Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,410,053 (T)9→8 intergenic (+47/‑80) rlmB → / → yjfI 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,410,0450T.95.1% 400.5 / 14.0 103intergenic (+39/‑88)rlmB/yjfI23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein
Reads supporting (aligned to +/- strand):  ref base T (3/2);  new base . (55/42);  total (59/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 127 total aligned reads displayed.

CCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGAC  >  NC_000913/4409946‑4410137
                                                                                                   |                                                                                            
ccTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGt                                                                                              <  1:895717/100‑1 (MQ=255)
 cTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTGTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtt                                                                                             >  1:2029294/1‑100 (MQ=255)
  tGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAAATCTGGATGGctt                                                                                            <  2:559714/100‑1 (MQ=255)
    aaCGGTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttt                                                                                            <  2:655692/98‑1 (MQ=255)
     aCGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAAATCTGGATGGcttttc                                                                                         <  1:2297998/100‑2 (MQ=39)
      cGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTTTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttt                                                                                        <  2:348049/100‑1 (MQ=255)
      cGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCGGATTTGGATGGttttttt                                                                                        >  2:162191/1‑100 (MQ=255)
      cGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttt                                                                                        >  1:1857297/1‑100 (MQ=255)
      cGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttt                                                                                        <  1:1448827/100‑1 (MQ=255)
      cGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttt                                                                                        <  2:2150055/100‑1 (MQ=255)
      cGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttt                                                                                        >  2:2725321/1‑100 (MQ=255)
      cGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGGcttttca                                                                                        <  1:600499/100‑3 (MQ=38)
      cGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGGcttttca                                                                                        <  1:1901573/100‑3 (MQ=38)
        tttCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGCAAAGCCATCCAGATTTGCATGGttttttttg                                                                                      <  1:1950843/100‑2 (MQ=255)
        tttCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttg                                                                                      <  2:658648/100‑2 (MQ=255)
          tCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttt                                                                                      <  2:907482/98‑1 (MQ=255)
          tCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgtc                                                                                    >  1:2591837/1‑97 (MQ=255)
          tCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgtc                                                                                    <  1:1836830/100‑4 (MQ=255)
           cGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgtct                                                                                   >  1:1284598/1‑96 (MQ=255)
            ggTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCta                                                                                 >  1:1667543/1‑100 (MQ=255)
             gTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCtat                                                                                <  2:2280147/100‑1 (MQ=255)
              ttGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGGcttttcataattctg                                                                                <  1:541601/100‑11 (MQ=25)
               tGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAg                                                                              <  1:800383/100‑1 (MQ=255)
                  gACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTg                                                                           <  1:1152136/100‑1 (MQ=255)
                        aaTTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGa                                                                     <  1:1867754/100‑1 (MQ=255)
                           ttGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATaa                                                                  <  1:2147610/100‑1 (MQ=255)
                           ttGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATaa                                                                  >  1:1277791/1‑100 (MQ=255)
                             gCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAAtt                                                                >  1:124038/1‑100 (MQ=255)
                             gCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAAtt                                                                <  2:2283574/100‑1 (MQ=255)
                              cTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATACAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTa                                                               <  2:386203/100‑1 (MQ=255)
                                tATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACg                                                             >  2:1217991/1‑100 (MQ=255)
                                 aTTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGt                                                            >  2:2579121/1‑100 (MQ=255)
                                  tttGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTa                                                           >  2:1414097/1‑100 (MQ=255)
                                    tGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTAtt                                                         >  2:737715/1‑100 (MQ=255)
                                      aaGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGc                                                       <  2:662716/100‑1 (MQ=255)
                                       aGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTAGTAAGATAATTACGTATTGCa                                                      <  2:143075/100‑1 (MQ=255)
                                        gCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCaa                                                     <  2:760109/100‑1 (MQ=255)
                                           gTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTACGATAATTACGTATTACCAata                                                  >  1:2445082/1‑100 (MQ=255)
                                              cgcCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTc                                               >  2:610911/1‑100 (MQ=255)
                                               gcCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCa                                              >  1:2821969/1‑100 (MQ=255)
                                               gcCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCa                                              <  1:2349632/100‑1 (MQ=255)
                                               gcCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCa                                              <  2:1076594/100‑1 (MQ=255)
                                                ccAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAt                                             >  1:463944/1‑100 (MQ=255)
                                                 cAGCGCAGCTAATTTCTCAGATTTATGAAAAGCCATCCAGATTTGTATGGTTTTTTTTTTTTATAGCTGGTAAGATAATTACGTATCGCAAat                                                   <  2:1756247/93‑1 (MQ=255)
                                                 cAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAtt                                            <  1:1330464/100‑1 (MQ=255)
                                                  aGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAttt                                           >  1:84600/1‑100 (MQ=255)
                                                   gcgcAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAtttt                                          <  2:1002143/100‑1 (MQ=255)
                                                    cgccgttAATTTCTTATAATTATGAAAATCCATCCAGATTTGTATGGTTTTTGTTGTCTATAGCTGCTAAGATAATTACGTATTGCAAat                                                   <  2:2591818/84‑1 (MQ=255)
                                                    cgcAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTtta                                         <  2:967470/100‑1 (MQ=255)
                                                      cAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttatt                                       >  2:383567/1‑100 (MQ=255)
                                                          tAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAata                                   <  2:2103651/100‑1 (MQ=255)
                                                          tAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTGTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAat                                    <  1:1359670/99‑1 (MQ=255)
                                                           aaTTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAatat                                  >  1:1215678/1‑100 (MQ=255)
                                                              ttCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTa                               >  2:1817789/1‑100 (MQ=255)
                                                               tctcATAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTaa                              <  2:739441/100‑1 (MQ=255)
                                                               tctcAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTaa                              >  1:164816/1‑100 (MQ=255)
                                                                ctcAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTaaa                             <  1:2121114/100‑1 (MQ=255)
                                                                 tcAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTATGTATTGCAAATATTCATTTTATTAATATTTaaa                             <  2:2011458/99‑1 (MQ=255)
                                                                 tcAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAAc                            <  1:1728184/100‑1 (MQ=255)
                                                                  cAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTTTATTTTTGGTAAGATAATTACGTATTGTAAATATTCATTTTATTAATATTTtaatt                           >  2:2675551/1‑98 (MQ=255)
                                                                  cAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATATCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACt                           >  2:1866825/1‑100 (MQ=255)
                                                                  cAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTaaa                             >  2:2675758/1‑98 (MQ=255)
                                                                  cAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACt                           <  2:1498446/100‑1 (MQ=255)
                                                                   aGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACtt                          <  1:2548410/100‑1 (MQ=255)
                                                                    gAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTa                         >  2:1244321/1‑100 (MQ=255)
                                                                     aaTTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTaa                        <  1:1464865/100‑1 (MQ=255)
                                                                      gacaaaaaaaACCATCCAAATCTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAAt                       >  1:2242340/7‑100 (MQ=37)
                                                                      gacaaaaaaaACCATCCAAATCTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAAt                       >  1:443967/7‑100 (MQ=37)
                                                                      gacaaaaaaaACCATCCAAATCTGGATGGCTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAAt                       >  2:2256998/7‑100 (MQ=25)
                                                                       cgatctaaaaccATCCAAATCTGGATGGCTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATc                      <  1:508106/90‑1 (MQ=37)
                                                                       acaaaaaaaACCGTCCAAATCTGGATGGCTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATc                      >  1:2204212/6‑100 (MQ=25)
                                                                        caaaaaaaACCATCCAAATCTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCt                     >  2:2851762/5‑100 (MQ=37)
                                                                         aTGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCta                    >  1:1408706/1‑100 (MQ=255)
                                                                          tGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCtat                   >  2:1113356/1‑100 (MQ=255)
                                                                          aaaaaaACCATCCAAATCTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCtat                   >  2:1648024/3‑100 (MQ=38)
                                                                          aaaaaaACCATCCAAATCTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCtat                   >  1:1386526/3‑100 (MQ=38)
                                                                          aaaaaaACCATCCAAATCTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCtat                   >  2:1529966/3‑100 (MQ=38)
                                                                          aaaaaaACCATCCAAATCTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCtat                   >  2:282563/3‑100 (MQ=38)
                                                                           gAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCtat                   >  1:884749/1‑100 (MQ=255)
                                                                           gAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCtata                  >  1:708753/1‑100 (MQ=255)
                                                                           aaaaaACCATCCAAATCTGGATGGCTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCtat                   >  2:2797955/2‑99 (MQ=37)
                                                                           aaaaaACCATCCAAATCTGGATGGCTTTTTTTGTCTATAGATGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCtata                  >  2:2797218/2‑100 (MQ=37)
                                                                             aaaaCCATCCAAATCTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATg                >  2:679370/1‑100 (MQ=255)
                                                                             aaaaCCATCCAAATCTGGATGGCTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATcagatcg                >  2:508104/1‑94 (MQ=37)
                                                                             aaaGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATg                <  2:553411/100‑1 (MQ=255)
                                                                             aaaGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATg                <  1:1917957/100‑1 (MQ=255)
                                                                             aaaGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATg                <  1:2246147/100‑1 (MQ=255)
                                                                              aaaccATCCAAATCTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATgg               >  1:2316561/4‑100 (MQ=255)
                                                                              aaGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATgg               <  2:1277786/100‑1 (MQ=255)
                                                                                 ccttacAGATTTGTATGTTTTTTTTTGTGTATAGTTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATGTAAACTTAATCTATATGGa              <  2:2543538/93‑1 (MQ=255)
                                                                                 ccATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaat            <  2:1991006/100‑1 (MQ=255)
                                                                                   aTCCAGATTTGGATGGTTTTTTTTTTTTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaataa          >  2:827883/1‑100 (MQ=255)
                                                                                   aTCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaataa          >  2:2235115/1‑100 (MQ=255)
                                                                                     ccAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAataata        <  2:1099005/100‑1 (MQ=255)
                                                                                      naGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatat       >  1:1138836/2‑100 (MQ=255)
                                                                                      cAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatat       <  2:2544948/100‑1 (MQ=255)
                                                                                       aGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatata      <  2:722435/100‑1 (MQ=255)
                                                                                        gATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatat       <  2:1484542/98‑1 (MQ=255)
                                                                                         aTTTGGATGG‑‑TTTTTTTGTCTATAGCTGGCAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGa   <  1:1651792/100‑1 (MQ=255)
                                                                                           ttGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGAc  <  2:1667530/100‑1 (MQ=255)
                                                                                                   |                                                                                            
CCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGAC  >  NC_000913/4409946‑4410137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: