Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	420,149	Δ1 bp	coding (559/1320 nt)	brnQ →	branched‑chain amino acid transport system 2 carrier protein; LIV‑II transport system for Ile, Leu, and Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	420,146	0	T	.	98.8%	363.2 / ‑2.5	83	coding (556/1320 nt)	brnQ	branched‑chain amino acid transport system 2 carrier protein; LIV‑II transport system for Ile, Leu, and Val
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base . (38/44);  total (38/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

CGCTGAAAATTATCGCGCTGGTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGC  >  NC_000913/420048‑420241                                                                                                   |                                                                                                cgcTGAAAATTATCGCGCTGGTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGtt                                                                                                <  2:454466/100‑1 (MQ=255)  gcTGAAAATTATCGCGCTGGTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGttt                                                                                               >  2:2806306/1‑100 (MQ=255)   cTGAAAATTATCGCGCTGGTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGtttc                                                                                              >  2:2358875/1‑99 (MQ=255)    tGAAAATTATCGCGCTGGTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGtttct                                                                                             <  2:134180/100‑3 (MQ=255)    tGAAAATTATCGCGCTGGTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGtttct                                                                                             >  1:218549/1‑98 (MQ=255)     gAAAATTATCGCGCTGGTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCCCGGCGACTGAGGCTTATCAAAACGCTGCGtttcta                                                                                            >  1:27666/1‑97 (MQ=255)     gAAAATTATCGCGCTGGTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGtttcta                                                                                            <  1:2256236/100‑4 (MQ=255)     gAAAATTATCGCGCTGGTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGAGtttcta                                                                                            <  1:1525052/100‑4 (MQ=255)        aaTTATCGCGCTGGTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACg                                                                                        >  2:181490/1‑100 (MQ=255)        aaTTATCGCGCTGGTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACg                                                                                        >  1:1478152/1‑100 (MQ=255)        aaTTATCGCGCTGGTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACg                                                                                        <  2:1625528/100‑1 (MQ=255)        aaTTATCGCGCTGGTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACg                                                                                        <  1:2349519/100‑1 (MQ=255)          ttATCGCGCTGGTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGc                                                                                      <  1:2274481/100‑1 (MQ=255)          ttATCGCGCTGGTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGc                                                                                      >  1:2565558/1‑100 (MQ=255)             tCGCGCTGGTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACAGCTTc                                                                                   <  2:340581/100‑1 (MQ=255)              cgcgCTGGTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCg                                                                                  <  2:2864164/100‑1 (MQ=255)              cgcgCTGGTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCg                                                                                  <  2:2551510/100‑1 (MQ=255)               gcgcTGGTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCg                                                                                  <  2:529409/100‑1 (MQ=255)                   tGGTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGCGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTacc                                                                             >  2:224427/1‑98 (MQ=255)                    ggTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACg                                                                            >  1:2101424/1‑100 (MQ=255)                      tCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACg                                                                            <  1:1065013/98‑1 (MQ=255)                       cATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCt                                                                         >  2:785604/1‑100 (MQ=255)                          ccTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATc                                                                      <  2:2797932/100‑1 (MQ=255)                          ccTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATc                                                                      <  2:44819/100‑1 (MQ=255)                             gtctgtTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGa                                                                   <  1:1420882/100‑1 (MQ=255)                             gtctgtTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGa                                                                   <  1:1856169/100‑1 (MQ=255)                               ctgtTGCCGCTATTGTCTGGCCGGCGGGATTTATCTGCACGGCGACTGCGGATTATAAAAACGATGAG‑TTTCTAACGGCTTCGTTCACCGCTCTCTGAc                                                                  >  2:270587/1‑99 (MQ=255)                                tgtTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCa                                                                >  1:2215365/1‑100 (MQ=255)                                tgtTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCa                                                                >  2:2278646/1‑100 (MQ=255)                                 gtTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCAt                                                               >  1:742486/1‑100 (MQ=255)                                  ttGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGCCTATCTGACCATg                                                              <  2:1787405/100‑1 (MQ=255)                                    gCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGa                                                            >  1:795192/1‑100 (MQ=255)                                     ccGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGAt                                                           >  1:2397462/1‑100 (MQ=255)                                     ccGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGAt                                                           >  1:2868142/1‑100 (MQ=255)                                      cGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATa                                                          <  1:1092252/100‑1 (MQ=255)                                       gCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCCAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATAc                                                         >  2:400310/1‑100 (MQ=255)                                       gCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATAc                                                         <  1:2277081/100‑1 (MQ=255)                                        cTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACg                                                        >  1:2347828/1‑100 (MQ=255)                                         tATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGc                                                       <  2:264248/100‑1 (MQ=255)                                          aTTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCt                                                      >  1:2287025/1‑100 (MQ=255)                                             gTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTggg                                                   >  2:427910/1‑100 (MQ=255)                                                tGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGgcgc                                                <  1:181491/100‑1 (MQ=255)                                                 ggCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCa                                               >  1:1603012/1‑100 (MQ=255)                                                  gCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCaa                                              >  2:1802872/1‑100 (MQ=255)                                                     ggcggGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATgg                                           >  2:137620/1‑100 (MQ=255)                                                     ggcggGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATgg                                           <  1:806538/100‑1 (MQ=255)                                                      gcggGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGgt                                          <  1:2520751/100‑1 (MQ=255)                                                      gcggGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGgt                                          <  2:2378906/100‑1 (MQ=255)                                                       tccgatCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGgtg                                         <  1:1455510/95‑1 (MQ=255)                                                        gggTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGgtgt                                        >  1:1677221/1‑100 (MQ=255)                                                         ggTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGtt                                       >  2:2879541/1‑100 (MQ=255)                                                         ggTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGtt                                       <  1:766124/100‑1 (MQ=255)                                                          gTTCTATCAGCACGGCGACTGCGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGCCCATGGATACGCTGGGCGCAATGGTGttt                                      >  2:709567/1‑100 (MQ=255)                                                          gTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGttt                                      <  2:1892678/100‑1 (MQ=255)                                                           ttCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTg                                     <  1:470313/100‑1 (MQ=255)                                                            tCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTACCTGACCATGGATACGCTGGGCGCAATGGTGTTTgg                                    >  2:507881/1‑100 (MQ=255)                                                             cTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGt                                   <  1:1539376/100‑1 (MQ=255)                                                             cTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGt                                   <  1:1537583/100‑1 (MQ=255)                                                               aTCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGgtgagatcgga                                 >  2:1455503/1‑92 (MQ=255)                                                               aTCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTAt                                 >  1:2164179/1‑100 (MQ=255)                                                               aTCAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAAGGGTGATTGGTAt                                 >  1:740141/1‑100 (MQ=255)                                                                 cAGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCg                               <  2:1124711/100‑1 (MQ=255)                                                                  aGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGt                              >  2:98634/1‑100 (MQ=255)                                                                  aGCACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGt                              >  1:2254549/1‑100 (MQ=255)                                                                    cACGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTa                            >  1:2135891/1‑100 (MQ=255)                                                                     aCGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTAt                           <  1:2879562/100‑1 (MQ=255)                                                                     aCGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTAt                           <  2:944328/100‑1 (MQ=255)                                                                      cGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTAtt                          >  2:2489721/1‑100 (MQ=255)                                                                      cGGCGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTAtt                          >  1:788109/1‑100 (MQ=255)                                                                         cGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGtt                       <  2:2143542/100‑1 (MQ=255)                                                                         cGACTGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGtt                       <  2:1360178/100‑1 (MQ=255)                                                                          gACTGAGGTTTATTAAAGCTCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATTTTACTATAGATATGCTGGGCGCCATGGTGTTTGGTATCGTTATTGtt                       <  2:1189931/99‑1 (MQ=255)                                                                             tGAGGCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAAcg                   <  1:1851550/100‑1 (MQ=255)                                                                                ggCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACgcgg                >  2:2520672/1‑100 (MQ=255)                                                                                ggCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTCGGCGCAATGGCGTTTGGTATCGTTATTGTTAACgcgg                >  2:2583908/1‑100 (MQ=255)                                                                                 gCTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACgcggc               <  1:785605/100‑1 (MQ=255)                                                                                  cTTATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACgcggcg              <  2:757767/100‑1 (MQ=255)                                                                                    tATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGcgcg            <  2:1000399/100‑1 (MQ=255)                                                                                    tATCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGcgcg            >  1:779135/1‑100 (MQ=255)                                                                                     aTCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGt           <  1:635797/100‑1 (MQ=255)                                                                                      tCAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGtt          >  2:2116076/1‑100 (MQ=255)                                                                                       cAAAACGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTtc         <  2:2215352/100‑1 (MQ=255)                                                                                        aaaaCGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTtct        >  2:955796/1‑100 (MQ=255)                                                                                          aaCGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCg      <  2:788108/100‑1 (MQ=255)                                                                                          aaCGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGCCGCGttaaca      <  1:2300615/100‑5 (MQ=255)                                                                                          aaCGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGCCGCGttaaca      <  2:795191/100‑5 (MQ=255)                                                                                            cGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGt     >  2:391867/1‑99 (MQ=255)                                                                                            cGCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGCCGCGttaacaat    <  1:2806327/100‑7 (MQ=255)                                                                                             gCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGCCGCGttaacaata   <  1:2366710/100‑8 (MQ=255)                                                                                             gCTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGCCGCGttaacaata   <  2:1478145/100‑8 (MQ=255)                                                                                              cTGCG‑TTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATTGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGc  <  1:1021856/100‑1 (MQ=255)                                                                                                   |                                                                                                CGCTGAAAATTATCGCGCTGGTCATCCTGTCTGTTGCCGCTATTGTCTGGCCGGCGGGTTCTATCAGCACGGCGACTGAGGCTTATCAAAACGCTGCGTTTTCTAACGGCTTCGTTAACGGCTATCTGACCATGGATACGCTGGGCGCAATGGTGTTTGGTATCGTTATTGTTAACGCGGCGCGTTCTCGTGGC  >  NC_000913/420048‑420241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑