BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,227,628	(A)_5→4	intergenic (+117/‑103)	metH → / → yjbB	homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent/putative Na+/Pi‑cotransporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,227,624	0	A	.	100.0%	564.3 / NA	126	intergenic (+113/‑107)	metH/yjbB	homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent/putative Na+/Pi‑cotransporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (69/57); total (69/57)

Only 100 of 131 total aligned reads displayed.

ACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGA  >  NC_000913/4227526‑4227718

aCTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGaa                                                                                               >  2:2442932/1‑100 (MQ=255)

   tttCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGaaaac                                                                                            >  2:2551551/1‑99 (MQ=255)

    ttCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGaaaacg                                                                                           <  1:253402/100‑3 (MQ=255)

     tCCTTACAACAGACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGaaaacga                                                                                          <  1:2365227/100‑4 (MQ=255)

       cTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAGACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAAt                                                                                       <  2:979596/100‑1 (MQ=255)

       cTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAAt                                                                                       <  1:300030/100‑1 (MQ=255)

       cTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAAt                                                                                       >  1:1887354/1‑100 (MQ=255)

        ttACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATg                                                                                      <  2:173739/100‑1 (MQ=255)

         tACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGt                                                                                     <  2:2198660/100‑1 (MQ=255)

         tACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGt                                                                                     <  2:862552/100‑1 (MQ=255)

         tACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGt                                                                                     >  2:1647628/1‑100 (MQ=255)

           caacaaACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCa                                                                                   >  1:2319921/1‑100 (MQ=255)

           caacaaACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAAGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCa                                                                                   >  1:410045/1‑100 (MQ=255)

            aagagacaGGGCACTCATTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAACCGAATGTCaa                                                                                  >  2:1675707/6‑100 (MQ=37)

            aagagacaGGGCACTCATTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCaa                                                                                  >  2:2669907/6‑100 (MQ=37)

            aacaaacaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCaa                                                                                  <  2:1464378/100‑1 (MQ=255)

             agagacaGGGCACTCATTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCaaa                                                                                 >  1:1111142/5‑100 (MQ=37)

             acaaacaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCaaa                                                                                 <  2:663928/100‑1 (MQ=255)

               aaacaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAg                                                                               <  2:505221/100‑1 (MQ=255)

               aaacaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAg                                                                               <  1:1360557/100‑1 (MQ=255)

                gacaGGGCACTCATTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCaaaa                                                                                >  2:2859703/2‑98 (MQ=38)

                gacaGGGCACTCATTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAgg                                                                              >  2:402735/2‑100 (MQ=38)

                 acaGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGc                                                                             <  1:1467798/100‑1 (MQ=255)

                  caGGGCACTCATTGAGCGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAAACTTAGCCGG‑AAAACGAATGTCAAAAGGca                                                                            >  2:1740930/1‑100 (MQ=255)

                    gggCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGcaca                                                                          >  2:1338820/1‑100 (MQ=255)

                        gcTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTaa                                                                      >  1:2617006/1‑100 (MQ=255)

                        gcTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTaa                                                                      >  1:1589541/1‑100 (MQ=255)

                        gcTCAATCAGTACCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTaa                                                                      >  2:1518494/1‑100 (MQ=255)

                         cTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTaaa                                                                     >  2:845096/1‑100 (MQ=255)

                           cAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGa                                                                   <  2:2616987/100‑1 (MQ=255)

                           cAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGa                                                                   >  1:1276918/1‑100 (MQ=255)

                           cAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGa                                                                   >  1:1391243/1‑100 (MQ=255)

                           cAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGa                                                                   >  2:2333427/1‑100 (MQ=255)

                           cAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGa                                                                   >  1:1507840/1‑100 (MQ=255)

                            aaTGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAg                                                                    <  2:2760256/98‑1 (MQ=255)

                             aTGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGCAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATg                                                                 <  2:1843458/100‑1 (MQ=255)

                             aTGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATg                                                                 <  1:2157833/100‑1 (MQ=255)

                              tGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGc                                                                >  1:906751/1‑100 (MQ=255)

                               gAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAATCAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGcc                                                               <  2:1844666/100‑1 (MQ=255)

                               gAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGcc                                                               <  1:1062839/100‑1 (MQ=255)

                                    cctttcatctTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAACGCACAGTAAAGATGcc                                                               <  2:1445224/88‑1 (MQ=255)

                                    cccTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCa                                                          >  2:1613901/1‑100 (MQ=255)

                                     ccTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAg                                                         >  2:2012753/1‑100 (MQ=255)

                                          ctctTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCgtgt                                                    >  1:932928/1‑100 (MQ=255)

                                           tctTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGtt                                                   >  2:188829/1‑100 (MQ=255)

                                           tctTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGtt                                                   >  2:1402363/1‑100 (MQ=255)

                                           tctTTATTAATATGAAACACGTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGtt                                                   >  2:1771688/1‑100 (MQ=255)

                                             tttATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTc                                                 >  1:345985/1‑100 (MQ=255)

                                             tttATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTc                                                 >  2:687036/1‑100 (MQ=255)

                                               tattaATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCg                                               >  1:785186/1‑100 (MQ=255)

                                                attaATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCgg                                              <  2:355225/100‑1 (MQ=255)

                                                attaATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCgg                                              <  1:1068685/100‑1 (MQ=255)

                                                  taATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTc                                            <  1:2232063/100‑1 (MQ=255)

                                                  taATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTc                                            >  1:568249/1‑100 (MQ=255)

                                                      atGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCttt                                        >  2:1004748/1‑100 (MQ=255)

                                                      atGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCttt                                        <  1:1647641/100‑1 (MQ=255)

                                                        gAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTgg                                      <  1:2135115/100‑1 (MQ=255)

                                                        gAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTgg                                      <  1:2380506/100‑1 (MQ=255)

                                                         aaaCACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGt                                     >  2:492003/1‑100 (MQ=255)

                                                         aaaCACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGt                                     >  1:1698231/1‑100 (MQ=255)

                                                             acgtgtaCTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCAtt                                 <  1:1253443/95‑1 (MQ=255)

                                                             acTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCAtt                                 <  1:2724996/100‑1 (MQ=255)

                                                              cTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTc                                >  2:934723/1‑100 (MQ=255)

                                                              cTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTc                                >  1:2712782/1‑100 (MQ=255)

                                                              cTTATACTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTc                                <  2:1276913/100‑1 (MQ=255)

                                                                tataCTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCaa                              >  2:526972/1‑100 (MQ=255)

                                                                  taCTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAAtt                            <  1:2859724/100‑1 (MQ=255)

                                                                  taCTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAAtt                            <  1:894498/100‑1 (MQ=255)

                                                                   aCTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTg                           >  2:1032551/1‑100 (MQ=255)

                                                                   aCTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTg                           >  1:729285/1‑100 (MQ=255)

                                                                   aCTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTg                           >  2:346969/1‑100 (MQ=255)

                                                                    cTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGc                          <  1:1675720/100‑1 (MQ=255)

                                                                    cTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGc                          >  2:1868724/1‑100 (MQ=255)

                                                                    cTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGc                          >  2:735102/1‑100 (MQ=255)

                                                                    cTGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGc                          >  2:557972/1‑100 (MQ=255)

                                                                     tGGAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCt                         <  2:924124/100‑1 (MQ=255)

                                                                       gAAACAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTc                       >  2:898429/1‑100 (MQ=255)

                                                                          aCAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAgg                    <  1:9288/100‑1 (MQ=255)

                                                                           cAGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGa                   <  2:1425846/100‑1 (MQ=255)

                                                                            aGGCTGGAATAATCTTAGCCGG‑AAAACGAATGTC‑AAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAAc                 <  2:1466769/100‑1 (MQ=255)

                                                                             ggCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAAc                 >  1:2215669/1‑100 (MQ=255)

                                                                             ggCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAAc                 <  2:2215656/100‑1 (MQ=255)

                                                                             ggCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAAc                 >  1:2338927/1‑100 (MQ=255)

                                                                              gCTGGAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACa                <  1:931936/100‑1 (MQ=255)

                                                                                 ggAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACatat             <  2:2754959/100‑1 (MQ=255)

                                                                                  gAATAATCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACAtata            >  2:2195660/1‑100 (MQ=255)

                                                                                    ataatCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAAc          >  1:2258470/1‑100 (MQ=255)

                                                                                    ataatCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAAc          <  1:1402368/100‑1 (MQ=255)

                                                                                    ataatCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAAc          <  1:188830/100‑1 (MQ=255)

                                                                                    ataatCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAAc          >  1:2259196/1‑100 (MQ=255)

                                                                                    ataatCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAAc          >  1:2797909/1‑100 (MQ=255)

                                                                                    ataatCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAAc          <  1:557974/100‑1 (MQ=255)

                                                                                        tCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATa      <  1:2012767/100‑1 (MQ=255)

                                                                                        tCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATa      <  1:1440701/100‑1 (MQ=255)

                                                                                        tCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATa      <  2:2100790/100‑1 (MQ=255)

                                                                                        tCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATa      <  1:1072099/100‑1 (MQ=255)

                                                                                        tCTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGCCCTTTGGTCATTCTATTGCTTCAGGAACATATAACGAt       >  1:1218399/1‑99 (MQ=255)

                                                                                         cTTAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATaa     >  2:2631670/1‑100 (MQ=255)

                                                                                          ttAGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAg    <  1:2551569/100‑1 (MQ=255)

                                                                                            aGCCGG‑AAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGga  <  2:2296754/100‑1 (MQ=255)

ACTTTTCCTTACAACAAACAGGGCGCTCAATGAGTGCCCTGTCTCTTTATTAATATGAAACACTTATACTGGAAACAGGCTGGAATAATCTTAGCCGGAAAAACGAATGTCAAAAGGCACAGTAAAGATGCCAGTCAGCGTGTTTCCGGTCCTTTGGTCATTCAATTGCTTCAGGAACATATAACGATAAGGA  >  NC_000913/4227526‑4227718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`