BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,503,169	(G)_5→4	coding (263/1329 nt)	insG ←	IS4 transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,503,165	0	G	.	98.0%	431.5 / 1.3	99	coding (267/1329 nt)	insG	IS4 transposase
Reads supporting (aligned to +/- strand): ref base G (1/1); new base . (49/48); total (50/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 106 total aligned reads displayed.

ATGCGGCGTGGCGTTATGCCAGAGCTGCGCTGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACTGGGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCTCAAGCGCCATGCCAACAA  >  NC_000913/4503069‑4503259

aTGCGGCGTGGCGTTATGCCAGAGCTGCGCTGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACTgggg                                                                                             <  1:2281104/100‑1 (MQ=255)

aTGCGGCGTGGCGTTATGCCAGAGCTGCGCTGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACTgggg                                                                                             <  2:487659/100‑1 (MQ=255)

 tGCGGCGTGGCGTTATGCCAGAGCTGCGCTGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACTggggc                                                                                            >  2:517637/1‑99 (MQ=255)

  gcggcgTGGCGTTATGCCAGAGCTGCGCTGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACTggggca                                                                                           >  2:2430347/1‑98 (MQ=255)

   cggcgTGGCGTTATGCCAGAGCTGCGCTGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACTggggcaa                                                                                          >  1:356596/1‑97 (MQ=255)

    ggcgtggcgtTATGCCAGAGCTGCGCTGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACTggggcaac                                                                                         <  1:2548965/100‑5 (MQ=255)

      cgtggcgtTATGCCAGAGCTGCGCTGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGa                                                                                      >  1:39190/1‑100 (MQ=255)

            gtTATGCCAGAGCTGCGCTGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGc                                                                                <  1:1528326/100‑1 (MQ=255)

             ttATGCCAGAGCTGCGCTGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCg                                                                               <  1:1751245/100‑1 (MQ=255)

                tGCCAGAGCTGCGCTGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGAtt                                                                            >  1:495812/1‑100 (MQ=255)

                 gCCAGAGCTGCGCTGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTg                                                                           >  2:2800843/1‑100 (MQ=255)

                  ccAGAGCTGCGCTGTTTTCGTGAACACGTGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGc                                                                          >  1:1530542/1‑100 (MQ=255)

                  ccAGAGCTGCGCTGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGc                                                                          >  1:1530488/1‑100 (MQ=255)

                  ccAGAGCTGCGCTGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGc                                                                          >  2:1079209/1‑100 (MQ=255)

                   cAGAGCTGCGCTGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGcc                                                                         >  1:108342/1‑100 (MQ=255)

                    agagCTGCGCTGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGccc                                                                        <  2:2843584/100‑1 (MQ=255)

                    agagCTGCGCTGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGccc                                                                        <  1:2424280/100‑1 (MQ=255)

                       gCTGCGCTGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGgc                                                                     >  1:1963781/1‑100 (MQ=255)

                        cTGCGCTGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGgca                                                                    <  1:619537/100‑1 (MQ=255)

                          gcgcTGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGgcagc                                                                  >  1:1654326/1‑100 (MQ=255)

                          gcgcTGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGgcagc                                                                  >  2:1632869/1‑100 (MQ=255)

                          gcgcTGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGgcagc                                                                  >  2:1777571/1‑100 (MQ=255)

                            gcTGTTTTCGTGAACACGCGGCGGACAGCCTCAGTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGAGCAACGAAGGGGCGATTGCCCGGCAGCat                                                                >  2:1745691/1‑100 (MQ=255)

                             cTGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCatg                                                               >  1:346826/1‑100 (MQ=255)

                             cTGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGCCAGCatg                                                               >  2:71110/1‑100 (MQ=255)

                              tGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGGATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCatga                                                              <  2:2450299/100‑1 (MQ=255)

                              tGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCatga                                                              <  1:2630132/100‑1 (MQ=255)

                               gTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCatgat                                                             >  1:906558/1‑100 (MQ=255)

                               gTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCatgat                                                             >  2:2655452/1‑100 (MQ=255)

                               gTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCatgat                                                             >  1:1898609/1‑100 (MQ=255)

                                ttttCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCatgatg                                                            <  1:2666600/100‑1 (MQ=255)

                                 tttCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGt                                                           >  1:373369/1‑100 (MQ=255)

                                   tCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTcc                                                         <  2:1825708/100‑1 (MQ=255)

                                     gTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAg                                                       <  1:2017518/100‑1 (MQ=255)

                                       gAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGc                                                     >  2:379635/1‑100 (MQ=255)

                                       gAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGc                                                     <  2:101513/100‑1 (MQ=255)

                                         acacGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGa                                                   >  2:2120387/1‑100 (MQ=255)

                                         acacGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGa                                                   <  1:1444704/100‑1 (MQ=255)

                                         acacGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGa                                                   >  2:2456906/1‑100 (MQ=255)

                                          cacGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGAt                                                  <  1:1401439/100‑1 (MQ=255)

                                          cacGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGAt                                                  >  1:34552/1‑100 (MQ=255)

                                          cacGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGAt                                                  >  1:786467/1‑100 (MQ=255)

                                           acGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGAtt                                                 <  1:1964720/100‑1 (MQ=255)

                                             gcggcgGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTca                                               <  2:1654313/100‑1 (MQ=255)

                                              cggcggACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTcac                                              <  1:2430363/100‑1 (MQ=255)

                                              cggcggACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTcac                                              >  1:1589891/1‑100 (MQ=255)

                                                    aCAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTg                                        <  1:2426897/100‑1 (MQ=255)

                                                      aGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATACCGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGt                                      <  1:379135/100‑1 (MQ=255)

                                                      aGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGt                                      <  1:1063910/100‑1 (MQ=255)

                                                      aGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGt                                      <  1:745715/100‑1 (MQ=255)

                                                      aGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGt                                      <  2:1293666/100‑1 (MQ=255)

                                                       gCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTg                                     >  1:2580861/1‑100 (MQ=255)

                                                       gCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTg                                     >  1:1680921/1‑100 (MQ=255)

                                                       gCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTg                                     >  1:1067515/1‑100 (MQ=255)

                                                       gCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTg                                     <  1:1484093/100‑1 (MQ=255)

                                                        ccTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGa                                    <  2:2732961/100‑1 (MQ=255)

                                                        ccTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGa                                    >  2:1382328/1‑100 (MQ=255)

                                                         cTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGaa                                   >  1:1233826/1‑100 (MQ=255)

                                                          tCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAag                                  >  1:2878466/1‑100 (MQ=255)

                                                          tCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAag                                  <  1:2644464/100‑1 (MQ=255)

                                                           cACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAaga                                 <  2:108342/100‑1 (MQ=255)

                                                           cACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAaga                                 <  2:184647/100‑1 (MQ=255)

                                                            aCTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAagag                                <  2:2382524/100‑1 (MQ=255)

                                                            aCTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAagag                                >  2:2829226/1‑100 (MQ=255)

                                                             cTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAgg                               >  2:32530/1‑100 (MQ=255)

                                                              ttCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGt                              <  2:1234559/100‑1 (MQ=255)

                                                               tCCCAGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGtt                             >  1:1443609/1‑100 (MQ=255)

                                                               acccAGGCGCTGGCGGGCCTGAATAACGGCACTGGGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAgg                               <  1:2102467/98‑1 (MQ=255)

                                                                  cAGGCGCTGGCGGGCCTGAATAACGGCACT‑‑GGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGGTTCACAATCTGGTGAAGAGGTTCttt                         >  2:1544009/1‑100 (MQ=255)

                                                                   aGGCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCttt                         <  2:2795560/100‑1 (MQ=255)

                                                                    ggCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCtt                          <  2:600341/98‑1 (MQ=255)

                                                                    ggCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTa                        <  2:34552/100‑1 (MQ=255)

                                                                    ggCGCTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTa                        <  1:517639/100‑1 (MQ=255)

                                                                      cgcTGGCGGGCCTGAATAACGGCACTGGGGGCAACGAAGGGGCGATTGTCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTAc                       >  1:292163/1‑100 (MQ=255)

                                                                       gcTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGc                     <  2:1606041/100‑1 (MQ=255)

                                                                        cTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCt                    <  1:1114403/100‑1 (MQ=255)

                                                                        cTGGCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCt                    <  2:2103664/100‑1 (MQ=255)

                                                                          ggCGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCTCa                  <  2:65813/100‑1 (MQ=255)

                                                                            cGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCTCAAg                >  1:287975/1‑100 (MQ=255)

                                                                            cGGGCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCTCAAg                >  1:223547/1‑100 (MQ=255)

                                                                               gCCTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCTCAAgcgc             >  1:1929594/1‑100 (MQ=255)

                                                                                ccTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGTAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCTCAAGCGcc            >  2:1891470/1‑100 (MQ=255)

                                                                                ccTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCTCAAGCGcc            >  1:392408/1‑100 (MQ=255)

                                                                                ccTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCTCAAGCGcc            <  2:2580842/100‑1 (MQ=255)

                                                                                 cTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCTCAAGCGCCa           <  1:2196218/100‑1 (MQ=255)

                                                                                 cTGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCTCAAGCGCCa           <  2:1446112/100‑1 (MQ=255)

                                                                                  tGAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCTCAAGCGCCAt          >  2:1106487/1‑100 (MQ=255)

                                                                                   gAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCTCAAGCGCCATg         <  2:1067513/100‑1 (MQ=255)

                                                                                   gAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCTCAAGCGCCATg         <  1:2163801/100‑1 (MQ=255)

                                                                                   gAATAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCTCAAGCGCCATg         >  1:103915/1‑100 (MQ=255)

                                                                                    aaTAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCTCAAGCGCCATGc        <  2:2632339/100‑1 (MQ=255)

                                                                                     aTAACGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCTCAAGCGCCATGcc       >  1:2850047/1‑100 (MQ=255)

                                                                                      tagcGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCTCAAGCGCCATGcc       <  1:2045757/96‑1 (MQ=255)

                                                                                        aCGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCTCAAGCGCCATGCcaac    <  2:1421820/100‑1 (MQ=255)

                                                                                        aCGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCTCAAGCGCCATGCcaac    >  1:2210053/1‑100 (MQ=255)

                                                                                         cGGCACT‑GGGTCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGTAGTGGTTCTTTACGCTCAAGCGCCATGCcaaca   <  2:1898679/100‑1 (MQ=255)

                                                                                         cGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCTCAAGCGCCATGCcaaca   >  1:1977579/1‑100 (MQ=255)

                                                                                         cGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCTCAAGCGCCATGCcaaca   >  1:2497562/1‑100 (MQ=255)

                                                                                         cGGCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCTCAAGCGCCATGCcaac    <  2:2878445/99‑1 (MQ=255)

                                                                                          ggCACT‑GGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCTCAAGCGCCATGCcaacaa  <  1:938885/100‑1 (MQ=255)

ATGCGGCGTGGCGTTATGCCAGAGCTGCGCTGTTTTCGTGAACACGCGGCGGACAGCCTCACTTCCCAGGCGCTGGCGGGCCTGAATAACGGCACTGGGGGCAACGAAGGGGCGATTGCCCGGCAGCATGATGTCCAGGCGATTCACAATCTGGTGAAGAGGTTCTTTACGCTCAAGCGCCATGCCAACAA  >  NC_000913/4503069‑4503259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`