Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 975,700 G→T 16.7% D27Y (GAC→TAC) ‡ mukE → chromosome condensin MukBEF, MukE localization factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913975,7000GT16.7% 253.4 / 32.2 94D27Y (GAC→TAC) mukEchromosome condensin MukBEF, MukE localization factor
Reads supporting (aligned to +/- strand):  ref base G (40/38);  new base T (9/7);  total (49/45)
Fisher's exact test for biased strand distribution p-value = 7.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

ACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGC  >  NC_000913/975601‑975799
                                                                                                   |                                                                                                   
aCGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTgg                                                                                                     >  2:641668/1‑100 (MQ=255)
 cGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGa                                                                                                    >  1:1003156/1‑100 (MQ=255)
  ggAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGAc                                                                                                   <  2:1736826/100‑1 (MQ=255)
    aGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAg                                                                                                 <  2:1826036/100‑1 (MQ=255)
    aGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAg                                                                                                 <  1:365336/100‑1 (MQ=255)
    aGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAg                                                                                                 <  1:950923/100‑1 (MQ=255)
     gCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAgc                                                                                                <  2:1266028/100‑1 (MQ=255)
     gCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAgc                                                                                                >  1:1083344/1‑100 (MQ=255)
      ccAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAgcg                                                                                               >  2:1977913/1‑100 (MQ=255)
       cAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAgcgc                                                                                              >  1:1729689/1‑100 (MQ=255)
        aaGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGcc                                                                                             <  1:2406183/100‑1 (MQ=255)
         aGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCTGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCt                                                                                            <  1:1652012/100‑1 (MQ=255)
          ggTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGTCCAGCGccgg                                                                                           <  2:481894/100‑3 (MQ=255)
          ggTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGTCCAGCGccgg                                                                                           <  1:787514/100‑3 (MQ=255)
          ggTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGTCCAGCGccgg                                                                                           <  2:1148729/100‑3 (MQ=255)
          ggTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGTCCAGCGccgg                                                                                           <  2:378128/100‑3 (MQ=255)
          ggTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCtt                                                                                           <  1:641665/100‑1 (MQ=255)
          ggTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCtt                                                                                           >  1:2583762/1‑100 (MQ=255)
           gTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTa                                                                                          <  2:50980/100‑1 (MQ=255)
           gTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTa                                                                                          >  1:2128657/1‑100 (MQ=255)
             aCAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGTCCAGCGccggaaa                                                                                        <  1:781754/100‑6 (MQ=255)
             aCAGGCGCATGTCAACGACAAATCTTGCACAAGAGATGCCGGTTAAGCTCACGCAGGCACTGGCTAATCCGCTATTTCCGGCGCTGGACAGCGCCTTACg                                                                                        >  1:1839640/1‑100 (MQ=255)
                ggCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTc                                                                                     >  2:1991380/1‑100 (MQ=255)
                ggCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTc                                                                                     >  2:1555623/1‑100 (MQ=255)
                ggCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTc                                                                                     <  2:395422/100‑1 (MQ=255)
                ggCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTc                                                                                     >  1:484573/1‑100 (MQ=255)
                ggCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTc                                                                                     >  1:1300549/1‑100 (MQ=255)
                 gcggATGTCATTGACAAATATTGACCAAGTGACGCCGGTTAAGTTGGCGCAGTGGCTGGCGAATCCGTTATTTCCGGCGCTGTCCAGCGcc                                                                                             <  1:2096951/91‑1 (MQ=255)
                  cgcATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGTCCAGCGccggaaataacg                                                                                   <  1:752024/100‑11 (MQ=38)
                  cgcATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAg                                                                                   >  1:776173/1‑100 (MQ=255)
                   gcATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAgg                                                                                  <  1:2549132/100‑1 (MQ=255)
                      tGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACg                                                                               >  1:89701/1‑100 (MQ=255)
                               cAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTgg                                                                      >  2:42207/1‑100 (MQ=255)
                                 aaTATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGcc                                                                    >  2:1636562/1‑100 (MQ=255)
                                 aaTATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGcc                                                                    <  2:561330/100‑1 (MQ=255)
                                 aaTATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGcc                                                                    <  1:486840/100‑1 (MQ=255)
                                   tatTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACTCCATATTGGCCTc                                                                  <  2:1743336/100‑1 (MQ=255)
                                    atTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTcg                                                                 >  2:1017240/1‑100 (MQ=255)
                                    atTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTcg                                                                 >  2:620645/1‑100 (MQ=255)
                                       gAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTcgccg                                                              >  2:2115489/1‑100 (MQ=255)
                                        aaCAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTcgacga                                                             >  2:555453/1‑100 (MQ=255)
                                         aCAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGaa                                                            >  1:296842/1‑100 (MQ=255)
                                          cAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAAc                                                           <  1:2259764/100‑1 (MQ=255)
                                           aaGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACt                                                          <  2:354186/100‑1 (MQ=255)
                                           aaGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACt                                                          <  1:1459068/100‑1 (MQ=255)
                                            aGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTg                                                         <  2:1036447/100‑1 (MQ=255)
                                                 tGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATaa                                                    >  2:1538872/1‑100 (MQ=255)
                                                 tGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATaa                                                    >  2:904958/1‑100 (MQ=255)
                                                 tGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATaa                                                    <  1:2429586/100‑1 (MQ=255)
                                                 tGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATa                                                     <  1:2274633/99‑1 (MQ=255)
                                                  gCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATaan                                                   <  1:904955/100‑2 (MQ=255)
                                                   ccGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATc                                                  <  1:2454381/100‑1 (MQ=255)
                                                    cGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCa                                                 <  2:1630335/100‑1 (MQ=255)
                                                     ggTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCAt                                                >  2:1810093/1‑100 (MQ=255)
                                                      gTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATg                                               <  1:2009218/100‑1 (MQ=255)
                                                       ttAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGc                                              <  1:1092376/100‑1 (MQ=255)
                                                           gCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTc                                          >  1:1423418/1‑100 (MQ=255)
                                                           gCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTc                                          >  1:427060/1‑100 (MQ=255)
                                                           gCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTc                                          >  1:10836/1‑100 (MQ=255)
                                                            ntGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTcc                                         >  1:1195645/2‑100 (MQ=255)
                                                            cTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACTCCATATTGGCCTCGACGAACTGGATAATCATGCATTcc                                         <  1:1591449/100‑1 (MQ=255)
                                                            cTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTcc                                         <  1:334571/100‑1 (MQ=255)
                                                                cgcAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGAt                                     >  2:402238/1‑100 (MQ=255)
                                                                cgcAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGAt                                     >  1:1211100/1‑100 (MQ=255)
                                                                 gcAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATg                                    <  1:1942881/100‑1 (MQ=255)
                                                                    ggCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGAt                                 >  1:1397676/1‑100 (MQ=255)
                                                                          ggCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCa                            >  2:2063889/1‑99 (MQ=255)
                                                                           tccgatctgTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAgg                          <  1:1248045/92‑1 (MQ=255)
                                                                           gCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAgg                          <  2:1423426/100‑1 (MQ=255)
                                                                           gCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAgg                          <  1:892462/100‑1 (MQ=255)
                                                                           gCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAgg                          <  2:2354969/100‑1 (MQ=255)
                                                                            cGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGa                         <  2:711927/100‑1 (MQ=255)
                                                                                 ccGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATc                    >  1:2587810/1‑100 (MQ=255)
                                                                                  ctgaacgtaaggCGCTGTCCAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCt                   >  1:1804175/11‑100 (MQ=38)
                                                                                  cGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCt                   >  2:1956742/1‑100 (MQ=255)
                                                                                  cGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCt                   >  2:824271/1‑100 (MQ=255)
                                                                                   gTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGagatcgga                  >  2:1248049/1‑93 (MQ=255)
                                                                                     tATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGa                <  2:2514790/100‑1 (MQ=255)
                                                                                     tATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGa                <  2:2474461/100‑1 (MQ=255)
                                                                                      acgtaaggCGCTGTCCAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGaa               >  1:2546027/7‑100 (MQ=255)
                                                                                      acgtaaggCGCTGTCCAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGaa               >  2:550154/7‑100 (MQ=255)
                                                                                       tttCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGTATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAag              <  1:142848/100‑1 (MQ=255)
                                                                                       tttCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAag              <  2:2030190/100‑1 (MQ=255)
                                                                                       ngtaaggCGCTGTCCAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAag              >  1:647785/6‑100 (MQ=255)
                                                                                       cgtaaggCGCTGTCCAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTACAGGAATATCTggc                >  1:1525288/6‑97 (MQ=255)
                                                                                         taaggCGCTGTCCAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAagag            >  2:111690/4‑100 (MQ=255)
                                                                                         taaggCGCTGTCCAGCGCCTTACGTTCAGGACGACATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAagag            >  1:1455064/4‑100 (MQ=255)
                                                                                         tCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAagag            >  1:1062894/1‑100 (MQ=255)
                                                                                          ccGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGt           >  2:2243136/1‑100 (MQ=255)
                                                                                          aaggCGCTGTCCAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAagag            >  2:1414162/3‑99 (MQ=255)
                                                                                           cGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAag              >  1:1422218/1‑96 (MQ=255)
                                                                                           cGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGtt          <  2:249825/100‑1 (MQ=255)
                                                                                           cGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGtt          >  2:1701279/1‑100 (MQ=255)
                                                                                            ggCGCTGTCCAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGttt         >  2:2465879/1‑100 (MQ=255)
                                                                                                cTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGACTATCTGGAAGAGTTTTAc      >  1:485667/1‑99 (MQ=255)
                                                                                                   gACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAcgcgc  <  1:377521/100‑1 (MQ=255)
                                                                                                   gACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTAcgcgc  >  1:1028006/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
ACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATATTGAACAAGTGATGCCGGTTAAGCTGGCGCAGGCGCTGGCGAATCCGTTATTTCCGGCGCTGGACAGCGCCTTACGTTCAGGACGCCATATTGGCCTCGACGAACTGGATAATCATGCATTCCTGATGGATTTTCAGGAATATCTGGAAGAGTTTTACGCGC  >  NC_000913/975601‑975799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: