:1990757/1‑100 (MQ=255)                                                     ncGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTa                                         >  2:719757/2‑100 (MQ=255)                                                     gCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTa                                         <  2:885981/100‑1 (MQ=255)                                                      cGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTaa                                        >  1:2543541/1‑100 (MQ=255)                                                      cGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTaa                                        >  2:882291/1‑100 (MQ=255)                                                           tttttGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAg                                   <  2:1607931/100‑1 (MQ=255)                                                             tttGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTg                                 <  1:1918623/100‑1 (MQ=255)                                                             tttGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTg                                 <  1:1467886/100‑1 (MQ=255)                                                             tttGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTg                                 <  2:786039/100‑1 (MQ=255)                                                             tttGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGACAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTg                                 <  1:391158/100‑1 (MQ=255)                                                                ggCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGt                              >  2:1819905/1‑100 (MQ=255)                                                                  cTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTcc                            >  2:109263/1‑100 (MQ=255)                                                                  cTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTcc                            >  2:1016849/1‑100 (MQ=255)                                                                    cGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCgg                          >  2:967540/1‑100 (MQ=255)                                                                    cGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCgg                          >  2:668987/1‑100 (MQ=255)                                                                    cGATGATACATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCgg                          >  2:1358261/1‑100 (MQ=255)                                                                     gatgatGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCggg                         <  2:861342/100‑1 (MQ=255)                                                                     gatgatGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCggg                         <  1:1921991/100‑1 (MQ=255)                                                                        gatgCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAAt                      <  1:1796426/100‑1 (MQ=255)                                                                         atgCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATg                     <  2:892189/100‑1 (MQ=255)                                                                         atgCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATg                     <  1:797511/100‑1 (MQ=255)                                                                            cATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGaaa                  <  1:1272063/100‑1 (MQ=255)                                                                              tGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAAcc                >  2:349849/1‑100 (MQ=255)                                                                               gTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAAccc               <  2:1891254/100‑1 (MQ=255)                                                                               gTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAAccc               >  2:1439655/1‑100 (MQ=255)                                                                               gTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATGAAAATAGTGAGTCCGGGAATGAAAccc               <  1:1041017/100‑1 (MQ=255)                                                                                 cGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCgg             >  2:742152/1‑100 (MQ=255)                                                                                 cGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCgg             >  2:2445284/1‑100 (MQ=255)                                                                                   ggATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGAc           <  1:341597/100‑1 (MQ=255)                                                                                   ggATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGAc           >  2:1028658/1‑100 (MQ=255)                                                                                   ggATTATTGAGATGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTCAAATAGTGAGTCCGGGAATGAAAccc               >  1:1925746/1‑96 (MQ=255)                                                                                    gATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGAcc          >  1:2422989/1‑100 (MQ=255)                                                                                    gATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGAcc          <  1:1337788/100‑1 (MQ=255)                                                                                    gATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGAcc          <  1:2592885/100‑1 (MQ=255)                                                                                    gATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGAcc          >  1:697411/1‑100 (MQ=255)                                                                                     attattGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCt         >  1:1222414/1‑100 (MQ=255)                                                                                     attattGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCt         >  2:195336/1‑100 (MQ=255)                                                                                      ttattGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTg        >  1:2228420/1‑100 (MQ=255)                                                                                         ttGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAAc     <  2:1999158/100‑1 (MQ=255)                                                                                         ttGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACACGGACCTGAAc     >  2:1034791/1‑100 (MQ=255)                                                                                           gAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTTAGTCCGGGAATGAAACCCGGACCTGAACAt   <  2:2406891/100‑1 (MQ=255)                                                                                           gAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACAt   <  1:679901/100‑1 (MQ=255)                                                                                            aGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATg  <  1:1485922/100‑1 (MQ=255)                                                                                                |                                                                                                CAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATG  >  NC_000913/715754‑715944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑