Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,018,256	G→C	15.2%	T15S (ACC→AGC)	ycbZ ←	putative peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,018,256	0	G	C	15.2%	249.5 / 36.3	92	T15S (ACC→AGC)	ycbZ	putative peptidase
Reads supporting (aligned to +/- strand):  ref base G (42/36);  new base C (7/7);  total (49/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.66e-01

TGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAACCGGCACTAC  >  NC_000913/1018157‑1018355                                                                                                    |                                                                                                    tGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCgg                                                                                                     <  1:2517977/100‑1 (MQ=255) tGCTCCAGCGCAAATGGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCgg                                                                                                     <  1:496172/100‑1 (MQ=255)  gCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGt                                                                                                    >  2:1302561/1‑100 (MQ=255)  gCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCATCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGt                                                                                                    >  1:699233/1‑100 (MQ=255)   cTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGCTAGCTATCGGTa                                                                                                   >  1:2153624/1‑100 (MQ=255)    tCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTAt                                                                                                  >  1:2007481/1‑100 (MQ=255)    tCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTAt                                                                                                  >  1:2585205/1‑100 (MQ=255)     ccAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATc                                                                                                 <  1:1427923/100‑1 (MQ=255)      cAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCa                                                                                                <  2:1306325/100‑1 (MQ=255)       aGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAg                                                                                               >  2:196463/1‑100 (MQ=255)       aGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAg                                                                                               <  2:2585224/100‑1 (MQ=255)        gcgcAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATACCGATAGCTATCAgg                                                                                              <  1:725338/100‑1 (MQ=255)         cgcAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGa                                                                                             >  1:324392/1‑100 (MQ=255)         cgcAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATACCGATAGCTATCAGGa                                                                                             <  1:488168/100‑1 (MQ=255)         cgcAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATACCGATAGCTATCAGGa                                                                                             <  1:1145464/100‑1 (MQ=255)          gcAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATACCGATAGCTATCAGGaa                                                                                            <  2:1003529/100‑1 (MQ=38)          gcAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATACCGATAGCTATCAGGaa                                                                                            <  1:1303945/100‑1 (MQ=38)               ttGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATCTTTCCTGATAGCTATCGGTATGAGGAACCa                                                                                         >  1:1234332/1‑98 (MQ=255)                tGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGt                                                                                      >  1:1296128/1‑100 (MQ=255)                tGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGt                                                                                      >  2:2586244/1‑100 (MQ=255)                 gCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGTGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTc                                                                                     <  1:1412992/100‑1 (MQ=255)                 gCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTc                                                                                     >  1:1262476/1‑100 (MQ=255)                  cAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCa                                                                                    >  2:984419/1‑100 (MQ=255)                  cAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATACCGATAGCTATCAGGaaatatttgc                                                                                    <  2:2567949/100‑9 (MQ=25)                   aGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATACCGATAGCTATCAGGaaatatt                                                                                       <  2:954033/96‑6 (MQ=37)                    gCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACg                                                                                  <  1:984416/100‑1 (MQ=255)                     ccGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGc                                                                                 >  2:862031/1‑100 (MQ=255)                     ccGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGc                                                                                 <  2:2291044/100‑1 (MQ=255)                      cgcgGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGcc                                                                                >  2:1370070/1‑100 (MQ=255)                         ggatGAGTATCACTGAATAAAGGATCGTTTTCGCCAATCAAATCTGCCTGACCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATg                                                                             <  2:117366/97‑1 (MQ=255)                         ggTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATg                                                                             >  2:529546/1‑100 (MQ=255)                         ggTGGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATg                                                                             <  1:2359196/100‑1 (MQ=255)                               gTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGtt                                                                       <  1:352859/100‑1 (MQ=255)                                 aTCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTc                                                                     >  1:2138317/1‑100 (MQ=255)                                 aTCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTc                                                                     <  2:1163062/100‑1 (MQ=255)                                  tCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCg                                                                    >  2:1894842/1‑100 (MQ=255)                                  tCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCg                                                                    <  2:1289186/100‑1 (MQ=255)                                  tCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCg                                                                    <  1:1143892/100‑1 (MQ=255)                                    aCTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTa                                                                  >  2:307122/1‑100 (MQ=255)                                     cTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTaa                                                                 >  2:2347355/1‑100 (MQ=255)                                       gAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATg                                                               >  1:1844837/1‑100 (MQ=255)                                         aTAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGt                                                             >  2:2511688/1‑100 (MQ=255)                                              ggATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAg                                                        >  2:1483455/1‑100 (MQ=255)                                                 tCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTg                                                     >  2:1891298/1‑100 (MQ=255)                                                 tCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTg                                                     >  2:958638/1‑100 (MQ=255)                                                  cGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGa                                                    >  1:912187/1‑100 (MQ=255)                                                   gTTTTTGTGAATCAAATGTGGCTGGGCAAAGATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGAt                                                   <  1:1766898/100‑1 (MQ=255)                                                   gTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGAt                                                   <  1:1767290/100‑1 (MQ=255)                                                    ttttCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACAAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATg                                                  >  2:1324712/1‑100 (MQ=255)                                                    ttttCGTCAATCAAAGGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGAtn                                                  <  1:2089328/100‑2 (MQ=255)                                                        cGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGtttt                                              >  2:2004039/1‑100 (MQ=255)                                                          tcaatcaaATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGtttttt                                            <  2:721434/100‑1 (MQ=255)                                                          tcaatcaaATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGtttttt                                            >  2:2312976/1‑100 (MQ=255)                                                           caatcaaATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTa                                           >  2:1229254/1‑100 (MQ=255)                                                           caatcaaATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTa                                           >  2:2279612/1‑100 (MQ=255)                                                            aatcaaATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAg                                          >  2:2061878/1‑100 (MQ=255)                                                              tcaaATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTc                                        >  2:1760707/1‑100 (MQ=255)                                                                aaaTGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCtg                                      >  2:949664/1‑100 (MQ=255)                                                                 aaTGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCtgt                                     <  1:1575567/100‑1 (MQ=255)                                                                    gtgGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCtgttgt                                  >  2:162510/1‑100 (MQ=255)                                                                     tgGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAATTTGATGTTTTTTAGTCTGTTGTc                                 <  2:442275/100‑1 (MQ=255)                                                                      ggCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCa                                <  2:2579171/100‑1 (MQ=255)                                                                      ggCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCa                                <  2:105030/100‑1 (MQ=255)                                                                          gAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGc                            <  2:1534015/100‑1 (MQ=255)                                                                          gAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGc                            <  2:2145233/100‑1 (MQ=255)                                                                          gAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGc                            <  1:2483720/100‑1 (MQ=255)                                                                          gAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAAGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGc                            >  2:2480009/1‑100 (MQ=255)                                                                          gAGCAAATATTTCCTGATAGCTATCGGTAGCAGGAACCAGGTCACGCTATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGc                            <  1:1930912/100‑1 (MQ=255)                                                                             cAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCcgc                         >  2:301393/1‑100 (MQ=255)                                                                              acctggttCCTGATACCGATAGCTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCcgcg                        >  1:231938/7‑100 (MQ=37)                                                                               aaTATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCcgcg                        <  1:724366/99‑1 (MQ=255)                                                                               aaTATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGa                       <  1:856925/100‑1 (MQ=255)                                                                                ctggttCCTGATACCGATAGCTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGAt                      >  2:479611/5‑100 (MQ=37)                                                                                  ggttCCTGATACCGATAGCTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATta                    >  1:98437/3‑100 (MQ=38)                                                                                   gttCCTGATACCGATAGCTATCAGGAACCAGGTCACGCCATGCAAGTTTTGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATtat                   >  1:872584/2‑100 (MQ=38)                                                                                   gttCCTGATACCGATAGCTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATtat                   >  1:932638/2‑100 (MQ=38)                                                                                   gttCCTGATACCGATAGCTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATtat                   >  1:1827900/2‑100 (MQ=38)                                                                                    ttCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATtata                  <  2:329941/100‑1 (MQ=255)                                                                                     tCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATAc                 <  1:2342557/100‑1 (MQ=255)                                                                                     tCCTGATACCGATAGCTATCAGGAACCAGGTCACGCCATGCCAGTTTCGTACTGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATAc                 >  1:583111/1‑100 (MQ=255)                                                                                      ccTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATAcc                >  1:1734368/1‑100 (MQ=255)                                                                                       cTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCg               <  2:699236/100‑1 (MQ=255)                                                                                       cTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCg               <  1:1041925/100‑1 (MQ=255)                                                                                       cTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCg               >  1:1978414/1‑100 (MQ=255)                                                                                          aTAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTaa            <  2:10758/100‑1 (MQ=255)                                                                                             gCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAACCg         >  1:1473251/1‑100 (MQ=255)                                                                                             gCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAACCg         >  2:1601318/1‑100 (MQ=255)                                                                                             gCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAACCg         <  1:1788114/100‑1 (MQ=255)                                                                                               tATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAACCGGc       >  2:1230154/1‑100 (MQ=255)                                                                                               tATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAACCGGc       <  2:1003487/100‑1 (MQ=255)                                                                                                aTCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAACCGGCa      <  1:46912/100‑1 (MQ=255)                                                                                                 tCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAACCGGCAc     >  2:297553/1‑100 (MQ=255)                                                                                                 tCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAACCGGCAc     <  1:226192/100‑1 (MQ=255)                                                                                                  cGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAACCGGCACt    <  1:1403698/100‑1 (MQ=255)                                                                                                    gTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAACCGGCACTAc  >  2:1519296/1‑100 (MQ=255)                                                                                                    |                                                                                                    TGCTCCAGCGCAAATTGCAGCCGCGGTTGAGTATCACTGAATAAAGGATCGTTTTCGTCAATCAAATGTGGCTGAGCAAATATTTCCTGATAGCTATCGGTATCAGGAACCAGGTCACGCCATGCAAGTTTCGTAATGGTCAAAGTTGATGTTTTTTAGTCTGTTGTCAAAGCCGCGATTATACCGTAACCGGCACTAC  >  NC_000913/1018157‑1018355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑