Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	741,817	C→T	20.4%	G247D (GGC→GAC)	dtpD ←	dipeptide and tripeptide permease D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	741,817	0	C	T	20.4%	200.7 / 42.2	98	G247D (GGC→GAC)	dtpD	dipeptide and tripeptide permease D
Reads supporting (aligned to +/- strand):  ref base C (35/43);  new base T (9/11);  total (44/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

ACAACATACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACCAGCAGCCA  >  NC_000913/741719‑741914                                                                                                   |                                                                                                  acaacaTACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGcc                                                                                                  <  2:873441/100‑1 (MQ=255)     caTACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCaata                                                                                              <  1:2073556/100‑1 (MQ=255)     caTACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCaata                                                                                              <  1:1327049/100‑1 (MQ=255)     caTACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCaata                                                                                              <  2:134076/100‑1 (MQ=255)      aTACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTTCCAGTACACCCAGGCCaataa                                                                                             >  2:1679157/1‑100 (MQ=255)      aTACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCaataa                                                                                             <  2:958004/100‑1 (MQ=255)      aTACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCaataa                                                                                             <  1:2127525/100‑1 (MQ=255)      aTACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCaataa                                                                                             <  1:204910/100‑1 (MQ=255)      aTACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCaata                                                                                              <  1:2520532/99‑1 (MQ=255)       tACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCaataat                                                                                            >  1:2293294/1‑100 (MQ=255)        aCTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGTCTTTTCTCCCAGGCCAataata                                                                                           >  1:621855/1‑100 (MQ=255)         cTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAg                                                                                          >  1:2259024/1‑100 (MQ=255)         cTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAg                                                                                          >  2:1009843/1‑100 (MQ=255)          tGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGt                                                                                         >  2:1626197/1‑100 (MQ=255)            aagaagGTGAGCGTCACAATCTGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTcg                                                                                       <  1:1518035/100‑1 (MQ=255)                agGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGAc                                                                                   >  2:1986229/1‑100 (MQ=255)                agGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGGTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGAc                                                                                   <  1:963632/100‑1 (MQ=255)                    gAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTTCCATTCTACCCAGGCCAATAATAGTCGCGACa                                                                                  >  1:324495/1‑97 (MQ=255)                      gCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTaa                                                                             <  1:307980/100‑1 (MQ=255)                        gTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAgg                                                                           <  1:81786/100‑1 (MQ=255)                         tCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGc                                                                          >  1:1805059/1‑100 (MQ=255)                          cacaATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCg                                                                         >  2:286978/1‑100 (MQ=255)                          cacaATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCg                                                                         <  2:853803/100‑1 (MQ=255)                            caATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGta                                                                       >  1:1049471/1‑100 (MQ=255)                              aTCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGtata                                                                     <  1:166958/100‑1 (MQ=255)                                cAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATAcc                                                                   >  2:1190345/1‑100 (MQ=255)                                  gCCCCAGCTCCTTCCGCTGTTGCTGGTGTTCTGCTTTGCGATAAAGTGTTGCCAGTACACCCAGGCGAGTAATAGTCGCGACATTTAAGGCGTATACCGa                                                                 >  1:1509369/1‑100 (MQ=255)                                   ccccAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGAc                                                                <  2:1612360/100‑1 (MQ=255)                                    cccAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGAcc                                                               <  1:2550178/100‑1 (MQ=255)                                    cccAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGAcc                                                               <  1:1327109/100‑1 (MQ=255)                                    cccAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGAcc                                                               <  2:137489/100‑1 (MQ=255)                                      cAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCAc                                                             >  1:516891/1‑100 (MQ=255)                                           ccTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCttt                                                        <  1:2540752/100‑1 (MQ=255)                                              tCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTTTTTTTTTTTTCAGGCCAATAATAGTCGCGACAATTAACTCGTATACCGACCACTCTTTCCa                                                     >  1:398196/1‑100 (MQ=255)                                              tCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCa                                                     >  2:460026/1‑100 (MQ=255)                                               ccGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAg                                                    <  2:711079/100‑1 (MQ=255)                                                  cTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAAc                                                 <  2:2220693/100‑1 (MQ=255)                                                  cTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAAc                                                 <  1:568807/100‑1 (MQ=255)                                                    gTTTCTGGCGTTCTGCTTTGCGATCACTTTTTGCCAGTACACCCAGGCCAATAATCGTCGCGACCATGACTGCTTATACCGACCACTCCTTCCAg                                                    <  1:1587509/95‑1 (MQ=255)                                                       ttttggtttCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTAt                                            <  1:2155960/94‑1 (MQ=255)                                                       tCTGGTTTTCTTCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTAt                                            <  1:2153440/100‑1 (MQ=255)                                                       tCTGGTTTTCTGCTTTGCGATAAATTTTTTTTTTTTTTTTCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACATTa                                             >  1:1769461/1‑99 (MQ=255)                                                        cTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATg                                           >  2:730929/1‑100 (MQ=255)                                                        cTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATg                                           >  1:743033/1‑100 (MQ=255)                                                         tGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATgg                                          <  2:1211112/100‑1 (MQ=255)                                                          ggTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGTTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGt                                         <  1:2512848/100‑1 (MQ=255)                                                          ggTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGt                                         <  2:1173756/100‑1 (MQ=255)                                                          ggTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGt                                         <  2:890750/100‑1 (MQ=255)                                                          ggTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGt                                         >  2:1020169/1‑100 (MQ=255)                                                           gTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTa                                        <  2:2501307/100‑1 (MQ=255)                                                             tttCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACGACTCTTTCCAGAACAGTATgg                                          >  1:2225753/1‑96 (MQ=255)                                                              ttCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATc                                     <  1:1240724/100‑1 (MQ=255)                                                              ttCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATc                                     <  2:1102335/100‑1 (MQ=255)                                                                  gCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTg                                 <  2:2066305/100‑1 (MQ=255)                                                                      tGCGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAgg                             <  1:574637/100‑1 (MQ=255)                                                                      tGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTACGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAgg                             <  2:1921990/100‑1 (MQ=255)                                                                       gCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGTGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGc                            >  1:723605/1‑100 (MQ=255)                                                                        cGATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCg                           >  1:2541429/1‑100 (MQ=255)                                                                        cGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAATGCAGGCg                           >  1:782391/1‑100 (MQ=255)                                                                         gATAAATTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCg                           >  1:938697/1‑99 (MQ=255)                                                                         gATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGt                          >  2:1201521/1‑100 (MQ=255)                                                                         gATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGt                          >  1:1617840/1‑100 (MQ=255)                                                                         gATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGt                          >  1:482874/1‑100 (MQ=255)                                                                          aTAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGtt                         >  2:2409410/1‑100 (MQ=255)                                                                          aTAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGtt                         >  2:1413885/1‑100 (MQ=255)                                                                           tAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACATTATGGTAATCAGTGCAGGCGTTg                        >  2:2107794/1‑100 (MQ=255)                                                                           tAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTg                        >  2:1230747/1‑100 (MQ=255)                                                                            aaaTTTTTGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGc                       >  2:394122/1‑100 (MQ=255)                                                                            aaaTTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGc                       <  2:2517765/100‑1 (MQ=255)                                                                              aTTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGcca                     >  2:2438402/1‑100 (MQ=255)                                                                              aTTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGcca                     >  2:1952771/1‑100 (MQ=255)                                                                              aTTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGcca                     <  2:786525/100‑1 (MQ=255)                                                                               tttttGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGccac                    <  2:1405068/100‑1 (MQ=255)                                                                                ttttGCCAGTACACCCATGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGcca                     <  1:795650/98‑1 (MQ=255)                                                                                ttttGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGccacc                   <  1:485857/100‑1 (MQ=255)                                                                                ttttGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGccacc                   <  2:1907230/100‑1 (MQ=255)                                                                                ttttGCCAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGccacc                   <  2:250560/100‑1 (MQ=255)                                                                                   tGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCTAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACcagc                <  1:1212876/100‑1 (MQ=255)                                                                                    gCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCTCTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACcagca               >  1:1871574/1‑100 (MQ=255)                                                                                     ccAGTACACCCAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACcagcag              >  1:725067/1‑100 (MQ=255)                                                                                      cAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGa             <  1:2251347/100‑1 (MQ=255)                                                                                       aGTACACCCAGGTCAATAATAGGCGCGACAATTAAGGCGTATAGCGACCACTCTTGCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGaa            <  2:760003/100‑1 (MQ=255)                                                                                       aGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGaa            >  1:2012103/1‑100 (MQ=255)                                                                                       aGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTAGACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGAGTTGCCACCAGCAGaa            >  1:1412617/1‑100 (MQ=255)                                                                                          acacCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACca         <  1:1059306/100‑1 (MQ=255)                                                                                           cacCCAGGCCAATAATAGTCGCGACAATTATGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACcag        <  1:548230/100‑1 (MQ=255)                                                                                           cacCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACcag        >  1:1312139/1‑100 (MQ=255)                                                                                           cacCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACcag        <  2:1278652/100‑1 (MQ=255)                                                                                           cacCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACcag        >  1:1312190/1‑100 (MQ=255)                                                                                           cacCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACcag        <  2:2228167/100‑1 (MQ=255)                                                                                            acCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACcagc       >  1:1858607/1‑100 (MQ=255)                                                                                             cccAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACcagca      >  1:1558765/1‑100 (MQ=255)                                                                                             cccAGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACcagca      >  1:1558084/1‑100 (MQ=255)                                                                                              ccAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACcagcag     <  1:1454769/100‑1 (MQ=255)                                                                                               cAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACCagcagc    <  2:2292026/100‑1 (MQ=255)                                                                                               cAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACCagcagc    <  2:2292018/100‑1 (MQ=255)                                                                                                aGGTCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACCAGCAGcc   <  1:740174/100‑1 (MQ=255)                                                                                                aGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACCAGCAGcc   <  1:168505/100‑1 (MQ=255)                                                                                                 ggCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACCAGCAGCCa  <  1:1952761/100‑1 (MQ=255)                                                                                                   |                                                                                                  ACAACATACTGAAGAAGGTGAGCGTCACAATCAGCCCCAGCTCCTTCCGCTGTTTCTGGTTTTCTGCTTTGCGATAAATTTTTGCCAGTACACCCAGGCCAATAATAGTCGCGACAATTAAGGCGTATACCGACCACTCTTTCCAGAACAGTATGGTAATCAGTGCAGGCGTTGCCACCAGCAGAACCAGCAGCCA  >  NC_000913/741719‑741914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑